(Circular) (Six-base) MAPSORT of: pFN21AB9239.seq Check: 5400 from: 1 to: 8995 pFN21AB9239 8995 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2285 2285 Size: 8995 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2271 5313 5874 6805 Size: 53 83 186 936 735 3042 561 931 Cuts at: 6805 278 Size: 2468 Fragments arranged by size: 3042 2468 936 931 735 561 186 83 53 AccI GT'mk_AC Cuts at: 1937 3252 5874 6338 1937 Size: 1315 2622 464 4594 Fragments arranged by size: 4594 2622 1315 464 Acc65I G'GTAC_C Cuts at: 6316 6316 Size: 8995 AclI AA'CG_TT Cuts at: 1563 2678 7122 7495 1563 Size: 1115 4444 373 3063 Fragments arranged by size: 4444 3063 1115 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2228 2361 2710 4604 4652 5813 5824 6203 Size: 596 133 349 1894 48 1161 11 379 Cuts at: 6203 7058 8442 8710 1632 Size: 855 1384 268 1917 Fragments arranged by size: 1917 1894 1384 1161 855 596 379 349 268 133 48 11 AflII C'TTAA_G Cuts at: 820 1017 2423 820 Size: 197 1406 7392 Fragments arranged by size: 7392 1406 197 AflIII A'CryG_T Cuts at: 1203 7894 8871 1203 Size: 6691 977 1327 Fragments arranged by size: 6691 1327 977 AgeI A'CCGG_T Cuts at: 7826 7826 Size: 8995 AhdI GACnn_n'nnGTC Cuts at: 2771 7724 2771 Size: 4953 4042 AleI CACnn'nnGTG Cuts at: 1166 4899 1166 Size: 3733 5262 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3474 1184 Size: 2290 6705 AlwNI CAG_nnn'CTG Cuts at: 3394 3560 4054 5218 5728 5791 6738 8310 3394 Size: 166 494 1164 510 63 947 1572 4079 Fragments arranged by size: 4079 1572 1164 947 510 494 166 63 ApaLI G'TGCA_C Cuts at: 7052 8208 7052 Size: 1156 7839 ApoI r'AATT_y Cuts at: 1493 2129 2357 2370 2522 2708 2888 3290 6304 Size: 636 228 13 152 186 180 402 3014 Cuts at: 6304 6530 6682 8791 1493 Size: 226 152 2109 1697 Fragments arranged by size: 3014 2109 1697 636 402 228 226 186 180 152 152 13 AseI AT'TA_AT Cuts at: 160 1032 7549 160 Size: 872 6517 1606 Fragments arranged by size: 6517 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8995 AvaI C'yCGr_G Cuts at: 1870 1943 3211 4090 5280 6241 6291 6320 1870 Size: 73 1268 879 1190 961 50 29 4545 Fragments arranged by size: 4545 1268 1190 961 879 73 50 29 BamHI G'GATC_C Cuts at: 2779 4094 5469 6325 6692 2779 Size: 1315 1375 856 367 5082 Fragments arranged by size: 5082 1375 1315 856 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5221 5985 6125 6257 6316 Size: 325 217 627 3434 764 140 132 59 Cuts at: 6316 7771 618 Size: 1455 1842 Fragments arranged by size: 3434 1842 1455 764 627 325 217 140 132 59 BanII G_rGCy'C Cuts at: 1391 3101 4539 5694 5720 5748 6052 6314 1391 Size: 1710 1438 1155 26 28 304 262 4072 Fragments arranged by size: 4072 1710 1438 1155 304 262 28 26 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2770 3030 4167 928 Size: 971 871 260 1137 5756 Fragments arranged by size: 5756 1137 971 871 260 BbvCI CC'TCA_GC Cuts at: 3695 4139 3695 Size: 444 8551 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3599 3633 4279 4313 7186 7220 1903 Size: 34 1662 34 646 34 2873 34 3678 Fragments arranged by size: 3678 2873 1662 646 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2701 4000 6298 6887 8097 2701 Size: 1299 2298 589 1210 3599 Fragments arranged by size: 3599 2298 1299 1210 589 BclI T'GATC_A Cuts at: 1548 5322 1548 Size: 3774 5221 BfrBI ATG'CAT Cuts at: 5909 5909 Size: 8995 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7606 136 Size: 107 122 71 631 605 5934 1525 Fragments arranged by size: 5934 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3946 8990 3946 Size: 5044 3951 BlpI GC'TnA_GC Cuts at: 5040 6412 5040 Size: 1372 7623 Bme1580I G_kGCm'C Cuts at: 1790 2743 5226 5403 5785 6128 7056 8212 1790 Size: 953 2483 177 382 343 928 1156 2573 Fragments arranged by size: 2573 2483 1156 953 928 382 343 177 BmgBI CAC'GTC Cuts at: 1318 3277 3382 3877 4503 4987 5098 5236 1318 Size: 1959 105 495 626 484 111 138 5077 Fragments arranged by size: 5077 1959 626 495 484 138 111 105 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3265 4592 6726 Size: 416 53 83 399 24 1848 1327 2134 Cuts at: 6726 7684 442 Size: 958 1753 Fragments arranged by size: 2134 1848 1753 1327 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8995 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3043 3043 Size: 8995 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2099 2529 3357 4171 4346 4719 4951 5627 Size: 860 430 828 814 175 373 232 676 Cuts at: 5627 7655 1239 Size: 2028 2579 Fragments arranged by size: 2579 2028 860 828 814 676 430 373 232 175 Bpu10I CC'TnA_GC Cuts at: 3695 4139 5288 5714 3695 Size: 444 1149 426 6976 Fragments arranged by size: 6976 1149 444 426 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2612 4058 4109 7120 7985 8283 2612 Size: 1446 51 3011 865 298 3324 Fragments arranged by size: 3324 3011 1446 865 298 51 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3989 5153 7658 882 Size: 617 2490 1164 2505 2219 Fragments arranged by size: 2505 2490 2219 1164 617 BsaAI yAC'GTr Cuts at: 493 1204 3355 3976 6786 493 Size: 711 2151 621 2810 2702 Fragments arranged by size: 2810 2702 2151 711 621 BsaBI GATnn'nnATC Cuts at: 2477 5069 8989 2477 Size: 2592 3920 2483 Fragments arranged by size: 3920 2592 2483 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2268 3542 4159 5310 Size: 53 83 186 936 735 1274 617 1151 Cuts at: 5310 5871 6802 7184 7819 275 Size: 561 931 382 635 1451 Fragments arranged by size: 1451 1274 1151 936 931 735 635 617 561 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3882 7428 7826 8100 8247 8658 3882 Size: 3546 398 274 147 411 4219 Fragments arranged by size: 4219 3546 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2381 2411 3894 3924 1178 Size: 30 165 30 978 30 1483 30 6249 Fragments arranged by size: 6249 1483 978 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3683 4156 4294 5033 5255 5375 5915 1186 Size: 2497 473 138 739 222 120 540 4266 Fragments arranged by size: 4266 2497 739 540 473 222 138 120 BseYI C'CCAG_C Cuts at: 4802 5314 5721 6068 6151 8198 8694 4802 Size: 512 407 347 83 2047 496 5103 Fragments arranged by size: 5103 2047 512 496 407 347 83 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2225 2378 3418 3434 3830 5009 5110 Size: 573 489 153 1040 16 396 1179 101 Cuts at: 5110 5159 5324 5671 1163 Size: 49 165 347 4487 Fragments arranged by size: 4487 1179 1040 573 489 396 347 165 153 101 49 16 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4368 4694 6445 7206 8234 1540 Size: 275 186 2367 326 1751 761 1028 2301 Fragments arranged by size: 2367 2301 1751 1028 761 326 275 186 BsiHKAI G_wGCw'C Cuts at: 3975 5739 6119 7056 7141 8212 3975 Size: 1764 380 937 85 1071 4758 Fragments arranged by size: 4758 1764 1071 937 380 85 BsmI GAATG_Cn' Cuts at: 6506 6599 6506 Size: 93 8902 BsmBI CGTCTCn'nnnn_ Cuts at: 3533 5603 3533 Size: 2070 6925 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2743 3101 3975 4539 5226 5403 5694 Size: 399 953 358 874 564 687 177 291 Cuts at: 5694 5720 5739 5748 5785 6052 6119 6128 6314 Size: 26 19 9 37 267 67 9 186 Cuts at: 6314 7056 7141 8212 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 953 874 742 687 564 399 358 291 267 186 177 85 67 37 26 19 9 9 BspEI T'CCGG_A Cuts at: 8658 8658 Size: 8995 BspHI T'CATG_A Cuts at: 4677 5751 6884 8614 4677 Size: 1074 1133 1730 5058 Fragments arranged by size: 5058 1730 1133 1074 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2285 2400 3971 4062 4182 5346 6350 844 Size: 1441 115 1571 91 120 1164 1004 3489 Fragments arranged by size: 3489 1571 1441 1164 1004 120 115 91 BsrBI CCG'CTC Cuts at: 1394 1823 2191 5346 6882 1394 Size: 429 368 3155 1536 3507 Fragments arranged by size: 3507 3155 1536 429 368 BsrDI GCAATG_nn' Cuts at: 65 1248 3156 3639 5034 6226 7490 7664 65 Size: 1183 1908 483 1395 1192 1264 174 1396 Fragments arranged by size: 1908 1396 1395 1264 1192 1183 483 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2048 2108 2160 3503 4693 5463 7639 Size: 110 238 60 52 1343 1190 770 2176 Cuts at: 7639 7826 1700 Size: 187 2869 Fragments arranged by size: 2869 2176 1343 1190 770 238 187 110 60 52 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8995 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8995 BssSI C'ACGA_G Cuts at: 2243 3968 6020 7055 8067 2243 Size: 1725 2052 1035 1012 3171 Fragments arranged by size: 3171 2052 1725 1035 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 5853 794 Size: 5059 3936 BstBI TT'CG_AA Cuts at: 4000 4837 7799 4000 Size: 837 2962 5196 Fragments arranged by size: 5196 2962 837 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8995 BstXI CCAn_nnnn'nTGG Cuts at: 5818 5818 Size: 8995 BstYI r'GATC_y Cuts at: 1972 2214 2779 3946 4094 4545 5253 5469 6325 Size: 242 565 1167 148 451 708 216 856 Cuts at: 6325 6692 7077 7094 8546 8632 8644 8990 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1167 856 708 565 451 385 367 346 242 216 148 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5027 5027 Size: 8995 BtgI C'CryG_G Cuts at: 513 1065 3924 5154 5165 5199 5265 5301 5403 Size: 552 2859 1230 11 34 66 36 102 Cuts at: 5403 5539 5572 5599 5765 5817 513 Size: 136 33 27 166 52 3691 Fragments arranged by size: 3691 2859 1230 552 166 136 102 66 52 36 34 33 27 11 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3018 487 Size: 683 56 1792 6464 Fragments arranged by size: 6464 1792 683 56 BtsI GCAGTG_nn' Cuts at: 2071 4357 6511 7305 7325 2071 Size: 2286 2154 794 20 3741 Fragments arranged by size: 3741 2286 2154 794 20 ClaI AT'CG_AT Cuts at: 1554 3219 1554 Size: 1665 7330 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8960 DraI TTT'AAA Cuts at: 2527 6299 2527 Size: 3772 5223 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8002 809 Size: 507 6686 1802 Fragments arranged by size: 6686 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3271 3370 3483 Size: 54 1425 50 275 18 1441 99 113 Cuts at: 3483 3594 3855 4084 4365 4659 5010 5532 5575 Size: 111 261 229 281 294 351 522 43 Cuts at: 5575 5673 6223 6442 7331 8960 8 Size: 98 550 219 889 1629 43 Fragments arranged by size: 1629 1441 1425 889 550 522 351 294 281 275 261 229 219 113 111 99 98 54 50 43 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4365 6442 1537 Size: 275 2553 2077 4090 Fragments arranged by size: 4090 2553 2077 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2337 2783 4028 4527 6925 1353 Size: 984 446 1245 499 2398 3423 Fragments arranged by size: 3423 2398 1245 984 499 446 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5495 7582 7956 8102 387 Size: 1064 138 3906 2087 374 146 1280 Fragments arranged by size: 3906 2087 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2099 2228 2361 2529 2710 3357 4171 Size: 393 467 129 133 168 181 647 814 Cuts at: 4171 4346 4604 4652 4719 4951 5627 5813 5824 Size: 175 258 48 67 232 676 186 11 Cuts at: 5824 6203 7058 7655 8442 8710 1239 Size: 379 855 597 787 268 1524 Fragments arranged by size: 1524 855 814 787 676 647 597 467 393 379 268 258 232 186 181 175 168 133 129 67 48 11 EcoNI CCTnn'n_nnAGG Cuts at: 2243 3562 5975 2243 Size: 1319 2413 5263 Fragments arranged by size: 5263 2413 1319 EcoO109I rG'GnC_Cy Cuts at: 2643 5023 5284 2643 Size: 2380 261 6354 Fragments arranged by size: 6354 2380 261 EcoRI G'AATT_C Cuts at: 6304 6682 6304 Size: 378 8617 EcoRV GAT'ATC Cuts at: 2940 2940 Size: 8995 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5854 5854 Size: 8995 FspI TGC'GCA Cuts at: 5393 7501 5393 Size: 2108 6887 FspAI rTGC'GCAy Cuts at: 5393 5393 Size: 8995 HaeII r_GCGC'y Cuts at: 1678 3920 5174 5218 8142 1678 Size: 2242 1254 44 2924 2531 Fragments arranged by size: 2924 2531 2242 1254 44 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2757 2789 4011 4043 4361 Size: 32 135 32 1185 32 1222 32 318 Cuts at: 4361 4393 4487 4519 5861 5893 7636 7668 7710 Size: 32 94 32 1342 32 1743 32 42 Cuts at: 7710 7742 1373 Size: 32 2626 Fragments arranged by size: 2626 1743 1342 1222 1185 318 135 94 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2572 2701 3121 5569 6339 6585 Size: 988 281 634 129 420 2448 770 246 Cuts at: 6585 669 Size: 3079 Fragments arranged by size: 3079 2448 988 770 634 420 281 246 129 HindIII A'AGCT_T Cuts at: 748 5836 748 Size: 5088 3907 HpaI GTT'AAC Cuts at: 3121 6585 3121 Size: 3464 5531 KpnI G_GTAC'C Cuts at: 6320 6320 Size: 8995 MfeI C'AATT_G Cuts at: 6594 6594 Size: 8995 MluI A'CGCG_T Cuts at: 8871 8871 Size: 8995 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3132 3846 4063 8109 8293 1305 Size: 1827 714 217 4046 184 2007 Fragments arranged by size: 4046 2007 1827 714 217 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3273 3372 3485 4086 4661 Size: 54 1425 343 1441 99 113 601 575 Cuts at: 4661 5012 5534 5675 6225 10 Size: 351 522 141 550 2780 Fragments arranged by size: 2780 1441 1425 601 575 550 522 351 343 141 113 99 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2226 4899 6131 6953 7312 7471 Size: 648 843 217 2673 1232 822 359 159 Cuts at: 7471 518 Size: 2042 Fragments arranged by size: 2673 2042 1232 843 822 648 359 217 159 MspA1I CmG'CkG Cuts at: 1606 2696 4552 4957 4999 5118 5295 5601 5728 Size: 1090 1856 405 42 119 177 306 127 Cuts at: 5728 6255 6411 7088 8236 8481 1606 Size: 527 156 677 1148 245 2120 Fragments arranged by size: 2120 1856 1148 1090 677 527 405 306 245 177 156 127 119 42 NaeI GCC'GGC Cuts at: 2162 3505 2162 Size: 1343 7652 NcoI C'CATG_G Cuts at: 513 1065 5265 5301 5817 513 Size: 552 4200 36 516 3691 Fragments arranged by size: 4200 3691 552 516 36 NdeI CA'TA_TG Cuts at: 387 387 Size: 8995 NgoMIV G'CCGG_C Cuts at: 2160 3503 2160 Size: 1343 7652 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8995 NotI GC'GGCC_GC Cuts at: 6442 6442 Size: 8995 NsiI A_TGCA'T Cuts at: 5911 5911 Size: 8995 NspI r_CATG'y Cuts at: 1132 1229 3759 3775 6353 7898 1132 Size: 97 2530 16 2578 1545 2229 Fragments arranged by size: 2578 2530 2229 1545 97 16 PasI CC'CwG_GG Cuts at: 1348 5971 1348 Size: 4623 4372 PciI A'CATG_T Cuts at: 7894 7894 Size: 8995 PflMI CCAn_nnn'nTGG Cuts at: 1108 2657 5218 1108 Size: 1549 2561 4885 Fragments arranged by size: 4885 2561 1549 PfoI T'CCnGG_A Cuts at: 3225 3423 4097 4928 5106 3225 Size: 198 674 831 178 7114 Fragments arranged by size: 7114 831 674 198 178 PmeI GTTT'AAAC Cuts at: 6299 6299 Size: 8995 PmlI CAC'GTG Cuts at: 3355 3355 Size: 8995 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7064 7096 8577 8609 7064 Size: 32 1481 32 7450 Fragments arranged by size: 7450 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 5023 5284 5023 Size: 261 8734 PshAI GACnn'nnGTC Cuts at: 1613 2267 1613 Size: 654 8341 PsiI TTA'TAA Cuts at: 6565 6565 Size: 8995 PspXI vC'TCGA_Gb Cuts at: 6241 6241 Size: 8995 PstI C_TGCA'G Cuts at: 830 3087 4059 4971 5253 6347 830 Size: 2257 972 912 282 1094 3478 Fragments arranged by size: 3478 2257 1094 972 912 282 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8995 PvuII CAG'CTG Cuts at: 4552 4957 4999 5728 4552 Size: 405 42 729 7819 Fragments arranged by size: 7819 729 405 42 SacII CC_GC'GG Cuts at: 5602 5602 Size: 8995 SalI G'TCGA_C Cuts at: 1936 6337 1936 Size: 4401 4594 SapI GCTCTTCn'nnn_ Cuts at: 4527 4527 Size: 8995 SbfI CC_TGCA'GG Cuts at: 6347 6347 Size: 8995 ScaI AGT'ACT Cuts at: 7243 8955 7243 Size: 1712 7283 SfcI C'TryA_G Cuts at: 826 1045 3083 3188 4055 4967 5249 6343 7478 Size: 219 2038 105 867 912 282 1094 1135 Cuts at: 7478 8159 8350 826 Size: 681 191 1471 Fragments arranged by size: 2038 1471 1135 1094 912 867 681 282 219 191 105 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8995 SgrAI Cr'CCGG_yG Cuts at: 1700 2048 1700 Size: 348 8647 SmaI CCC'GGG Cuts at: 1872 5282 6293 6322 1872 Size: 3410 1011 29 4545 Fragments arranged by size: 4545 3410 1011 29 SmlI C'TyrA_G Cuts at: 820 1017 1943 2423 2591 3211 4073 4124 6241 Size: 197 926 480 168 620 862 51 2117 Cuts at: 6241 7099 8000 8262 820 Size: 858 901 262 1553 Fragments arranged by size: 2117 1553 926 901 862 858 620 480 262 197 168 51 SnaBI TAC'GTA Cuts at: 493 6786 493 Size: 6293 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8995 SphI G_CATG'C Cuts at: 1132 6353 1132 Size: 5221 3774 SspI AAT'ATT Cuts at: 5 52 3178 6919 5 Size: 47 3126 3741 2081 Fragments arranged by size: 3741 3126 2081 47 StuI AGG'CCT Cuts at: 1517 3081 6009 6233 1517 Size: 1564 2928 224 4279 Fragments arranged by size: 4279 2928 1564 224 StyI C'CwwG_G Cuts at: 513 1065 3700 4383 5265 5301 5817 6054 6434 Size: 552 2635 683 882 36 516 237 380 Cuts at: 6434 513 Size: 3074 Fragments arranged by size: 3074 2635 882 683 552 516 380 237 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4679 4864 5503 7022 7039 7192 7377 Size: 1255 3099 185 639 1519 17 153 185 Cuts at: 7377 7822 325 Size: 445 1498 Fragments arranged by size: 3099 1519 1498 1255 639 445 185 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6030 7241 8953 Size: 274 80 33 51 1443 4053 1211 1712 Cuts at: 8953 96 Size: 138 Fragments arranged by size: 4053 1712 1443 1211 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4079 4111 4443 4475 5633 5665 8955 8987 4079 Size: 32 332 32 1158 32 3290 32 4087 Fragments arranged by size: 4087 3290 1158 332 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2152 3071 3666 3878 1316 Size: 836 919 595 212 6433 Fragments arranged by size: 6433 919 836 595 212 XbaI T'CTAG_A Cuts at: 4528 6331 4528 Size: 1803 7192 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4321 5893 4321 Size: 1572 7423 XhoI C'TCGA_G Cuts at: 1943 3211 6241 1943 Size: 1268 3030 4697 Fragments arranged by size: 4697 3030 1268 XmaI C'CCGG_G Cuts at: 1870 5280 6291 6320 1870 Size: 3410 1011 29 4545 Fragments arranged by size: 4545 3410 1011 29 XmnI GAAnn'nnTTC Cuts at: 5088 7124 5088 Size: 2036 6959 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2269 5311 5872 6803 Size: 53 83 186 936 735 3042 561 931 Cuts at: 6803 276 Size: 2468 Fragments arranged by size: 3042 2468 936 931 735 561 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BstZ17I DraIII EcoICRI FseI KasI NarI NruI PacI PspOMI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI