Restriction map for
pFN21AB9027
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BanI BanII BbeI
BbrPI BbsI BbvCI BbvI BcgI
BciVI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaHI BsaI BseAI BseRI BsiEI
BsiWI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstZ17I CelII
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV EcoT14I
EcoT22I FauI FokI FseI FspI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeII MfeI MluI MluNI MroI
MscI MslI MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PleI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TseI Tsp45I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccII 52 CGCG CG^CG
AcsI 590 GAATTC [GA]^AATT[CT]
AgeI 42 ACCGGT A^CCGGT
AocI 664 CCTGAGG CC^T[ACGT]AGG
ApoI 590 GAATTC [GA]^AATT[CT]
Asp700I 423 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 469 GTGCTC G[AT]GC[AT]^C
AvaII 513 GGACC G^G[AT]CC
BamHI 642 GGATCC G^GATCC
BclI 104 TGATCA T^GATCA
BfaI 253 CTAG C^TAG
BmyI 469 GTGCTC G[AGT]GC[ACT]^C
BpmI 22 CTGGAG CTGGAG(16/14)
BsaBI 637 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 42 ACCGGT [AT]^CCGG[AT]
BsgI 91 GTGCAG GTGCAG(16/14)
BsiHKAI 469 GTGCTC G[AT]GC[AT]^C
BsmI 248 GAATGC GAATGC(1/-1)
Bsp1286I 469 GTGCTC G[AGT]GC[ACT]^C
BspMI 29 ACCTGC ACCTGC(4/8)
BsrFI 42 ACCGGT [GA]^CCGG[CT]
BsrI 196 CCAGT ACTGG(1/-1)
BstUI 52 CGCG CG^CG
BstXI 702 CCAGCGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 664 CCTGAGG CC^T[ACGT]AGG
CfoI 53 GCGC GCG^C
Cfr10I 42 ACCGGT [GA]^CCGG[CT]
DraI 145 TTTAAA TTT^AAA
DraII 449 AGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 673 CGGCCA [CT]^GGCC[GA]
Eco81I 664 CCTGAGG CC^T[ACGT]AGG
EcoO109I 449 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 590 GAATTC G^AATTC
FbaI 104 TGATCA T^GATCA
HapII 43 CCGG C^CGG
HgaI 71 GCGTC GACGC(5/10)
HhaI 53 GCGC GCG^C
HinP1I 53 GCGC G^CGC
HpaII 43 CCGG C^CGG
MaeI 253 CTAG C^TAG
MaeIII 239 GTTAC ^GT[ACGT]AC
MamI 637 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 703 CAGCGG C[AC]G^C[GT]G
MspI 43 CCGG C^CGG
MvnI 52 CGCG CG^CG
MwoI 462 GCCCTGGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PinAI 42 ACCGGT A^CCGGT
PstI 31 CTGCAG CTGCA^G
SexAI 576 ACCTGGT A^CC[AT]GGT
SfcI 31 CTGCAG C^T[GA][CT]AG
StuI 365 AGGCCT AGG^CCT
TaqI 246 TCGA T^CGA
TspRI 63 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 246 TCGA T^CGA
XmnI 423 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 9 enzymes) #
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AseI 138 ATTAAT AT^TAAT
168 ATTAAT
AsnI 138 ATTAAT AT^TAAT
168 ATTAAT
BstYI 35 AGATCC [GA]^GATC[CT]
642 GGATCC
DdeI 348 CTTAG C^T[ACGT]AG
665 CTGAG
HinfI 203 GATTC G^A[ACGT]TC
314 GATTC
MflI 35 AGATCC [GA]^GATC[CT]
642 GGATCC
PshBI 138 ATTAAT AT^TAAT
168 ATTAAT
TfiI 203 GATTC G^A[AT]TC
314 GATTC
XhoII 35 AGATCC [GA]^GATC[CT]
642 GGATCC
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AfaI AluI AlwI BsaJI
BssKI BstNI Cac8I Cfr13I DpnII
EcoRII Fnu4HI HaeIII HphI ItaI
MboI MboII MnlI MseI MvaI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI Tru9I Tsp509I