(Circular) (Six-base) MAPSORT of: pFN21AB7021.seq Check: 14 from: 1 to: 6349 pFN21AB7021 6349 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2352 2352 Size: 6349 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4159 278 Size: 53 83 186 936 2623 2468 Fragments arranged by size: 2623 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3692 1937 Size: 1755 4594 Acc65I G'GTAC_C Cuts at: 3362 3670 3362 Size: 308 6041 AclI AA'CG_TT Cuts at: 1563 4476 4849 1563 Size: 2913 373 3063 Fragments arranged by size: 3063 2913 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2804 4412 5796 6064 1632 Size: 1172 1608 1384 268 1917 Fragments arranged by size: 1917 1608 1384 1172 268 AfeI AGC'GCT Cuts at: 2092 2092 Size: 6349 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6152 AflIII A'CryG_T Cuts at: 1203 5248 6225 1203 Size: 4045 977 1327 Fragments arranged by size: 4045 1327 977 AgeI A'CCGG_T Cuts at: 5180 5180 Size: 6349 AhdI GACnn_n'nnGTC Cuts at: 5078 5078 Size: 6349 AleI CACnn'nnGTG Cuts at: 1166 2576 3291 1166 Size: 1410 715 4224 Fragments arranged by size: 4224 1410 715 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2866 1184 Size: 1682 4667 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2237 2269 2237 Size: 32 6317 AlwNI CAG_nnn'CTG Cuts at: 2455 2635 2689 2772 3112 3388 3526 3613 4092 Size: 180 54 83 340 276 138 87 479 Cuts at: 4092 5664 2455 Size: 1572 3140 Fragments arranged by size: 3140 1572 479 340 276 180 138 87 83 54 ApaI G_GGCC'C Cuts at: 2033 2033 Size: 6349 ApaLI G'TGCA_C Cuts at: 2846 4406 5562 2846 Size: 1560 1156 3633 Fragments arranged by size: 3633 1560 1156 ApoI r'AATT_y Cuts at: 1493 3658 3884 4036 6145 1493 Size: 2165 226 152 2109 1697 Fragments arranged by size: 2165 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4903 160 Size: 872 3871 1606 Fragments arranged by size: 3871 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6349 AvaI C'yCGr_G Cuts at: 1870 1943 2032 3396 3543 3674 1870 Size: 73 89 1364 147 131 4545 Fragments arranged by size: 4545 1364 147 131 89 73 BamHI G'GATC_C Cuts at: 2929 3679 4046 2929 Size: 750 367 5232 Fragments arranged by size: 5232 750 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2176 2581 3059 3081 3123 Size: 325 217 627 389 405 478 22 42 Cuts at: 3123 3196 3262 3362 3670 5125 618 Size: 73 66 100 308 1455 1842 Fragments arranged by size: 1842 1455 627 478 405 389 325 308 217 100 73 66 42 22 BanII G_rGCy'C Cuts at: 1391 2033 2718 3404 3668 1391 Size: 642 685 686 264 4072 Fragments arranged by size: 4072 686 685 642 264 BbeI G_GCGC'C Cuts at: 3063 3063 Size: 6349 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5378 BbvCI CC'TCA_GC Cuts at: 3320 3320 Size: 6349 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3524 3558 4540 4574 1903 Size: 34 1587 34 982 34 3678 Fragments arranged by size: 3678 1587 982 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4241 5451 4241 Size: 1210 5139 BclI T'GATC_A Cuts at: 1548 1548 Size: 6349 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4960 136 Size: 107 122 71 631 605 3288 1525 Fragments arranged by size: 3288 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3477 6344 3477 Size: 2867 3482 BlpI GC'TnA_GC Cuts at: 2712 3766 2712 Size: 1054 5295 Bme1580I G_kGCm'C Cuts at: 1790 2033 2181 2383 2850 4410 5566 1790 Size: 243 148 202 467 1560 1156 2573 Fragments arranged by size: 2573 1560 1156 467 243 202 148 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6349 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2935 3461 4080 Size: 416 53 83 399 24 1518 526 619 Cuts at: 4080 5038 442 Size: 958 1753 Fragments arranged by size: 1753 1518 958 619 526 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6349 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2221 2221 Size: 6349 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2713 3161 5009 1239 Size: 1474 448 1848 2579 Fragments arranged by size: 2579 1848 1474 448 Bpu10I CC'TnA_GC Cuts at: 3320 3320 Size: 6349 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2393 4474 5339 5637 2393 Size: 2081 865 298 3105 Fragments arranged by size: 3105 2081 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5012 882 Size: 617 3513 2219 Fragments arranged by size: 3513 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2884 4140 493 Size: 711 1680 1256 2702 Fragments arranged by size: 2702 1680 1256 711 BsaBI GATnn'nnATC Cuts at: 6343 6343 Size: 6349 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2111 3060 3573 4156 Size: 53 83 186 936 578 949 513 583 Cuts at: 4156 4538 5173 275 Size: 382 635 1451 Fragments arranged by size: 1451 949 936 635 583 578 513 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4782 5180 5454 5601 6012 4782 Size: 398 274 147 411 5119 Fragments arranged by size: 5119 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6124 Fragments arranged by size: 6124 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3531 1186 Size: 2345 4004 BseYI C'CCAG_C Cuts at: 2126 2698 2754 2830 2895 3354 3566 3576 3593 Size: 572 56 76 65 459 212 10 17 Cuts at: 3593 5552 6048 2126 Size: 1959 496 2427 Fragments arranged by size: 2427 1959 572 496 459 212 76 65 56 17 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2648 2758 3098 3401 3554 1163 Size: 573 912 110 340 303 153 3958 Fragments arranged by size: 3958 912 573 340 303 153 110 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3301 3799 4560 5588 1540 Size: 275 186 1300 498 761 1028 2301 Fragments arranged by size: 2301 1300 1028 761 498 275 186 BsiHKAI G_wGCw'C Cuts at: 2622 2850 4410 4495 5566 2622 Size: 228 1560 85 1071 3405 Fragments arranged by size: 3405 1560 1071 228 85 BsmI GAATG_Cn' Cuts at: 2227 3860 3953 2227 Size: 1633 93 4623 Fragments arranged by size: 4623 1633 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2181 2383 2622 2718 2850 3404 Size: 399 243 148 202 239 96 132 554 Cuts at: 3404 3668 4410 4495 5566 1391 Size: 264 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 554 399 264 243 239 202 148 132 96 85 BspEI T'CCGG_A Cuts at: 6012 6012 Size: 6349 BspHI T'CATG_A Cuts at: 4238 5968 4238 Size: 1730 4619 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2196 2352 2454 2511 2643 3704 844 Size: 1352 156 102 57 132 1061 3489 Fragments arranged by size: 3489 1352 1061 156 132 102 57 BsrBI CCG'CTC Cuts at: 1394 1823 2803 4236 1394 Size: 429 980 1433 3507 Fragments arranged by size: 3507 1433 980 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4844 5018 65 Size: 1183 3596 174 1396 Fragments arranged by size: 3596 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3296 4993 5180 1700 Size: 110 1486 1697 187 2869 Fragments arranged by size: 2869 1697 1486 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6349 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6349 BssSI C'ACGA_G Cuts at: 4409 5421 4409 Size: 1012 5337 BstAPI GCAn_nnn'nTGC Cuts at: 794 2455 2626 2635 2980 3388 794 Size: 1661 171 9 345 408 3755 Fragments arranged by size: 3755 1661 408 345 171 9 BstBI TT'CG_AA Cuts at: 5153 5153 Size: 6349 BstEII G'GTnAC_C Cuts at: 1184 3253 1184 Size: 2069 4280 BstXI CCAn_nnnn'nTGG Cuts at: 2903 2903 Size: 6349 BstYI r'GATC_y Cuts at: 1972 2238 2929 3345 3477 3637 3679 4046 4431 Size: 266 691 416 132 160 42 367 385 Cuts at: 4431 4448 5900 5986 5998 6344 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 691 416 385 367 346 266 160 132 86 42 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2574 3036 3167 513 Size: 552 939 570 462 131 3695 Fragments arranged by size: 3695 939 570 552 462 131 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5610 Fragments arranged by size: 5610 683 56 BtsI GCAGTG_nn' Cuts at: 2453 2709 2848 2850 3110 3463 3865 4659 4679 Size: 256 139 2 260 353 402 794 20 Cuts at: 4679 2453 Size: 4123 Fragments arranged by size: 4123 794 402 353 260 256 139 20 2 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6349 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2653 2688 529 Size: 35 2089 35 4190 Fragments arranged by size: 4190 2089 35 35 DraI TTT'AAA Cuts at: 3653 3653 Size: 6349 DraIII CAC_nnn'GTG Cuts at: 2626 3631 2626 Size: 1005 5344 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5356 809 Size: 507 4040 1802 Fragments arranged by size: 4040 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2433 3298 3433 Size: 54 1425 50 275 18 603 865 135 Cuts at: 3433 3796 4685 6314 8 Size: 363 889 1629 43 Fragments arranged by size: 1629 1425 889 865 603 363 275 135 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3298 3796 1537 Size: 275 1486 498 4090 Fragments arranged by size: 4090 1486 498 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2360 4279 1353 Size: 1007 1919 3423 Fragments arranged by size: 3423 1919 1007 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4936 5310 5456 387 Size: 1064 138 3347 374 146 1280 Fragments arranged by size: 3347 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2713 2804 3161 4412 5009 5796 6064 Size: 393 1081 91 357 1251 597 787 268 Cuts at: 6064 1239 Size: 1524 Fragments arranged by size: 1524 1251 1081 787 597 393 357 268 91 EcoO109I rG'GnC_Cy Cuts at: 2029 2100 2632 3385 2029 Size: 71 532 753 4993 Fragments arranged by size: 4993 753 532 71 EcoRI G'AATT_C Cuts at: 3658 4036 3658 Size: 378 5971 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2413 2413 Size: 6349 FspI TGC'GCA Cuts at: 2984 4855 2984 Size: 1871 4478 HaeII r_GCGC'y Cuts at: 1678 2054 2094 3063 5496 1678 Size: 376 40 969 2433 2531 Fragments arranged by size: 2531 2433 969 376 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4990 5022 5064 5096 1373 Size: 32 135 32 3418 32 42 32 2626 Fragments arranged by size: 3418 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3693 3939 669 Size: 988 281 1755 246 3079 Fragments arranged by size: 3079 1755 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6349 HpaI GTT'AAC Cuts at: 3939 3939 Size: 6349 KasI G'GCGC_C Cuts at: 3059 3059 Size: 6349 KpnI G_GTAC'C Cuts at: 3366 3674 3366 Size: 308 6041 MfeI C'AATT_G Cuts at: 3948 3948 Size: 6349 MluI A'CGCG_T Cuts at: 6225 6225 Size: 6349 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2319 5463 5647 1305 Size: 1014 3144 184 2007 Fragments arranged by size: 3144 2007 1014 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2435 10 Size: 54 1425 343 603 3924 Fragments arranged by size: 3924 1425 603 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2576 3087 3291 3632 4307 4666 4825 Size: 648 1410 511 204 341 675 359 159 Cuts at: 4825 518 Size: 2042 Fragments arranged by size: 2042 1410 675 648 511 359 341 204 159 MspA1I CmG'CkG Cuts at: 1606 2130 2269 2641 2878 2899 3078 3765 4442 Size: 524 139 372 237 21 179 687 677 Cuts at: 4442 5590 5835 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 687 677 524 372 245 237 179 139 21 NaeI GCC'GGC Cuts at: 3298 3298 Size: 6349 NarI GG'CG_CC Cuts at: 3060 3060 Size: 6349 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4858 Fragments arranged by size: 4858 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6349 NgoMIV G'CCGG_C Cuts at: 3296 3296 Size: 6349 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6349 NotI GC'GGCC_GC Cuts at: 3796 3796 Size: 6349 NspI r_CATG'y Cuts at: 1132 1229 3562 3707 5252 1132 Size: 97 2333 145 1545 2229 Fragments arranged by size: 2333 2229 1545 145 97 PasI CC'CwG_GG Cuts at: 1348 3244 1348 Size: 1896 4453 PciI A'CATG_T Cuts at: 5248 5248 Size: 6349 PflMI CCAn_nnn'nTGG Cuts at: 1108 2689 3526 1108 Size: 1581 837 3931 Fragments arranged by size: 3931 1581 837 PfoI T'CCnGG_A Cuts at: 2241 2613 3519 2241 Size: 372 906 5071 Fragments arranged by size: 5071 906 372 PmeI GTTT'AAAC Cuts at: 3653 3653 Size: 6349 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2177 2209 4418 4450 5931 5963 2177 Size: 32 2209 32 1481 32 2563 Fragments arranged by size: 2563 2209 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2632 2632 Size: 6349 PshAI GACnn'nnGTC Cuts at: 1613 2410 1613 Size: 797 5552 PsiI TTA'TAA Cuts at: 3919 3919 Size: 6349 PspOMI G'GGCC_C Cuts at: 2029 2029 Size: 6349 PstI C_TGCA'G Cuts at: 830 2277 2349 2451 2845 3417 3701 830 Size: 1447 72 102 394 572 284 3478 Fragments arranged by size: 3478 1447 572 394 284 102 72 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6349 PvuII CAG'CTG Cuts at: 2130 2878 2899 2130 Size: 748 21 5580 Fragments arranged by size: 5580 748 21 SalI G'TCGA_C Cuts at: 1936 3691 1936 Size: 1755 4594 SbfI CC_TGCA'GG Cuts at: 3701 3701 Size: 6349 ScaI AGT'ACT Cuts at: 4597 6309 4597 Size: 1712 4637 SfcI C'TryA_G Cuts at: 826 1045 2273 2345 2447 2841 3413 3697 4832 Size: 219 1228 72 102 394 572 284 1135 Cuts at: 4832 5513 5704 826 Size: 681 191 1471 Fragments arranged by size: 1471 1228 1135 681 572 394 284 219 191 102 72 SfoI GGC'GCC Cuts at: 3061 3061 Size: 6349 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6349 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6349 SmaI CCC'GGG Cuts at: 1872 2034 3398 3676 1872 Size: 162 1364 278 4545 Fragments arranged by size: 4545 1364 278 162 SmlI C'TyrA_G Cuts at: 820 1017 1943 2408 4453 5354 5616 820 Size: 197 926 465 2045 901 262 1553 Fragments arranged by size: 2045 1553 926 901 465 262 197 SnaBI TAC'GTA Cuts at: 493 4140 493 Size: 3647 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6349 SphI G_CATG'C Cuts at: 1132 3707 1132 Size: 2575 3774 SspI AAT'ATT Cuts at: 5 52 4273 5 Size: 47 4221 2081 Fragments arranged by size: 4221 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6349 StyI C'CwwG_G Cuts at: 513 1065 2004 3051 3788 513 Size: 552 939 1047 737 3074 Fragments arranged by size: 3074 1047 939 737 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2599 3239 3419 3488 4376 4393 4546 Size: 1255 1019 640 180 69 888 17 153 Cuts at: 4546 4731 5176 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1019 888 640 445 185 180 153 69 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4595 6307 96 Size: 274 80 33 51 1443 2618 1712 138 Fragments arranged by size: 2618 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6309 6341 6309 Size: 32 6317 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6349 XbaI T'CTAG_A Cuts at: 3685 3685 Size: 6349 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2575 2575 Size: 6349 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6349 XmaI C'CCGG_G Cuts at: 1870 2032 3396 3674 1870 Size: 162 1364 278 4545 Fragments arranged by size: 4545 1364 278 162 XmnI GAAnn'nnTTC Cuts at: 3092 4478 3092 Size: 1386 4963 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4157 276 Size: 53 83 186 936 2623 2468 Fragments arranged by size: 2623 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI