Restriction map for
pFN21AB9340
(Circular) (Six-base) MAPSORT of: pFN21AB9340.seq Check: 2181 from: 1 to: 7003
pFN21AB9340 7003 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4053 4813 278
Size: 53 83 186 936 2517 760 2468
Fragments arranged by size:
2517 2468 936 760 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4346 1937
Size: 2409 4594
Acc65I G'GTAC_C
Cuts at: 4324 4324
Size: 7003
AclI AA'CG_TT
Cuts at: 1563 5130 5503 1563
Size: 3567 373 3063
Fragments arranged by size:
3567 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2855 3300 3577 3938 4205 5066 6450 6718
Size: 1223 445 277 361 267 861 1384 268
Cuts at: 6718 1632
Size: 1917
Fragments arranged by size:
1917 1384 1223 861 445 361 277 268
267
AflII C'TTAA_G
Cuts at: 820 1017 2891 3080 820
Size: 197 1874 189 4743
Fragments arranged by size:
4743 1874 197 189
AflIII A'CryG_T
Cuts at: 1203 3351 4029 5902 6879 1203
Size: 2148 678 1873 977 1327
Fragments arranged by size:
2148 1873 1327 977 678
AgeI A'CCGG_T
Cuts at: 5834 5834
Size: 7003
AhdI GACnn_n'nnGTC
Cuts at: 5732 5732
Size: 7003
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7003
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4179 1184
Size: 2995 4008
AlwNI CAG_nnn'CTG
Cuts at: 2420 4746 6318 2420
Size: 2326 1572 3105
Fragments arranged by size:
3105 2326 1572
ApaLI G'TGCA_C
Cuts at: 2256 4154 5060 6216 2256
Size: 1898 906 1156 3043
Fragments arranged by size:
3043 1898 1156 906
ApoI r'AATT_y
Cuts at: 1493 2801 2991 3374 3395 3897 4312 4538 4690
Size: 1308 190 383 21 502 415 226 152
Cuts at: 4690 6799 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1308 502 415 383 226 190
152 21
AseI AT'TA_AT
Cuts at: 160 1032 5557 160
Size: 872 4525 1606
Fragments arranged by size:
4525 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7003
AvaI C'yCGr_G
Cuts at: 1870 1943 4328 1870
Size: 73 2385 4545
Fragments arranged by size:
4545 2385 73
BamHI G'GATC_C
Cuts at: 3672 4333 4700 3672
Size: 661 367 5975
Fragments arranged by size:
5975 661 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2881 4037 4324 5779 618
Size: 325 217 627 1094 1156 287 1455 1842
Fragments arranged by size:
1842 1455 1156 1094 627 325 287 217
BanII G_rGCy'C
Cuts at: 1391 3664 4060 4322 1391
Size: 2273 396 262 4072
Fragments arranged by size:
4072 2273 396 262
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3252 928
Size: 971 1353 4679
Fragments arranged by size:
4679 1353 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3731 3765 5194 5228 1903
Size: 34 1794 34 1429 34 3678
Fragments arranged by size:
3678 1794 1429 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4895 6105 4895
Size: 1210 5793
BclI T'GATC_A
Cuts at: 1548 4270 1548
Size: 2722 4281
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2224 5614 136
Size: 107 122 71 631 605 552 3390 1525
Fragments arranged by size:
3390 1525 631 605 552 122 107 71
BglII A'GATC_T
Cuts at: 2068 6998 2068
Size: 4930 2073
BlpI GC'TnA_GC
Cuts at: 3645 4420 3645
Size: 775 6228
Bme1580I G_kGCm'C
Cuts at: 1790 2260 3372 4042 4158 5064 6220 1790
Size: 470 1112 670 116 906 1156 2573
Fragments arranged by size:
2573 1156 1112 906 670 470 116
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7003
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2049 2486 2962
Size: 416 53 83 399 24 632 437 476
Cuts at: 2962 4135 4734 5692 442
Size: 1173 599 958 1753
Fragments arranged by size:
1753 1173 958 632 599 476 437 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7003
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2463 2594 2840 5663 1239
Size: 1224 131 246 2823 2579
Fragments arranged by size:
2823 2579 1224 246 131
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5128 5993 6291 5128
Size: 865 298 5840
Fragments arranged by size:
5840 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5666 882
Size: 617 4167 2219
Fragments arranged by size:
4167 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4794 493
Size: 711 3590 2702
Fragments arranged by size:
3590 2702 711
BsaBI GATnn'nnATC
Cuts at: 4272 6997 4272
Size: 2725 4278
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4050 4810 5192 5827
Size: 53 83 186 936 2517 760 382 635
Cuts at: 5827 275
Size: 1451
Fragments arranged by size:
2517 1451 936 760 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5436 5834 6108 6255 6666 5436
Size: 398 274 147 411 5773
Fragments arranged by size:
5773 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2184 2214 2895 2925 1178
Size: 30 165 30 781 30 681 30 5256
Fragments arranged by size:
5256 781 681 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2192 2327 2330 1186
Size: 1006 135 3 5859
Fragments arranged by size:
5859 1006 135 3
BseYI C'CCAG_C
Cuts at: 2208 6206 6702 2208
Size: 3998 496 2509
Fragments arranged by size:
3998 2509 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3964 1163
Size: 573 2228 4202
Fragments arranged by size:
4202 2228 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4453 5214 6242 1540
Size: 275 186 2452 761 1028 2301
Fragments arranged by size:
2452 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2260 2499 4158 5064 5149 6220 2260
Size: 239 1659 906 85 1071 3043
Fragments arranged by size:
3043 1659 1071 906 239 85
BsmI GAATG_Cn'
Cuts at: 2835 4304 4514 4607 2835
Size: 1469 210 93 5231
Fragments arranged by size:
5231 1469 210 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2260 2499 3372 3664 4042 4060 4158
Size: 399 470 239 873 292 378 18 98
Cuts at: 4158 4322 5064 5149 6220 1391
Size: 164 742 85 1071 2174
Fragments arranged by size:
2174 1071 873 742 470 399 378 292
239 164 98 85 18
BspEI T'CCGG_A
Cuts at: 6666 6666
Size: 7003
BspHI T'CATG_A
Cuts at: 4892 6622 4892
Size: 1730 5273
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2029 4125 4358 844
Size: 1185 2096 233 3489
Fragments arranged by size:
3489 2096 1185 233
BsrBI CCG'CTC
Cuts at: 1394 1823 4890 1394
Size: 429 3067 3507
Fragments arranged by size:
3507 3067 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2937 5498 5672 65
Size: 1183 1689 2561 174 1396
Fragments arranged by size:
2561 1689 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5647 5834 1700
Size: 110 3837 187 2869
Fragments arranged by size:
3837 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4026 96
Size: 3930 3073
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7003
BssSI C'ACGA_G
Cuts at: 5063 6075 5063
Size: 1012 5991
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3037 794
Size: 2243 4760
BstBI TT'CG_AA
Cuts at: 3406 5807 3406
Size: 2401 4602
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7003
BstXI CCAn_nnnn'nTGG
Cuts at: 3482 3482
Size: 7003
BstYI r'GATC_y
Cuts at: 1972 2068 3288 3672 4333 4700 5085 5102 6554
Size: 96 1220 384 661 367 385 17 1452
Cuts at: 6554 6640 6652 6998 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1220 661 385 384 367 346
96 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5512
Fragments arranged by size:
5512 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6264
Fragments arranged by size:
6264 683 56
BtsI GCAGTG_nn'
Cuts at: 4519 5313 5333 4519
Size: 794 20 6189
Fragments arranged by size:
6189 794 20
ClaI AT'CG_AT
Cuts at: 1554 3564 1554
Size: 2010 4993
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6968
DraI TTT'AAA
Cuts at: 4307 4307
Size: 7003
DraIII CAC_nnn'GTG
Cuts at: 2386 4238 2386
Size: 1852 5151
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6010 809
Size: 507 4694 1802
Fragments arranged by size:
4694 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2271 4450 5339
Size: 54 1425 50 275 18 441 2179 889
Cuts at: 5339 6968 8
Size: 1629 43
Fragments arranged by size:
2179 1629 1425 889 441 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4450 1537
Size: 275 2638 4090
Fragments arranged by size:
4090 2638 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4933 1353
Size: 3580 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5590 5964 6110 387
Size: 1064 138 4001 374 146 1280
Fragments arranged by size:
4001 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2463 2594 2840 2855 3300 3577 3938
Size: 393 831 131 246 15 445 277 361
Cuts at: 3938 4205 5066 5663 6450 6718 1239
Size: 267 861 597 787 268 1524
Fragments arranged by size:
1524 861 831 787 597 445 393 361
277 268 267 246 131 15
EcoO109I rG'GnC_Cy
Cuts at: 3547 3547
Size: 7003
EcoRI G'AATT_C
Cuts at: 4312 4690 4312
Size: 378 6625
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3229 3818 4034 4226 3229
Size: 589 216 192 6006
Fragments arranged by size:
6006 589 216 192
FspI TGC'GCA
Cuts at: 3522 5509 3522
Size: 1987 5016
HaeII r_GCGC'y
Cuts at: 1678 6150 1678
Size: 4472 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2652 2667 2684 2699 3276
Size: 32 135 32 1080 15 17 15 577
Cuts at: 3276 3308 3611 3643 3896 3928 5644 5676 5718
Size: 32 303 32 253 32 1716 32 42
Cuts at: 5718 5750 1373
Size: 32 2626
Fragments arranged by size:
2626 1716 1080 577 303 253 135 42
32 32 32 32 32 32 32 17
15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2671 4347 4593 669
Size: 988 281 733 1676 246 3079
Fragments arranged by size:
3079 1676 988 733 281 246
HindIII A'AGCT_T
Cuts at: 748 2355 3987 748
Size: 1607 1632 3764
Fragments arranged by size:
3764 1632 1607
HpaI GTT'AAC
Cuts at: 4593 4593
Size: 7003
KpnI G_GTAC'C
Cuts at: 4328 4328
Size: 7003
MfeI C'AATT_G
Cuts at: 3603 4602 3603
Size: 999 6004
MluI A'CGCG_T
Cuts at: 6879 6879
Size: 7003
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2694 3755 3810 6117 6301 1305
Size: 1389 1061 55 2307 184 2007
Fragments arranged by size:
2307 2007 1389 1061 184 55
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2273 10
Size: 54 1425 343 441 4740
Fragments arranged by size:
4740 1425 441 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4961 5320 5479 518
Size: 648 3795 359 159 2042
Fragments arranged by size:
3795 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2581 3055 4419 5096 6244 6489 1606
Size: 975 474 1364 677 1148 245 2120
Fragments arranged by size:
2120 1364 1148 975 677 474 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5512
Fragments arranged by size:
5512 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7003
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7003
NotI GC'GGCC_GC
Cuts at: 4450 4450
Size: 7003
NspI r_CATG'y
Cuts at: 1132 1229 3040 3355 3759 4033 4361 5906 1132
Size: 97 1811 315 404 274 328 1545 2229
Fragments arranged by size:
2229 1811 1545 404 328 315 274 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7003
PciI A'CATG_T
Cuts at: 3351 4029 5902 3351
Size: 678 1873 4452
Fragments arranged by size:
4452 1873 678
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7003
PmeI GTTT'AAAC
Cuts at: 4307 4307
Size: 7003
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5072 5104 6585 6617 5072
Size: 32 1481 32 5458
Fragments arranged by size:
5458 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7003
PsiI TTA'TAA
Cuts at: 4573 4573
Size: 7003
PstI C_TGCA'G
Cuts at: 830 3510 4171 4355 830
Size: 2680 661 184 3478
Fragments arranged by size:
3478 2680 661 184
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7003
PvuII CAG'CTG
Cuts at: 2581 2581
Size: 7003
SalI G'TCGA_C
Cuts at: 1936 4345 1936
Size: 2409 4594
SbfI CC_TGCA'GG
Cuts at: 4355 4355
Size: 7003
ScaI AGT'ACT
Cuts at: 5251 6963 5251
Size: 1712 5291
SexAI A'CCwGG_T
Cuts at: 4234 4234
Size: 7003
SfcI C'TryA_G
Cuts at: 826 1045 2470 3156 3506 4167 4351 5486 6167
Size: 219 1425 686 350 661 184 1135 681
Cuts at: 6167 6358 826
Size: 191 1471
Fragments arranged by size:
1471 1425 1135 686 681 661 350 219
191 184
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7003
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7003
SmaI CCC'GGG
Cuts at: 1872 4330 1872
Size: 2458 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2891 3080 5107 6008 6270 820
Size: 197 926 948 189 2027 901 262 1553
Fragments arranged by size:
2027 1553 948 926 901 262 197 189
SnaBI TAC'GTA
Cuts at: 493 4794 493
Size: 4301 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7003
SphI G_CATG'C
Cuts at: 1132 4361 1132
Size: 3229 3774
SspI AAT'ATT
Cuts at: 5 52 2773 4927 5
Size: 47 2721 2154 2081
Fragments arranged by size:
2721 2154 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7003
StyI C'CwwG_G
Cuts at: 513 1065 2004 2532 3977 4103 4263 4442 513
Size: 552 939 528 1445 126 160 179 3074
Fragments arranged by size:
3074 1445 939 552 528 179 160 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2443 5030 5047 5200 5385 5830 325
Size: 1255 863 2587 17 153 185 445 1498
Fragments arranged by size:
2587 1498 1255 863 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3360 4026 5249
Size: 274 80 33 51 1443 1383 666 1223
Cuts at: 5249 6961 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1383 1223 666 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2895 2927 3456 3488 3921 3953 6963 6995 2895
Size: 32 529 32 433 32 3010 32 2903
Fragments arranged by size:
3010 2903 529 433 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4141 1316
Size: 2825 4178
XbaI T'CTAG_A
Cuts at: 4339 4339
Size: 7003
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2281 3379 4075 2281
Size: 1098 696 5209
Fragments arranged by size:
5209 1098 696
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7003
XmaI C'CCGG_G
Cuts at: 1870 4328 1870
Size: 2458 4545
XmnI GAAnn'nnTTC
Cuts at: 2455 2961 2991 3048 5132 2455
Size: 506 30 57 2084 4326
Fragments arranged by size:
4326 2084 506 57 30
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4051 4811 276
Size: 53 83 186 936 2517 760 2468
Fragments arranged by size:
2517 2468 936 760 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI