Restriction map for
pFN21AB7019
(Linear) (Six-base) MAPSORT of: KIBB7019.seq Check: 6521 from: 1 to: 2253
KIBB7019.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AccI GT'mk_AC
Cuts at: 0 1285 2253
Size: 1285 968
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 2253
Size: 117 2136
AflIII A'CryG_T
Cuts at: 0 678 2253
Size: 678 1575
AhdI GACnn_n'nnGTC
Cuts at: 0 2019 2253
Size: 2019 234
AleI CACnn'nnGTG
Cuts at: 0 332 2253
Size: 332 1921
AlwNI CAG_nnn'CTG
Cuts at: 0 586 2253
Size: 586 1667
ApaI G_GGCC'C
Cuts at: 0 1076 2253
Size: 1076 1177
ApoI r'AATT_y
Cuts at: 0 1106 1469 2253
Size: 1106 363 784
Fragments arranged by size:
1106 784 363
AseI AT'TA_AT
Cuts at: 0 172 2253
Size: 172 2081
AvaI C'yCGr_G
Cuts at: 0 945 2253
Size: 945 1308
BanII G_rGCy'C
Cuts at: 0 1076 1415 2253
Size: 1076 339 838
Fragments arranged by size:
1076 838 339
BbsI GAAGACnn'nnnn_
Cuts at: 0 890 1659 2253
Size: 890 769 594
Fragments arranged by size:
890 769 594
BclI T'GATC_A
Cuts at: 0 533 630 2253
Size: 533 97 1623
Fragments arranged by size:
1623 533 97
Bme1580I G_kGCm'C
Cuts at: 0 1076 1779 2253
Size: 1076 703 474
Fragments arranged by size:
1076 703 474
BmrI ACTGGGnnnn_n'
Cuts at: 0 1643 2253
Size: 1643 610
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 567 2253
Size: 567 1686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1441 1789 2253
Size: 1441 348 464
Fragments arranged by size:
1441 464 348
Bpu10I CC'TnA_GC
Cuts at: 0 713 2253
Size: 713 1540
BsaBI GATnn'nnATC
Cuts at: 0 182 2253
Size: 182 2071
BsaWI w'CCGG_w
Cuts at: 0 8 191 2253
Size: 8 183 2062
Fragments arranged by size:
2062 183 8
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1865 1895 2253
Size: 1865 30 358
Fragments arranged by size:
1865 358 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 450 556 1154 1438 1659 2253
Size: 450 106 598 284 221 594
Fragments arranged by size:
598 594 450 284 221 106
BseYI C'CCAG_C
Cuts at: 0 68 2253
Size: 68 2185
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1516 1756 2253
Size: 1516 240 497
Fragments arranged by size:
1516 497 240
BsiHKAI G_wGCw'C
Cuts at: 0 763 871 1415 2253
Size: 763 108 544 838
Fragments arranged by size:
838 763 544 108
BsmI GAATG_Cn'
Cuts at: 0 4 304 621 2086 2253
Size: 4 300 317 1465 167
Fragments arranged by size:
1465 317 300 167 4
Bsp1286I G_dGCh'C
Cuts at: 0 763 871 1076 1415 1779 2253
Size: 763 108 205 339 364 474
Fragments arranged by size:
763 474 364 339 205 108
BspEI T'CCGG_A
Cuts at: 0 8 2253
Size: 8 2245
BspHI T'CATG_A
Cuts at: 0 458 2253
Size: 458 1795
BsrBI CCG'CTC
Cuts at: 0 1892 2253
Size: 1892 361
BsrDI GCAATG_nn'
Cuts at: 0 1928 2253
Size: 1928 325
BsrGI T'GTAC_A
Cuts at: 0 1220 2253
Size: 1220 1033
BstBI TT'CG_AA
Cuts at: 0 597 2253
Size: 597 1656
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1549 1767 2253
Size: 1549 218 486
Fragments arranged by size:
1549 486 218
BstYI r'GATC_y
Cuts at: 0 61 318 2253
Size: 61 257 1935
Fragments arranged by size:
1935 257 61
BstZ17I GTA'TAC
Cuts at: 0 1286 2253
Size: 1286 967
BtgI C'CryG_G
Cuts at: 0 2026 2253
Size: 2026 227
BtsI GCAGTG_nn'
Cuts at: 0 1923 2253
Size: 1923 330
EaeI y'GGCC_r
Cuts at: 0 271 1605 2253
Size: 271 1334 648
Fragments arranged by size:
1334 648 271
EcoICRI GAG'CTC
Cuts at: 0 1413 2253
Size: 1413 840
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 1441 1789 2253
Size: 117 1324 348 464
Fragments arranged by size:
1324 464 348 117
EcoO109I rG'GnC_Cy
Cuts at: 0 1072 1584 2199 2253
Size: 1072 512 615 54
Fragments arranged by size:
1072 615 512 54
EcoRI G'AATT_C
Cuts at: 0 1106 2253
Size: 1106 1147
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 738 1989 2253
Size: 738 1251 264
Fragments arranged by size:
1251 738 264
HaeII r_GCGC'y
Cuts at: 0 401 2253
Size: 401 1852
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 351 383 1080 1112 1319 1351 1871 1903
Size: 351 32 697 32 207 32 520 32
Cuts at: 1903 2005 2037 2253
Size: 102 32 216
Fragments arranged by size:
697 520 351 216 207 102 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 831 2253
Size: 831 1422
HindIII A'AGCT_T
Cuts at: 0 254 2253
Size: 254 1999
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 9 2194 2253
Size: 9 2185 59
Fragments arranged by size:
2185 59 9
MscI TGG'CCA
Cuts at: 0 273 1607 2253
Size: 273 1334 646
Fragments arranged by size:
1334 646 273
MslI CAynn'nnrTG
Cuts at: 0 332 380 905 1547 2253
Size: 332 48 525 642 706
Fragments arranged by size:
706 642 525 332 48
MspA1I CmG'CkG
Cuts at: 0 1086 1538 1726 2253
Size: 1086 452 188 527
Fragments arranged by size:
1086 527 452 188
NspI r_CATG'y
Cuts at: 0 682 2253
Size: 682 1571
PciI A'CATG_T
Cuts at: 0 678 2253
Size: 678 1575
PfoI T'CCnGG_A
Cuts at: 0 1134 2253
Size: 1134 1119
PpuMI rG'GwC_Cy
Cuts at: 0 1584 2253
Size: 1584 669
PspOMI G'GGCC_C
Cuts at: 0 1072 2253
Size: 1072 1181
PvuII CAG'CTG
Cuts at: 0 1086 1726 2253
Size: 1086 640 527
Fragments arranged by size:
1086 640 527
SacI G_AGCT'C
Cuts at: 0 1415 2253
Size: 1415 838
SanDI GG'GwC_CC
Cuts at: 0 1584 2253
Size: 1584 669
SfcI C'TryA_G
Cuts at: 0 159 1934 2253
Size: 159 1775 319
Fragments arranged by size:
1775 319 159
SpeI A'CTAG_T
Cuts at: 0 957 2253
Size: 957 1296
SspI AAT'ATT
Cuts at: 0 234 2253
Size: 234 2019
StuI AGG'CCT
Cuts at: 0 1251 2253
Size: 1251 1002
StyI C'CwwG_G
Cuts at: 0 1169 1911 2253
Size: 1169 742 342
Fragments arranged by size:
1169 742 342
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1167 2253
Size: 1167 1086
TatI w'GTAC_w
Cuts at: 0 415 719 1220 2253
Size: 415 304 501 1033
Fragments arranged by size:
1033 501 415 304
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 208 240 668 700 992 1024 1865 1897
Size: 208 32 428 32 292 32 841 32
Cuts at: 1897 2253
Size: 356
Fragments arranged by size:
841 428 356 292 208 32 32 32
32
Enzymes that do cut:
AccI AcuI AflIII AhdI AleI AlwNI ApaI ApoI
AseI AvaI BanII BbsI BclI Bme1580I BmrI BplI
BpmI Bpu10I BsaBI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspEI BspHI BsrBI BsrDI BsrGI
BstBI BstXI BstYI BstZ17I BtgI BtsI EaeI EcoICRI
Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII
MmeI MscI MslI MspA1I NspI PciI PfoI PpuMI
PspOMI PvuII SacI SanDI SfcI SpeI SspI StuI
StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AlfI
AloI ApaLI AscI AsiSI AvrII BaeI BamHI BanI
BbeI BbvCI BcgI BciVI BfrBI BglI BglII BlpI
BmgBI BmtI BpuEI BsaI BsaAI BsaHI BsiEI BsiWI
BsmBI BspMI BsrFI BssHII BssSI BstAPI BstEII Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI
EciI EcoNI EcoRV FseI FspI FspAI HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SphI SrfI
SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI