Restriction map for
pFN21AB9148
##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AlwI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspI AviII AvrII BamHI BanI
BbeI BcgI BclI BcnI BfrI
BglI BlnI BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI BsmFI BspDI
BspEI BsrBI BsrFI BssHII BssSI
BstBI BstEII BstPI BstXI Bsu36I
Cfr10I ClaI CpoI DraI DraIII
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoT14I EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MamI MluI MroI MslI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PflMI PinAI
PmeI PpuMI PshBI Psp1406I PvuI
RsrII SacI SacII SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StyI SwaI Tth111I
Van91I XbaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccII 1611 CGCG CG^CG
AcsI 1450 GAATTT [GA]^AATT[CT]
AflIII 580 ACATGT A^C[GA][CT]GT
AhdI 153 GACCACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 412 GTGCAC G^TGCAC
AlwNI 1070 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 412 GTGCAC G^TGCAC
ApoI 1450 GAATTT [GA]^AATT[CT]
Asp700I 805 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 153 GACCACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 125 CTCGAG C^[CT]CG[GA]G
BanII 1051 GAGCCC G[GA]GC[CT]^C
BbrPI 844 CACGTG CAC^GTG
BbsI 742 GAAGAC GAAGAC(2/6)
BbvCI 1120 CCTCAGC CCTCAGC(-5/-2)
BciVI 30 GGATAC GTATCC(6/5)
BfaI 1097 CTAG C^TAG
BpuAI 742 GAAGAC GAAGAC(2/6)
BsaAI 844 CACGTG [CT]AC^GT[GA]
BsaI 151 GAGACC GGTCTC(1/5)
BsaWI 1277 ACCGGA [AT]^CCGG[AT]
BsgI 841 CTGCAC GTGCAG(16/14)
BsmI 1184 GAATGC GAATGC(1/-1)
BsoBI 125 CTCGAG C^[CT]CG[GA]G
Bsp1407I 398 TGTACA T^GTACA
BspLU11I 580 ACATGT A^CATGT
BspMI 1627 ACCTGC ACCTGC(4/8)
BsrDI 555 CATTGC GCAATG(2/0)
BsrGI 398 TGTACA T^GTACA
Bst1107I 668 GTATAC GTA^TAC
BstAPI 611 GCAAGAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1611 CGCG CG^CG
BstZ17I 668 GTATAC GTA^TAC
DraII 787 AGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 745 GACAATCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 132 CCACGG C^C[GA][CT]GG
Eam1105I 153 GACCACCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 787 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRV 817 GATATC GAT^ATC
MaeI 1097 CTAG C^TAG
MaeIII 1003 GTCAC ^GT[ACGT]AC
MfeI 499 CAATTG C^AATTG
MunI 499 CAATTG C^AATTG
MvnI 1611 CGCG CG^CG
PaeR7I 125 CTCGAG C^TCGAG
PleI 128 GAGTC GAGTC(4/5)
PmaCI 844 CACGTG CAC^GTG
PmlI 844 CACGTG CAC^GTG
PshAI 577 GACACATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 601 CTGCAG CTGCA^G
SalI 754 GTCGAC G^TCGAC
SspBI 398 TGTACA T^GTACA
StuI 872 AGGCCT AGG^CCT
Tsp45I 1003 GTCAC ^GT[GC]AC
XhoI 125 CTCGAG C^TCGAG
XmnI 805 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 668 GTATAC GT^[AC][GT]AC
754 GTCGAC
AfaI 399 GTAC GT^AC
1551 GTAC
AvaII 942 GGACC G^G[AT]CC
1290 GGACC
BalI 718 TGGCCA TGG^CCA
771 TGGCCA
BglII 360 AGATCT A^GATCT
1256 AGATCT
BlpI 165 GCTGAGC GC^T[ACGT]AGC
170 GCTTAGC
BpmI 886 CTGGAG CTGGAG(16/14)
1330 CTGGAG
Bpu1102I 165 GCTGAGC GC^T[ACGT]AGC
170 GCTTAGC
BsaJI 132 CCACGG C^C[ACGT][ACGT]GG
945 CCGAGG
BspHI 585 TCATGA T^CATGA
678 TCATGA
BstYI 360 AGATCT [GA]^GATC[CT]
1256 AGATCT
CelII 165 GCTGAGC GC^T[ACGT]AGC
170 GCTTAGC
CfoI 852 GCGC GCG^C
1623 GCGC
DpnII 361 GATC ^GATC
1257 GATC
EaeI 718 TGGCCA [CT]^GGCC[GA]
771 TGGCCA
FauI 338 CCCGC CCCGC(4/6)
1299 GCGGG
HapII 1278 CCGG C^CGG
1635 CCGG
HhaI 852 GCGC GCG^C
1623 GCGC
HinP1I 852 GCGC G^CGC
1623 GCGC
HpaII 1278 CCGG C^CGG
1635 CCGG
MboI 361 GATC ^GATC
1257 GATC
MflI 360 AGATCT [GA]^GATC[CT]
1256 AGATCT
MluNI 718 TGGCCA TGG^CCA
771 TGGCCA
MscI 718 TGGCCA TGG^CCA
771 TGGCCA
MspA1I 163 CAGCTG C[AC]G^C[GT]G
419 CAGCTG
MspI 1278 CCGG C^CGG
1635 CCGG
NdeII 361 GATC ^GATC
1257 GATC
NlaIV 77 GGAACC GG[ACGT]^[ACGT]CC
1050 GGAGCC
PvuII 163 CAGCTG CAG^CTG
419 CAGCTG
RcaI 585 TCATGA T^CATGA
678 TCATGA
RsaI 399 GTAC GT^AC
1551 GTAC
Sau3AI 361 GATC ^GATC
1257 GATC
SmlI 125 CTCGAG C^T[CT][GA]AG
1007 CTCAAG
XhoII 360 AGATCT [GA]^GATC[CT]
1256 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AspHI BbvI BmyI
BseRI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI EarI EcoRII
Fnu4HI FokI HaeIII HincII HindII
HinfI HphI ItaI Ksp632I MaeII
MboII MnlI MseI MvaI MwoI
NlaIII NspI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I