(Circular) (Six-base) MAPSORT of: pFN21AB7008.seq Check: 5944 from: 1 to: 6100 pFN21AB7008 6100 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2551 3034 3171 2551 Size: 483 137 5480 Fragments arranged by size: 5480 483 137 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2337 3910 278 Size: 53 83 186 936 801 1573 2468 Fragments arranged by size: 2468 1573 936 801 186 83 53 AccI GT'mk_AC Cuts at: 1937 3443 1937 Size: 1506 4594 Acc65I G'GTAC_C Cuts at: 3421 3421 Size: 6100 AclI AA'CG_TT Cuts at: 1563 4227 4600 1563 Size: 2664 373 3063 Fragments arranged by size: 3063 2664 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2093 2210 4163 5547 5815 1632 Size: 461 117 1953 1384 268 1917 Fragments arranged by size: 1953 1917 1384 461 268 117 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5903 AflIII A'CryG_T Cuts at: 1203 4999 5976 1203 Size: 3796 977 1327 Fragments arranged by size: 3796 1327 977 AgeI A'CCGG_T Cuts at: 4931 4931 Size: 6100 AhdI GACnn_n'nnGTC Cuts at: 4829 4829 Size: 6100 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6100 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2742 1184 Size: 1558 4542 AlwNI CAG_nnn'CTG Cuts at: 2635 3843 5415 2635 Size: 1208 1572 3320 Fragments arranged by size: 3320 1572 1208 ApaLI G'TGCA_C Cuts at: 2374 4157 5313 2374 Size: 1783 1156 3161 Fragments arranged by size: 3161 1783 1156 ApoI r'AATT_y Cuts at: 1493 3409 3635 3787 5896 1493 Size: 1916 226 152 2109 1697 Fragments arranged by size: 2109 1916 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4654 160 Size: 872 3622 1606 Fragments arranged by size: 3622 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 AvaI C'yCGr_G Cuts at: 1870 1943 2047 2175 3425 1870 Size: 73 104 128 1250 4545 Fragments arranged by size: 4545 1250 128 104 73 BamHI G'GATC_C Cuts at: 3430 3797 3430 Size: 367 5733 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2399 2492 2857 3421 4876 Size: 325 217 627 612 93 365 564 1455 Cuts at: 4876 618 Size: 1842 Fragments arranged by size: 1842 1455 627 612 564 365 325 217 93 BanII G_rGCy'C Cuts at: 1391 2355 2936 3419 1391 Size: 964 581 483 4072 Fragments arranged by size: 4072 964 581 483 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2225 2289 928 Size: 971 326 64 4739 Fragments arranged by size: 4739 971 326 64 BbvCI CC'TCA_GC Cuts at: 2442 2681 2442 Size: 239 5861 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4291 4325 1903 Size: 34 2354 34 3678 Fragments arranged by size: 3678 2354 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2910 3992 5202 2910 Size: 1082 1210 3808 Fragments arranged by size: 3808 1210 1082 BclI T'GATC_A Cuts at: 1548 1548 Size: 6100 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2192 2453 2699 Size: 107 122 71 631 605 520 261 246 Cuts at: 2699 2825 2875 3138 3320 4711 136 Size: 126 50 263 182 1391 1525 Fragments arranged by size: 1525 1391 631 605 520 263 261 246 182 126 122 107 71 50 BglII A'GATC_T Cuts at: 6095 6095 Size: 6100 BlpI GC'TnA_GC Cuts at: 3517 3517 Size: 6100 Bme1580I G_kGCm'C Cuts at: 1790 2176 2378 2862 3307 4161 5317 1790 Size: 386 202 484 445 854 1156 2573 Fragments arranged by size: 2573 1156 854 484 445 386 202 BmgBI CAC'GTC Cuts at: 1318 3220 1318 Size: 1902 4198 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2566 2883 3831 Size: 416 53 83 399 24 1149 317 948 Cuts at: 3831 4789 442 Size: 958 1753 Fragments arranged by size: 1753 1149 958 948 416 399 317 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6100 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2078 2585 3027 3179 4760 1239 Size: 839 507 442 152 1581 2579 Fragments arranged by size: 2579 1581 839 507 442 152 Bpu10I CC'TnA_GC Cuts at: 2442 2681 2442 Size: 239 5861 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4225 5090 5388 4225 Size: 865 298 4937 Fragments arranged by size: 4937 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2549 4763 882 Size: 617 1050 2214 2219 Fragments arranged by size: 2219 2214 1050 617 BsaAI yAC'GTr Cuts at: 493 1204 3891 493 Size: 711 2687 2702 Fragments arranged by size: 2702 2687 711 BsaBI GATnn'nnATC Cuts at: 6094 6094 Size: 6100 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2334 2838 3907 4289 Size: 53 83 186 936 801 504 1069 382 Cuts at: 4289 4924 275 Size: 635 1451 Fragments arranged by size: 1451 1069 936 801 635 504 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2286 2337 4533 4931 5205 5352 5763 2286 Size: 51 2196 398 274 147 411 2623 Fragments arranged by size: 2623 2196 411 398 274 147 51 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5875 Fragments arranged by size: 5875 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2075 2292 1186 Size: 889 217 4994 Fragments arranged by size: 4994 889 217 BseYI C'CCAG_C Cuts at: 2865 2923 3001 3057 5303 5799 2865 Size: 58 78 56 2246 496 3166 Fragments arranged by size: 3166 2246 496 78 58 56 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2126 2192 2729 1163 Size: 573 390 66 537 4534 Fragments arranged by size: 4534 573 537 390 66 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3550 4311 5339 1540 Size: 275 186 1549 761 1028 2301 Fragments arranged by size: 2301 1549 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2378 2385 4161 4246 5317 2378 Size: 7 1776 85 1071 3161 Fragments arranged by size: 3161 1776 1071 85 7 BsmI GAATG_Cn' Cuts at: 3611 3704 3611 Size: 93 6007 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2176 2355 2378 2385 2862 2936 3307 Size: 399 386 179 23 7 477 74 371 Cuts at: 3307 3419 4161 4246 5317 1391 Size: 112 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 477 399 386 371 179 112 85 74 23 7 BspEI T'CCGG_A Cuts at: 2337 5763 2337 Size: 3426 2674 BspHI T'CATG_A Cuts at: 3989 5719 3989 Size: 1730 4370 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2551 3034 3171 3455 844 Size: 1707 483 137 284 3489 Fragments arranged by size: 3489 1707 483 284 137 BsrBI CCG'CTC Cuts at: 1394 1823 3987 1394 Size: 429 2164 3507 Fragments arranged by size: 3507 2164 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4595 4769 65 Size: 1183 3347 174 1396 Fragments arranged by size: 3347 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3390 4744 4931 1700 Size: 110 1580 1354 187 2869 Fragments arranged by size: 2869 1580 1354 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6100 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6100 BssSI C'ACGA_G Cuts at: 4160 5172 4160 Size: 1012 5088 BstAPI GCAn_nnn'nTGC Cuts at: 794 2412 794 Size: 1618 4482 BstBI TT'CG_AA Cuts at: 2085 4904 2085 Size: 2819 3281 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6100 BstXI CCAn_nnnn'nTGG Cuts at: 2509 2575 2873 2509 Size: 66 298 5736 Fragments arranged by size: 5736 298 66 BstYI r'GATC_y Cuts at: 1972 2043 2100 3189 3430 3797 4182 4199 5651 Size: 71 57 1089 241 367 385 17 1452 Cuts at: 5651 5737 5749 6095 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1089 385 367 346 241 86 71 57 17 12 Bsu36I CC'TnA_GG Cuts at: 2773 3378 2773 Size: 605 5495 BtgI C'CryG_G Cuts at: 513 1065 2688 2697 2937 2946 513 Size: 552 1623 9 240 9 3667 Fragments arranged by size: 3667 1623 552 240 9 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2578 2734 2782 487 Size: 683 56 1352 156 48 3805 Fragments arranged by size: 3805 1352 683 156 56 48 BtsI GCAGTG_nn' Cuts at: 3616 4410 4430 3616 Size: 794 20 5286 Fragments arranged by size: 5286 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6100 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6065 DraI TTT'AAA Cuts at: 3404 3404 Size: 6100 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5107 809 Size: 507 3791 1802 Fragments arranged by size: 3791 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2454 2565 2691 Size: 54 1425 50 275 18 624 111 126 Cuts at: 2691 3214 3297 3547 4436 6065 8 Size: 523 83 250 889 1629 43 Fragments arranged by size: 1629 1425 889 624 523 275 250 126 111 83 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3547 1537 Size: 275 1735 4090 Fragments arranged by size: 4090 1735 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4030 1353 Size: 2677 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2537 4687 5061 5207 387 Size: 1064 138 948 2150 374 146 1280 Fragments arranged by size: 2150 1280 1064 948 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2078 2093 2210 2585 3027 3179 4163 Size: 393 446 15 117 375 442 152 984 Cuts at: 4163 4760 5547 5815 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 984 787 597 446 442 393 375 268 152 117 15 EcoNI CCTnn'n_nnAGG Cuts at: 2960 3167 2960 Size: 207 5893 EcoO109I rG'GnC_Cy Cuts at: 2068 2632 3131 2068 Size: 564 499 5037 Fragments arranged by size: 5037 564 499 EcoRI G'AATT_C Cuts at: 3409 3787 3409 Size: 378 5722 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2098 2098 Size: 6100 FspI TGC'GCA Cuts at: 4606 4606 Size: 6100 HaeII r_GCGC'y Cuts at: 1678 2205 2622 3355 5247 1678 Size: 527 417 733 1892 2531 Fragments arranged by size: 2531 1892 733 527 417 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4741 4773 4815 4847 1373 Size: 32 135 32 3169 32 42 32 2626 Fragments arranged by size: 3169 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2842 3244 3444 3690 669 Size: 988 281 904 402 200 246 3079 Fragments arranged by size: 3079 988 904 402 281 246 200 HindIII A'AGCT_T Cuts at: 748 748 Size: 6100 HpaI GTT'AAC Cuts at: 3690 3690 Size: 6100 KpnI G_GTAC'C Cuts at: 3425 3425 Size: 6100 MfeI C'AATT_G Cuts at: 3699 3699 Size: 6100 MluI A'CGCG_T Cuts at: 5976 5976 Size: 6100 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2078 5214 5398 1305 Size: 773 3136 184 2007 Fragments arranged by size: 3136 2007 773 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2567 2693 3216 10 Size: 54 1425 343 735 126 523 2894 Fragments arranged by size: 2894 1425 735 523 343 126 54 MslI CAynn'nnrTG Cuts at: 518 1166 4058 4417 4576 518 Size: 648 2892 359 159 2042 Fragments arranged by size: 2892 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2939 3516 4193 5341 5586 1606 Size: 1333 577 677 1148 245 2120 Fragments arranged by size: 2120 1333 1148 677 577 245 NcoI C'CATG_G Cuts at: 513 1065 2688 2697 513 Size: 552 1623 9 3916 Fragments arranged by size: 3916 1623 552 9 NdeI CA'TA_TG Cuts at: 387 387 Size: 6100 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6100 NotI GC'GGCC_GC Cuts at: 3547 3547 Size: 6100 NspI r_CATG'y Cuts at: 1132 1229 2557 2584 3458 5003 1132 Size: 97 1328 27 874 1545 2229 Fragments arranged by size: 2229 1545 1328 874 97 27 PasI CC'CwG_GG Cuts at: 1348 2833 1348 Size: 1485 4615 PciI A'CATG_T Cuts at: 4999 4999 Size: 6100 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6100 PmeI GTTT'AAAC Cuts at: 3404 3404 Size: 6100 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4169 4201 5682 5714 4169 Size: 32 1481 32 4555 Fragments arranged by size: 4555 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2068 2068 Size: 6100 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6100 PsiI TTA'TAA Cuts at: 3670 3670 Size: 6100 PstI C_TGCA'G Cuts at: 830 2961 3452 830 Size: 2131 491 3478 Fragments arranged by size: 3478 2131 491 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6100 SacII CC_GC'GG Cuts at: 2940 2940 Size: 6100 SalI G'TCGA_C Cuts at: 1936 3442 1936 Size: 1506 4594 SbfI CC_TGCA'GG Cuts at: 3452 3452 Size: 6100 ScaI AGT'ACT Cuts at: 4348 6060 4348 Size: 1712 4388 SfcI C'TryA_G Cuts at: 826 1045 2614 2848 2957 3010 3448 4583 5264 Size: 219 1569 234 109 53 438 1135 681 Cuts at: 5264 5455 826 Size: 191 1471 Fragments arranged by size: 1569 1471 1135 681 438 234 219 191 109 53 SfiI GGCCn_nnn'nGGCC Cuts at: 2453 2453 Size: 6100 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6100 SmaI CCC'GGG Cuts at: 1872 2177 3427 1872 Size: 305 1250 4545 Fragments arranged by size: 4545 1250 305 SmlI C'TyrA_G Cuts at: 820 1017 1943 4204 5105 5367 820 Size: 197 926 2261 901 262 1553 Fragments arranged by size: 2261 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3891 493 Size: 3398 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6100 SphI G_CATG'C Cuts at: 1132 2557 3458 1132 Size: 1425 901 3774 Fragments arranged by size: 3774 1425 901 SspI AAT'ATT Cuts at: 5 52 4024 5 Size: 47 3972 2081 Fragments arranged by size: 3972 2081 47 StuI AGG'CCT Cuts at: 1517 2966 1517 Size: 1449 4651 StyI C'CwwG_G Cuts at: 513 1065 2688 2697 3539 513 Size: 552 1623 9 842 3074 Fragments arranged by size: 3074 1623 842 552 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2585 2642 2879 3261 4127 4144 4297 Size: 1255 1005 57 237 382 866 17 153 Cuts at: 4297 4482 4927 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1005 866 445 382 237 185 153 57 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2130 4346 6058 Size: 274 80 33 51 1443 153 2216 1712 Cuts at: 6058 96 Size: 138 Fragments arranged by size: 2216 1712 1443 274 153 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3015 3047 6060 6092 3015 Size: 32 3013 32 3023 Fragments arranged by size: 3023 3013 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6100 XbaI T'CTAG_A Cuts at: 3436 3436 Size: 6100 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2695 3326 2695 Size: 631 5469 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6100 XmaI C'CCGG_G Cuts at: 1870 2175 3425 1870 Size: 305 1250 4545 Fragments arranged by size: 4545 1250 305 XmnI GAAnn'nnTTC Cuts at: 3332 4229 3332 Size: 897 5203 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2335 3908 276 Size: 53 83 186 936 801 1573 2468 Fragments arranged by size: 2468 1573 936 801 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfoI SrfI SwaI