Restriction map for
pFN21AB9339
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# NO CUT ENZYMES ( 73 enzymes) #
##################################
Acc65I AclI AflII Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspI AviII AvrII BamHI BbrPI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsiEI BsiWI Bsp1407I
BspDI BsrGI BssHII BssSI BstBI
ClaI CpoI DraI DraIII EagI
EclXI Eco47III Eco52I EcoRV EcoT22I
FbaI FseI FspI HpaI KpnI
MamI MluI NheI NotI NruI
NsiI NspV PacI PflFI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SalI SexAI SfiI SfuI
SgrAI SnaBI SpeI SspBI SspI
SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 1816 GTATAC GT^[AC][GT]AC
AccIII 2963 TCCGGA T^CCGGA
AgeI 1651 ACCGGT A^CCGGT
AocI 3145 CCTGAGG CC^T[ACGT]AGG
ApaI 2504 GGGCCC GGGCC^C
BcgI 3434 GCACACTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1198 CTAG C^TAG
BseAI 2963 TCCGGA T^CCGGA
BspEI 2963 TCCGGA T^CCGGA
BspHI 3308 TCATGA T^CATGA
BspLU11I 3224 ACATGT A^CATGT
Bst1107I 1816 GTATAC GTA^TAC
BstAPI 3623 GCATGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1654 GGTGACC G^GT[ACGT]ACC
BstPI 1654 GGTGACC G^GT[ACGT]ACC
BstZ17I 1816 GTATAC GTA^TAC
Bsu36I 3145 CCTGAGG CC^T[ACGT]AGG
DrdI 792 GACTGGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3145 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1654 GGTGACC G^GT[ACGT]ACC
HindIII 2114 AAGCTT A^AGCTT
KspI 2637 CCGCGG CCGC^GG
MaeI 1198 CTAG C^TAG
MfeI 395 CAATTG C^AATTG
MroI 2963 TCCGGA T^CCGGA
MunI 395 CAATTG C^AATTG
NaeI 2036 GCCGGC GCC^GGC
NcoI 2210 CCATGG C^CATGG
NdeI 1070 CATATG CA^TATG
NgoMIV 2036 GCCGGC G^CCGGC
PinAI 1651 ACCGGT A^CCGGT
PshAI 683 GACGTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3308 TCATGA T^CATGA
SacII 2637 CCGCGG CCGC^GG
SbfI 3525 CCTGCAGG CCTGCA^GG
ScaI 3428 AGTACT AGT^ACT
SmaI 3038 CCCGGG CCC^GGG
SphI 3623 GCATGC GCATG^C
Sse8387I 3525 CCTGCAGG CCTGCA^GG
StuI 1907 AGGCCT AGG^CCT
XmaCI 3038 CCCGGG C^CCGGG
XmaI 3038 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AatII 683 GACGTC GACGT^C
2632 GACGTC
AflIII 2831 ACACGT A^C[GA][CT]GT
3224 ACATGT
AhdI 2557 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3445 GACAGCTCGTC
Asp700I 2829 GAACACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3330 GAATAACTTC
AspEI 2557 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3445 GACAGCTCGTC
BalI 2379 TGGCCA TGG^CCA
3350 TGGCCA
BanI 930 GGCGCC G^G[CT][GA]CC
3375 GGCGCC
BbeI 930 GGCGCC GGCGC^C
3375 GGCGCC
BglI 2036 GCCGGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3466 GCCTACCGGGC
BsaI 299 GGTCTC GGTCTC(1/5)
1316 GGTCTC
BsmI 872 GCATTC GAATGC(1/-1)
2713 GCATTC
BsrDI 905 CATTGC GCAATG(2/0)
2933 GCAATG
Eam1105I 2557 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3445 GACAGCTCGTC
EcoRI 482 GAATTC G^AATTC
1222 GAATTC
EcoT14I 242 CCTTGG C^C[AT][AT]GG
2210 CCATGG
HaeII 930 GGCGCC [GA]GCGC^[CT]
3375 GGCGCC
KasI 930 GGCGCC G^GCGCC
3375 GGCGCC
MluNI 2379 TGGCCA TGG^CCA
3350 TGGCCA
MscI 2379 TGGCCA TGG^CCA
3350 TGGCCA
MseI 371 TTAA T^TAA
3743 TTAA
NarI 930 GGCGCC GG^CGCC
3375 GGCGCC
PaeR7I 2319 CTCGAG C^TCGAG
2408 CTCGAG
SapI 3183 GAAGAGC GCTCTTC(1/4)
3123 GCTCTTC
SfoI 930 GGCGCC GGC^GCC
3375 GGCGCC
StyI 242 CCTTGG C^C[AT][AT]GG
2210 CCATGG
Tru9I 371 TTAA T^TAA
3743 TTAA
XhoI 2319 CTCGAG C^TCGAG
2408 CTCGAG
XmnI 2829 GAACACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3330 GAATAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanII BbsI BbvCI
BbvI BcnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII