Restriction map for
pFN21AB9339
(Linear) (Six-base) MAPSORT of: KIBB9339.seq Check: 9145 from: 1 to: 3792
KIBB9339.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1419 2178 3792
Size: 1419 759 1614
Fragments arranged by size:
1614 1419 759
AatII G_ACGT'C
Cuts at: 0 687 2636 3792
Size: 687 1949 1156
Fragments arranged by size:
1949 1156 687
AccI GT'mk_AC
Cuts at: 0 1817 3792
Size: 1817 1975
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1809 1821 2917 3395 3678 3792
Size: 1809 12 1096 478 283 114
Fragments arranged by size:
1809 1096 478 283 114 12
AflIII A'CryG_T
Cuts at: 0 2831 3224 3792
Size: 2831 393 568
Fragments arranged by size:
2831 568 393
AgeI A'CCGG_T
Cuts at: 0 1651 3792
Size: 1651 2141
AhdI GACnn_n'nnGTC
Cuts at: 0 2562 3450 3792
Size: 2562 888 342
Fragments arranged by size:
2562 888 342
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2903 2935 3792
Size: 2903 32 857
Fragments arranged by size:
2903 857 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1612 1722 1863 3030 3534 3792
Size: 1612 110 141 1167 504 258
Fragments arranged by size:
1612 1167 504 258 141 110
ApaI G_GGCC'C
Cuts at: 0 2508 3792
Size: 2508 1284
ApoI r'AATT_y
Cuts at: 0 109 268 482 1222 1618 2785 3792
Size: 109 159 214 740 396 1167 1007
Fragments arranged by size:
1167 1007 740 396 214 159 109
AvaI C'yCGr_G
Cuts at: 0 944 1420 1978 2319 2408 3038 3792
Size: 944 476 558 341 89 630 754
Fragments arranged by size:
944 754 630 558 476 341 89
BanI G'GyrC_C
Cuts at: 0 930 3375 3792
Size: 930 2445 417
Fragments arranged by size:
2445 930 417
BanII G_rGCy'C
Cuts at: 0 703 752 818 2191 2508 2756 2867 3125
Size: 703 49 66 1373 317 248 111 258
Cuts at: 3125 3190 3792
Size: 65 602
Fragments arranged by size:
1373 703 602 317 258 248 111 66
65 49
BbeI G_GCGC'C
Cuts at: 0 934 3379 3792
Size: 934 2445 413
Fragments arranged by size:
2445 934 413
BbsI GAAGACnn'nnnn_
Cuts at: 0 596 2910 3792
Size: 596 2314 882
Fragments arranged by size:
2314 882 596
BbvCI CC'TCA_GC
Cuts at: 0 161 1262 1605 2454 3710 3792
Size: 161 1101 343 849 1256 82
Fragments arranged by size:
1256 1101 849 343 161 82
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3423 3457 3792
Size: 3423 34 335
Fragments arranged by size:
3423 335 34
BglI GCCn_nnn'nGGC
Cuts at: 0 2042 3472 3792
Size: 2042 1430 320
Fragments arranged by size:
2042 1430 320
BlpI GC'TnA_GC
Cuts at: 0 495 1120 2944 3119 3792
Size: 495 625 1824 175 673
Fragments arranged by size:
1824 673 625 495 175
Bme1580I G_kGCm'C
Cuts at: 0 360 2060 2508 3792
Size: 360 1700 448 1284
Fragments arranged by size:
1700 1284 448 360
BmrI ACTGGGnnnn_n'
Cuts at: 0 707 803 3195 3243 3792
Size: 707 96 2392 48 549
Fragments arranged by size:
2392 707 549 96 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1749 2805 3792
Size: 1749 1056 987
Fragments arranged by size:
1749 1056 987
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 349 433 466 972 1213 1744 2548
Size: 271 78 84 33 506 241 531 804
Cuts at: 2548 3003 3412 3792
Size: 455 409 380
Fragments arranged by size:
804 531 506 455 409 380 271 241
84 78 33
Bpu10I CC'TnA_GC
Cuts at: 0 161 319 1262 1282 1605 1997 2454 3710
Size: 161 158 943 20 323 392 457 1256
Cuts at: 3710 3792
Size: 82
Fragments arranged by size:
1256 943 457 392 323 161 158 82
20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2590 3792
Size: 2590 1202
BsaI GGTCTCn'nnnn_
Cuts at: 0 305 1322 3792
Size: 305 1017 2470
Fragments arranged by size:
2470 1017 305
BsaHI Gr'CG_yC
Cuts at: 0 684 931 1765 2468 2633 3376 3792
Size: 684 247 834 703 165 743 416
Fragments arranged by size:
834 743 703 684 416 247 165
BsaWI w'CCGG_w
Cuts at: 0 1651 2963 3239 3792
Size: 1651 1312 276 553
Fragments arranged by size:
1651 1312 553 276
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1300 1330 1400 1430 3325 3355 3792
Size: 1300 30 70 30 1895 30 437
Fragments arranged by size:
1895 1300 437 70 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 346 808 851 1664 1795 1923 2136 2407
Size: 346 462 43 813 131 128 213 271
Cuts at: 2407 2426 2560 2764 3193 3727 3792
Size: 19 134 204 429 534 65
Fragments arranged by size:
813 534 462 429 346 271 213 204
134 131 128 65 43 19
BseYI C'CCAG_C
Cuts at: 0 741 1364 2227 2425 3792
Size: 741 623 863 198 1367
Fragments arranged by size:
1367 863 741 623 198
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 1876 3417 3792
Size: 487 1389 1541 375
Fragments arranged by size:
1541 1389 487 375
BsiHKAI G_wGCw'C
Cuts at: 0 752 818 1174 1537 1781 2191 2756 2867
Size: 752 66 356 363 244 410 565 111
Cuts at: 2867 2963 3125 3215 3792
Size: 96 162 90 577
Fragments arranged by size:
752 577 565 410 363 356 244 162
111 96 90 66
BsmI GAATG_Cn'
Cuts at: 0 872 2713 3792
Size: 872 1841 1079
Fragments arranged by size:
1841 1079 872
BsmBI CGTCTCn'nnnn_
Cuts at: 0 695 2022 2483 3792
Size: 695 1327 461 1309
Fragments arranged by size:
1327 1309 695 461
Bsp1286I G_dGCh'C
Cuts at: 0 360 703 752 818 1174 1537 1781 2060
Size: 360 343 49 66 356 363 244 279
Cuts at: 2060 2191 2508 2756 2867 2963 3125 3190 3215
Size: 131 317 248 111 96 162 65 25
Cuts at: 3215 3792
Size: 577
Fragments arranged by size:
577 363 360 356 343 317 279 248
244 162 131 111 96 66 65 49
25
BspEI T'CCGG_A
Cuts at: 0 2963 3792
Size: 2963 829
BspHI T'CATG_A
Cuts at: 0 3308 3792
Size: 3308 484
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1419 1535 2178 3792
Size: 1419 116 643 1614
Fragments arranged by size:
1614 1419 643 116
BsrBI CCG'CTC
Cuts at: 0 5 1488 1996 3792
Size: 5 1483 508 1796
Fragments arranged by size:
1796 1483 508 5
BsrDI GCAATG_nn'
Cuts at: 0 904 2940 3792
Size: 904 2036 852
Fragments arranged by size:
2036 904 852
BsrFI r'CCGG_y
Cuts at: 0 1651 2036 2215 2421 3792
Size: 1651 385 179 206 1371
Fragments arranged by size:
1651 1371 385 206 179
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3629 3792
Size: 3629 163
BstEII G'GTnAC_C
Cuts at: 0 1654 3792
Size: 1654 2138
BstXI CCAn_nnnn'nTGG
Cuts at: 0 288 1072 2235 3792
Size: 288 784 1163 1557
Fragments arranged by size:
1557 1163 784 288
BstYI r'GATC_y
Cuts at: 0 246 776 2448 2644 2891 3792
Size: 246 530 1672 196 247 901
Fragments arranged by size:
1672 901 530 247 246 196
BstZ17I GTA'TAC
Cuts at: 0 1818 3792
Size: 1818 1974
Bsu36I CC'TnA_GG
Cuts at: 0 3146 3792
Size: 3146 646
BtgI C'CryG_G
Cuts at: 0 830 2210 2234 2637 3792
Size: 830 1380 24 403 1155
Fragments arranged by size:
1380 1155 830 403 24
BtsI GCAGTG_nn'
Cuts at: 0 1174 1995 2502 3792
Size: 1174 821 507 1290
Fragments arranged by size:
1290 1174 821 507
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 887 922 3792
Size: 887 35 2870
Fragments arranged by size:
2870 887 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 798 3792
Size: 798 2994
EaeI y'GGCC_r
Cuts at: 0 1356 2213 2379 3350 3422 3792
Size: 1356 857 166 971 72 370
Fragments arranged by size:
1356 971 857 370 166 72
EarI CTCTTCn'nnn_
Cuts at: 0 87 510 833 2154 2882 3130 3178 3776
Size: 87 423 323 1321 728 248 48 598
Cuts at: 3776 3792
Size: 16
Fragments arranged by size:
1321 728 598 423 323 248 87 48
16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1471 2114 2126 2911 3792
Size: 1471 643 12 785 881
Fragments arranged by size:
1471 881 785 643 12
EcoICRI GAG'CTC
Cuts at: 0 750 816 2189 2754 2865 3123 3792
Size: 750 66 1373 565 111 258 669
Fragments arranged by size:
1373 750 669 565 258 111 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 349 433 466 972 1213 1744 1809
Size: 271 78 84 33 506 241 531 65
Cuts at: 1809 1821 2548 2917 3003 3395 3412 3678 3792
Size: 12 727 369 86 392 17 266 114
Fragments arranged by size:
727 531 506 392 369 271 266 241
114 86 84 78 65 33 17 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1810 2980 3484 3709 3792
Size: 1810 1170 504 225 83
Fragments arranged by size:
1810 1170 504 225 83
EcoO109I rG'GnC_Cy
Cuts at: 0 1524 2001 2081 2504 2505 2732 3062 3417
Size: 1524 477 80 423 1 227 330 355
Cuts at: 3417 3531 3717 3792
Size: 114 186 75
Fragments arranged by size:
1524 477 423 355 330 227 186 114
80 75 1
EcoRI G'AATT_C
Cuts at: 0 482 1222 3792
Size: 482 740 2570
Fragments arranged by size:
2570 740 482
HaeII r_GCGC'y
Cuts at: 0 934 3379 3792
Size: 934 2445 413
Fragments arranged by size:
2445 934 413
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1126 1158 1300 1332 1498 1530 2548 2580
Size: 1126 32 142 32 166 32 1018 32
Cuts at: 2580 3436 3468 3792
Size: 856 32 324
Fragments arranged by size:
1126 1018 856 324 166 142 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 809 1029 1649 2882 3792
Size: 809 220 620 1233 910
Fragments arranged by size:
1233 910 809 620 220
HindIII A'AGCT_T
Cuts at: 0 2114 3792
Size: 2114 1678
KasI G'GCGC_C
Cuts at: 0 930 3375 3792
Size: 930 2445 417
Fragments arranged by size:
2445 930 417
MfeI C'AATT_G
Cuts at: 0 395 3792
Size: 395 3397
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 981 2522 3792
Size: 718 263 1541 1270
Fragments arranged by size:
1541 1270 718 263
MscI TGG'CCA
Cuts at: 0 2381 3352 3792
Size: 2381 971 440
Fragments arranged by size:
2381 971 440
MslI CAynn'nnrTG
Cuts at: 0 877 1070 3083 3250 3792
Size: 877 193 2013 167 542
Fragments arranged by size:
2013 877 542 193 167
MspA1I CmG'CkG
Cuts at: 0 160 1140 1344 1896 1902 2458 2465 2639
Size: 160 980 204 552 6 556 7 174
Cuts at: 2639 3792
Size: 1153
Fragments arranged by size:
1153 980 556 552 204 174 160 7
6
NaeI GCC'GGC
Cuts at: 0 2038 3792
Size: 2038 1754
NarI GG'CG_CC
Cuts at: 0 931 3376 3792
Size: 931 2445 416
Fragments arranged by size:
2445 931 416
NcoI C'CATG_G
Cuts at: 0 2210 3792
Size: 2210 1582
NdeI CA'TA_TG
Cuts at: 0 1071 3792
Size: 1071 2721
NgoMIV G'CCGG_C
Cuts at: 0 2036 3792
Size: 2036 1756
NspI r_CATG'y
Cuts at: 0 1704 1716 3228 3627 3657 3792
Size: 1704 12 1512 399 30 135
Fragments arranged by size:
1704 1512 399 135 30 12
PasI CC'CwG_GG
Cuts at: 0 1387 2473 3792
Size: 1387 1086 1319
Fragments arranged by size:
1387 1319 1086
PciI A'CATG_T
Cuts at: 0 3224 3792
Size: 3224 568
PflMI CCAn_nnn'nTGG
Cuts at: 0 1327 2425 3030 3596 3792
Size: 1327 1098 605 566 196
Fragments arranged by size:
1327 1098 605 566 196
PfoI T'CCnGG_A
Cuts at: 0 1174 2975 3792
Size: 1174 1801 817
Fragments arranged by size:
1801 1174 817
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3272 3304 3792
Size: 3272 32 488
Fragments arranged by size:
3272 488 32
PpuMI rG'GwC_Cy
Cuts at: 0 1524 2081 2732 3062 3531 3792
Size: 1524 557 651 330 469 261
Fragments arranged by size:
1524 651 557 469 330 261
PshAI GACnn'nnGTC
Cuts at: 0 687 3792
Size: 687 3105
PsiI TTA'TAA
Cuts at: 0 3676 3792
Size: 3676 116
PspOMI G'GGCC_C
Cuts at: 0 2504 3792
Size: 2504 1288
PspXI vC'TCGA_Gb
Cuts at: 0 2319 3792
Size: 2319 1473
PstI C_TGCA'G
Cuts at: 0 1105 1474 1532 1907 2771 3530 3792
Size: 1105 369 58 375 864 759 262
Fragments arranged by size:
1105 864 759 375 369 262 58
PvuII CAG'CTG
Cuts at: 0 160 1140 1344 1902 3792
Size: 160 980 204 558 1890
Fragments arranged by size:
1890 980 558 204 160
SacI G_AGCT'C
Cuts at: 0 752 818 2191 2756 2867 3125 3792
Size: 752 66 1373 565 111 258 667
Fragments arranged by size:
1373 752 667 565 258 111 66
SacII CC_GC'GG
Cuts at: 0 2640 3792
Size: 2640 1152
SapI GCTCTTCn'nnn_
Cuts at: 0 3130 3178 3792
Size: 3130 48 614
Fragments arranged by size:
3130 614 48
SbfI CC_TGCA'GG
Cuts at: 0 3530 3792
Size: 3530 262
ScaI AGT'ACT
Cuts at: 0 3430 3792
Size: 3430 362
SfcI C'TryA_G
Cuts at: 0 513 1101 1470 1528 1903 2727 2767 3526
Size: 513 588 369 58 375 824 40 759
Cuts at: 3526 3792
Size: 266
Fragments arranged by size:
824 759 588 513 375 369 266 58
40
SfoI GGC'GCC
Cuts at: 0 932 3377 3792
Size: 932 2445 415
Fragments arranged by size:
2445 932 415
SmaI CCC'GGG
Cuts at: 0 3040 3792
Size: 3040 752
SmlI C'TyrA_G
Cuts at: 0 2319 2408 2605 3792
Size: 2319 89 197 1187
Fragments arranged by size:
2319 1187 197 89
SphI G_CATG'C
Cuts at: 0 3627 3792
Size: 3627 165
StuI AGG'CCT
Cuts at: 0 1909 3792
Size: 1909 1883
StyI C'CwwG_G
Cuts at: 0 242 2210 3792
Size: 242 1968 1582
Fragments arranged by size:
1968 1582 242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 135 927 1615 3792
Size: 135 792 688 2177
Fragments arranged by size:
2177 792 688 135
TatI w'GTAC_w
Cuts at: 0 2702 2771 3428 3792
Size: 2702 69 657 364
Fragments arranged by size:
2702 657 364 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1400 1432 2101 2133 3484 3516 3792
Size: 1400 32 669 32 1351 32 276
Fragments arranged by size:
1400 1351 669 276 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 99 285 441 459 1072 1348 3027 3387
Size: 99 186 156 18 613 276 1679 360
Cuts at: 3387 3792
Size: 405
Fragments arranged by size:
1679 613 405 360 276 186 156 99
18
XhoI C'TCGA_G
Cuts at: 0 2319 2408 3792
Size: 2319 89 1384
Fragments arranged by size:
2319 1384 89
XmaI C'CCGG_G
Cuts at: 0 3038 3792
Size: 3038 754
XmnI GAAnn'nnTTC
Cuts at: 0 2833 3334 3792
Size: 2833 501 458
Fragments arranged by size:
2833 501 458
ZraI GAC'GTC
Cuts at: 0 685 2634 3792
Size: 685 1949 1158
Fragments arranged by size:
1949 1158 685
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AgeI AhdI AloI
AlwNI ApaI ApoI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BglI BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BstAPI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtsI CspCI DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HincII
HindIII KasI MfeI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PasI PciI PflMI
PfoI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuII SacI SacII SapI SbfI ScaI SfcI SfoI
SmaI SmlI SphI StuI StyI TaqII TatI TstI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AleI AlfI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI
BglII BmgBI BmtI BsaAI BsaBI BsiEI BsiWI BsrGI
BssHII BssSI BstBI BtgZI ClaI DraI DraIII EagI
EcoRV FalI FseI FspI FspAI HpaI KpnI MluI
NheI NotI NruI NsiI PacI PmeI PmlI PsrI
PvuI RsrII SalI SanDI SexAI SfiI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI Tth111I XbaI