Restriction map for
pFN21AB9177
(Linear) (Six-base) MAPSORT of: KIBB9177.seq Check: 2505 from: 1 to: 3414
KIBB9177.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 386 2034 2323 3414
Size: 386 1648 289 1091
Fragments arranged by size:
1648 1091 386 289
AccI GT'mk_AC
Cuts at: 0 1480 3414
Size: 1480 1934
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2279 2608 3414
Size: 2279 329 806
Fragments arranged by size:
2279 806 329
AfeI AGC'GCT
Cuts at: 0 112 3414
Size: 112 3302
AflIII A'CryG_T
Cuts at: 0 438 1905 3356 3414
Size: 438 1467 1451 58
Fragments arranged by size:
1467 1451 438 58
AgeI A'CCGG_T
Cuts at: 0 983 3414
Size: 983 2431
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2236 3414
Size: 2236 1178
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 514 546 1249 1281 1532 1564 1777 1809
Size: 514 32 703 32 251 32 213 32
Cuts at: 1809 2959 2991 3414
Size: 1150 32 423
Fragments arranged by size:
1150 703 514 423 251 213 32 32
32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2280 2366 2868 2907 3414
Size: 2280 86 502 39 507
Fragments arranged by size:
2280 507 502 86 39
ApaI G_GGCC'C
Cuts at: 0 175 1888 2236 3414
Size: 175 1713 348 1178
Fragments arranged by size:
1713 1178 348 175
ApaLI G'TGCA_C
Cuts at: 0 355 442 1024 3414
Size: 355 87 582 2390
Fragments arranged by size:
2390 582 355 87
AvaI C'yCGr_G
Cuts at: 0 220 1714 2173 2406 2476 2825 3002 3414
Size: 220 1494 459 233 70 349 177 412
Fragments arranged by size:
1494 459 412 349 233 220 177 70
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 186 219 3414
Size: 186 33 3195
Fragments arranged by size:
3195 186 33
BamHI G'GATC_C
Cuts at: 0 484 1046 3414
Size: 484 562 2368
Fragments arranged by size:
2368 562 484
BanI G'GyrC_C
Cuts at: 0 12 754 1236 1710 1837 3414
Size: 12 742 482 474 127 1577
Fragments arranged by size:
1577 742 482 474 127 12
BanII G_rGCy'C
Cuts at: 0 160 175 375 1888 2174 2236 2302 2483
Size: 160 15 200 1513 286 62 66 181
Cuts at: 2483 3185 3414
Size: 702 229
Fragments arranged by size:
1513 702 286 229 200 181 160 66
62 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 297 1222 3034 3414
Size: 297 925 1812 380
Fragments arranged by size:
1812 925 380 297
BbvCI CC'TCA_GC
Cuts at: 0 2228 3404 3414
Size: 2228 1176 10
Fragments arranged by size:
2228 1176 10
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 935 969 3414
Size: 935 34 2445
Fragments arranged by size:
2445 935 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1534 3414
Size: 1534 1880
BclI T'GATC_A
Cuts at: 0 2570 3414
Size: 2570 844
BglI GCCn_nnn'nGGC
Cuts at: 0 608 743 3343 3414
Size: 608 135 2600 71
Fragments arranged by size:
2600 608 135 71
BlpI GC'TnA_GC
Cuts at: 0 1561 3414
Size: 1561 1853
Bme1580I G_kGCm'C
Cuts at: 0 17 175 359 446 685 817 921 1028
Size: 17 158 184 87 239 132 104 107
Cuts at: 1028 1241 1337 1346 1715 1888 2236 3414
Size: 213 96 9 369 173 348 1178
Fragments arranged by size:
1178 369 348 239 213 184 173 158
132 107 104 96 87 17 9
BmgBI CAC'GTC
Cuts at: 0 465 2082 3414
Size: 465 1617 1332
Fragments arranged by size:
1617 1332 465
BmrI ACTGGGnnnn_n'
Cuts at: 0 1493 1916 2230 2488 3414
Size: 1493 423 314 258 926
Fragments arranged by size:
1493 926 423 314 258
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1806 2643 3414
Size: 1806 837 771
Fragments arranged by size:
1806 837 771
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 389 909 934 1099 1916 2202 3414
Size: 208 181 520 25 165 817 286 1212
Fragments arranged by size:
1212 817 520 286 208 181 165 25
Bpu10I CC'TnA_GC
Cuts at: 0 1628 2228 2436 2485 2851 3404 3414
Size: 1628 600 208 49 366 553 10
Fragments arranged by size:
1628 600 553 366 208 49 10
BsaI GGTCTCn'nnnn_
Cuts at: 0 1398 1410 2057 2410 3414
Size: 1398 12 647 353 1004
Fragments arranged by size:
1398 1004 647 353 12
BsaAI yAC'GTr
Cuts at: 0 354 439 1908 3414
Size: 354 85 1469 1506
Fragments arranged by size:
1506 1469 354 85
BsaHI Gr'CG_yC
Cuts at: 0 164 302 3039 3414
Size: 164 138 2737 375
Fragments arranged by size:
2737 375 164 138
BsaWI w'CCGG_w
Cuts at: 0 983 1043 3414
Size: 983 60 2371
Fragments arranged by size:
2371 983 60
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1346 1376 2959 2989 2999 3029 3414
Size: 1346 30 1583 30 10 30 385
Fragments arranged by size:
1583 1346 385 30 30 30 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1595 2110 2428 3025 3414
Size: 1595 515 318 597 389
Fragments arranged by size:
1595 597 515 389 318
BseYI C'CCAG_C
Cuts at: 0 107 325 649 656 2050 2814 3414
Size: 107 218 324 7 1394 764 600
Fragments arranged by size:
1394 764 600 324 218 107 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 419 574 1136 1337 1429 1576 1857 1879
Size: 419 155 562 201 92 147 281 22
Cuts at: 1879 1886 2062 2078 3414
Size: 7 176 16 1336
Fragments arranged by size:
1336 562 419 281 201 176 155 147
92 22 16 7
BsiEI CG_ry'CG
Cuts at: 0 201 3414
Size: 201 3213
BsiHKAI G_wGCw'C
Cuts at: 0 359 446 1028 1184 3185 3414
Size: 359 87 582 156 2001 229
Fragments arranged by size:
2001 582 359 229 156 87
BsmI GAATG_Cn'
Cuts at: 0 2162 3414
Size: 2162 1252
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1087 1794 2373 3046 3414
Size: 1087 707 579 673 368
Fragments arranged by size:
1087 707 673 579 368
Bsp1286I G_dGCh'C
Cuts at: 0 17 160 175 359 375 446 685 817
Size: 17 143 15 184 16 71 239 132
Cuts at: 817 921 1028 1184 1241 1337 1346 1715 1888
Size: 104 107 156 57 96 9 369 173
Cuts at: 1888 2174 2236 2302 2483 3185 3414
Size: 286 62 66 181 702 229
Fragments arranged by size:
702 369 286 239 229 184 181 173
156 143 132 107 104 96 71 66
62 57 17 16 15 9
BspHI T'CATG_A
Cuts at: 0 1610 3414
Size: 1610 1804
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 98 386 2034 2323 2780 3414
Size: 98 288 1648 289 457 634
Fragments arranged by size:
1648 634 457 289 288 98
BsrBI CCG'CTC
Cuts at: 0 569 1776 3414
Size: 569 1207 1638
Fragments arranged by size:
1638 1207 569
BsrFI r'CCGG_y
Cuts at: 0 983 1307 1451 1565 1679 3414
Size: 983 324 144 114 114 1735
Fragments arranged by size:
1735 983 324 144 114 114
BssHII G'CGCG_C
Cuts at: 0 241 3414
Size: 241 3173
BssSI C'ACGA_G
Cuts at: 0 295 3414
Size: 295 3119
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1023 2366 2868 3414
Size: 1023 1343 502 546
Fragments arranged by size:
1343 1023 546 502
BstXI CCAn_nnnn'nTGG
Cuts at: 0 591 606 1491 3414
Size: 591 15 885 1923
Fragments arranged by size:
1923 885 591 15
BstYI r'GATC_y
Cuts at: 0 484 880 1046 1055 1976 3414
Size: 484 396 166 9 921 1438
Fragments arranged by size:
1438 921 484 396 166 9
Bsu36I CC'TnA_GG
Cuts at: 0 1525 2093 3414
Size: 1525 568 1321
Fragments arranged by size:
1525 1321 568
BtgI C'CryG_G
Cuts at: 0 1469 1645 2115 3414
Size: 1469 176 470 1299
Fragments arranged by size:
1469 1299 470 176
BtsI GCAGTG_nn'
Cuts at: 0 817 1808 1869 3414
Size: 817 991 61 1545
Fragments arranged by size:
1545 991 817 61
DraIII CAC_nnn'GTG
Cuts at: 0 405 3414
Size: 405 3009
DrdI GACnn_nn'nnGTC
Cuts at: 0 129 3414
Size: 129 3285
EaeI y'GGCC_r
Cuts at: 0 83 1296 1567 2330 2461 2550 2888 3059
Size: 83 1213 271 763 131 89 338 171
Cuts at: 3059 3414
Size: 355
Fragments arranged by size:
1213 763 355 338 271 171 131 89
83
EarI CTCTTCn'nnn_
Cuts at: 0 43 469 2149 2192 2255 3414
Size: 43 426 1680 43 63 1159
Fragments arranged by size:
1680 1159 426 63 43 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1429 3414
Size: 1429 1985
EcoICRI GAG'CTC
Cuts at: 0 3183 3414
Size: 3183 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 389 909 934 1099 1916 2202 2279
Size: 208 181 520 25 165 817 286 77
Cuts at: 2279 2608 3414
Size: 329 806
Fragments arranged by size:
817 806 520 329 286 208 181 165
77 25
EcoO109I rG'GnC_Cy
Cuts at: 0 171 837 1589 1597 1959 2232 2265 2441
Size: 171 666 752 8 362 273 33 176
Cuts at: 2441 2526 2714 2799 2934 3414
Size: 85 188 85 135 480
Fragments arranged by size:
752 666 480 362 273 188 176 171
135 85 85 33 8
FspI TGC'GCA
Cuts at: 0 211 3414
Size: 211 3203
FspAI rTGC'GCAy
Cuts at: 0 211 3414
Size: 211 3203
HaeII r_GCGC'y
Cuts at: 0 114 617 657 1314 3414
Size: 114 503 40 657 2100
Fragments arranged by size:
2100 657 503 114 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3024 3056 3094 3126 3414
Size: 3024 32 38 32 288
Fragments arranged by size:
3024 288 38 32 32
HincII GTy'rAC
Cuts at: 0 1481 3414
Size: 1481 1933
HindIII A'AGCT_T
Cuts at: 0 911 3414
Size: 911 2503
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1631 2940 3414
Size: 1631 1309 474
Fragments arranged by size:
1631 1309 474
MscI TGG'CCA
Cuts at: 0 85 3061 3414
Size: 85 2976 353
Fragments arranged by size:
2976 353 85
MslI CAynn'nnrTG
Cuts at: 0 352 701 716 1489 1609 2608 3414
Size: 352 349 15 773 120 999 806
Fragments arranged by size:
999 806 773 352 349 120 15
MspA1I CmG'CkG
Cuts at: 0 424 761 1120 1225 1401 1560 1851 1983
Size: 424 337 359 105 176 159 291 132
Cuts at: 1983 2257 2280 2865 3403 3414
Size: 274 23 585 538 11
Fragments arranged by size:
585 538 424 359 337 291 274 176
159 132 105 23 11
NaeI GCC'GGC
Cuts at: 0 1309 1567 3414
Size: 1309 258 1847
Fragments arranged by size:
1847 1309 258
NcoI C'CATG_G
Cuts at: 0 1469 3414
Size: 1469 1945
NgoMIV G'CCGG_C
Cuts at: 0 1307 1565 3414
Size: 1307 258 1849
Fragments arranged by size:
1849 1307 258
NspI r_CATG'y
Cuts at: 0 1666 2154 2901 3330 3360 3414
Size: 1666 488 747 429 30 54
Fragments arranged by size:
1666 747 488 429 54 30
PasI CC'CwG_GG
Cuts at: 0 606 2208 3201 3414
Size: 606 1602 993 213
Fragments arranged by size:
1602 993 606 213
PciI A'CATG_T
Cuts at: 0 3356 3414
Size: 3356 58
PflMI CCAn_nnn'nTGG
Cuts at: 0 1389 1475 3414
Size: 1389 86 1939
Fragments arranged by size:
1939 1389 86
PmlI CAC'GTG
Cuts at: 0 439 1908 3414
Size: 439 1469 1506
Fragments arranged by size:
1506 1469 439
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2905 2937 2959 2991 3414
Size: 2905 32 22 32 423
Fragments arranged by size:
2905 423 32 32 22
PpuMI rG'GwC_Cy
Cuts at: 0 2441 2526 2799 2934 3414
Size: 2441 85 273 135 480
Fragments arranged by size:
2441 480 273 135 85
PspOMI G'GGCC_C
Cuts at: 0 171 1884 2232 3414
Size: 171 1713 348 1182
Fragments arranged by size:
1713 1182 348 171
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2716 2748 3364 3396 3414
Size: 2716 32 616 32 18
Fragments arranged by size:
2716 616 32 32 18
PstI C_TGCA'G
Cuts at: 0 542 778 1125 2225 2429 2474 3414
Size: 542 236 347 1100 204 45 940
Fragments arranged by size:
1100 940 542 347 236 204 45
PvuII CAG'CTG
Cuts at: 0 1120 1225 1560 1851 1983 2257 2280 2865
Size: 1120 105 335 291 132 274 23 585
Cuts at: 2865 3403 3414
Size: 538 11
Fragments arranged by size:
1120 585 538 335 291 274 132 105
23 11
SacI G_AGCT'C
Cuts at: 0 3185 3414
Size: 3185 229
SalI G'TCGA_C
Cuts at: 0 1479 3414
Size: 1479 1935
SapI GCTCTTCn'nnn_
Cuts at: 0 469 3414
Size: 469 2945
SexAI A'CCwGG_T
Cuts at: 0 257 3414
Size: 257 3157
SfcI C'TryA_G
Cuts at: 0 538 576 774 1121 2221 2425 2470 3078
Size: 538 38 198 347 1100 204 45 608
Cuts at: 3078 3313 3414
Size: 235 101
Fragments arranged by size:
1100 608 538 347 235 204 198 101
45 38
SmaI CCC'GGG
Cuts at: 0 222 1716 3414
Size: 222 1494 1698
Fragments arranged by size:
1698 1494 222
SmlI C'TyrA_G
Cuts at: 0 2825 3414
Size: 2825 589
SphI G_CATG'C
Cuts at: 0 2154 2901 3330 3414
Size: 2154 747 429 84
Fragments arranged by size:
2154 747 429 84
SrfI GCCC'GGGC
Cuts at: 0 222 1716 3414
Size: 222 1494 1698
Fragments arranged by size:
1698 1494 222
StuI AGG'CCT
Cuts at: 0 772 2091 3346 3414
Size: 772 1319 1255 68
Fragments arranged by size:
1319 1255 772 68
StyI C'CwwG_G
Cuts at: 0 674 1193 1469 1706 3144 3414
Size: 674 519 276 237 1438 270
Fragments arranged by size:
1438 674 519 276 270 237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1001 1855 3414
Size: 1001 854 1559
Fragments arranged by size:
1559 1001 854
TatI w'GTAC_w
Cuts at: 0 426 3414
Size: 426 2988
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1098 1130 1346 1378 1608 1640 2656 2688
Size: 1098 32 216 32 230 32 1016 32
Cuts at: 2688 3414
Size: 726
Fragments arranged by size:
1098 1016 726 230 216 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 391 3414
Size: 391 3023
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 39 964 1200 2031 2501 3414
Size: 39 925 236 831 470 913
Fragments arranged by size:
925 913 831 470 236 39
XhoI C'TCGA_G
Cuts at: 0 2825 3414
Size: 2825 589
XmaI C'CCGG_G
Cuts at: 0 220 1714 3414
Size: 220 1494 1700
Fragments arranged by size:
1700 1494 220
XmnI GAAnn'nnTTC
Cuts at: 0 1132 2920 3414
Size: 1132 1788 494
Fragments arranged by size:
1788 1132 494
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BssHII BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I FspI FspAI
HaeII Hin4I HincII HindIII MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NspI PasI PciI PflMI PmlI
PpiI PpuMI PspOMI PsrI PstI PvuII SacI SalI
SapI SexAI SfcI SmaI SmlI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AhdI AleI ApoI AscI
AseI AsiSI AvrII BbeI BfrBI BglII BmtI BpuEI
BsaBI BsiWI BspEI BsrDI BsrGI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI EagI EcoNI EcoRI EcoRV
FalI FseI HpaI KasI KpnI MfeI MluI NarI
NdeI NheI NotI NruI NsiI PacI PfoI PmeI
PshAI PsiI PspXI PvuI RsrII SacII SanDI SbfI
ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SspI
SwaI XbaI ZraI