(Circular) (Six-base) MAPSORT of: pFN21AB7020.seq Check: 7461 from: 1 to: 7699 pFN21AB7020 7699 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2732 2969 2732 Size: 237 7462 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5509 278 Size: 53 83 186 936 3973 2468 Fragments arranged by size: 3973 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2319 3955 5042 1937 Size: 382 1636 1087 4594 Fragments arranged by size: 4594 1636 1087 382 Acc65I G'GTAC_C Cuts at: 5020 5020 Size: 7699 AclI AA'CG_TT Cuts at: 1563 5826 6199 1563 Size: 4263 373 3063 Fragments arranged by size: 4263 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3387 4025 5762 7146 7414 1632 Size: 1755 638 1737 1384 268 1917 Fragments arranged by size: 1917 1755 1737 1384 638 268 AfeI AGC'GCT Cuts at: 4726 4726 Size: 7699 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7502 AflIII A'CryG_T Cuts at: 1203 3891 6598 7575 1203 Size: 2688 2707 977 1327 Fragments arranged by size: 2707 2688 1327 977 AgeI A'CCGG_T Cuts at: 6530 6530 Size: 7699 AhdI GACnn_n'nnGTC Cuts at: 6428 6428 Size: 7699 AleI CACnn'nnGTG Cuts at: 1166 2534 3017 1166 Size: 1368 483 5848 Fragments arranged by size: 5848 1368 483 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3035 3822 1184 Size: 1851 787 5061 Fragments arranged by size: 5061 1851 787 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2318 2350 2318 Size: 32 7667 AlwNI CAG_nnn'CTG Cuts at: 2955 3009 3590 4984 5442 7014 2955 Size: 54 581 1394 458 1572 3640 Fragments arranged by size: 3640 1572 1394 581 458 54 ApaI G_GGCC'C Cuts at: 2904 3655 2904 Size: 751 6948 ApaLI G'TGCA_C Cuts at: 4268 5756 6912 4268 Size: 1488 1156 5055 Fragments arranged by size: 5055 1488 1156 ApoI r'AATT_y Cuts at: 1493 3932 4655 5008 5234 5386 7495 1493 Size: 2439 723 353 226 152 2109 1697 Fragments arranged by size: 2439 2109 1697 723 353 226 152 AseI AT'TA_AT Cuts at: 160 1032 6253 160 Size: 872 5221 1606 Fragments arranged by size: 5221 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7699 AvaI C'yCGr_G Cuts at: 1870 1943 2672 3033 3074 3090 3298 4094 5024 Size: 73 729 361 41 16 208 796 930 Cuts at: 5024 1870 Size: 4545 Fragments arranged by size: 4545 930 796 729 361 208 73 41 16 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2914 2947 2914 Size: 33 7666 BamHI G'GATC_C Cuts at: 5029 5396 5029 Size: 367 7332 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2594 2720 2752 2794 2804 Size: 325 217 627 807 126 32 42 10 Cuts at: 2804 3424 5020 6475 618 Size: 620 1596 1455 1842 Fragments arranged by size: 1842 1596 1455 807 627 620 325 217 126 42 32 10 BanII G_rGCy'C Cuts at: 1391 2017 2331 2697 2904 3090 3311 3655 3692 Size: 626 314 366 207 186 221 344 37 Cuts at: 3692 5018 1391 Size: 1326 4072 Fragments arranged by size: 4072 1326 626 366 344 314 221 207 186 37 BbeI G_GCGC'C Cuts at: 2598 2598 Size: 7699 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3703 4899 928 Size: 971 1804 1196 3728 Fragments arranged by size: 3728 1804 1196 971 BbvCI CC'TCA_GC Cuts at: 2431 3163 2431 Size: 732 6967 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4265 4299 5890 5924 1903 Size: 34 2328 34 1591 34 3678 Fragments arranged by size: 3678 2328 1591 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4961 5591 6801 4961 Size: 630 1210 5859 Fragments arranged by size: 5859 1210 630 BclI T'GATC_A Cuts at: 1548 4072 1548 Size: 2524 5175 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2047 4427 6310 Size: 107 122 71 631 605 375 2380 1883 Cuts at: 6310 136 Size: 1525 Fragments arranged by size: 2380 1883 1525 631 605 375 122 107 71 BglII A'GATC_T Cuts at: 7694 7694 Size: 7699 BlpI GC'TnA_GC Cuts at: 5116 5116 Size: 7699 Bme1580I G_kGCm'C Cuts at: 1790 2904 3375 3427 3580 3655 4272 5760 6916 Size: 1114 471 52 153 75 617 1488 1156 Cuts at: 6916 1790 Size: 2573 Fragments arranged by size: 2573 1488 1156 1114 617 471 153 75 52 BmgBI CAC'GTC Cuts at: 1318 3892 1318 Size: 2574 5125 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2694 2845 4531 Size: 416 53 83 399 24 1277 151 1686 Cuts at: 4531 5430 6388 442 Size: 899 958 1753 Fragments arranged by size: 1753 1686 1277 958 899 416 399 151 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7699 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2518 2716 3661 2518 Size: 198 945 6556 Fragments arranged by size: 6556 945 198 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3174 3647 4373 4489 6359 1239 Size: 1935 473 726 116 1870 2579 Fragments arranged by size: 2579 1935 1870 726 473 116 Bpu10I CC'TnA_GC Cuts at: 2431 3163 3397 4043 4943 2431 Size: 732 234 646 900 5187 Fragments arranged by size: 5187 900 732 646 234 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2315 2744 4694 5824 6689 6987 2315 Size: 429 1950 1130 865 298 3027 Fragments arranged by size: 3027 1950 1130 865 429 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2117 2381 2669 3620 6362 882 Size: 617 618 264 288 951 2742 2219 Fragments arranged by size: 2742 2219 951 618 617 288 264 BsaAI yAC'GTr Cuts at: 493 1204 2323 2467 5490 493 Size: 711 1119 144 3023 2702 Fragments arranged by size: 3023 2702 1119 711 144 BsaBI GATnn'nnATC Cuts at: 4406 7693 4406 Size: 3287 4412 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2595 3552 5506 5888 Size: 53 83 186 936 1062 957 1954 382 Cuts at: 5888 6523 275 Size: 635 1451 Fragments arranged by size: 1954 1451 1062 957 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4638 6132 6530 6804 6951 7362 4638 Size: 1494 398 274 147 411 4975 Fragments arranged by size: 4975 1494 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2318 2348 2837 2867 4734 Size: 30 165 30 915 30 489 30 1867 Cuts at: 4734 4764 1178 Size: 30 4113 Fragments arranged by size: 4113 1867 915 489 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2428 3926 1186 Size: 1242 1498 4959 Fragments arranged by size: 4959 1498 1242 BseYI C'CCAG_C Cuts at: 2941 3211 3654 3701 3926 4626 6902 7398 2941 Size: 270 443 47 225 700 2276 496 3242 Fragments arranged by size: 3242 2276 700 496 443 270 225 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2330 2794 2941 2995 3003 3299 3371 Size: 573 594 464 147 54 8 296 72 Cuts at: 3371 3548 4589 5012 1163 Size: 177 1041 423 3850 Fragments arranged by size: 3850 1041 594 573 464 423 296 177 147 72 54 8 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2521 3333 5149 5910 6938 1540 Size: 275 186 520 812 1816 761 1028 2301 Fragments arranged by size: 2301 1816 1028 812 761 520 275 186 BsiHKAI G_wGCw'C Cuts at: 2331 4272 5760 5845 6916 2331 Size: 1941 1488 85 1071 3114 Fragments arranged by size: 3114 1941 1488 1071 85 BsmI GAATG_Cn' Cuts at: 5210 5303 5210 Size: 93 7606 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2017 2331 2697 2904 3090 3311 3375 Size: 399 227 314 366 207 186 221 64 Cuts at: 3375 3427 3580 3655 3692 4272 5018 5760 5845 Size: 52 153 75 37 580 746 742 85 Cuts at: 5845 6916 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 746 742 580 399 366 314 227 221 207 186 153 85 75 64 52 37 BspEI T'CCGG_A Cuts at: 7362 7362 Size: 7699 BspHI T'CATG_A Cuts at: 4497 5588 7318 4497 Size: 1091 1730 4878 Fragments arranged by size: 4878 1730 1091 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2732 2969 4905 4984 5054 844 Size: 1888 237 1936 79 70 3489 Fragments arranged by size: 3489 1936 1888 237 79 70 BsrBI CCG'CTC Cuts at: 1394 1823 3436 5586 1394 Size: 429 1613 2150 3507 Fragments arranged by size: 3507 2150 1613 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2481 2709 3804 4227 4702 4716 6194 Size: 1183 1233 228 1095 423 475 14 1478 Cuts at: 6194 6368 65 Size: 174 1396 Fragments arranged by size: 1478 1396 1233 1183 1095 475 423 228 174 14 BsrFI r'CCGG_y Cuts at: 1700 1810 2102 2516 2822 4235 4569 4922 6343 Size: 110 292 414 306 1413 334 353 1421 Cuts at: 6343 6530 1700 Size: 187 2869 Fragments arranged by size: 2869 1421 1413 414 353 334 306 292 187 110 BsrGI T'GTAC_A Cuts at: 96 3120 96 Size: 3024 4675 BssHII G'CGCG_C Cuts at: 1925 2620 1925 Size: 695 7004 BssSI C'ACGA_G Cuts at: 5759 6771 5759 Size: 1012 6687 BstAPI GCAn_nnn'nTGC Cuts at: 794 3141 794 Size: 2347 5352 BstBI TT'CG_AA Cuts at: 6503 6503 Size: 7699 BstEII G'GTnAC_C Cuts at: 1184 4105 4912 1184 Size: 2921 807 3971 Fragments arranged by size: 3971 2921 807 BstXI CCAn_nnnn'nTGG Cuts at: 2704 3152 4390 4819 2704 Size: 448 1238 429 5584 Fragments arranged by size: 5584 1238 448 429 BstYI r'GATC_y Cuts at: 1972 4401 4938 5029 5396 5781 5798 7250 7336 Size: 2429 537 91 367 385 17 1452 86 Cuts at: 7336 7348 7694 1972 Size: 12 346 1977 Fragments arranged by size: 2429 1977 1452 537 385 367 346 91 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3749 4646 3749 Size: 897 6802 BtgI C'CryG_G Cuts at: 513 1065 2004 2739 3145 3949 4554 4584 513 Size: 552 939 735 406 804 605 30 3628 Fragments arranged by size: 3628 939 804 735 605 552 406 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2436 2793 3478 3718 487 Size: 683 56 1210 357 685 240 4468 Fragments arranged by size: 4468 1210 685 683 357 240 56 BtsI GCAGTG_nn' Cuts at: 4601 5215 6009 6029 4601 Size: 614 794 20 6271 Fragments arranged by size: 6271 794 614 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7699 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7664 DraI TTT'AAA Cuts at: 5003 5003 Size: 7699 DraIII CAC_nnn'GTG Cuts at: 3179 3179 Size: 7699 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4763 6706 809 Size: 507 3447 1943 1802 Fragments arranged by size: 3447 1943 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2518 2824 Size: 54 1425 50 275 18 177 511 306 Cuts at: 2824 3103 3330 3970 3990 4118 4920 4924 5146 Size: 279 227 640 20 128 802 4 222 Cuts at: 5146 6035 7664 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 802 640 511 306 279 275 227 222 177 128 54 50 43 20 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2518 3330 5146 1537 Size: 275 706 812 1816 4090 Fragments arranged by size: 4090 1816 812 706 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2115 2187 2405 2672 3476 5629 1353 Size: 762 72 218 267 804 2153 3423 Fragments arranged by size: 3423 2153 804 762 267 218 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2705 6286 6660 6806 387 Size: 1064 138 1116 3581 374 146 1280 Fragments arranged by size: 3581 1280 1116 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2329 2329 Size: 7699 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3174 3387 3647 4025 4373 4489 5762 Size: 393 1542 213 260 378 348 116 1273 Cuts at: 5762 6359 7146 7414 1239 Size: 597 787 268 1524 Fragments arranged by size: 1542 1524 1273 787 597 393 378 348 268 260 213 116 EcoNI CCTnn'n_nnAGG Cuts at: 4046 4046 Size: 7699 EcoO109I rG'GnC_Cy Cuts at: 2900 2901 3094 3159 3651 3850 2900 Size: 1 193 65 492 199 6749 Fragments arranged by size: 6749 492 199 193 65 1 EcoRI G'AATT_C Cuts at: 5008 5386 5008 Size: 378 7321 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2407 2584 4855 2407 Size: 177 2271 5251 Fragments arranged by size: 5251 2271 177 FseI GG_CCGG'CC Cuts at: 4926 4926 Size: 7699 FspI TGC'GCA Cuts at: 2540 6205 2540 Size: 3665 4034 HaeII r_GCGC'y Cuts at: 1678 2117 2598 3229 4728 6846 1678 Size: 439 481 631 1499 2118 2531 Fragments arranged by size: 2531 2118 1499 631 481 439 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2158 2190 2414 2446 2978 Size: 32 135 32 586 32 224 32 532 Cuts at: 2978 3010 3027 3059 4349 4381 6340 6372 6414 Size: 32 17 32 1290 32 1959 32 42 Cuts at: 6414 6446 1373 Size: 32 2626 Fragments arranged by size: 2626 1959 1290 586 532 224 135 42 32 32 32 32 32 32 32 32 32 17 HincII GTy'rAC Cuts at: 669 1657 1938 2527 3289 3956 4303 5043 5289 Size: 988 281 589 762 667 347 740 246 Cuts at: 5289 669 Size: 3079 Fragments arranged by size: 3079 988 762 740 667 589 347 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7699 HpaI GTT'AAC Cuts at: 5289 5289 Size: 7699 KasI G'GCGC_C Cuts at: 2594 2594 Size: 7699 KpnI G_GTAC'C Cuts at: 5024 5024 Size: 7699 MfeI C'AATT_G Cuts at: 5298 5298 Size: 7699 MluI A'CGCG_T Cuts at: 7575 7575 Size: 7699 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6813 6997 1305 Size: 5508 184 2007 Fragments arranged by size: 5508 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 3992 10 Size: 54 1425 343 177 1983 3717 Fragments arranged by size: 3717 1983 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2534 2615 3017 4817 5657 6016 6175 Size: 648 1368 81 402 1800 840 359 159 Cuts at: 6175 518 Size: 2042 Fragments arranged by size: 2042 1800 1368 840 648 402 359 159 81 MspA1I CmG'CkG Cuts at: 1606 2059 2071 2086 2314 2837 3211 3532 3701 Size: 453 12 15 228 523 374 321 169 Cuts at: 3701 4030 4207 4413 5115 5792 6940 7185 1606 Size: 329 177 206 702 677 1148 245 2120 Fragments arranged by size: 2120 1148 702 677 523 453 374 329 321 245 228 206 177 169 15 12 NaeI GCC'GGC Cuts at: 2104 2518 4237 4924 2104 Size: 414 1719 687 4879 Fragments arranged by size: 4879 1719 687 414 NarI GG'CG_CC Cuts at: 2595 2595 Size: 7699 NcoI C'CATG_G Cuts at: 513 1065 2004 3145 4584 513 Size: 552 939 1141 1439 3628 Fragments arranged by size: 3628 1439 1141 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7699 NgoMIV G'CCGG_C Cuts at: 2102 2516 4235 4922 2102 Size: 414 1719 687 4879 Fragments arranged by size: 4879 1719 687 414 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7699 NotI GC'GGCC_GC Cuts at: 5146 5146 Size: 7699 NspI r_CATG'y Cuts at: 1132 1229 2605 4219 4741 4963 5057 6602 1132 Size: 97 1376 1614 522 222 94 1545 2229 Fragments arranged by size: 2229 1614 1545 1376 522 222 97 94 PasI CC'CwG_GG Cuts at: 1348 2870 4390 1348 Size: 1522 1520 4657 Fragments arranged by size: 4657 1522 1520 PciI A'CATG_T Cuts at: 6598 6598 Size: 7699 PflMI CCAn_nnn'nTGG Cuts at: 1108 2183 3250 4210 4413 1108 Size: 1075 1067 960 203 4394 Fragments arranged by size: 4394 1075 1067 960 203 PfoI T'CCnGG_A Cuts at: 2583 3453 3663 3811 4350 4752 4953 2583 Size: 870 210 148 539 402 201 5329 Fragments arranged by size: 5329 870 539 402 210 201 148 PmeI GTTT'AAAC Cuts at: 5003 5003 Size: 7699 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2318 2350 5768 5800 7281 7313 2318 Size: 32 3418 32 1481 32 2704 Fragments arranged by size: 3418 2704 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3094 3850 3094 Size: 756 6943 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7699 PsiI TTA'TAA Cuts at: 5269 5269 Size: 7699 PspOMI G'GGCC_C Cuts at: 2900 3651 2900 Size: 751 6948 PspXI vC'TCGA_Gb Cuts at: 3074 4094 3074 Size: 1020 6679 PstI C_TGCA'G Cuts at: 830 3798 4971 5051 830 Size: 2968 1173 80 3478 Fragments arranged by size: 3478 2968 1173 80 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7699 PvuII CAG'CTG Cuts at: 2837 3211 3532 3701 4207 2837 Size: 374 321 169 506 6329 Fragments arranged by size: 6329 506 374 321 169 SacI G_AGCT'C Cuts at: 2331 2331 Size: 7699 SalI G'TCGA_C Cuts at: 1936 3954 5041 1936 Size: 2018 1087 4594 Fragments arranged by size: 4594 2018 1087 SapI GCTCTTCn'nnn_ Cuts at: 3476 3476 Size: 7699 SbfI CC_TGCA'GG Cuts at: 5051 5051 Size: 7699 ScaI AGT'ACT Cuts at: 3473 5947 7659 3473 Size: 2474 1712 3513 Fragments arranged by size: 3513 2474 1712 SexAI A'CCwGG_T Cuts at: 2238 4311 4869 2238 Size: 2073 558 5068 Fragments arranged by size: 5068 2073 558 SfcI C'TryA_G Cuts at: 826 1045 3696 3794 4967 5047 6182 6863 7054 Size: 219 2651 98 1173 80 1135 681 191 Cuts at: 7054 826 Size: 1471 Fragments arranged by size: 2651 1471 1173 1135 681 219 191 98 80 SfoI GGC'GCC Cuts at: 2596 2596 Size: 7699 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7699 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7699 SmaI CCC'GGG Cuts at: 1872 3092 5026 1872 Size: 1220 1934 4545 Fragments arranged by size: 4545 1934 1220 SmlI C'TyrA_G Cuts at: 820 1017 1943 2330 2759 3074 4094 4709 5803 Size: 197 926 387 429 315 1020 615 1094 Cuts at: 5803 6704 6966 820 Size: 901 262 1553 Fragments arranged by size: 1553 1094 1020 926 901 615 429 387 315 262 197 SnaBI TAC'GTA Cuts at: 493 5490 493 Size: 4997 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7699 SphI G_CATG'C Cuts at: 1132 5057 1132 Size: 3925 3774 SspI AAT'ATT Cuts at: 5 52 5623 5 Size: 47 5571 2081 Fragments arranged by size: 5571 2081 47 StuI AGG'CCT Cuts at: 1517 4052 1517 Size: 2535 5164 StyI C'CwwG_G Cuts at: 513 1065 2004 2532 2559 2841 3145 3879 4584 Size: 552 939 528 27 282 304 734 705 Cuts at: 4584 5138 513 Size: 554 3074 Fragments arranged by size: 3074 939 734 705 554 552 528 304 282 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2175 2792 3089 4597 4618 5726 5743 Size: 1255 595 617 297 1508 21 1108 17 Cuts at: 5743 5896 6081 6526 325 Size: 153 185 445 1498 Fragments arranged by size: 1508 1498 1255 1108 617 595 445 297 185 153 21 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3120 3471 3603 Size: 274 80 33 51 1443 1143 351 132 Cuts at: 3603 3780 3801 5945 7657 96 Size: 177 21 2144 1712 138 Fragments arranged by size: 2144 1712 1443 1143 351 274 177 138 132 80 51 33 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2837 2869 4734 4766 7659 7691 2837 Size: 32 1865 32 2893 32 2845 Fragments arranged by size: 2893 2845 1865 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7699 XbaI T'CTAG_A Cuts at: 5035 5035 Size: 7699 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2184 2184 Size: 7699 XhoI C'TCGA_G Cuts at: 1943 3074 4094 1943 Size: 1131 1020 5548 Fragments arranged by size: 5548 1131 1020 XmaI C'CCGG_G Cuts at: 1870 3090 5024 1870 Size: 1220 1934 4545 Fragments arranged by size: 4545 1934 1220 XmnI GAAnn'nnTTC Cuts at: 5828 5828 Size: 7699 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5507 276 Size: 53 83 186 936 3973 2468 Fragments arranged by size: 3973 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BfrBI BsiWI BsmBI BstZ17I EcoRV FspAI NruI NsiI PacI PmlI PsrI RsrII SacII SanDI SfiI SrfI SwaI