Restriction map for
pFN21AB9340
(Linear) (Six-base) MAPSORT of: KIBB9340.seq Check: 6272 from: 1 to: 2298
KIBB9340.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AatII G_ACGT'C
Cuts at: 0 2048 2298
Size: 2048 250
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 850 1295 1572 1933 2200 2298
Size: 850 445 277 361 267 98
Fragments arranged by size:
850 445 361 277 267 98
AflII C'TTAA_G
Cuts at: 0 886 1075 2298
Size: 886 189 1223
Fragments arranged by size:
1223 886 189
AflIII A'CryG_T
Cuts at: 0 1346 2024 2298
Size: 1346 678 274
Fragments arranged by size:
1346 678 274
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2174 2298
Size: 2174 124
AlwNI CAG_nnn'CTG
Cuts at: 0 415 2298
Size: 415 1883
ApaLI G'TGCA_C
Cuts at: 0 251 2149 2298
Size: 251 1898 149
Fragments arranged by size:
1898 251 149
ApoI r'AATT_y
Cuts at: 0 796 986 1369 1390 1892 2298
Size: 796 190 383 21 502 406
Fragments arranged by size:
796 502 406 383 190 21
BamHI G'GATC_C
Cuts at: 0 1667 2298
Size: 1667 631
BanI G'GyrC_C
Cuts at: 0 876 2032 2298
Size: 876 1156 266
Fragments arranged by size:
1156 876 266
BanII G_rGCy'C
Cuts at: 0 1659 2055 2298
Size: 1659 396 243
Fragments arranged by size:
1659 396 243
BbsI GAAGACnn'nnnn_
Cuts at: 0 1247 2298
Size: 1247 1051
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1726 1760 2298
Size: 1726 34 538
Fragments arranged by size:
1726 538 34
BclI T'GATC_A
Cuts at: 0 2265 2298
Size: 2265 33
BglI GCCn_nnn'nGGC
Cuts at: 0 219 2298
Size: 219 2079
BglII A'GATC_T
Cuts at: 0 63 2298
Size: 63 2235
BlpI GC'TnA_GC
Cuts at: 0 1640 2298
Size: 1640 658
Bme1580I G_kGCm'C
Cuts at: 0 255 1367 2037 2153 2298
Size: 255 1112 670 116 145
Fragments arranged by size:
1112 670 255 145 116
BmrI ACTGGGnnnn_n'
Cuts at: 0 44 481 957 2130 2298
Size: 44 437 476 1173 168
Fragments arranged by size:
1173 476 437 168 44
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 589 835 2298
Size: 458 131 246 1463
Fragments arranged by size:
1463 458 246 131
BsaBI GATnn'nnATC
Cuts at: 0 2267 2298
Size: 2267 31
BsaHI Gr'CG_yC
Cuts at: 0 2045 2298
Size: 2045 253
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 179 209 890 920 2298
Size: 179 30 681 30 1378
Fragments arranged by size:
1378 681 179 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 187 322 325 2298
Size: 187 135 3 1973
Fragments arranged by size:
1973 187 135 3
BseYI C'CCAG_C
Cuts at: 0 203 2298
Size: 203 2095
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1959 2298
Size: 1959 339
BsiHKAI G_wGCw'C
Cuts at: 0 255 494 2153 2298
Size: 255 239 1659 145
Fragments arranged by size:
1659 255 239 145
BsmI GAATG_Cn'
Cuts at: 0 830 2298
Size: 830 1468
Bsp1286I G_dGCh'C
Cuts at: 0 255 494 1367 1659 2037 2055 2153 2298
Size: 255 239 873 292 378 18 98 145
Fragments arranged by size:
873 378 292 255 239 145 98 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 24 2120 2298
Size: 24 2096 178
Fragments arranged by size:
2096 178 24
BsrDI GCAATG_nn'
Cuts at: 0 932 2298
Size: 932 1366
BsrGI T'GTAC_A
Cuts at: 0 2021 2298
Size: 2021 277
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1032 2298
Size: 1032 1266
BstBI TT'CG_AA
Cuts at: 0 1401 2298
Size: 1401 897
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1477 2298
Size: 1477 821
BstYI r'GATC_y
Cuts at: 0 63 1283 1667 2298
Size: 63 1220 384 631
Fragments arranged by size:
1220 631 384 63
ClaI AT'CG_AT
Cuts at: 0 1559 2298
Size: 1559 739
DraIII CAC_nnn'GTG
Cuts at: 0 381 2233 2298
Size: 381 1852 65
Fragments arranged by size:
1852 381 65
EaeI y'GGCC_r
Cuts at: 0 266 2298
Size: 266 2032
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 589 835 850 1295 1572 1933 2200
Size: 458 131 246 15 445 277 361 267
Cuts at: 2200 2298
Size: 98
Fragments arranged by size:
458 445 361 277 267 246 131 98
15
EcoO109I rG'GnC_Cy
Cuts at: 0 1542 2298
Size: 1542 756
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1224 1813 2029 2221 2298
Size: 1224 589 216 192 77
Fragments arranged by size:
1224 589 216 192 77
FspI TGC'GCA
Cuts at: 0 1517 2298
Size: 1517 781
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 647 662 679 694 1271 1303 1606 1638
Size: 647 15 17 15 577 32 303 32
Cuts at: 1638 1891 1923 2298
Size: 253 32 375
Fragments arranged by size:
647 577 375 303 253 32 32 32
17 15 15
HincII GTy'rAC
Cuts at: 0 666 2298
Size: 666 1632
HindIII A'AGCT_T
Cuts at: 0 350 1982 2298
Size: 350 1632 316
Fragments arranged by size:
1632 350 316
MfeI C'AATT_G
Cuts at: 0 1598 2298
Size: 1598 700
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 689 1750 1805 2298
Size: 689 1061 55 493
Fragments arranged by size:
1061 689 493 55
MscI TGG'CCA
Cuts at: 0 268 2298
Size: 268 2030
MspA1I CmG'CkG
Cuts at: 0 576 1050 2298
Size: 576 474 1248
Fragments arranged by size:
1248 576 474
NspI r_CATG'y
Cuts at: 0 1035 1350 1754 2028 2298
Size: 1035 315 404 274 270
Fragments arranged by size:
1035 404 315 274 270
PciI A'CATG_T
Cuts at: 0 1346 2024 2298
Size: 1346 678 274
Fragments arranged by size:
1346 678 274
PstI C_TGCA'G
Cuts at: 0 1505 2166 2298
Size: 1505 661 132
Fragments arranged by size:
1505 661 132
PvuII CAG'CTG
Cuts at: 0 576 2298
Size: 576 1722
SexAI A'CCwGG_T
Cuts at: 0 2229 2298
Size: 2229 69
SfcI C'TryA_G
Cuts at: 0 465 1151 1501 2162 2298
Size: 465 686 350 661 136
Fragments arranged by size:
686 661 465 350 136
SmlI C'TyrA_G
Cuts at: 0 886 1075 2298
Size: 886 189 1223
Fragments arranged by size:
1223 886 189
SspI AAT'ATT
Cuts at: 0 768 2298
Size: 768 1530
StyI C'CwwG_G
Cuts at: 0 527 1972 2098 2258 2298
Size: 527 1445 126 160 40
Fragments arranged by size:
1445 527 160 126 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 438 2298
Size: 438 1860
TatI w'GTAC_w
Cuts at: 0 1355 2021 2298
Size: 1355 666 277
Fragments arranged by size:
1355 666 277
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 890 922 1451 1483 1916 1948 2298
Size: 890 32 529 32 433 32 350
Fragments arranged by size:
890 529 433 350 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2136 2298
Size: 2136 162
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 276 1374 2070 2298
Size: 276 1098 696 228
Fragments arranged by size:
1098 696 276 228
XmnI GAAnn'nnTTC
Cuts at: 0 450 956 986 1043 2298
Size: 450 506 30 57 1255
Fragments arranged by size:
1255 506 450 57 30
ZraI GAC'GTC
Cuts at: 0 2046 2298
Size: 2046 252
Enzymes that do cut:
AatII AcuI AflII AflIII AlfI AlwNI ApaLI ApoI
BamHI BanI BanII BbsI BcgI BclI BglI BglII
BlpI Bme1580I BmrI BpmI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrGI
BstAPI BstBI BstXI BstYI ClaI DraIII EaeI Eco57MI
EcoO109I FalI FspI Hin4I HincII HindIII MfeI MmeI
MscI MspA1I NspI PciI PstI PvuII SexAI SfcI
SmlI SspI StyI TaqII TatI TstI Tth111I XcmI
XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AgeI AhdI AleI
AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BbeI BbvCI BciVI BfrBI BmgBI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaWI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BsrFI BssHII BssSI BstEII BstZ17I Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EagI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspAI HaeII
HpaI KasI KpnI MluI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI XbaI
XhoI XmaI