Restriction map for
pFN21AB6003
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# NO CUT ENZYMES ( 86 enzymes) #
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Acc65I AccII AgeI AhdI Aor51HI
ApaI AscI Asp718I AspEI AviII
AvrII BbeI BbrPI BcgI BciVI
BclI BlnI BsaBI BsaI BsiEI
BsiWI BsmBI BspDI BspLU11I BsrBI
BssHII BstAPI BstBI BstUI ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I FauI FbaI
FseI FspI KasI KpnI KspI
MamI MfeI MluI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 3207 GACGTC GACGT^C
AccIII 1575 TCCGGA T^CCGGA
AclI 808 AACGTT AA^CGTT
AflII 1170 CTTAAG C^TTAAG
AflIII 2700 ACGTGT A^C[GA][CT]GT
Alw44I 2549 GTGCAC G^TGCAC
AocI 359 CCTTAGG CC^T[ACGT]AGG
ApaLI 2549 GTGCAC G^TGCAC
Asp700I 3429 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 3517 CCCGAG C^[CT]CG[GA]G
BamHI 1595 GGATCC G^GATCC
BanII 2338 GAGCCC G[GA]GC[CT]^C
BbvCI 66 CCTCAGC CCTCAGC(-5/-2)
BcnI 2874 CCCGG CC^[GC]GG
BfrI 1170 CTTAAG C^TTAAG
BglI 3391 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1057 AGATCT A^GATCT
BlpI 1048 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1048 GCTCAGC GC^T[ACGT]AGC
BsaAI 3454 TACGTG [CT]AC^GT[GA]
BsaWI 1575 TCCGGA [AT]^CCGG[AT]
BseAI 1575 TCCGGA T^CCGGA
BsmI 2881 GAATGC GAATGC(1/-1)
BsoBI 3517 CCCGAG C^[CT]CG[GA]G
BspEI 1575 TCCGGA T^CCGGA
BspMI 156 GCAGGT ACCTGC(4/8)
BsrFI 26 GCCGGT [GA]^CCGG[CT]
Bst1107I 506 GTATAC GTA^TAC
BstEII 634 GGTCACC G^GT[ACGT]ACC
BstPI 634 GGTCACC G^GT[ACGT]ACC
BstZ17I 506 GTATAC GTA^TAC
Bsu36I 359 CCTTAGG CC^T[ACGT]AGG
CelII 1048 GCTCAGC GC^T[ACGT]AGC
Cfr10I 26 GCCGGT [GA]^CCGG[CT]
DraIII 937 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3395 CCATGG C^C[GA][CT]GG
Eco81I 359 CCTTAGG CC^T[ACGT]AGG
EcoO65I 634 GGTCACC G^GT[ACGT]ACC
HaeII 3584 GGCGCT [GA]GCGC^[CT]
MroI 1575 TCCGGA T^CCGGA
NciI 2874 CCCGG CC^[GC]GG
NcoI 3395 CCATGG C^CATGG
Psp1406I 808 AACGTT AA^CGTT
PstI 2649 CTGCAG CTGCA^G
SapI 2335 GAAGAGC GCTCTTC(1/4)
SphI 1005 GCATGC GCATG^C
SspI 2297 AATATT AAT^ATT
TaqI 3275 TCGA T^CGA
TthHB8I 3275 TCGA T^CGA
XmnI 3429 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccI 506 GTATAC GT^[AC][GT]AC
3451 GTCTAC
AspI 193 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3376 GACGCCGTC
BanI 354 GGTGCC G^G[CT][GA]CC
3172 GGCACC
BstXI 3054 CCACTTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3185 CCAAAGTTTTGG
CfoI 3304 GCGC GCG^C
3585 GCGC
EcoNI 2530 CCTTTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3282 CCTCCGCCAGG
EcoRI 2385 GAATTC G^AATTC
2463 GAATTC
EcoRV 1303 GATATC GAT^ATC
1399 GATATC
EcoT22I 1469 ATGCAT ATGCA^T
3040 ATGCAT
HhaI 3304 GCGC GCG^C
3585 GCGC
HinP1I 3304 GCGC G^CGC
3585 GCGC
HindIII 2039 AAGCTT A^AGCTT
3508 AAGCTT
HpaI 226 GTTAAC GTT^AAC
3108 GTTAAC
MspA1I 2196 CCGCTG C[AC]G^C[GT]G
2895 CCGCTG
NsiI 1469 ATGCAT ATGCA^T
3040 ATGCAT
NspI 510 ACATGC [GA]CATG^[CT]
1005 GCATGC
PflFI 193 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3376 GACGCCGTC
PshAI 412 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
628 GACATAGGTC
Tth111I 193 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3376 GACGCCGTC
XbaI 489 TCTAGA T^CTAGA
2574 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
AspHI AvaII BalI BbsI BbvI
BfaI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspHI BsrDI BsrGI BsrI BssKI
BssSI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI Hin1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PshBI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XhoII