Restriction map for
pFN21AB9340
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# NO CUT ENZYMES ( 117 enzymes) #
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Acc65I AccI AccII AccIII AclI
AgeI AhdI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AvaI AvrII BbeI BbrPI BbvCI
BciVI BlnI BsaAI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmFI
BsoBI BspEI BspHI BsrBI BsrFI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstZ17I Bsu36I Cfr10I CpoI
DraI DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FseI HaeII HgaI HpaI
KasI KpnI Ksp632I KspI MluI
MroI MslI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 2044 GACGTC GACGT^C
AciI 1050 GCGG CCGC(-3/-1)
AcyI 2044 GACGTC G[GA]^CG[CT]C
AlwNI 410 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 2133 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1515 TGCGCA TGC^GCA
BalI 266 TGGCCA TGG^CCA
BamHI 1667 GGATCC G^GATCC
BbsI 1240 GAAGAC GAAGAC(2/6)
BcgI 1737 GCATCATTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2265 TGATCA T^GATCA
BcnI 1058 CCCGG CC^[GC]GG
BglI 213 GCCACAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 63 AGATCT A^GATCT
BlpI 1639 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1639 GCTGAGC GC^T[ACGT]AGC
BpuAI 1240 GAAGAC GAAGAC(2/6)
BsaBI 2263 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2044 GACGTC G[GA]^CG[CT]C
BsgI 1938 GTGCAG GTGCAG(16/14)
Bsp1407I 2021 TGTACA T^GTACA
BspDI 1558 ATCGAT AT^CGAT
BsrDI 925 GCAATG GCAATG(2/0)
BsrGI 2021 TGTACA T^GTACA
BstAPI 1026 GCATCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1400 TTCGAA TT^CGAA
BstXI 1470 CCAGGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1639 GCTGAGC GC^T[ACGT]AGC
ClaI 1558 ATCGAT AT^CGAT
DraII 1541 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 266 TGGCCA [CT]^GGCC[GA]
EcoO109I 1541 AGGCCCT [GA]G^G[ACGT]CC[CT]
FbaI 2265 TGATCA T^GATCA
FspI 1515 TGCGCA TGC^GCA
HapII 1059 CCGG C^CGG
Hin1I 2044 GACGTC G[GA]^CG[CT]C
HincII 664 GTCAAC GT[CT]^[GA]AC
HindII 664 GTCAAC GT[CT]^[GA]AC
HpaII 1059 CCGG C^CGG
MamI 2263 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1598 CAATTG C^AATTG
MluNI 266 TGGCCA TGG^CCA
MscI 266 TGGCCA TGG^CCA
MspI 1059 CCGG C^CGG
MunI 1598 CAATTG C^AATTG
NciI 1058 CCCGG CC^[GC]GG
NspV 1400 TTCGAA TT^CGAA
PflFI 2133 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 574 CAGCTG CAG^CTG
SexAI 2229 ACCAGGT A^CC[AT]GGT
SfuI 1400 TTCGAA TT^CGAA
SspBI 2021 TGTACA T^GTACA
SspI 766 AATATT AAT^ATT
Tth111I 2133 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AflII 886 CTTAAG C^TTAAG
1075 CTTAAG
AflIII 1346 ACATGT A^C[GA][CT]GT
2024 ACATGT
Alw44I 251 GTGCAC G^TGCAC
2149 GTGCAC
ApaLI 251 GTGCAC G^TGCAC
2149 GTGCAC
AvaII 579 GGACC G^G[AT]CC
2236 GGTCC
BanI 876 GGCACC G^G[CT][GA]CC
2032 GGTGCC
BanII 1655 GAGCCC G[GA]GC[CT]^C
2051 GAGCCC
BfrI 886 CTTAAG C^TTAAG
1075 CTTAAG
BsmI 824 GAATGC GAATGC(1/-1)
2293 GAATGC
BspLU11I 1346 ACATGT A^CATGT
2024 ACATGT
BspMI 15 ACCTGC ACCTGC(4/8)
2111 ACCTGC
Cac8I 1159 GCAAGC GC[ACGT]^[ACGT]GC
1503 GCAGGC
CfoI 1516 GCGC GCG^C
2105 GCGC
DraIII 376 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
2228 CACCAGGTG
HhaI 1516 GCGC GCG^C
2105 GCGC
HinP1I 1516 GCGC G^CGC
2105 GCGC
HindIII 350 AAGCTT A^AGCTT
1982 AAGCTT
MaeII 954 ACGT A^CGT
2045 ACGT
MaeIII 1684 GTGAC ^GT[ACGT]AC
2215 GTCAC
MspA1I 574 CAGCTG C[AC]G^C[GT]G
1048 CAGCGG
PstI 1501 CTGCAG CTGCA^G
2162 CTGCAG
SmlI 886 CTTAAG C^T[CT][GA]AG
1075 CTTAAG
Tsp45I 1684 GTGAC ^GT[GC]AC
2215 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I AspHI BbvI BfaI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MboI MboII MflI MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XcmI XhoII XmnI