Restriction map for
pFN21AB9148
(Linear) (Six-base) MAPSORT of: KIBB9148.seq Check: 3060 from: 1 to: 1644
KIBB9148.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1636 1644
Size: 1636 8
AccI GT'mk_AC
Cuts at: 0 669 755 1644
Size: 669 86 889
Fragments arranged by size:
889 669 86
AflIII A'CryG_T
Cuts at: 0 580 1644
Size: 580 1064
AhdI GACnn_n'nnGTC
Cuts at: 0 158 1644
Size: 158 1486
AlwNI CAG_nnn'CTG
Cuts at: 0 1075 1644
Size: 1075 569
ApaLI G'TGCA_C
Cuts at: 0 412 1644
Size: 412 1232
ApoI r'AATT_y
Cuts at: 0 1450 1644
Size: 1450 194
AvaI C'yCGr_G
Cuts at: 0 125 1644
Size: 125 1519
BanII G_rGCy'C
Cuts at: 0 1055 1644
Size: 1055 589
BbsI GAAGACnn'nnnn_
Cuts at: 0 749 1644
Size: 749 895
BbvCI CC'TCA_GC
Cuts at: 0 1121 1644
Size: 1121 523
BciVI GTATCCnnnnn_n'
Cuts at: 0 24 1644
Size: 24 1620
BglII A'GATC_T
Cuts at: 0 360 1256 1644
Size: 360 896 388
Fragments arranged by size:
896 388 360
BlpI GC'TnA_GC
Cuts at: 0 166 171 1644
Size: 166 5 1473
Fragments arranged by size:
1473 166 5
Bme1580I G_kGCm'C
Cuts at: 0 316 416 1644
Size: 316 100 1228
Fragments arranged by size:
1228 316 100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 907 1351 1644
Size: 907 444 293
Fragments arranged by size:
907 444 293
Bpu10I CC'TnA_GC
Cuts at: 0 1121 1644
Size: 1121 523
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 992 1644
Size: 992 652
BsaI GGTCTCn'nnnn_
Cuts at: 0 145 1644
Size: 145 1499
BsaAI yAC'GTr
Cuts at: 0 846 1644
Size: 846 798
BsaWI w'CCGG_w
Cuts at: 0 1277 1644
Size: 1277 367
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1534 1564 1644
Size: 1534 30 80
Fragments arranged by size:
1534 80 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 966 1552 1555 1644
Size: 966 586 3 89
Fragments arranged by size:
966 586 89 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 826 1644
Size: 826 818
BsiHKAI G_wGCw'C
Cuts at: 0 416 1201 1604 1619 1644
Size: 416 785 403 15 25
Fragments arranged by size:
785 416 403 25 15
BsmI GAATG_Cn'
Cuts at: 0 1190 1644
Size: 1190 454
Bsp1286I G_dGCh'C
Cuts at: 0 316 416 1055 1201 1604 1619 1644
Size: 316 100 639 146 403 15 25
Fragments arranged by size:
639 403 316 146 100 25 15
BspHI T'CATG_A
Cuts at: 0 585 678 1644
Size: 585 93 966
Fragments arranged by size:
966 585 93
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1636 1644
Size: 1636 8
BsrDI GCAATG_nn'
Cuts at: 0 554 1644
Size: 554 1090
BsrGI T'GTAC_A
Cuts at: 0 398 1644
Size: 398 1246
BstAPI GCAn_nnn'nTGC
Cuts at: 0 617 1644
Size: 617 1027
BstYI r'GATC_y
Cuts at: 0 360 1256 1644
Size: 360 896 388
Fragments arranged by size:
896 388 360
BstZ17I GTA'TAC
Cuts at: 0 670 1644
Size: 670 974
BtgI C'CryG_G
Cuts at: 0 132 1644
Size: 132 1512
BtsI GCAGTG_nn'
Cuts at: 0 1525 1554 1644
Size: 1525 29 90
Fragments arranged by size:
1525 90 29
DrdI GACnn_nn'nnGTC
Cuts at: 0 751 1644
Size: 751 893
EaeI y'GGCC_r
Cuts at: 0 718 771 1644
Size: 718 53 873
Fragments arranged by size:
873 718 53
EarI CTCTTCn'nnn_
Cuts at: 0 473 950 1179 1644
Size: 473 477 229 465
Fragments arranged by size:
477 473 465 229
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 43 1644
Size: 43 1601
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 907 1351 1644
Size: 907 444 293
Fragments arranged by size:
907 444 293
EcoO109I rG'GnC_Cy
Cuts at: 0 788 1644
Size: 788 856
EcoRV GAT'ATC
Cuts at: 0 819 1644
Size: 819 825
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 205 636 1190 1644
Size: 205 431 554 454
Fragments arranged by size:
554 454 431 205
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 144 176 317 349 949 981 1644
Size: 144 32 141 32 600 32 663
Fragments arranged by size:
663 600 144 141 32 32 32
HincII GTy'rAC
Cuts at: 0 756 828 1148 1644
Size: 756 72 320 496
Fragments arranged by size:
756 496 320 72
MfeI C'AATT_G
Cuts at: 0 499 1644
Size: 499 1145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 844 1268 1644
Size: 844 424 376
Fragments arranged by size:
844 424 376
MscI TGG'CCA
Cuts at: 0 720 773 1644
Size: 720 53 871
Fragments arranged by size:
871 720 53
MspA1I CmG'CkG
Cuts at: 0 165 421 1644
Size: 165 256 1223
Fragments arranged by size:
1223 256 165
NspI r_CATG'y
Cuts at: 0 584 1236 1398 1644
Size: 584 652 162 246
Fragments arranged by size:
652 584 246 162
PciI A'CATG_T
Cuts at: 0 580 1644
Size: 580 1064
PmlI CAC'GTG
Cuts at: 0 846 1644
Size: 846 798
PshAI GACnn'nnGTC
Cuts at: 0 581 1644
Size: 581 1063
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1558 1590 1644
Size: 1558 32 54
Fragments arranged by size:
1558 54 32
PstI C_TGCA'G
Cuts at: 0 605 1644
Size: 605 1039
PvuII CAG'CTG
Cuts at: 0 165 421 1644
Size: 165 256 1223
Fragments arranged by size:
1223 256 165
SalI G'TCGA_C
Cuts at: 0 754 1644
Size: 754 890
SfcI C'TryA_G
Cuts at: 0 356 601 1385 1644
Size: 356 245 784 259
Fragments arranged by size:
784 356 259 245
SmlI C'TyrA_G
Cuts at: 0 125 1007 1644
Size: 125 882 637
Fragments arranged by size:
882 637 125
StuI AGG'CCT
Cuts at: 0 874 1644
Size: 874 770
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 959 1216 1644
Size: 959 257 428
Fragments arranged by size:
959 428 257
TatI w'GTAC_w
Cuts at: 0 398 1644
Size: 398 1246
XhoI C'TCGA_G
Cuts at: 0 125 1644
Size: 125 1519
XmnI GAAnn'nnTTC
Cuts at: 0 809 1644
Size: 809 835
Enzymes that do cut:
AarI AccI AflIII AhdI AlwNI ApaLI ApoI AvaI
BanII BbsI BbvCI BciVI BglII BlpI Bme1580I BpmI
Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI BstAPI
BstYI BstZ17I BtgI BtsI DrdI EaeI EarI EciI
Eco57MI EcoO109I EcoRV FalI Hin4I HincII MfeI MmeI
MscI MspA1I NspI PciI PmlI PshAI PsrI PstI
PvuII SalI SfcI SmlI StuI TaqII TatI XhoI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AleI
AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BcgI BclI BfrBI BglI BmgBI
BmrI BmtI BplI BsaBI BsaHI BseYI BsiEI BsiWI
BsmBI BspEI BsrBI BsrFI BssHII BssSI BstBI BstEII
BstXI Bsu36I BtgZI ClaI CspCI DraI DraIII EagI
EcoICRI EcoNI EcoRI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MluI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PpiI PpuMI PsiI PspOMI PspXI
PvuI RsrII SacI SacII SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StyI SwaI TstI Tth111I XbaI
XcmI XmaI ZraI