(Circular) (Six-base) MAPSORT of: pFN21AB9055.seq Check: 4624 from: 1 to: 7639 pFN21AB9055 7639 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5449 278 Size: 53 83 186 936 3913 2468 Fragments arranged by size: 3913 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4982 1937 Size: 3045 4594 Acc65I G'GTAC_C Cuts at: 2404 4960 2404 Size: 2556 5083 AclI AA'CG_TT Cuts at: 1563 4077 5766 6139 1563 Size: 2514 1689 373 3063 Fragments arranged by size: 3063 2514 1689 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2766 2829 2886 3174 3285 3420 3612 5702 Size: 1134 63 57 288 111 135 192 2090 Cuts at: 5702 7086 7354 1632 Size: 1384 268 1917 Fragments arranged by size: 2090 1917 1384 1134 288 268 192 135 111 63 57 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7442 AflIII A'CryG_T Cuts at: 1203 2163 2836 3269 6538 7515 1203 Size: 960 673 433 3269 977 1327 Fragments arranged by size: 3269 1327 977 960 673 433 AgeI A'CCGG_T Cuts at: 6470 6470 Size: 7639 AhdI GACnn_n'nnGTC Cuts at: 6368 6368 Size: 7639 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7639 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7639 AlwNI CAG_nnn'CTG Cuts at: 2615 5382 6954 2615 Size: 2767 1572 3300 Fragments arranged by size: 3300 2767 1572 ApaLI G'TGCA_C Cuts at: 5696 6852 5696 Size: 1156 6483 ApoI r'AATT_y Cuts at: 1493 3159 3189 3262 3374 3425 3548 3689 3819 Size: 1666 30 73 112 51 123 141 130 Cuts at: 3819 4429 4580 4754 4948 5174 5326 7435 1493 Size: 610 151 174 194 226 152 2109 1697 Fragments arranged by size: 2109 1697 1666 610 226 194 174 152 151 141 130 123 112 73 51 30 AseI AT'TA_AT Cuts at: 160 1032 4791 6193 160 Size: 872 3759 1402 1606 Fragments arranged by size: 3759 1606 1402 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7639 AvaI C'yCGr_G Cuts at: 1870 1943 2093 2489 4675 4964 1870 Size: 73 150 396 2186 289 4545 Fragments arranged by size: 4545 2186 396 289 150 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2388 2421 2388 Size: 33 7606 BamHI G'GATC_C Cuts at: 4969 5336 4969 Size: 367 7272 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2404 4213 4960 6415 618 Size: 325 217 627 617 1809 747 1455 1842 Fragments arranged by size: 1842 1809 1455 747 627 617 325 217 BanII G_rGCy'C Cuts at: 1391 4415 4958 1391 Size: 3024 543 4072 Fragments arranged by size: 4072 3024 543 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4251 4294 4476 928 Size: 971 2352 43 182 4091 Fragments arranged by size: 4091 2352 971 182 43 BbvCI CC'TCA_GC Cuts at: 4039 4039 Size: 7639 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5830 5864 1903 Size: 34 3893 34 3678 Fragments arranged by size: 3893 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5531 6741 5531 Size: 1210 6429 BclI T'GATC_A Cuts at: 1548 4807 1548 Size: 3259 4380 BfrBI ATG'CAT Cuts at: 2275 2275 Size: 7639 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2135 6250 136 Size: 107 122 71 631 605 463 4115 1525 Fragments arranged by size: 4115 1525 631 605 463 122 107 71 BglII A'GATC_T Cuts at: 3130 7634 3130 Size: 4504 3135 BlpI GC'TnA_GC Cuts at: 5056 5056 Size: 7639 Bme1580I G_kGCm'C Cuts at: 1790 5700 6856 1790 Size: 3910 1156 2573 Fragments arranged by size: 3910 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2164 2192 1318 Size: 846 28 6765 Fragments arranged by size: 6765 846 28 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5370 6328 442 Size: 416 53 83 399 24 3953 958 1753 Fragments arranged by size: 3953 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7639 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2068 4417 2068 Size: 2349 5290 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2619 3221 4337 6299 1239 Size: 1380 602 1116 1962 2579 Fragments arranged by size: 2579 1962 1380 1116 602 Bpu10I CC'TnA_GC Cuts at: 4039 4039 Size: 7639 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3051 3681 5764 6629 6927 3051 Size: 630 2083 865 298 3763 Fragments arranged by size: 3763 2083 865 630 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2427 2554 2908 6302 882 Size: 617 928 127 354 3394 2219 Fragments arranged by size: 3394 2219 928 617 354 127 BsaAI yAC'GTr Cuts at: 493 1204 3029 3272 3454 4935 5430 493 Size: 711 1825 243 182 1481 495 2702 Fragments arranged by size: 2702 1825 1481 711 495 243 182 BsaBI GATnn'nnATC Cuts at: 2748 7633 2748 Size: 4885 2754 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5446 5828 6463 275 Size: 53 83 186 936 3913 382 635 1451 Fragments arranged by size: 3913 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2464 3088 6072 6470 6744 6891 7302 2464 Size: 624 2984 398 274 147 411 2801 Fragments arranged by size: 2984 2801 624 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3749 3779 1178 Size: 30 165 30 2346 30 5038 Fragments arranged by size: 5038 2346 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 4393 1186 Size: 874 2333 4432 Fragments arranged by size: 4432 2333 874 BseYI C'CCAG_C Cuts at: 2966 4697 6842 7338 2966 Size: 1731 2145 496 3267 Fragments arranged by size: 3267 2145 1731 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2474 1163 Size: 573 738 6328 Fragments arranged by size: 6328 738 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5089 5850 6878 1540 Size: 275 186 3088 761 1028 2301 Fragments arranged by size: 3088 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3398 4400 5700 5785 6856 3398 Size: 1002 1300 85 1071 4181 Fragments arranged by size: 4181 1300 1071 1002 85 BsmI GAATG_Cn' Cuts at: 3013 4129 4305 5150 5243 3013 Size: 1116 176 845 93 5409 Fragments arranged by size: 5409 1116 845 176 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4373 4373 Size: 7639 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3398 4400 4415 4958 5700 5785 6856 Size: 399 1608 1002 15 543 742 85 1071 Cuts at: 6856 1391 Size: 2174 Fragments arranged by size: 2174 1608 1071 1002 742 543 399 85 15 BspEI T'CCGG_A Cuts at: 2464 7302 2464 Size: 4838 2801 BspHI T'CATG_A Cuts at: 5528 7258 5528 Size: 1730 5909 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4994 844 Size: 4150 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5526 1394 Size: 429 3703 3507 Fragments arranged by size: 3703 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2025 2142 2797 6134 6308 65 Size: 1183 777 117 655 3337 174 1396 Fragments arranged by size: 3337 1396 1183 777 655 174 117 BsrFI r'CCGG_y Cuts at: 1700 1810 6283 6470 1700 Size: 110 4473 187 2869 Fragments arranged by size: 4473 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7639 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7639 BssSI C'ACGA_G Cuts at: 4092 5699 6711 4092 Size: 1607 1012 5020 Fragments arranged by size: 5020 1607 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7639 BstBI TT'CG_AA Cuts at: 4048 6443 4048 Size: 2395 5244 BstEII G'GTnAC_C Cuts at: 1184 4000 1184 Size: 2816 4823 BstXI CCAn_nnnn'nTGG Cuts at: 4705 4705 Size: 7639 BstYI r'GATC_y Cuts at: 1972 2737 3130 4728 4969 5336 5721 5738 7190 Size: 765 393 1598 241 367 385 17 1452 Cuts at: 7190 7276 7288 7634 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1598 1452 765 393 385 367 346 241 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4269 4269 Size: 7639 BtgI C'CryG_G Cuts at: 513 1065 2004 3844 4005 513 Size: 552 939 1840 161 4147 Fragments arranged by size: 4147 1840 939 552 161 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6900 Fragments arranged by size: 6900 683 56 BtsI GCAGTG_nn' Cuts at: 2154 5155 5949 5969 2154 Size: 3001 794 20 3824 Fragments arranged by size: 3824 3001 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7639 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7604 DraI TTT'AAA Cuts at: 2482 4943 2482 Size: 2461 5178 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2168 3963 6646 809 Size: 507 852 1795 2683 1802 Fragments arranged by size: 2683 1802 1795 852 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2115 5086 5975 Size: 54 1425 50 275 18 285 2971 889 Cuts at: 5975 7604 8 Size: 1629 43 Fragments arranged by size: 2971 1629 1425 889 285 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5086 1537 Size: 275 3274 4090 Fragments arranged by size: 4090 3274 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3666 4169 5569 1353 Size: 2313 503 1400 3423 Fragments arranged by size: 3423 2313 1400 503 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6226 6600 6746 387 Size: 1064 138 4637 374 146 1280 Fragments arranged by size: 4637 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2619 2766 2829 2886 3174 3221 3285 Size: 393 987 147 63 57 288 47 64 Cuts at: 3285 3420 3612 4337 5702 6299 7086 7354 1239 Size: 135 192 725 1365 597 787 268 1524 Fragments arranged by size: 1524 1365 987 787 725 597 393 288 268 192 147 135 64 63 57 47 EcoO109I rG'GnC_Cy Cuts at: 2860 4013 4826 2860 Size: 1153 813 5673 Fragments arranged by size: 5673 1153 813 EcoRI G'AATT_C Cuts at: 3159 3189 3262 3819 4948 5326 3159 Size: 30 73 557 1129 378 5472 Fragments arranged by size: 5472 1129 557 378 73 30 FspI TGC'GCA Cuts at: 6145 6145 Size: 7639 HaeII r_GCGC'y Cuts at: 1678 6786 1678 Size: 5108 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2033 2065 3340 3372 4889 Size: 32 135 32 461 32 1275 32 1517 Cuts at: 4889 4921 6280 6312 6354 6386 1373 Size: 32 1359 32 42 32 2626 Fragments arranged by size: 2626 1517 1359 1275 461 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4983 5229 669 Size: 988 281 3045 246 3079 Fragments arranged by size: 3079 3045 988 281 246 HindIII A'AGCT_T Cuts at: 748 2382 3936 748 Size: 1634 1554 4451 Fragments arranged by size: 4451 1634 1554 HpaI GTT'AAC Cuts at: 5229 5229 Size: 7639 KpnI G_GTAC'C Cuts at: 2408 4964 2408 Size: 2556 5083 MfeI C'AATT_G Cuts at: 5238 5238 Size: 7639 MluI A'CGCG_T Cuts at: 7515 7515 Size: 7639 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2155 4556 4842 6753 6937 1305 Size: 850 2401 286 1911 184 2007 Fragments arranged by size: 2401 2007 1911 850 286 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2117 10 Size: 54 1425 343 285 5532 Fragments arranged by size: 5532 1425 343 285 54 MslI CAynn'nnrTG Cuts at: 518 1166 2147 2589 2660 2804 3441 3530 4487 Size: 648 981 442 71 144 637 89 957 Cuts at: 4487 5597 5956 6115 518 Size: 1110 359 159 2042 Fragments arranged by size: 2042 1110 981 957 648 637 442 359 159 144 89 71 MspA1I CmG'CkG Cuts at: 1606 2711 3846 4043 4801 4847 5055 5732 6880 Size: 1105 1135 197 758 46 208 677 1148 Cuts at: 6880 7125 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1135 1105 758 677 245 208 197 46 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6148 Fragments arranged by size: 6148 939 552 NdeI CA'TA_TG Cuts at: 387 2416 387 Size: 2029 5610 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7639 NotI GC'GGCC_GC Cuts at: 5086 5086 Size: 7639 NsiI A_TGCA'T Cuts at: 2277 2277 Size: 7639 NspI r_CATG'y Cuts at: 1132 1229 2588 2840 4672 4997 6542 1132 Size: 97 1359 252 1832 325 1545 2229 Fragments arranged by size: 2229 1832 1545 1359 325 252 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7639 PciI A'CATG_T Cuts at: 2836 6538 2836 Size: 3702 3937 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7639 PfoI T'CCnGG_A Cuts at: 2949 4239 4777 2949 Size: 1290 538 5811 Fragments arranged by size: 5811 1290 538 PmeI GTTT'AAAC Cuts at: 4943 4943 Size: 7639 PmlI CAC'GTG Cuts at: 3272 4935 3272 Size: 1663 5976 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5708 5740 7221 7253 5708 Size: 32 1481 32 6094 Fragments arranged by size: 6094 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2860 2860 Size: 7639 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7639 PsiI TTA'TAA Cuts at: 5209 5209 Size: 7639 PstI C_TGCA'G Cuts at: 830 2269 4991 830 Size: 1439 2722 3478 Fragments arranged by size: 3478 2722 1439 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7639 PvuII CAG'CTG Cuts at: 2711 4043 4801 4847 2711 Size: 1332 758 46 5503 Fragments arranged by size: 5503 1332 758 46 SacII CC_GC'GG Cuts at: 3847 3847 Size: 7639 SalI G'TCGA_C Cuts at: 1936 4981 1936 Size: 3045 4594 SbfI CC_TGCA'GG Cuts at: 4991 4991 Size: 7639 ScaI AGT'ACT Cuts at: 5887 7599 5887 Size: 1712 5927 SexAI A'CCwGG_T Cuts at: 3809 3809 Size: 7639 SfcI C'TryA_G Cuts at: 826 1045 2265 2322 4624 4987 6122 6803 6994 Size: 219 1220 57 2302 363 1135 681 191 Cuts at: 6994 826 Size: 1471 Fragments arranged by size: 2302 1471 1220 1135 681 363 219 191 57 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7639 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7639 SmaI CCC'GGG Cuts at: 1872 4677 4966 1872 Size: 2805 289 4545 Fragments arranged by size: 4545 2805 289 SmlI C'TyrA_G Cuts at: 820 1017 1943 2093 3066 3696 5743 6644 6906 Size: 197 926 150 973 630 2047 901 262 Cuts at: 6906 820 Size: 1553 Fragments arranged by size: 2047 1553 973 926 901 630 262 197 150 SnaBI TAC'GTA Cuts at: 493 3029 5430 493 Size: 2536 2401 2702 Fragments arranged by size: 2702 2536 2401 SpeI A'CTAG_T Cuts at: 152 3886 152 Size: 3734 3905 SphI G_CATG'C Cuts at: 1132 4672 4997 1132 Size: 3540 325 3774 Fragments arranged by size: 3774 3540 325 SspI AAT'ATT Cuts at: 5 52 2240 5563 5 Size: 47 2188 3323 2081 Fragments arranged by size: 3323 2188 2081 47 StuI AGG'CCT Cuts at: 1517 4118 1517 Size: 2601 5038 StyI C'CwwG_G Cuts at: 513 1065 2004 3387 3405 3509 5078 513 Size: 552 939 1383 18 104 1569 3074 Fragments arranged by size: 3074 1569 1383 939 552 104 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4453 5666 5683 5836 6021 6466 325 Size: 1255 2873 1213 17 153 185 445 1498 Fragments arranged by size: 2873 1498 1255 1213 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5885 7597 96 Size: 274 80 33 51 1443 3908 1712 138 Fragments arranged by size: 3908 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4570 4602 7599 7631 4570 Size: 32 2997 32 4578 Fragments arranged by size: 4578 2997 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7639 XbaI T'CTAG_A Cuts at: 4975 4975 Size: 7639 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2131 4932 2131 Size: 2801 4838 XhoI C'TCGA_G Cuts at: 1943 2093 1943 Size: 150 7489 XmaI C'CCGG_G Cuts at: 1870 4675 4964 1870 Size: 2805 289 4545 Fragments arranged by size: 4545 2805 289 XmnI GAAnn'nnTTC Cuts at: 3147 3159 3228 3474 5768 3147 Size: 12 69 246 2294 5018 Fragments arranged by size: 5018 2294 246 69 12 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5447 276 Size: 53 83 186 936 3913 2468 Fragments arranged by size: 3913 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI