(Circular) (Six-base) MAPSORT of: pFN21AB9148.seq Check: 5622 from: 1 to: 6349 pFN21AB9148 6349 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3641 3641 Size: 6349 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4159 278 Size: 53 83 186 936 2623 2468 Fragments arranged by size: 2623 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2674 2760 3692 1937 Size: 737 86 932 4594 Fragments arranged by size: 4594 932 737 86 Acc65I G'GTAC_C Cuts at: 3670 3670 Size: 6349 AclI AA'CG_TT Cuts at: 1563 4476 4849 1563 Size: 2913 373 3063 Fragments arranged by size: 3063 2913 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4412 5796 6064 1632 Size: 2780 1384 268 1917 Fragments arranged by size: 2780 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6152 AflIII A'CryG_T Cuts at: 1203 2585 5248 6225 1203 Size: 1382 2663 977 1327 Fragments arranged by size: 2663 1382 1327 977 AgeI A'CCGG_T Cuts at: 5180 5180 Size: 6349 AhdI GACnn_n'nnGTC Cuts at: 2163 5078 2163 Size: 2915 3434 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6349 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6349 AlwNI CAG_nnn'CTG Cuts at: 3080 4092 5664 3080 Size: 1012 1572 3765 Fragments arranged by size: 3765 1572 1012 ApaLI G'TGCA_C Cuts at: 2417 4406 5562 2417 Size: 1989 1156 3204 Fragments arranged by size: 3204 1989 1156 ApoI r'AATT_y Cuts at: 1493 3455 3658 3884 4036 6145 1493 Size: 1962 203 226 152 2109 1697 Fragments arranged by size: 2109 1962 1697 226 203 152 AseI AT'TA_AT Cuts at: 160 1032 4903 160 Size: 872 3871 1606 Fragments arranged by size: 3871 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6349 AvaI C'yCGr_G Cuts at: 1870 1943 2130 3674 1870 Size: 73 187 1544 4545 Fragments arranged by size: 4545 1544 187 73 BamHI G'GATC_C Cuts at: 3679 4046 3679 Size: 367 5982 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3670 5125 618 Size: 325 217 627 1883 1455 1842 Fragments arranged by size: 1883 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3060 3668 1391 Size: 1669 608 4072 Fragments arranged by size: 4072 1669 608 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2754 928 Size: 971 855 4523 Fragments arranged by size: 4523 971 855 BbvCI CC'TCA_GC Cuts at: 3126 3126 Size: 6349 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4540 4574 1903 Size: 34 2603 34 3678 Fragments arranged by size: 3678 2603 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2029 4241 5451 2029 Size: 2212 1210 2927 Fragments arranged by size: 2927 2212 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6349 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4960 136 Size: 107 122 71 631 605 3288 1525 Fragments arranged by size: 3288 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2365 3261 6344 2365 Size: 896 3083 2370 Fragments arranged by size: 3083 2370 896 BlpI GC'TnA_GC Cuts at: 2171 2176 3766 2171 Size: 5 1590 4754 Fragments arranged by size: 4754 1590 5 Bme1580I G_kGCm'C Cuts at: 1790 2321 2421 4410 5566 1790 Size: 531 100 1989 1156 2573 Fragments arranged by size: 2573 1989 1156 531 100 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6349 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4080 5038 442 Size: 416 53 83 399 24 2663 958 1753 Fragments arranged by size: 2663 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6349 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2912 3356 5009 1239 Size: 1673 444 1653 2579 Fragments arranged by size: 2579 1673 1653 444 Bpu10I CC'TnA_GC Cuts at: 3126 3126 Size: 6349 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2997 4474 5339 5637 2997 Size: 1477 865 298 3709 Fragments arranged by size: 3709 1477 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2150 5012 882 Size: 617 651 2862 2219 Fragments arranged by size: 2862 2219 651 617 BsaAI yAC'GTr Cuts at: 493 1204 2851 4140 493 Size: 711 1647 1289 2702 Fragments arranged by size: 2702 1647 1289 711 BsaBI GATnn'nnATC Cuts at: 6343 6343 Size: 6349 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4156 4538 5173 275 Size: 53 83 186 936 2623 382 635 1451 Fragments arranged by size: 2623 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3282 4782 5180 5454 5601 6012 3282 Size: 1500 398 274 147 411 3619 Fragments arranged by size: 3619 1500 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3539 3569 1178 Size: 30 165 30 2136 30 3958 Fragments arranged by size: 3958 2136 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2971 3557 3560 1186 Size: 1785 586 3 3975 Fragments arranged by size: 3975 1785 586 3 BseYI C'CCAG_C Cuts at: 5552 6048 5552 Size: 496 5853 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2831 1163 Size: 573 1095 4681 Fragments arranged by size: 4681 1095 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3799 4560 5588 1540 Size: 275 186 1798 761 1028 2301 Fragments arranged by size: 2301 1798 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2421 3206 3609 3624 4410 4495 5566 2421 Size: 785 403 15 786 85 1071 3204 Fragments arranged by size: 3204 1071 786 785 403 85 15 BsmI GAATG_Cn' Cuts at: 3195 3860 3953 3195 Size: 665 93 5591 Fragments arranged by size: 5591 665 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2321 2421 3060 3206 3609 3624 3668 Size: 399 531 100 639 146 403 15 44 Cuts at: 3668 4410 4495 5566 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 639 531 403 399 146 100 85 44 15 BspEI T'CCGG_A Cuts at: 6012 6012 Size: 6349 BspHI T'CATG_A Cuts at: 2590 2683 4238 5968 2590 Size: 93 1555 1730 2971 Fragments arranged by size: 2971 1730 1555 93 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3641 3704 844 Size: 2797 63 3489 Fragments arranged by size: 3489 2797 63 BsrBI CCG'CTC Cuts at: 1394 1823 4236 1394 Size: 429 2413 3507 Fragments arranged by size: 3507 2413 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2559 4844 5018 65 Size: 1183 1311 2285 174 1396 Fragments arranged by size: 2285 1396 1311 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4993 5180 1700 Size: 110 3183 187 2869 Fragments arranged by size: 3183 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2403 96 Size: 2307 4042 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6349 BssSI C'ACGA_G Cuts at: 4409 5421 4409 Size: 1012 5337 BstAPI GCAn_nnn'nTGC Cuts at: 794 2622 794 Size: 1828 4521 BstBI TT'CG_AA Cuts at: 5153 5153 Size: 6349 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6349 BstYI r'GATC_y Cuts at: 1972 2365 3261 3679 4046 4431 4448 5900 5986 Size: 393 896 418 367 385 17 1452 86 Cuts at: 5986 5998 6344 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 896 418 393 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2675 2675 Size: 6349 BtgI C'CryG_G Cuts at: 513 1065 2004 2137 513 Size: 552 939 133 4725 Fragments arranged by size: 4725 939 552 133 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5610 Fragments arranged by size: 5610 683 56 BtsI GCAGTG_nn' Cuts at: 3530 3559 3865 4659 4679 3530 Size: 29 306 794 20 5200 Fragments arranged by size: 5200 794 306 29 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6349 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6314 DraI TTT'AAA Cuts at: 3653 3653 Size: 6349 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2756 5356 809 Size: 507 1440 2600 1802 Fragments arranged by size: 2600 1802 1440 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2723 2776 3796 Size: 54 1425 50 275 18 893 53 1020 Cuts at: 3796 4685 6314 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1020 893 889 275 54 53 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3796 1537 Size: 275 1984 4090 Fragments arranged by size: 4090 1984 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2478 2955 3184 4279 1353 Size: 1125 477 229 1095 3423 Fragments arranged by size: 3423 1125 1095 477 229 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2048 4936 5310 5456 387 Size: 1064 138 459 2888 374 146 1280 Fragments arranged by size: 2888 1280 1064 459 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2912 3356 4412 5009 5796 6064 1239 Size: 393 1280 444 1056 597 787 268 1524 Fragments arranged by size: 1524 1280 1056 787 597 444 393 268 EcoO109I rG'GnC_Cy Cuts at: 2793 2793 Size: 6349 EcoRI G'AATT_C Cuts at: 3658 4036 3658 Size: 378 5971 EcoRV GAT'ATC Cuts at: 2824 2824 Size: 6349 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2210 2641 3195 2210 Size: 431 554 5364 Fragments arranged by size: 5364 554 431 FspI TGC'GCA Cuts at: 4855 4855 Size: 6349 HaeII r_GCGC'y Cuts at: 1678 5496 1678 Size: 3818 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2149 2181 2322 2354 2954 Size: 32 135 32 577 32 141 32 600 Cuts at: 2954 2986 4990 5022 5064 5096 1373 Size: 32 2004 32 42 32 2626 Fragments arranged by size: 2626 2004 600 577 141 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2761 2833 3153 3693 3939 669 Size: 988 281 823 72 320 540 246 3079 Fragments arranged by size: 3079 988 823 540 320 281 246 72 HindIII A'AGCT_T Cuts at: 748 748 Size: 6349 HpaI GTT'AAC Cuts at: 3939 3939 Size: 6349 KpnI G_GTAC'C Cuts at: 3674 3674 Size: 6349 MfeI C'AATT_G Cuts at: 2504 3948 2504 Size: 1444 4905 MluI A'CGCG_T Cuts at: 6225 6225 Size: 6349 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2849 3273 5463 5647 1305 Size: 1544 424 2190 184 2007 Fragments arranged by size: 2190 2007 1544 424 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2725 2778 10 Size: 54 1425 343 893 53 3581 Fragments arranged by size: 3581 1425 893 343 54 53 MslI CAynn'nnrTG Cuts at: 518 1166 4307 4666 4825 518 Size: 648 3141 359 159 2042 Fragments arranged by size: 3141 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2170 2426 3765 4442 5590 5835 1606 Size: 564 256 1339 677 1148 245 2120 Fragments arranged by size: 2120 1339 1148 677 564 256 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4858 Fragments arranged by size: 4858 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6349 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6349 NotI GC'GGCC_GC Cuts at: 3796 3796 Size: 6349 NspI r_CATG'y Cuts at: 1132 1229 2589 3241 3403 3707 5252 1132 Size: 97 1360 652 162 304 1545 2229 Fragments arranged by size: 2229 1545 1360 652 304 162 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6349 PciI A'CATG_T Cuts at: 2585 5248 2585 Size: 2663 3686 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6349 PmeI GTTT'AAAC Cuts at: 3653 3653 Size: 6349 PmlI CAC'GTG Cuts at: 2851 2851 Size: 6349 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4418 4450 5931 5963 4418 Size: 32 1481 32 4804 Fragments arranged by size: 4804 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2586 1613 Size: 973 5376 PsiI TTA'TAA Cuts at: 3919 3919 Size: 6349 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3563 3595 3563 Size: 32 6317 PstI C_TGCA'G Cuts at: 830 2610 3701 830 Size: 1780 1091 3478 Fragments arranged by size: 3478 1780 1091 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6349 PvuII CAG'CTG Cuts at: 2170 2426 2170 Size: 256 6093 SalI G'TCGA_C Cuts at: 1936 2759 3691 1936 Size: 823 932 4594 Fragments arranged by size: 4594 932 823 SbfI CC_TGCA'GG Cuts at: 3701 3701 Size: 6349 ScaI AGT'ACT Cuts at: 4597 6309 4597 Size: 1712 4637 SfcI C'TryA_G Cuts at: 826 1045 2361 2606 3390 3697 4832 5513 5704 Size: 219 1316 245 784 307 1135 681 191 Cuts at: 5704 826 Size: 1471 Fragments arranged by size: 1471 1316 1135 784 681 307 245 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6349 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6349 SmaI CCC'GGG Cuts at: 1872 3676 1872 Size: 1804 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2130 3012 4453 5354 5616 820 Size: 197 926 187 882 1441 901 262 1553 Fragments arranged by size: 1553 1441 926 901 882 262 197 187 SnaBI TAC'GTA Cuts at: 493 4140 493 Size: 3647 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6349 SphI G_CATG'C Cuts at: 1132 3707 1132 Size: 2575 3774 SspI AAT'ATT Cuts at: 5 52 4273 5 Size: 47 4221 2081 Fragments arranged by size: 4221 2081 47 StuI AGG'CCT Cuts at: 1517 2879 1517 Size: 1362 4987 StyI C'CwwG_G Cuts at: 513 1065 2004 3788 513 Size: 552 939 1784 3074 Fragments arranged by size: 3074 1784 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2964 3221 4376 4393 4546 4731 5176 Size: 1255 1384 257 1155 17 153 185 445 Cuts at: 5176 325 Size: 1498 Fragments arranged by size: 1498 1384 1255 1155 445 257 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2403 4595 6307 Size: 274 80 33 51 1443 426 2192 1712 Cuts at: 6307 96 Size: 138 Fragments arranged by size: 2192 1712 1443 426 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6309 6341 6309 Size: 32 6317 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6349 XbaI T'CTAG_A Cuts at: 3685 3685 Size: 6349 XhoI C'TCGA_G Cuts at: 1943 2130 1943 Size: 187 6162 XmaI C'CCGG_G Cuts at: 1870 3674 1870 Size: 1804 4545 XmnI GAAnn'nnTTC Cuts at: 2814 4478 2814 Size: 1664 4685 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4157 276 Size: 53 83 186 936 2623 2468 Fragments arranged by size: 2623 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI