(Circular) (Six-base) MAPSORT of: pFN21AB9023.seq Check: 2719 from: 1 to: 8830 pFN21AB9023 8830 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3818 4555 3818 Size: 737 8093 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6640 278 Size: 53 83 186 936 5104 2468 Fragments arranged by size: 5104 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3201 5257 6102 6173 1937 Size: 1264 2056 845 71 4594 Fragments arranged by size: 4594 2056 1264 845 71 Acc65I G'GTAC_C Cuts at: 4377 4982 6151 4377 Size: 605 1169 7056 Fragments arranged by size: 7056 1169 605 AclI AA'CG_TT Cuts at: 1563 6957 7330 1563 Size: 5394 373 3063 Fragments arranged by size: 5394 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2645 2729 2887 3342 3345 3600 3802 5372 Size: 1013 84 158 455 3 255 202 1570 Cuts at: 5372 5589 5944 6893 8277 8545 1632 Size: 217 355 949 1384 268 1917 Fragments arranged by size: 1917 1570 1384 1013 949 455 355 268 255 217 202 158 84 3 AfeI AGC'GCT Cuts at: 5066 5066 Size: 8830 AflII C'TTAA_G Cuts at: 820 1017 3376 820 Size: 197 2359 6274 Fragments arranged by size: 6274 2359 197 AflIII A'CryG_T Cuts at: 1203 4308 7729 8706 1203 Size: 3105 3421 977 1327 Fragments arranged by size: 3421 3105 1327 977 AgeI A'CCGG_T Cuts at: 7661 7661 Size: 8830 AhdI GACnn_n'nnGTC Cuts at: 5930 7559 5930 Size: 1629 7201 AleI CACnn'nnGTG Cuts at: 1166 3656 4280 4311 5094 1166 Size: 2490 624 31 783 4902 Fragments arranged by size: 4902 2490 783 624 31 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4738 4857 4928 5231 5348 1184 Size: 3554 119 71 303 117 4666 Fragments arranged by size: 4666 3554 303 119 117 71 AlwNI CAG_nnn'CTG Cuts at: 2146 3833 4174 5534 6573 8145 2146 Size: 1687 341 1360 1039 1572 2831 Fragments arranged by size: 2831 1687 1572 1360 1039 341 ApaI G_GGCC'C Cuts at: 3134 4873 3134 Size: 1739 7091 ApaLI G'TGCA_C Cuts at: 3644 4672 6887 8043 3644 Size: 1028 2215 1156 4431 Fragments arranged by size: 4431 2215 1156 1028 ApoI r'AATT_y Cuts at: 1493 3976 5548 5802 6139 6365 6517 8626 1493 Size: 2483 1572 254 337 226 152 2109 1697 Fragments arranged by size: 2483 2109 1697 1572 337 254 226 152 AseI AT'TA_AT Cuts at: 160 1032 7384 160 Size: 872 6352 1606 Fragments arranged by size: 6352 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8830 AvaI C'yCGr_G Cuts at: 1870 1943 2530 2673 3868 5106 6155 1870 Size: 73 587 143 1195 1238 1049 4545 Fragments arranged by size: 4545 1238 1195 1049 587 143 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3227 3260 3227 Size: 33 8797 BamHI G'GATC_C Cuts at: 2787 3088 5713 6160 6527 2787 Size: 301 2625 447 367 5090 Fragments arranged by size: 5090 2625 447 367 301 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2237 3958 4329 4377 4665 Size: 325 217 627 450 1721 371 48 288 Cuts at: 4665 4982 5099 5222 5240 5324 5378 6151 7606 Size: 317 117 123 18 84 54 773 1455 Cuts at: 7606 618 Size: 1842 Fragments arranged by size: 1842 1721 1455 773 627 450 371 325 317 288 217 123 117 84 54 48 18 BanII G_rGCy'C Cuts at: 1391 2112 3134 3159 4873 5037 6149 1391 Size: 721 1022 25 1714 164 1112 4072 Fragments arranged by size: 4072 1714 1112 1022 721 164 25 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3414 3427 928 Size: 971 1515 13 6331 Fragments arranged by size: 6331 1515 971 13 BbvCI CC'TCA_GC Cuts at: 3903 3903 Size: 8830 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2179 2213 7021 7055 1903 Size: 34 242 34 4808 34 3678 Fragments arranged by size: 4808 3678 242 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3049 6722 7932 3049 Size: 3673 1210 3947 Fragments arranged by size: 3947 3673 1210 BclI T'GATC_A Cuts at: 1548 4536 5808 1548 Size: 2988 1272 4570 Fragments arranged by size: 4570 2988 1272 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2474 3704 3774 Size: 107 122 71 631 605 802 1230 70 Cuts at: 3774 7441 136 Size: 3667 1525 Fragments arranged by size: 3667 1525 1230 802 631 605 122 107 71 70 BglII A'GATC_T Cuts at: 5683 8825 5683 Size: 3142 5688 BlpI GC'TnA_GC Cuts at: 2170 6247 2170 Size: 4077 4753 Bme1580I G_kGCm'C Cuts at: 1790 2240 3134 3648 4668 4676 4873 5127 5225 Size: 450 894 514 1020 8 197 254 98 Cuts at: 5225 5327 6891 8047 1790 Size: 102 1564 1156 2573 Fragments arranged by size: 2573 1564 1156 1020 894 514 450 254 197 102 98 8 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8830 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4846 5122 5531 Size: 416 53 83 399 24 3429 276 409 Cuts at: 5531 6561 7519 442 Size: 1030 958 1753 Fragments arranged by size: 3429 1753 1030 958 416 409 399 276 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8830 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2404 2404 Size: 8830 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2814 5089 5105 5918 6022 7490 1239 Size: 1575 2275 16 813 104 1468 2579 Fragments arranged by size: 2579 2275 1575 1468 813 104 16 Bpu10I CC'TnA_GC Cuts at: 3903 3929 3903 Size: 26 8804 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4792 5933 6955 7820 8118 4792 Size: 1141 1022 865 298 5504 Fragments arranged by size: 5504 1141 1022 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2853 3167 7493 882 Size: 617 1354 314 4326 2219 Fragments arranged by size: 4326 2219 1354 617 314 BsaAI yAC'GTr Cuts at: 493 1204 2233 3441 3628 5122 6621 493 Size: 711 1029 1208 187 1494 1499 2702 Fragments arranged by size: 2702 1499 1494 1208 1029 711 187 BsaBI GATnn'nnATC Cuts at: 6057 8824 6057 Size: 2767 6063 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6637 7019 7654 275 Size: 53 83 186 936 5104 382 635 1451 Fragments arranged by size: 5104 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4279 7263 7661 7935 8082 8493 4279 Size: 2984 398 274 147 411 4616 Fragments arranged by size: 4616 2984 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3273 3303 4004 4010 4034 Size: 30 165 30 1870 30 701 6 24 Cuts at: 4034 4040 4567 4597 4975 5005 5254 5284 5998 Size: 6 527 30 378 30 249 30 714 Cuts at: 5998 6028 1178 Size: 30 3980 Fragments arranged by size: 3980 1870 714 701 527 378 249 165 30 30 30 30 30 30 30 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2519 2984 3017 3734 3890 6019 1186 Size: 1333 465 33 717 156 2129 3997 Fragments arranged by size: 3997 2129 1333 717 465 156 33 BseYI C'CCAG_C Cuts at: 2401 3184 3313 3970 4167 4589 5232 5871 8033 Size: 783 129 657 197 422 643 639 2162 Cuts at: 8033 8529 2401 Size: 496 2702 Fragments arranged by size: 2702 2162 783 657 643 639 496 422 197 129 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2081 2183 3490 4150 5237 1163 Size: 573 345 102 1307 660 1087 4756 Fragments arranged by size: 4756 1307 1087 660 573 345 102 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2033 5544 6280 7041 8069 1540 Size: 275 186 32 3511 736 761 1028 2301 Fragments arranged by size: 3511 2301 1028 761 736 275 186 32 BsiHKAI G_wGCw'C Cuts at: 2112 3648 4585 4676 5091 6891 6976 8047 2112 Size: 1536 937 91 415 1800 85 1071 2895 Fragments arranged by size: 2895 1800 1536 1071 937 415 91 85 BsmI GAATG_Cn' Cuts at: 4694 6341 6434 4694 Size: 1647 93 7090 Fragments arranged by size: 7090 1647 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3872 5531 3872 Size: 1659 7171 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2112 2240 3134 3159 3648 4585 4668 Size: 399 322 128 894 25 489 937 83 Cuts at: 4668 4676 4873 5037 5091 5127 5225 5327 6149 Size: 8 197 164 54 36 98 102 822 Cuts at: 6149 6891 6976 8047 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 937 894 822 742 489 399 322 197 164 128 102 98 85 83 54 36 25 8 BspEI T'CCGG_A Cuts at: 8493 8493 Size: 8830 BspHI T'CATG_A Cuts at: 4969 6719 8449 4969 Size: 1750 1730 5350 Fragments arranged by size: 5350 1750 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2479 3672 3818 4555 4730 5088 6185 844 Size: 1635 1193 146 737 175 358 1097 3489 Fragments arranged by size: 3489 1635 1193 1097 737 358 175 146 BsrBI CCG'CTC Cuts at: 1394 1823 5229 6717 1394 Size: 429 3406 1488 3507 Fragments arranged by size: 3507 3406 1488 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3691 7325 7499 65 Size: 1183 2443 3634 174 1396 Fragments arranged by size: 3634 2443 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2032 4518 4655 7474 7661 1700 Size: 110 222 2486 137 2819 187 2869 Fragments arranged by size: 2869 2819 2486 222 187 137 110 BsrGI T'GTAC_A Cuts at: 96 5903 96 Size: 5807 3023 BssHII G'CGCG_C Cuts at: 1925 2425 2447 1925 Size: 500 22 8308 Fragments arranged by size: 8308 500 22 BssSI C'ACGA_G Cuts at: 2347 6890 7902 2347 Size: 4543 1012 3275 Fragments arranged by size: 4543 3275 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3574 3833 794 Size: 2780 259 5791 Fragments arranged by size: 5791 2780 259 BstBI TT'CG_AA Cuts at: 3343 7634 3343 Size: 4291 4539 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8830 BstXI CCAn_nnnn'nTGG Cuts at: 4388 5357 5437 5710 5896 4388 Size: 969 80 273 186 7322 Fragments arranged by size: 7322 969 273 186 80 BstYI r'GATC_y Cuts at: 1972 2787 3088 5683 5713 6160 6527 6912 6929 Size: 815 301 2595 30 447 367 385 17 Cuts at: 6929 8381 8467 8479 8825 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2595 1977 1452 815 447 385 367 346 301 86 30 17 12 BstZ17I GTA'TAC Cuts at: 5258 5258 Size: 8830 BtgI C'CryG_G Cuts at: 513 1065 2004 2088 2175 2353 4612 5167 5277 Size: 552 939 84 87 178 2259 555 110 Cuts at: 5277 513 Size: 4066 Fragments arranged by size: 4066 2259 939 555 552 178 110 87 84 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2305 4418 4674 5148 5689 487 Size: 683 56 1079 2113 256 474 541 3628 Fragments arranged by size: 3628 2113 1079 683 541 474 256 56 BtsI GCAGTG_nn' Cuts at: 2403 3535 5168 6346 7140 7160 2403 Size: 1132 1633 1178 794 20 4073 Fragments arranged by size: 4073 1633 1178 1132 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8830 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8795 DraI TTT'AAA Cuts at: 3141 6134 3141 Size: 2993 5837 DraIII CAC_nnn'GTG Cuts at: 3598 4823 3598 Size: 1225 7605 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5413 7837 809 Size: 507 4097 2424 1802 Fragments arranged by size: 4097 2424 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 2030 2316 Size: 54 1425 50 275 18 192 8 286 Cuts at: 2316 2409 2475 3016 3616 3705 5297 6040 6277 Size: 93 66 541 600 89 1592 743 237 Cuts at: 6277 7166 8795 8 Size: 889 1629 43 Fragments arranged by size: 1629 1592 1425 889 743 600 541 286 275 237 192 93 89 66 54 50 43 18 8 EagI C'GGCC_G Cuts at: 1537 1812 2030 6277 1537 Size: 275 218 4247 4090 Fragments arranged by size: 4247 4090 275 218 EarI CTCTTCn'nnn_ Cuts at: 1353 2117 2884 5678 6760 1353 Size: 764 767 2794 1082 3423 Fragments arranged by size: 3423 2794 1082 767 764 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2351 7417 7791 7937 387 Size: 1064 138 762 5066 374 146 1280 Fragments arranged by size: 5066 1280 1064 762 374 146 138 EcoICRI GAG'CTC Cuts at: 2110 2110 Size: 8830 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2645 2729 2814 2887 3342 3345 3600 Size: 393 1013 84 85 73 455 3 255 Cuts at: 3600 3802 5089 5105 5372 5589 5918 5944 6022 Size: 202 1287 16 267 217 329 26 78 Cuts at: 6022 6893 7490 8277 8545 1239 Size: 871 597 787 268 1524 Fragments arranged by size: 1524 1287 1013 871 787 597 455 393 329 268 267 255 217 202 85 84 78 73 26 16 3 EcoO109I rG'GnC_Cy Cuts at: 3130 3739 4869 5046 5668 3130 Size: 609 1130 177 622 6292 Fragments arranged by size: 6292 1130 622 609 177 EcoRI G'AATT_C Cuts at: 5548 5802 6139 6517 5548 Size: 254 337 378 7861 Fragments arranged by size: 7861 378 337 254 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5197 5689 5974 5197 Size: 492 285 8053 Fragments arranged by size: 8053 492 285 FspI TGC'GCA Cuts at: 4369 7336 4369 Size: 2967 5863 FspAI rTGC'GCAy Cuts at: 4369 4369 Size: 8830 HaeII r_GCGC'y Cuts at: 1678 2096 2499 2525 4840 5068 5643 7977 1678 Size: 418 403 26 2315 228 575 2334 2531 Fragments arranged by size: 2531 2334 2315 575 418 403 228 26 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2153 2185 2352 2384 2696 Size: 32 135 32 581 32 167 32 312 Cuts at: 2696 2728 4355 4387 4676 4708 4790 4822 5484 Size: 32 1627 32 289 32 82 32 662 Cuts at: 5484 5516 5840 5872 5916 5948 6066 6098 7471 Size: 32 324 32 44 32 118 32 1373 Cuts at: 7471 7503 7545 7577 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1627 1373 662 581 324 312 289 167 135 118 82 44 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4760 6174 6420 669 Size: 988 281 2822 1414 246 3079 Fragments arranged by size: 3079 2822 1414 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8830 HpaI GTT'AAC Cuts at: 6420 6420 Size: 8830 KpnI G_GTAC'C Cuts at: 4381 4986 6155 4381 Size: 605 1169 7056 Fragments arranged by size: 7056 1169 605 MfeI C'AATT_G Cuts at: 3759 6429 3759 Size: 2670 6160 MluI A'CGCG_T Cuts at: 8706 8706 Size: 8830 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2811 2836 3447 4047 7944 8128 1305 Size: 1506 25 611 600 3897 184 2007 Fragments arranged by size: 3897 2007 1506 611 600 184 25 MscI TGG'CCA Cuts at: 10 64 1489 1832 2477 3018 3618 6042 10 Size: 54 1425 343 645 541 600 2424 2798 Fragments arranged by size: 2798 2424 1425 645 600 541 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3656 4280 4311 4437 4578 5094 5435 Size: 648 2490 624 31 126 141 516 341 Cuts at: 5435 5708 6788 7147 7306 518 Size: 273 1080 359 159 2042 Fragments arranged by size: 2490 2042 1080 648 624 516 359 341 273 159 141 126 31 MspA1I CmG'CkG Cuts at: 1606 2090 2849 2870 4174 4632 6246 6923 8071 Size: 484 759 21 1304 458 1614 677 1148 Cuts at: 8071 8316 1606 Size: 245 2120 Fragments arranged by size: 2120 1614 1304 1148 759 677 484 458 245 21 NaeI GCC'GGC Cuts at: 4657 4657 Size: 8830 NcoI C'CATG_G Cuts at: 513 1065 2004 4612 5277 513 Size: 552 939 2608 665 4066 Fragments arranged by size: 4066 2608 939 665 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8830 NgoMIV G'CCGG_C Cuts at: 4655 4655 Size: 8830 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8830 NotI GC'GGCC_GC Cuts at: 6277 6277 Size: 8830 NspI r_CATG'y Cuts at: 1132 1229 3500 3671 4312 6188 7733 1132 Size: 97 2271 171 641 1876 1545 2229 Fragments arranged by size: 2271 2229 1876 1545 641 171 97 PasI CC'CwG_GG Cuts at: 1348 2271 2890 3159 4865 1348 Size: 923 619 269 1706 5313 Fragments arranged by size: 5313 1706 923 619 269 PciI A'CATG_T Cuts at: 4308 7729 4308 Size: 3421 5409 PflMI CCAn_nnn'nTGG Cuts at: 1108 3850 1108 Size: 2742 6088 PfoI T'CCnGG_A Cuts at: 5622 5622 Size: 8830 PmeI GTTT'AAAC Cuts at: 6134 6134 Size: 8830 PmlI CAC'GTG Cuts at: 3628 3628 Size: 8830 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6899 6931 8412 8444 6899 Size: 32 1481 32 7285 Fragments arranged by size: 7285 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 5668 5668 Size: 8830 PshAI GACnn'nnGTC Cuts at: 1613 4150 1613 Size: 2537 6293 PsiI TTA'TAA Cuts at: 6400 6400 Size: 8830 PspOMI G'GGCC_C Cuts at: 3130 4869 3130 Size: 1739 7091 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4103 4135 4103 Size: 32 8798 PstI C_TGCA'G Cuts at: 830 2070 2151 2619 2854 4717 5163 6100 6182 Size: 1240 81 468 235 1863 446 937 82 Cuts at: 6182 830 Size: 3478 Fragments arranged by size: 3478 1863 1240 937 468 446 235 82 81 PvuI CG_AT'CG Cuts at: 2001 5544 2001 Size: 3543 5287 PvuII CAG'CTG Cuts at: 2849 4174 4632 2849 Size: 1325 458 7047 Fragments arranged by size: 7047 1325 458 SacI G_AGCT'C Cuts at: 2112 2112 Size: 8830 SacII CC_GC'GG Cuts at: 2091 2091 Size: 8830 SalI G'TCGA_C Cuts at: 1936 6172 1936 Size: 4236 4594 SapI GCTCTTCn'nnn_ Cuts at: 2117 2117 Size: 8830 SbfI CC_TGCA'GG Cuts at: 2151 6182 2151 Size: 4031 4799 ScaI AGT'ACT Cuts at: 7078 8790 7078 Size: 1712 7118 SexAI A'CCwGG_T Cuts at: 4122 4122 Size: 8830 SfcI C'TryA_G Cuts at: 826 1045 2066 2147 2615 2850 2913 4477 4713 Size: 219 1021 81 468 235 63 1564 236 Cuts at: 4713 4919 5159 6096 6178 7313 7994 8185 826 Size: 206 240 937 82 1135 681 191 1471 Fragments arranged by size: 1564 1471 1135 1021 937 681 468 240 236 235 219 206 191 82 81 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8830 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8830 SmaI CCC'GGG Cuts at: 1872 3870 5108 6157 1872 Size: 1998 1238 1049 4545 Fragments arranged by size: 4545 1998 1238 1049 SmlI C'TyrA_G Cuts at: 820 1017 1943 3376 4807 5948 6934 7835 8097 Size: 197 926 1433 1431 1141 986 901 262 Cuts at: 8097 820 Size: 1553 Fragments arranged by size: 1553 1433 1431 1141 986 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6621 493 Size: 6128 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8830 SphI G_CATG'C Cuts at: 1132 3500 3671 6188 1132 Size: 2368 171 2517 3774 Fragments arranged by size: 3774 2517 2368 171 SspI AAT'ATT Cuts at: 5 52 6754 5 Size: 47 6702 2081 Fragments arranged by size: 6702 2081 47 StuI AGG'CCT Cuts at: 1517 3768 1517 Size: 2251 6579 StyI C'CwwG_G Cuts at: 513 1065 2004 2623 3084 4612 5277 6269 513 Size: 552 939 619 461 1528 665 992 3074 Fragments arranged by size: 3074 1528 992 939 665 619 552 461 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2115 2477 4181 5054 5290 6857 6874 Size: 1255 535 362 1704 873 236 1567 17 Cuts at: 6874 7027 7212 7657 325 Size: 153 185 445 1498 Fragments arranged by size: 1704 1567 1498 1255 873 535 445 362 236 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5472 5604 5733 Size: 274 80 33 51 1443 3495 132 129 Cuts at: 5733 5903 7076 8788 96 Size: 170 1173 1712 138 Fragments arranged by size: 3495 1712 1443 1173 274 170 138 132 129 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3073 3105 3273 3305 3527 3559 3598 3630 5254 Size: 32 168 32 222 32 39 32 1624 Cuts at: 5254 5265 5286 5297 5602 5634 5998 6030 8790 Size: 11 21 11 305 32 364 32 2760 Cuts at: 8790 8822 3073 Size: 32 3081 Fragments arranged by size: 3081 2760 1624 364 305 222 168 39 32 32 32 32 32 32 32 21 11 11 Tth111I GACn'n_nGTC Cuts at: 1316 2837 4187 5618 1316 Size: 1521 1350 1431 4528 Fragments arranged by size: 4528 1521 1431 1350 XbaI T'CTAG_A Cuts at: 6166 6166 Size: 8830 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4994 4994 Size: 8830 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8830 XmaI C'CCGG_G Cuts at: 1870 3868 5106 6155 1870 Size: 1998 1238 1049 4545 Fragments arranged by size: 4545 1998 1238 1049 XmnI GAAnn'nnTTC Cuts at: 2546 2985 3336 3815 6959 2546 Size: 439 351 479 3144 4417 Fragments arranged by size: 4417 3144 479 439 351 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6638 276 Size: 53 83 186 936 5104 2468 Fragments arranged by size: 5104 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbeI BfrBI BsiWI Bsu36I EcoNI EcoRV FseI KasI NarI NruI NsiI PacI PspXI RsrII SanDI SfiI SfoI SrfI SwaI