Restriction map for
pFN21AB9131
##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AvaI AviII AvrII
BalI BanI BbeI BbrPI BbvCI
BcgI BcnI BfaI BfrI BglI
BlnI BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstZ17I
Bsu36I CfoI Cfr10I CpoI DraIII
DrdI DsaI EaeI EagI EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
EcoT14I FauI FseI FspI HaeII
HapII HhaI HinP1I HindIII HpaI
HpaII KasI KpnI KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI StyI
Van91I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AciI 902 GCGG CCGC(-3/-1)
AcyI 327 GGCGTC G[GA]^CG[CT]C
AhdI 258 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 258 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1297 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1610 GGACC G^G[AT]CC
BamHI 1176 GGATCC G^GATCC
BbsI 585 GAAGAC GAAGAC(2/6)
BciVI 786 GGATAC GTATCC(6/5)
BclI 894 TGATCA T^GATCA
BpuAI 585 GAAGAC GAAGAC(2/6)
BsaHI 327 GGCGTC G[GA]^CG[CT]C
BsmAI 1164 GTCTC GTCTC(1/5)
BsmFI 1321 GGGAC GGGAC(10/14)
BspDI 1544 ATCGAT AT^CGAT
BstEII 768 GGTGACC G^GT[ACGT]ACC
BstPI 768 GGTGACC G^GT[ACGT]ACC
Cfr13I 1610 GGACC G^G[ACGT]CC
ClaI 1544 ATCGAT AT^CGAT
DraII 1609 AGGACCT [GA]G^G[ACGT]CC[CT]
Eam1105I 258 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1308 CCTTTTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1609 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 768 GGTGACC G^GT[ACGT]ACC
FbaI 894 TGATCA T^GATCA
HgaI 328 GCGTC GACGC(5/10)
Hin1I 327 GGCGTC G[GA]^CG[CT]C
NdeI 1169 CATATG CA^TATG
NlaIV 1176 GGATCC GG[ACGT]^[ACGT]CC
PflFI 1297 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1609 AGGACCT [GA]G^G[AT]CC[CT]
PstI 1538 CTGCAG CTGCA^G
SapI 719 GCTCTTC GCTCTTC(1/4)
Sau96I 1610 GGACC G^G[ACGT]CC
SexAI 1298 ACCAGGT A^CC[AT]GGT
SmlI 54 CTCAAG C^T[CT][GA]AG
SphI 833 GCATGC GCATG^C
SspI 184 AATATT AAT^ATT
StuI 1640 AGGCCT AGG^CCT
SwaI 884 ATTTAAAT ATTT^AAAT
Tth111I 1297 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 686 CCAAGCCCCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 245 GTGCAC G^TGCAC
1601 GTGCAC
ApaLI 245 GTGCAC G^TGCAC
1601 GTGCAC
Asp700I 90 GAATAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
853 GAACGGATTC
BanII 776 GAGCTC G[GA]GC[CT]^C
1711 GAGCTC
BglII 1281 AGATCT A^GATCT
1677 AGATCT
BlpI 47 GCTCAGC GC^T[ACGT]AGC
912 GCTTAGC
Bpu1102I 47 GCTCAGC GC^T[ACGT]AGC
912 GCTTAGC
BsmI 9 GAATGC GAATGC(1/-1)
1136 GAATGC
BspHI 645 TCATGA T^CATGA
891 TCATGA
BstXI 603 CCAGTTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
863 CCAAGAACATGG
CelII 47 GCTCAGC GC^T[ACGT]AGC
912 GCTTAGC
EcoT22I 982 ATGCAT ATGCA^T
1138 ATGCAT
HaeIII 1641 GGCC GG^CC
1719 GGCC
HincII 1303 GTCAAC GT[CT]^[GA]AC
1564 GTCAAC
HindII 1303 GTCAAC GT[CT]^[GA]AC
1564 GTCAAC
HinfI 335 GATTC G^A[ACGT]TC
858 GATTC
MaeII 1374 ACGT A^CGT
1562 ACGT
MwoI 658 GCCTTGCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
681 GCGACCCAAGC
NsiI 982 ATGCAT ATGCA^T
1138 ATGCAT
NspI 833 GCATGC [GA]CATG^[CT]
1697 ACATGC
RcaI 645 TCATGA T^CATGA
891 TCATGA
SacI 776 GAGCTC GAGCT^C
1711 GAGCTC
SfcI 930 CTGTAG C^T[GA][CT]AG
1538 CTGCAG
TfiI 335 GATTC G^A[AT]TC
858 GATTC
XmnI 90 GAATAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
853 GAACGGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AspHI BbvI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I DdeI DpnII DraI
EarI EcoRII Fnu4HI FokI HphI
ItaI Ksp632I MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI NdeII NlaIII PvuII RsaI
Sau3AI ScrFI SfaNI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII