Restriction map for
pFN21AB7019
(Circular) (Six-base) MAPSORT of: pFN21AB7019.seq Check: 769 from: 1 to: 6958
pFN21AB7019 6958 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4768 278
Size: 53 83 186 936 3232 2468
Fragments arranged by size:
3232 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3290 4301 1937
Size: 1353 1011 4594
Fragments arranged by size:
4594 1353 1011
Acc65I G'GTAC_C
Cuts at: 4279 4279
Size: 6958
AclI AA'CG_TT
Cuts at: 1563 5085 5458 1563
Size: 3522 373 3063
Fragments arranged by size:
3522 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2122 5021 6405 6673 1632
Size: 490 2899 1384 268 1917
Fragments arranged by size:
2899 1917 1384 490 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6761
AflIII A'CryG_T
Cuts at: 1203 2683 5857 6834 1203
Size: 1480 3174 977 1327
Fragments arranged by size:
3174 1480 1327 977
AgeI A'CCGG_T
Cuts at: 5789 5789
Size: 6958
AhdI GACnn_n'nnGTC
Cuts at: 4024 5687 4024
Size: 1663 5295
AleI CACnn'nnGTG
Cuts at: 1166 2337 1166
Size: 1171 5787
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6958
AlwNI CAG_nnn'CTG
Cuts at: 2591 4701 6273 2591
Size: 2110 1572 3276
Fragments arranged by size:
3276 2110 1572
ApaI G_GGCC'C
Cuts at: 3081 3081
Size: 6958
ApaLI G'TGCA_C
Cuts at: 5015 6171 5015
Size: 1156 5802
ApoI r'AATT_y
Cuts at: 1493 3111 3474 4267 4493 4645 6754 1493
Size: 1618 363 793 226 152 2109 1697
Fragments arranged by size:
2109 1697 1618 793 363 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2177 5512 160
Size: 872 1145 3335 1606
Fragments arranged by size:
3335 1606 1145 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6958
AvaI C'yCGr_G
Cuts at: 1870 1943 2950 4283 1870
Size: 73 1007 1333 4545
Fragments arranged by size:
4545 1333 1007 73
BamHI G'GATC_C
Cuts at: 4288 4655 4288
Size: 367 6591
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4279 5734 618
Size: 325 217 627 2492 1455 1842
Fragments arranged by size:
2492 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3081 3420 4277 1391
Size: 1690 339 857 4072
Fragments arranged by size:
4072 1690 857 339
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2895 3664 928
Size: 971 996 769 4222
Fragments arranged by size:
4222 996 971 769
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5149 5183 1903
Size: 34 3212 34 3678
Fragments arranged by size:
3678 3212 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4850 6060 4850
Size: 1210 5748
BclI T'GATC_A
Cuts at: 1548 2538 2635 1548
Size: 990 97 5871
Fragments arranged by size:
5871 990 97
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5569 136
Size: 107 122 71 631 605 3897 1525
Fragments arranged by size:
3897 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6953 6953
Size: 6958
BlpI GC'TnA_GC
Cuts at: 4375 4375
Size: 6958
Bme1580I G_kGCm'C
Cuts at: 1790 3081 3784 5019 6175 1790
Size: 1291 703 1235 1156 2573
Fragments arranged by size:
2573 1291 1235 1156 703
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6958
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3648 4689 5647
Size: 416 53 83 399 24 2231 1041 958
Cuts at: 5647 442
Size: 1753
Fragments arranged by size:
2231 1753 1041 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6958
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2572 2572
Size: 6958
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3446 3794 5618 1239
Size: 2207 348 1824 2579
Fragments arranged by size:
2579 2207 1824 348
Bpu10I CC'TnA_GC
Cuts at: 2718 2718
Size: 6958
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5083 5948 6246 5083
Size: 865 298 5795
Fragments arranged by size:
5795 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5621 882
Size: 617 4122 2219
Fragments arranged by size:
4122 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4749 493
Size: 711 3545 2702
Fragments arranged by size:
3545 2702 711
BsaBI GATnn'nnATC
Cuts at: 2187 6952 2187
Size: 4765 2193
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4765 5147 5782 275
Size: 53 83 186 936 3232 382 635 1451
Fragments arranged by size:
3232 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2013 2196 5391 5789 6063 6210 6621 2013
Size: 183 3195 398 274 147 411 2350
Fragments arranged by size:
3195 2350 411 398 274 183 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3870 3900 1178
Size: 30 165 30 2467 30 4236
Fragments arranged by size:
4236 2467 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2455 2561 3159 3443 3664 1186
Size: 1269 106 598 284 221 4480
Fragments arranged by size:
4480 1269 598 284 221 106
BseYI C'CCAG_C
Cuts at: 2073 6161 6657 2073
Size: 4088 496 2374
Fragments arranged by size:
4088 2374 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3521 3761 1163
Size: 573 1785 240 4360
Fragments arranged by size:
4360 1785 573 240
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4408 5169 6197 1540
Size: 275 186 2407 761 1028 2301
Fragments arranged by size:
2407 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2768 2876 3420 5019 5104 6175 2768
Size: 108 544 1599 85 1071 3551
Fragments arranged by size:
3551 1599 1071 544 108 85
BsmI GAATG_Cn'
Cuts at: 2009 2309 2626 4091 4469 4562 2009
Size: 300 317 1465 378 93 4405
Fragments arranged by size:
4405 1465 378 317 300 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2768 2876 3081 3420 3784 4277 5019
Size: 399 978 108 205 339 364 493 742
Cuts at: 5019 5104 6175 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 978 742 493 399 364 339
205 108 85
BspEI T'CCGG_A
Cuts at: 2013 6621 2013
Size: 4608 2350
BspHI T'CATG_A
Cuts at: 2463 4847 6577 2463
Size: 2384 1730 2844
Fragments arranged by size:
2844 2384 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4313 844
Size: 3469 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3897 4845 1394
Size: 429 2074 948 3507
Fragments arranged by size:
3507 2074 948 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3933 5453 5627 65
Size: 1183 2685 1520 174 1396
Fragments arranged by size:
2685 1520 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5602 5789 1700
Size: 110 3792 187 2869
Fragments arranged by size:
3792 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3225 96
Size: 3129 3829
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6958
BssSI C'ACGA_G
Cuts at: 5018 6030 5018
Size: 1012 5946
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6958
BstBI TT'CG_AA
Cuts at: 2602 5762 2602
Size: 3160 3798
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6958
BstXI CCAn_nnnn'nTGG
Cuts at: 3554 3772 3554
Size: 218 6740
BstYI r'GATC_y
Cuts at: 1972 2066 2323 4288 4655 5040 5057 6509 6595
Size: 94 257 1965 367 385 17 1452 86
Cuts at: 6595 6607 6953 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1965 1452 385 367 346 257 94
86 17 12
BstZ17I GTA'TAC
Cuts at: 3291 3291
Size: 6958
BtgI C'CryG_G
Cuts at: 513 1065 2004 4031 513
Size: 552 939 2027 3440
Fragments arranged by size:
3440 2027 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6219
Fragments arranged by size:
6219 683 56
BtsI GCAGTG_nn'
Cuts at: 3928 4474 5268 5288 3928
Size: 546 794 20 5598
Fragments arranged by size:
5598 794 546 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6958
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6923
DraI TTT'AAA
Cuts at: 4262 4262
Size: 6958
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5965 809
Size: 507 4649 1802
Fragments arranged by size:
4649 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2276 3610 4405
Size: 54 1425 50 275 18 446 1334 795
Cuts at: 4405 5294 6923 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1334 889 795 446 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4405 1537
Size: 275 2593 4090
Fragments arranged by size:
4090 2593 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4888 1353
Size: 3535 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5545 5919 6065 387
Size: 1064 138 3956 374 146 1280
Fragments arranged by size:
3956 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3418 3418
Size: 6958
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2122 3446 3794 5021 5618 6405 6673
Size: 393 490 1324 348 1227 597 787 268
Cuts at: 6673 1239
Size: 1524
Fragments arranged by size:
1524 1324 1227 787 597 490 393 348
268
EcoO109I rG'GnC_Cy
Cuts at: 3077 3589 4204 3077
Size: 512 615 5831
Fragments arranged by size:
5831 615 512
EcoRI G'AATT_C
Cuts at: 3111 4267 4645 3111
Size: 1156 378 5424
Fragments arranged by size:
5424 1156 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2743 3994 2743
Size: 1251 5707
FspI TGC'GCA
Cuts at: 5464 5464
Size: 6958
HaeII r_GCGC'y
Cuts at: 1678 2406 6105 1678
Size: 728 3699 2531
Fragments arranged by size:
3699 2531 728
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2356 2388 3085 3117 3324
Size: 32 135 32 784 32 697 32 207
Cuts at: 3324 3356 3876 3908 4010 4042 5599 5631 5673
Size: 32 520 32 102 32 1557 32 42
Cuts at: 5673 5705 1373
Size: 32 2626
Fragments arranged by size:
2626 1557 784 697 520 207 135 102
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2836 4302 4548 669
Size: 988 281 898 1466 246 3079
Fragments arranged by size:
3079 1466 988 898 281 246
HindIII A'AGCT_T
Cuts at: 748 2259 748
Size: 1511 5447
HpaI GTT'AAC
Cuts at: 4548 4548
Size: 6958
KpnI G_GTAC'C
Cuts at: 4283 4283
Size: 6958
MfeI C'AATT_G
Cuts at: 4557 4557
Size: 6958
MluI A'CGCG_T
Cuts at: 6834 6834
Size: 6958
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2014 4199 6072 6256 1305
Size: 709 2185 1873 184 2007
Fragments arranged by size:
2185 2007 1873 709 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2278 3612 10
Size: 54 1425 343 446 1334 3356
Fragments arranged by size:
3356 1425 1334 446 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2337 2385 2910 3552 4916 5275 5434
Size: 648 1171 48 525 642 1364 359 159
Cuts at: 5434 518
Size: 2042
Fragments arranged by size:
2042 1364 1171 648 642 525 359 159
48
MspA1I CmG'CkG
Cuts at: 1606 3091 3543 3731 4374 5051 6199 6444 1606
Size: 1485 452 188 643 677 1148 245 2120
Fragments arranged by size:
2120 1485 1148 677 643 452 245 188
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5467
Fragments arranged by size:
5467 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6958
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6958
NotI GC'GGCC_GC
Cuts at: 4405 4405
Size: 6958
NspI r_CATG'y
Cuts at: 1132 1229 2687 4316 5861 1132
Size: 97 1458 1629 1545 2229
Fragments arranged by size:
2229 1629 1545 1458 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6958
PciI A'CATG_T
Cuts at: 2683 5857 2683
Size: 3174 3784
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6958
PfoI T'CCnGG_A
Cuts at: 3139 3139
Size: 6958
PmeI GTTT'AAAC
Cuts at: 4262 4262
Size: 6958
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5027 5059 6540 6572 5027
Size: 32 1481 32 5413
Fragments arranged by size:
5413 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3589 3589
Size: 6958
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6958
PsiI TTA'TAA
Cuts at: 4528 4528
Size: 6958
PspOMI G'GGCC_C
Cuts at: 3077 3077
Size: 6958
PstI C_TGCA'G
Cuts at: 830 4310 830
Size: 3480 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6958
PvuII CAG'CTG
Cuts at: 3091 3731 3091
Size: 640 6318
SacI G_AGCT'C
Cuts at: 3420 3420
Size: 6958
SalI G'TCGA_C
Cuts at: 1936 4300 1936
Size: 2364 4594
SanDI GG'GwC_CC
Cuts at: 3589 3589
Size: 6958
SbfI CC_TGCA'GG
Cuts at: 4310 4310
Size: 6958
ScaI AGT'ACT
Cuts at: 5206 6918 5206
Size: 1712 5246
SfcI C'TryA_G
Cuts at: 826 1045 2164 3939 4306 5441 6122 6313 826
Size: 219 1119 1775 367 1135 681 191 1471
Fragments arranged by size:
1775 1471 1135 1119 681 367 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6958
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6958
SmaI CCC'GGG
Cuts at: 1872 4285 1872
Size: 2413 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5062 5963 6225 820
Size: 197 926 3119 901 262 1553
Fragments arranged by size:
3119 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4749 493
Size: 4256 2702
SpeI A'CTAG_T
Cuts at: 152 2962 152
Size: 2810 4148
SphI G_CATG'C
Cuts at: 1132 4316 1132
Size: 3184 3774
SspI AAT'ATT
Cuts at: 5 52 2239 4882 5
Size: 47 2187 2643 2081
Fragments arranged by size:
2643 2187 2081 47
StuI AGG'CCT
Cuts at: 1517 3256 1517
Size: 1739 5219
StyI C'CwwG_G
Cuts at: 513 1065 2004 3174 3916 4397 513
Size: 552 939 1170 742 481 3074
Fragments arranged by size:
3074 1170 939 742 552 481
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3172 4985 5002 5155 5340 5785 325
Size: 1255 1592 1813 17 153 185 445 1498
Fragments arranged by size:
1813 1592 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2420 2724 3225
Size: 274 80 33 51 1443 443 304 501
Cuts at: 3225 5204 6916 96
Size: 1979 1712 138
Fragments arranged by size:
1979 1712 1443 501 443 304 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2213 2245 2673 2705 2997 3029 3870 3902 6918
Size: 32 428 32 292 32 841 32 3016
Cuts at: 6918 6950 2213
Size: 32 2221
Fragments arranged by size:
3016 2221 841 428 292 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6958
XbaI T'CTAG_A
Cuts at: 4294 4294
Size: 6958
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6958
XmaI C'CCGG_G
Cuts at: 1870 4283 1870
Size: 2413 4545
XmnI GAAnn'nnTTC
Cuts at: 5087 5087
Size: 6958
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4766 276
Size: 53 83 186 936 3232 2468
Fragments arranged by size:
3232 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI Bsu36I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI
SfoI SrfI SwaI XcmI