Restriction map for
pFN21AB9177
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# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AhdI ApoI AscI AseI
AsnI Asp718I AspEI AvrII BbeI
BfrI BglII BlnI BsaBI BseAI
BsiWI Bsp1407I BspDI BspEI BsrDI
BsrGI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI EagI
Eam1105I EclXI Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TfiI XbaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 1479 GTCGAC GT^[AC][GT]AC
AgeI 983 ACCGGT A^CCGGT
Aor51HI 110 AGCGCT AGC^GCT
AspI 388 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 209 TGCGCA TGC^GCA
BcgI 946 CGACTCCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1540 GGATAC GTATCC(6/5)
BclI 2570 TGATCA T^GATCA
BlpI 1560 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1560 GCTGAGC GC^T[ACGT]AGC
BsiEI 198 CGACCG CG[GA][CT]^CG
BsmI 2162 GCATTC GAATGC(1/-1)
BspHI 1610 TCATGA T^CATGA
BspLU11I 3356 ACATGT A^CATGT
BssHII 241 GCGCGC G^CGCGC
BssSI 295 CTCGTG CACGAG(-5/-1)
CelII 1560 GCTGAGC GC^T[ACGT]AGC
DraIII 400 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 123 GACTTGCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 110 AGCGCT AGC^GCT
FbaI 2570 TGATCA T^GATCA
FspI 209 TGCGCA TGC^GCA
HincII 1479 GTCGAC GT[CT]^[GA]AC
HindII 1479 GTCGAC GT[CT]^[GA]AC
HindIII 911 AAGCTT A^AGCTT
NcoI 1469 CCATGG C^CATGG
PaeR7I 2825 CTCGAG C^TCGAG
PflFI 388 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 983 ACCGGT A^CCGGT
RcaI 1610 TCATGA T^CATGA
SacI 3181 GAGCTC GAGCT^C
SalI 1479 GTCGAC G^TCGAC
SapI 474 GAAGAGC GCTCTTC(1/4)
SexAI 257 ACCAGGT A^CC[AT]GGT
SmlI 2825 CTCGAG C^T[CT][GA]AG
Tsp509I 2678 AATT ^AATT
Tth111I 388 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2825 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AocI 1524 CCTGAGG CC^T[ACGT]AGG
2092 CCTGAGG
Asp700I 1128 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2916 GAAGCAGTTC
BalI 83 TGGCCA TGG^CCA
3059 TGGCCA
BamHI 484 GGATCC G^GATCC
1046 GGATCC
BbrPI 437 CACGTG CAC^GTG
1906 CACGTG
BbvCI 2227 GCTGAGG CCTCAGC(-5/-2)
3403 GCTGAGG
BsaWI 983 ACCGGT [AT]^CCGG[AT]
1043 ACCGGA
BsrBI 567 GAGCGG CCGCTC(-3/-3)
1774 GAGCGG
Bsu36I 1524 CCTGAGG CC^T[ACGT]AGG
2092 CCTGAGG
Eco81I 1524 CCTGAGG CC^T[ACGT]AGG
2092 CCTGAGG
MluNI 83 TGGCCA TGG^CCA
3059 TGGCCA
MscI 83 TGGCCA TGG^CCA
3059 TGGCCA
MseI 1516 TTAA T^TAA
2341 TTAA
NaeI 1307 GCCGGC GCC^GGC
1565 GCCGGC
NgoMIV 1307 GCCGGC G^CCGGC
1565 GCCGGC
PflMI 1383 CCAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1469 CCATGGACTGG
PmaCI 437 CACGTG CAC^GTG
1906 CACGTG
PmlI 437 CACGTG CAC^GTG
1906 CACGTG
SmaI 220 CCCGGG CCC^GGG
1714 CCCGGG
Tru9I 1516 TTAA T^TAA
2341 TTAA
Van91I 1383 CCAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1469 CCATGGACTGG
XmaCI 220 CCCGGG C^CCGGG
1714 CCCGGG
XmaI 220 CCCGGG C^CCGGG
1714 CCCGGG
XmnI 1128 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2916 GAAGCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SphI
StuI StyI TaqI TseI Tsp45I
TspRI TthHB8I XcmI XhoII