(Circular) (Six-base) MAPSORT of: pFN21AB9177.seq Check: 7624 from: 1 to: 8119 pFN21AB9177 8119 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2391 4039 4328 2391 Size: 1648 289 6182 Fragments arranged by size: 6182 1648 289 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5929 278 Size: 53 83 186 936 4393 2468 Fragments arranged by size: 4393 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3485 5462 1937 Size: 1548 1977 4594 Fragments arranged by size: 4594 1977 1548 Acc65I G'GTAC_C Cuts at: 5440 5440 Size: 8119 AclI AA'CG_TT Cuts at: 1563 6246 6619 1563 Size: 4683 373 3063 Fragments arranged by size: 4683 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4284 4613 6182 7566 7834 1632 Size: 2652 329 1569 1384 268 1917 Fragments arranged by size: 2652 1917 1569 1384 329 268 AfeI AGC'GCT Cuts at: 2117 2117 Size: 8119 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7922 AflIII A'CryG_T Cuts at: 1203 2443 3910 5361 7018 7995 1203 Size: 1240 1467 1451 1657 977 1327 Fragments arranged by size: 1657 1467 1451 1327 1240 977 AgeI A'CCGG_T Cuts at: 2988 6950 2988 Size: 3962 4157 AhdI GACnn_n'nnGTC Cuts at: 6848 6848 Size: 8119 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8119 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4241 1184 Size: 3057 5062 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2519 2551 3254 3286 3537 3569 3782 3814 4964 Size: 32 703 32 251 32 213 32 1150 Cuts at: 4964 4996 2519 Size: 32 5642 Fragments arranged by size: 5642 1150 703 251 213 32 32 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 4285 4371 4873 4912 5862 7434 4285 Size: 86 502 39 950 1572 4970 Fragments arranged by size: 4970 1572 950 502 86 39 ApaI G_GGCC'C Cuts at: 2180 3893 4241 2180 Size: 1713 348 6058 Fragments arranged by size: 6058 1713 348 ApaLI G'TGCA_C Cuts at: 2360 2447 3029 6176 7332 2360 Size: 87 582 3147 1156 3147 Fragments arranged by size: 3147 3147 1156 582 87 ApoI r'AATT_y Cuts at: 1493 5428 5654 5806 7915 1493 Size: 3935 226 152 2109 1697 Fragments arranged by size: 3935 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6673 160 Size: 872 5641 1606 Fragments arranged by size: 5641 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8119 AvaI C'yCGr_G Cuts at: 1870 1943 2225 3719 4178 4411 4481 4830 5007 Size: 73 282 1494 459 233 70 349 177 Cuts at: 5007 5444 1870 Size: 437 4545 Fragments arranged by size: 4545 1494 459 437 349 282 233 177 73 70 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2191 2224 2191 Size: 33 8086 BamHI G'GATC_C Cuts at: 2489 3051 5449 5816 2489 Size: 562 2398 367 4792 Fragments arranged by size: 4792 2398 562 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2017 2759 3241 3715 3842 Size: 325 217 627 230 742 482 474 127 Cuts at: 3842 5440 6895 618 Size: 1598 1455 1842 Fragments arranged by size: 1842 1598 1455 742 627 482 474 325 230 217 127 BanII G_rGCy'C Cuts at: 1391 2165 2180 2380 3893 4179 4241 4307 4488 Size: 774 15 200 1513 286 62 66 181 Cuts at: 4488 5190 5438 1391 Size: 702 248 4072 Fragments arranged by size: 4072 1513 774 702 286 248 200 181 66 62 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2302 3227 5039 928 Size: 971 403 925 1812 4008 Fragments arranged by size: 4008 1812 971 925 403 BbvCI CC'TCA_GC Cuts at: 4233 5409 4233 Size: 1176 6943 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2940 2974 6310 6344 1903 Size: 34 1003 34 3336 34 3678 Fragments arranged by size: 3678 3336 1003 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3539 6011 7221 3539 Size: 2472 1210 4437 Fragments arranged by size: 4437 2472 1210 BclI T'GATC_A Cuts at: 1548 4575 1548 Size: 3027 5092 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2613 2748 5348 Size: 107 122 71 631 605 941 135 2600 Cuts at: 5348 6730 136 Size: 1382 1525 Fragments arranged by size: 2600 1525 1382 941 631 605 135 122 107 71 BglII A'GATC_T Cuts at: 8114 8114 Size: 8119 BlpI GC'TnA_GC Cuts at: 3566 5536 3566 Size: 1970 6149 Bme1580I G_kGCm'C Cuts at: 1790 2022 2180 2364 2451 2690 2822 2926 3033 Size: 232 158 184 87 239 132 104 107 Cuts at: 3033 3246 3342 3351 3720 3893 4241 6180 7336 Size: 213 96 9 369 173 348 1939 1156 Cuts at: 7336 1790 Size: 2573 Fragments arranged by size: 2573 1939 1156 369 348 239 232 213 184 173 158 132 107 104 96 87 9 BmgBI CAC'GTC Cuts at: 1318 2470 4087 1318 Size: 1152 1617 5350 Fragments arranged by size: 5350 1617 1152 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3498 3921 4235 Size: 416 53 83 399 24 2081 423 314 Cuts at: 4235 4493 5850 6808 442 Size: 258 1357 958 1753 Fragments arranged by size: 2081 1753 1357 958 423 416 399 314 258 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8119 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3811 4648 3811 Size: 837 7282 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2213 2394 2914 2939 3104 3921 4207 6779 Size: 974 181 520 25 165 817 286 2572 Cuts at: 6779 1239 Size: 2579 Fragments arranged by size: 2579 2572 974 817 520 286 181 165 25 Bpu10I CC'TnA_GC Cuts at: 3633 4233 4441 4490 4856 5409 3633 Size: 600 208 49 366 553 6343 Fragments arranged by size: 6343 600 553 366 208 49 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6244 7109 7407 6244 Size: 865 298 6956 Fragments arranged by size: 6956 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3403 3415 4062 4415 6782 882 Size: 617 1904 12 647 353 2367 2219 Fragments arranged by size: 2367 2219 1904 647 617 353 12 BsaAI yAC'GTr Cuts at: 493 1204 2359 2444 3913 5910 493 Size: 711 1155 85 1469 1997 2702 Fragments arranged by size: 2702 1997 1469 1155 711 85 BsaBI GATnn'nnATC Cuts at: 8113 8113 Size: 8119 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2169 2307 5044 5926 Size: 53 83 186 936 636 138 2737 882 Cuts at: 5926 6308 6943 275 Size: 382 635 1451 Fragments arranged by size: 2737 1451 936 882 636 635 382 186 138 83 53 BsaWI w'CCGG_w Cuts at: 2988 3048 6552 6950 7224 7371 7782 2988 Size: 60 3504 398 274 147 411 3325 Fragments arranged by size: 3504 3325 411 398 274 147 60 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3351 3381 4964 4994 5004 Size: 30 165 30 1948 30 1583 30 10 Cuts at: 5004 5034 1178 Size: 30 4263 Fragments arranged by size: 4263 1948 1583 165 30 30 30 30 30 10 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3600 4115 4433 5030 5426 1186 Size: 2414 515 318 597 396 3879 Fragments arranged by size: 3879 2414 597 515 396 318 BseYI C'CCAG_C Cuts at: 2112 2330 2654 2661 4055 4819 7322 7818 2112 Size: 218 324 7 1394 764 2503 496 2413 Fragments arranged by size: 2503 2413 1394 764 496 324 218 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2424 2579 3141 3342 3434 3581 3862 Size: 573 688 155 562 201 92 147 281 Cuts at: 3862 3884 3891 4067 4083 1163 Size: 22 7 176 16 5199 Fragments arranged by size: 5199 688 573 562 281 201 176 155 147 92 22 16 7 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2206 5569 6330 7358 1540 Size: 275 186 205 3363 761 1028 2301 Fragments arranged by size: 3363 2301 1028 761 275 205 186 BsiHKAI G_wGCw'C Cuts at: 2364 2451 3033 3189 5190 6180 6265 7336 2364 Size: 87 582 156 2001 990 85 1071 3147 Fragments arranged by size: 3147 2001 1071 990 582 156 87 85 BsmI GAATG_Cn' Cuts at: 4167 5630 5723 4167 Size: 1463 93 6563 Fragments arranged by size: 6563 1463 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3092 3799 4378 5051 3092 Size: 707 579 673 6160 Fragments arranged by size: 6160 707 673 579 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2165 2180 2364 2380 2451 2690 Size: 399 232 143 15 184 16 71 239 Cuts at: 2690 2822 2926 3033 3189 3246 3342 3351 3720 Size: 132 104 107 156 57 96 9 369 Cuts at: 3720 3893 4179 4241 4307 4488 5190 5438 6180 Size: 173 286 62 66 181 702 248 742 Cuts at: 6180 6265 7336 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 702 399 369 286 248 239 232 184 181 173 156 143 132 107 104 96 85 71 66 62 57 16 15 9 BspEI T'CCGG_A Cuts at: 7782 7782 Size: 8119 BspHI T'CATG_A Cuts at: 3615 6008 7738 3615 Size: 2393 1730 3996 Fragments arranged by size: 3996 2393 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2103 2391 4039 4328 4785 5474 844 Size: 1259 288 1648 289 457 689 3489 Fragments arranged by size: 3489 1648 1259 689 457 289 288 BsrBI CCG'CTC Cuts at: 1394 1823 2574 3781 6006 1394 Size: 429 751 1207 2225 3507 Fragments arranged by size: 3507 2225 1207 751 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6614 6788 65 Size: 1183 5366 174 1396 Fragments arranged by size: 5366 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2988 3312 3456 3570 3684 6763 6950 Size: 110 1178 324 144 114 114 3079 187 Cuts at: 6950 1700 Size: 2869 Fragments arranged by size: 3079 2869 1178 324 187 144 114 114 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8119 BssHII G'CGCG_C Cuts at: 1925 2246 1925 Size: 321 7798 BssSI C'ACGA_G Cuts at: 2300 6179 7191 2300 Size: 3879 1012 3228 Fragments arranged by size: 3879 3228 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3028 4371 4873 794 Size: 2234 1343 502 4040 Fragments arranged by size: 4040 2234 1343 502 BstBI TT'CG_AA Cuts at: 6923 6923 Size: 8119 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8119 BstXI CCAn_nnnn'nTGG Cuts at: 2596 2611 3496 2596 Size: 15 885 7219 Fragments arranged by size: 7219 885 15 BstYI r'GATC_y Cuts at: 1972 2489 2885 3051 3060 3981 5449 5816 6201 Size: 517 396 166 9 921 1468 367 385 Cuts at: 6201 6218 7670 7756 7768 8114 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1468 1452 921 517 396 385 367 346 166 86 17 12 9 Bsu36I CC'TnA_GG Cuts at: 3530 4098 3530 Size: 568 7551 BtgI C'CryG_G Cuts at: 513 1065 2004 3474 3650 4120 513 Size: 552 939 1470 176 470 4512 Fragments arranged by size: 4512 1470 939 552 470 176 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7380 Fragments arranged by size: 7380 683 56 BtsI GCAGTG_nn' Cuts at: 2822 3813 3874 5635 6429 6449 2822 Size: 991 61 1761 794 20 4492 Fragments arranged by size: 4492 1761 991 794 61 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8119 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8084 DraI TTT'AAA Cuts at: 5423 5423 Size: 8119 DraIII CAC_nnn'GTG Cuts at: 2410 2410 Size: 8119 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2134 7126 809 Size: 507 818 4992 1802 Fragments arranged by size: 4992 1802 818 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2088 3301 3572 Size: 54 1425 50 275 18 258 1213 271 Cuts at: 3572 4335 4466 4555 4893 5064 5566 6455 8084 Size: 763 131 89 338 171 502 889 1629 Cuts at: 8084 8 Size: 43 Fragments arranged by size: 1629 1425 1213 889 763 502 338 275 271 258 171 131 89 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5566 1537 Size: 275 3754 4090 Fragments arranged by size: 4090 3754 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2048 2474 4154 4197 4260 6049 1353 Size: 695 426 1680 43 63 1789 3423 Fragments arranged by size: 3423 1789 1680 695 426 63 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3434 6706 7080 7226 387 Size: 1064 138 1845 3272 374 146 1280 Fragments arranged by size: 3272 1845 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5188 5188 Size: 8119 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 2394 2914 2939 3104 3921 4207 Size: 393 581 181 520 25 165 817 286 Cuts at: 4207 4284 4613 6182 6779 7566 7834 1239 Size: 77 329 1569 597 787 268 1524 Fragments arranged by size: 1569 1524 817 787 597 581 520 393 329 286 268 181 165 77 25 EcoO109I rG'GnC_Cy Cuts at: 2176 2842 3594 3602 3964 4237 4270 4446 4531 Size: 666 752 8 362 273 33 176 85 Cuts at: 4531 4719 4804 4939 2176 Size: 188 85 135 5356 Fragments arranged by size: 5356 752 666 362 273 188 176 135 85 85 33 8 EcoRI G'AATT_C Cuts at: 5428 5806 5428 Size: 378 7741 FspI TGC'GCA Cuts at: 2216 6625 2216 Size: 4409 3710 FspAI rTGC'GCAy Cuts at: 2216 2216 Size: 8119 HaeII r_GCGC'y Cuts at: 1678 2119 2622 2662 3319 7266 1678 Size: 441 503 40 657 3947 2531 Fragments arranged by size: 3947 2531 657 503 441 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5029 5061 5099 5131 6760 Size: 32 135 32 3457 32 38 32 1629 Cuts at: 6760 6792 6834 6866 1373 Size: 32 42 32 2626 Fragments arranged by size: 3457 2626 1629 135 42 38 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3486 5463 5709 669 Size: 988 281 1548 1977 246 3079 Fragments arranged by size: 3079 1977 1548 988 281 246 HindIII A'AGCT_T Cuts at: 748 2916 748 Size: 2168 5951 HpaI GTT'AAC Cuts at: 5709 5709 Size: 8119 KpnI G_GTAC'C Cuts at: 5444 5444 Size: 8119 MfeI C'AATT_G Cuts at: 5718 5718 Size: 8119 MluI A'CGCG_T Cuts at: 7995 7995 Size: 8119 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3636 4945 7233 7417 1305 Size: 2331 1309 2288 184 2007 Fragments arranged by size: 2331 2288 2007 1309 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2090 5066 10 Size: 54 1425 343 258 2976 3063 Fragments arranged by size: 3063 2976 1425 343 258 54 MslI CAynn'nnrTG Cuts at: 518 1166 2357 2706 2721 3494 3614 4613 6077 Size: 648 1191 349 15 773 120 999 1464 Cuts at: 6077 6436 6595 518 Size: 359 159 2042 Fragments arranged by size: 2042 1464 1191 999 773 648 359 349 159 120 15 MspA1I CmG'CkG Cuts at: 1606 2429 2766 3125 3230 3406 3565 3856 3988 Size: 823 337 359 105 176 159 291 132 Cuts at: 3988 4262 4285 4870 5408 5535 6212 7360 7605 Size: 274 23 585 538 127 677 1148 245 Cuts at: 7605 1606 Size: 2120 Fragments arranged by size: 2120 1148 823 677 585 538 359 337 291 274 245 176 159 132 127 105 23 NaeI GCC'GGC Cuts at: 3314 3572 3314 Size: 258 7861 NcoI C'CATG_G Cuts at: 513 1065 2004 3474 513 Size: 552 939 1470 5158 Fragments arranged by size: 5158 1470 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8119 NgoMIV G'CCGG_C Cuts at: 3312 3570 3312 Size: 258 7861 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8119 NotI GC'GGCC_GC Cuts at: 5566 5566 Size: 8119 NspI r_CATG'y Cuts at: 1132 1229 3671 4159 4906 5335 5365 5477 7022 Size: 97 2442 488 747 429 30 112 1545 Cuts at: 7022 1132 Size: 2229 Fragments arranged by size: 2442 2229 1545 747 488 429 112 97 30 PasI CC'CwG_GG Cuts at: 1348 2611 4213 5206 1348 Size: 1263 1602 993 4261 Fragments arranged by size: 4261 1602 1263 993 PciI A'CATG_T Cuts at: 5361 7018 5361 Size: 1657 6462 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 3394 3480 1108 Size: 902 1384 86 5747 Fragments arranged by size: 5747 1384 902 86 PmeI GTTT'AAAC Cuts at: 5423 5423 Size: 8119 PmlI CAC'GTG Cuts at: 2444 3913 2444 Size: 1469 6650 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4910 4942 4964 4996 6188 6220 7701 7733 4910 Size: 32 22 32 1192 32 1481 32 5296 Fragments arranged by size: 5296 1481 1192 32 32 32 32 22 PpuMI rG'GwC_Cy Cuts at: 4446 4531 4804 4939 4446 Size: 85 273 135 7626 Fragments arranged by size: 7626 273 135 85 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8119 PsiI TTA'TAA Cuts at: 5689 5689 Size: 8119 PspOMI G'GGCC_C Cuts at: 2176 3889 4237 2176 Size: 1713 348 6058 Fragments arranged by size: 6058 1713 348 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4721 4753 5369 5401 4721 Size: 32 616 32 7439 Fragments arranged by size: 7439 616 32 32 PstI C_TGCA'G Cuts at: 830 2547 2783 3130 4230 4434 4479 5471 830 Size: 1717 236 347 1100 204 45 992 3478 Fragments arranged by size: 3478 1717 1100 992 347 236 204 45 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8119 PvuII CAG'CTG Cuts at: 3125 3230 3565 3856 3988 4262 4285 4870 5408 Size: 105 335 291 132 274 23 585 538 Cuts at: 5408 3125 Size: 5836 Fragments arranged by size: 5836 585 538 335 291 274 132 105 23 SacI G_AGCT'C Cuts at: 5190 5190 Size: 8119 SalI G'TCGA_C Cuts at: 1936 3484 5461 1936 Size: 1548 1977 4594 Fragments arranged by size: 4594 1977 1548 SapI GCTCTTCn'nnn_ Cuts at: 2474 2474 Size: 8119 SbfI CC_TGCA'GG Cuts at: 5471 5471 Size: 8119 ScaI AGT'ACT Cuts at: 6367 8079 6367 Size: 1712 6407 SexAI A'CCwGG_T Cuts at: 2262 2262 Size: 8119 SfcI C'TryA_G Cuts at: 826 1045 2543 2581 2779 3126 4226 4430 4475 Size: 219 1498 38 198 347 1100 204 45 Cuts at: 4475 5083 5318 5467 6602 7283 7474 826 Size: 608 235 149 1135 681 191 1471 Fragments arranged by size: 1498 1471 1135 1100 681 608 347 235 219 204 198 191 149 45 38 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8119 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8119 SmaI CCC'GGG Cuts at: 1872 2227 3721 5446 1872 Size: 355 1494 1725 4545 Fragments arranged by size: 4545 1725 1494 355 SmlI C'TyrA_G Cuts at: 820 1017 1943 4830 6223 7124 7386 820 Size: 197 926 2887 1393 901 262 1553 Fragments arranged by size: 2887 1553 1393 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5910 493 Size: 5417 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8119 SphI G_CATG'C Cuts at: 1132 4159 4906 5335 5477 1132 Size: 3027 747 429 142 3774 Fragments arranged by size: 3774 3027 747 429 142 SrfI GCCC'GGGC Cuts at: 2227 3721 2227 Size: 1494 6625 SspI AAT'ATT Cuts at: 5 52 6043 5 Size: 47 5991 2081 Fragments arranged by size: 5991 2081 47 StuI AGG'CCT Cuts at: 1517 2777 4096 5351 1517 Size: 1260 1319 1255 4285 Fragments arranged by size: 4285 1319 1260 1255 StyI C'CwwG_G Cuts at: 513 1065 2004 2679 3198 3474 3711 5149 5558 Size: 552 939 675 519 276 237 1438 409 Cuts at: 5558 513 Size: 3074 Fragments arranged by size: 3074 1438 939 675 552 519 409 276 237 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3006 3860 6146 6163 6316 6501 6946 Size: 1255 1426 854 2286 17 153 185 445 Cuts at: 6946 325 Size: 1498 Fragments arranged by size: 2286 1498 1426 1255 854 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2431 6365 8077 Size: 274 80 33 51 1443 454 3934 1712 Cuts at: 8077 96 Size: 138 Fragments arranged by size: 3934 1712 1443 454 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3103 3135 3351 3383 3613 3645 4661 4693 8079 Size: 32 216 32 230 32 1016 32 3386 Cuts at: 8079 8111 3103 Size: 32 3111 Fragments arranged by size: 3386 3111 1016 230 216 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2396 1316 Size: 1080 7039 XbaI T'CTAG_A Cuts at: 5455 5455 Size: 8119 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2044 2969 3205 4036 4506 2044 Size: 925 236 831 470 5657 Fragments arranged by size: 5657 925 831 470 236 XhoI C'TCGA_G Cuts at: 1943 4830 1943 Size: 2887 5232 XmaI C'CCGG_G Cuts at: 1870 2225 3719 5444 1870 Size: 355 1494 1725 4545 Fragments arranged by size: 4545 1725 1494 355 XmnI GAAnn'nnTTC Cuts at: 3137 4925 6248 3137 Size: 1788 1323 5008 Fragments arranged by size: 5008 1788 1323 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5927 276 Size: 53 83 186 936 4393 2468 Fragments arranged by size: 4393 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BbeI BfrBI BsiWI BstZ17I EcoNI EcoRV FalI FseI KasI NarI NruI NsiI PacI PfoI PspXI RsrII SacII SanDI SfiI SfoI SwaI