(Circular) (Six-base) MAPSORT of: pFN21AB9131.seq Check: 54 from: 1 to: 6442 pFN21AB9131 6442 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4252 278 Size: 53 83 186 936 2716 2468 Fragments arranged by size: 2716 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3785 1937 Size: 1848 4594 Acc65I G'GTAC_C Cuts at: 3763 3763 Size: 6442 AclI AA'CG_TT Cuts at: 1563 4569 4942 1563 Size: 3006 373 3063 Fragments arranged by size: 3063 3006 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2447 4505 5889 6157 1632 Size: 815 2058 1384 268 1917 Fragments arranged by size: 2058 1917 1384 815 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6245 AflIII A'CryG_T Cuts at: 1203 5341 6318 1203 Size: 4138 977 1327 Fragments arranged by size: 4138 1327 977 AgeI A'CCGG_T Cuts at: 5273 5273 Size: 6442 AhdI GACnn_n'nnGTC Cuts at: 2268 5171 2268 Size: 2903 3539 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6442 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6442 AlwNI CAG_nnn'CTG Cuts at: 4185 5757 4185 Size: 1572 4870 ApaLI G'TGCA_C Cuts at: 2250 3606 4499 5655 2250 Size: 1356 893 1156 3037 Fragments arranged by size: 3037 1356 1156 893 ApoI r'AATT_y Cuts at: 1493 2288 3215 3257 3467 3751 3977 4129 6238 Size: 795 927 42 210 284 226 152 2109 Cuts at: 6238 1493 Size: 1697 Fragments arranged by size: 2109 1697 927 795 284 226 210 152 42 AseI AT'TA_AT Cuts at: 160 1032 4996 160 Size: 872 3964 1606 Fragments arranged by size: 3964 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6442 AvaI C'yCGr_G Cuts at: 1870 1943 3767 1870 Size: 73 1824 4545 Fragments arranged by size: 4545 1824 73 BamHI G'GATC_C Cuts at: 3181 3772 4139 3181 Size: 591 367 5484 Fragments arranged by size: 5484 591 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3763 5218 618 Size: 325 217 627 1976 1455 1842 Fragments arranged by size: 1976 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2785 3720 3761 1391 Size: 1394 935 41 4072 Fragments arranged by size: 4072 1394 935 41 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2597 928 Size: 971 698 4773 Fragments arranged by size: 4773 971 698 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4633 4667 1903 Size: 34 2696 34 3678 Fragments arranged by size: 3678 2696 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2785 4334 5544 2785 Size: 1549 1210 3683 Fragments arranged by size: 3683 1549 1210 BclI T'GATC_A Cuts at: 1548 2899 1548 Size: 1351 5091 BfrBI ATG'CAT Cuts at: 2989 3145 2989 Size: 156 6286 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5053 136 Size: 107 122 71 631 605 3381 1525 Fragments arranged by size: 3381 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3286 3682 6437 3286 Size: 396 2755 3291 Fragments arranged by size: 3291 2755 396 BlpI GC'TnA_GC Cuts at: 2053 2918 3859 2053 Size: 865 941 4636 Fragments arranged by size: 4636 941 865 Bme1580I G_kGCm'C Cuts at: 1790 2254 3610 4503 5659 1790 Size: 464 1356 893 1156 2573 Fragments arranged by size: 2573 1356 1156 893 464 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6442 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4173 5131 442 Size: 416 53 83 399 24 2756 958 1753 Fragments arranged by size: 2756 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6442 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3186 3249 3186 Size: 63 6379 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2723 3153 3219 5102 1239 Size: 1484 430 66 1883 2579 Fragments arranged by size: 2579 1883 1484 430 66 Bpu10I CC'TnA_GC Cuts at: 2544 2544 Size: 6442 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2044 4567 5432 5730 2044 Size: 2523 865 298 2756 Fragments arranged by size: 2756 2523 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5105 882 Size: 617 3606 2219 Fragments arranged by size: 3606 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4233 493 Size: 711 3029 2702 Fragments arranged by size: 3029 2702 711 BsaBI GATnn'nnATC Cuts at: 6436 6436 Size: 6442 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2333 4249 4631 5266 Size: 53 83 186 936 800 1916 382 635 Cuts at: 5266 275 Size: 1451 Fragments arranged by size: 1916 1451 936 800 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4875 5273 5547 5694 6105 4875 Size: 398 274 147 411 5212 Fragments arranged by size: 5212 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6217 Fragments arranged by size: 6217 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2101 2104 2721 2868 3728 1186 Size: 915 3 617 147 860 3900 Fragments arranged by size: 3900 915 860 617 147 3 BseYI C'CCAG_C Cuts at: 3323 5645 6141 3323 Size: 2322 496 3624 Fragments arranged by size: 3624 2322 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5869 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3892 4653 5681 1540 Size: 275 186 1891 761 1028 2301 Fragments arranged by size: 2301 1891 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2254 2785 3610 3720 4503 4588 5659 2254 Size: 531 825 110 783 85 1071 3037 Fragments arranged by size: 3037 1071 825 783 531 110 85 BsmI GAATG_Cn' Cuts at: 2020 3147 3953 4046 2020 Size: 1127 806 93 4416 Fragments arranged by size: 4416 1127 806 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2254 2785 3610 3720 3761 4503 4588 Size: 399 464 531 825 110 41 742 85 Cuts at: 4588 5659 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 825 742 531 464 399 110 85 41 BspEI T'CCGG_A Cuts at: 6105 6105 Size: 6442 BspHI T'CATG_A Cuts at: 2650 2896 4331 6061 2650 Size: 246 1435 1730 3031 Fragments arranged by size: 3031 1730 1435 246 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3797 844 Size: 2953 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4329 1394 Size: 429 2506 3507 Fragments arranged by size: 3507 2506 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2483 2592 2902 2917 4937 5111 65 Size: 1183 1235 109 310 15 2020 174 1396 Fragments arranged by size: 2020 1396 1235 1183 310 174 109 15 BsrFI r'CCGG_y Cuts at: 1700 1810 5086 5273 1700 Size: 110 3276 187 2869 Fragments arranged by size: 3276 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6442 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6442 BssSI C'ACGA_G Cuts at: 4502 5514 4502 Size: 1012 5430 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6442 BstBI TT'CG_AA Cuts at: 5246 5246 Size: 6442 BstEII G'GTnAC_C Cuts at: 1184 2773 1184 Size: 1589 4853 BstXI CCAn_nnnn'nTGG Cuts at: 2615 2875 2615 Size: 260 6182 BstYI r'GATC_y Cuts at: 1972 3181 3286 3445 3682 3772 4139 4524 4541 Size: 1209 105 159 237 90 367 385 17 Cuts at: 4541 5993 6079 6091 6437 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1209 385 367 346 237 159 105 90 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4951 Fragments arranged by size: 4951 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5703 Fragments arranged by size: 5703 683 56 BtsI GCAGTG_nn' Cuts at: 3246 3958 4752 4772 3246 Size: 712 794 20 4916 Fragments arranged by size: 4916 794 712 20 ClaI AT'CG_AT Cuts at: 1554 3550 1554 Size: 1996 4446 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6407 DraI TTT'AAA Cuts at: 2892 3318 3696 3730 3746 2892 Size: 426 378 34 16 5588 Fragments arranged by size: 5588 426 378 34 16 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5449 809 Size: 507 4133 1802 Fragments arranged by size: 4133 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3889 4778 6407 Size: 54 1425 50 275 18 2059 889 1629 Cuts at: 6407 8 Size: 43 Fragments arranged by size: 2059 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3889 1537 Size: 275 2077 4090 Fragments arranged by size: 4090 2077 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2122 2731 3501 4372 1353 Size: 769 609 770 871 3423 Fragments arranged by size: 3423 871 770 769 609 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5029 5403 5549 387 Size: 1064 138 3440 374 146 1280 Fragments arranged by size: 3440 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2783 3718 2783 Size: 935 5507 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2447 2723 3153 3219 4505 5102 5889 Size: 393 815 276 430 66 1286 597 787 Cuts at: 5889 6157 1239 Size: 268 1524 Fragments arranged by size: 1524 1286 815 787 597 430 393 276 268 66 EcoNI CCTnn'n_nnAGG Cuts at: 3317 3317 Size: 6442 EcoO109I rG'GnC_Cy Cuts at: 3615 3615 Size: 6442 EcoRI G'AATT_C Cuts at: 3751 4129 3751 Size: 378 6064 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2398 2812 2398 Size: 414 6028 FspI TGC'GCA Cuts at: 4948 4948 Size: 6442 HaeII r_GCGC'y Cuts at: 1678 5589 1678 Size: 3911 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2254 2286 2318 2350 2767 Size: 32 135 32 682 32 32 32 417 Cuts at: 2767 2799 3189 3221 3413 3445 3674 3706 5083 Size: 32 390 32 192 32 229 32 1377 Cuts at: 5083 5115 5157 5189 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1377 682 417 390 229 192 135 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3310 3571 3786 4032 669 Size: 988 281 1372 261 215 246 3079 Fragments arranged by size: 3079 1372 988 281 261 246 215 HindIII A'AGCT_T Cuts at: 748 748 Size: 6442 HpaI GTT'AAC Cuts at: 4032 4032 Size: 6442 KpnI G_GTAC'C Cuts at: 3767 3767 Size: 6442 MfeI C'AATT_G Cuts at: 4041 4041 Size: 6442 MluI A'CGCG_T Cuts at: 6318 6318 Size: 6442 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2485 3517 5556 5740 1305 Size: 1180 1032 2039 184 2007 Fragments arranged by size: 2039 2007 1180 1032 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4620 Fragments arranged by size: 4620 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2277 2958 3639 4400 4759 4918 Size: 648 843 268 681 681 761 359 159 Cuts at: 4918 518 Size: 2042 Fragments arranged by size: 2042 843 761 681 681 648 359 268 159 MspA1I CmG'CkG Cuts at: 1606 2683 2831 3527 3858 4535 5683 5928 1606 Size: 1077 148 696 331 677 1148 245 2120 Fragments arranged by size: 2120 1148 1077 696 677 331 245 148 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4951 Fragments arranged by size: 4951 939 552 NdeI CA'TA_TG Cuts at: 387 3175 387 Size: 2788 3654 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6442 NotI GC'GGCC_GC Cuts at: 3889 3889 Size: 6442 NsiI A_TGCA'T Cuts at: 2991 3147 2991 Size: 156 6286 NspI r_CATG'y Cuts at: 1132 1229 2842 3706 3800 5345 1132 Size: 97 1613 864 94 1545 2229 Fragments arranged by size: 2229 1613 1545 864 97 94 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6442 PciI A'CATG_T Cuts at: 5341 5341 Size: 6442 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6442 PmeI GTTT'AAAC Cuts at: 3746 3746 Size: 6442 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2508 2540 4511 4543 6024 6056 2508 Size: 32 1971 32 1481 32 2894 Fragments arranged by size: 2894 1971 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3615 3615 Size: 6442 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6442 PsiI TTA'TAA Cuts at: 4012 4012 Size: 6442 PstI C_TGCA'G Cuts at: 830 3547 3794 830 Size: 2717 247 3478 Fragments arranged by size: 3478 2717 247 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6442 PvuII CAG'CTG Cuts at: 2683 2831 3527 2683 Size: 148 696 5598 Fragments arranged by size: 5598 696 148 SacI G_AGCT'C Cuts at: 2785 3720 2785 Size: 935 5507 SalI G'TCGA_C Cuts at: 1936 3784 1936 Size: 1848 4594 SapI GCTCTTCn'nnn_ Cuts at: 2731 2731 Size: 6442 SbfI CC_TGCA'GG Cuts at: 3794 3794 Size: 6442 ScaI AGT'ACT Cuts at: 4690 6402 4690 Size: 1712 4730 SexAI A'CCwGG_T Cuts at: 3303 3303 Size: 6442 SfcI C'TryA_G Cuts at: 826 1045 2935 3543 3790 4925 5606 5797 826 Size: 219 1890 608 247 1135 681 191 1471 Fragments arranged by size: 1890 1471 1135 681 608 247 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6442 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6442 SmaI CCC'GGG Cuts at: 1872 3769 1872 Size: 1897 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2059 4546 5447 5709 820 Size: 197 926 116 2487 901 262 1553 Fragments arranged by size: 2487 1553 926 901 262 197 116 SnaBI TAC'GTA Cuts at: 493 4233 493 Size: 3740 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6442 SphI G_CATG'C Cuts at: 1132 2842 3800 1132 Size: 1710 958 3774 Fragments arranged by size: 3774 1710 958 SspI AAT'ATT Cuts at: 5 52 2191 4366 5 Size: 47 2139 2175 2081 Fragments arranged by size: 2175 2139 2081 47 StuI AGG'CCT Cuts at: 1517 3647 1517 Size: 2130 4312 StyI C'CwwG_G Cuts at: 513 1065 2004 3881 513 Size: 552 939 1877 3074 Fragments arranged by size: 3074 1877 939 552 SwaI ATTT'AAAT Cuts at: 2892 2892 Size: 6442 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4469 4486 4639 4824 5269 325 Size: 1255 2889 17 153 185 445 1498 Fragments arranged by size: 2889 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2412 4688 6400 Size: 274 80 33 51 1443 435 2276 1712 Cuts at: 6400 96 Size: 138 Fragments arranged by size: 2276 1712 1443 435 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6402 6434 6402 Size: 32 6410 Tth111I GACn'n_nGTC Cuts at: 1316 3305 1316 Size: 1989 4453 XbaI T'CTAG_A Cuts at: 3778 3778 Size: 6442 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2698 2698 Size: 6442 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6442 XmaI C'CCGG_G Cuts at: 1870 3767 1870 Size: 1897 4545 XmnI GAAnn'nnTTC Cuts at: 2099 2862 4571 2099 Size: 763 1709 3970 Fragments arranged by size: 3970 1709 763 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4250 276 Size: 53 83 186 936 2716 2468 Fragments arranged by size: 2716 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI