(Circular) (Six-base) MAPSORT of: pFN21AB5678.seq Check: 1881 from: 1 to: 7039 pFN21AB5678 7039 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4849 278 Size: 53 83 186 936 3313 2468 Fragments arranged by size: 3313 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4382 1937 Size: 2445 4594 Acc65I G'GTAC_C Cuts at: 4360 4360 Size: 7039 AclI AA'CG_TT Cuts at: 1563 5166 5539 1563 Size: 3603 373 3063 Fragments arranged by size: 3603 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3401 4089 5102 6486 6754 1632 Size: 1769 688 1013 1384 268 1917 Fragments arranged by size: 1917 1769 1384 1013 688 268 AfeI AGC'GCT Cuts at: 3485 3485 Size: 7039 AflII C'TTAA_G Cuts at: 820 1017 3455 820 Size: 197 2438 4404 Fragments arranged by size: 4404 2438 197 AflIII A'CryG_T Cuts at: 1203 5938 6915 1203 Size: 4735 977 1327 Fragments arranged by size: 4735 1327 977 AgeI A'CCGG_T Cuts at: 5870 5870 Size: 7039 AhdI GACnn_n'nnGTC Cuts at: 5768 5768 Size: 7039 AleI CACnn'nnGTG Cuts at: 1166 3470 1166 Size: 2304 4735 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7039 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3881 3913 3881 Size: 32 7007 AlwNI CAG_nnn'CTG Cuts at: 2213 2716 3328 3389 4782 6354 2213 Size: 503 612 61 1393 1572 2898 Fragments arranged by size: 2898 1572 1393 612 503 61 ApaI G_GGCC'C Cuts at: 2474 2809 4116 2474 Size: 335 1307 5397 Fragments arranged by size: 5397 1307 335 ApaLI G'TGCA_C Cuts at: 5096 6252 5096 Size: 1156 5883 ApoI r'AATT_y Cuts at: 1493 3572 4191 4348 4574 4726 6835 1493 Size: 2079 619 157 226 152 2109 1697 Fragments arranged by size: 2109 2079 1697 619 226 157 152 AseI AT'TA_AT Cuts at: 160 1032 2760 5593 160 Size: 872 1728 2833 1606 Fragments arranged by size: 2833 1728 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7039 AvaI C'yCGr_G Cuts at: 1870 1943 3399 3635 4062 4364 1870 Size: 73 1456 236 427 302 4545 Fragments arranged by size: 4545 1456 427 302 236 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2638 2671 2638 Size: 33 7006 BamHI G'GATC_C Cuts at: 3288 4369 4736 3288 Size: 1081 367 5591 Fragments arranged by size: 5591 1081 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3472 4313 4360 5815 618 Size: 325 217 627 1685 841 47 1455 1842 Fragments arranged by size: 1842 1685 1455 841 627 325 217 47 BanII G_rGCy'C Cuts at: 1391 2474 2809 2972 4116 4358 1391 Size: 1083 335 163 1144 242 4072 Fragments arranged by size: 4072 1144 1083 335 242 163 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2073 928 Size: 971 174 5894 Fragments arranged by size: 5894 971 174 BbvCI CC'TCA_GC Cuts at: 2052 4050 2052 Size: 1998 5041 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5230 5264 1903 Size: 34 3293 34 3678 Fragments arranged by size: 3678 3293 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4931 6141 4931 Size: 1210 5829 BclI T'GATC_A Cuts at: 1548 1548 Size: 7039 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2040 2444 2618 Size: 107 122 71 631 605 368 404 174 Cuts at: 2618 2813 3740 5650 136 Size: 195 927 1910 1525 Fragments arranged by size: 1910 1525 927 631 605 404 368 195 174 122 107 71 BglII A'GATC_T Cuts at: 2109 3808 7034 2109 Size: 1699 3226 2114 Fragments arranged by size: 3226 2114 1699 BlpI GC'TnA_GC Cuts at: 4456 4456 Size: 7039 Bme1580I G_kGCm'C Cuts at: 1790 2316 2474 2809 3477 3873 4116 5100 6256 Size: 526 158 335 668 396 243 984 1156 Cuts at: 6256 1790 Size: 2573 Fragments arranged by size: 2573 1156 984 668 526 396 335 243 158 BmgBI CAC'GTC Cuts at: 1318 2963 1318 Size: 1645 5394 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3002 3020 3038 Size: 416 53 83 399 24 1585 18 18 Cuts at: 3038 3056 3665 4770 5728 442 Size: 18 609 1105 958 1753 Fragments arranged by size: 1753 1585 1105 958 609 416 399 83 53 24 18 18 18 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7039 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2738 2848 2958 3174 4138 4219 5699 1239 Size: 1499 110 110 216 964 81 1480 2579 Fragments arranged by size: 2579 1499 1480 964 216 110 110 81 Bpu10I CC'TnA_GC Cuts at: 2052 4050 2052 Size: 1998 5041 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5164 6029 6327 5164 Size: 865 298 5876 Fragments arranged by size: 5876 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2636 3692 3969 5702 882 Size: 617 1137 1056 277 1733 2219 Fragments arranged by size: 2219 1733 1137 1056 617 277 BsaAI yAC'GTr Cuts at: 493 1204 4830 493 Size: 711 3626 2702 Fragments arranged by size: 3626 2702 711 BsaBI GATnn'nnATC Cuts at: 3528 7033 3528 Size: 3505 3534 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2897 4846 5228 5863 Size: 53 83 186 936 1364 1949 382 635 Cuts at: 5863 275 Size: 1451 Fragments arranged by size: 1949 1451 1364 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5472 5870 6144 6291 6702 5472 Size: 398 274 147 411 5809 Fragments arranged by size: 5809 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3783 3813 3881 3911 1178 Size: 30 165 30 2380 30 68 30 4306 Fragments arranged by size: 4306 2380 165 68 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 3680 1186 Size: 841 1653 4545 Fragments arranged by size: 4545 1653 841 BseYI C'CCAG_C Cuts at: 4122 6242 6738 4122 Size: 2120 496 4423 Fragments arranged by size: 4423 2120 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2664 2820 3375 3978 1163 Size: 573 928 156 555 603 4224 Fragments arranged by size: 4224 928 603 573 555 156 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4489 5250 6278 1540 Size: 275 186 2488 761 1028 2301 Fragments arranged by size: 2488 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2889 5100 5185 6256 2889 Size: 2211 85 1071 3672 Fragments arranged by size: 3672 2211 1071 85 BsmI GAATG_Cn' Cuts at: 2254 2458 4231 4550 4643 2254 Size: 204 1773 319 93 4650 Fragments arranged by size: 4650 1773 319 204 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2316 2474 2809 2889 2972 3477 3873 Size: 399 526 158 335 80 83 505 396 Cuts at: 3873 4116 4358 5100 5185 6256 1391 Size: 243 242 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 526 505 399 396 335 243 242 158 85 83 80 BspEI T'CCGG_A Cuts at: 6702 6702 Size: 7039 BspHI T'CATG_A Cuts at: 3717 4928 6658 3717 Size: 1211 1730 4098 Fragments arranged by size: 4098 1730 1211 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3336 3354 4394 844 Size: 2492 18 1040 3489 Fragments arranged by size: 3489 2492 1040 18 BsrBI CCG'CTC Cuts at: 1394 1823 4233 4926 1394 Size: 429 2410 693 3507 Fragments arranged by size: 3507 2410 693 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3954 5534 5708 65 Size: 1183 2706 1580 174 1396 Fragments arranged by size: 2706 1580 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4256 5683 5870 1700 Size: 110 2446 1427 187 2869 Fragments arranged by size: 2869 2446 1427 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7039 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7039 BssSI C'ACGA_G Cuts at: 3981 5099 6111 3981 Size: 1118 1012 4909 Fragments arranged by size: 4909 1118 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3328 794 Size: 2534 4505 BstBI TT'CG_AA Cuts at: 5843 5843 Size: 7039 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7039 BstXI CCAn_nnnn'nTGG Cuts at: 2188 2518 2907 2997 3015 3033 3051 3069 3123 Size: 330 389 90 18 18 18 18 54 Cuts at: 3123 3141 3159 3177 3231 3249 3285 3318 2188 Size: 18 18 18 54 18 36 33 5909 Fragments arranged by size: 5909 389 330 90 54 54 36 33 18 18 18 18 18 18 18 18 BstYI r'GATC_y Cuts at: 1972 2109 3288 3808 4177 4369 4736 5121 5138 Size: 137 1179 520 369 192 367 385 17 Cuts at: 5138 6590 6676 6688 7034 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1179 520 385 369 367 346 192 137 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2920 2920 Size: 7039 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5548 Fragments arranged by size: 5548 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6300 Fragments arranged by size: 6300 683 56 BtsI GCAGTG_nn' Cuts at: 2794 3236 4555 5349 5369 2794 Size: 442 1319 794 20 4464 Fragments arranged by size: 4464 1319 794 442 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7039 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7004 DraI TTT'AAA Cuts at: 2239 4343 2239 Size: 2104 4935 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2830 6046 809 Size: 507 1514 3216 1802 Fragments arranged by size: 3216 1802 1514 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2520 2619 3035 Size: 54 1425 50 275 18 690 99 416 Cuts at: 3035 3071 3107 3215 3233 3251 3269 3302 4486 Size: 36 36 108 18 18 18 33 1184 Cuts at: 4486 5375 7004 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1184 889 690 416 275 108 99 54 50 43 36 36 33 18 18 18 18 EagI C'GGCC_G Cuts at: 1537 1812 4486 1537 Size: 275 2674 4090 Fragments arranged by size: 4090 2674 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2772 4969 1353 Size: 1419 2197 3423 Fragments arranged by size: 3423 2197 1419 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5626 6000 6146 387 Size: 1064 138 4037 374 146 1280 Fragments arranged by size: 4037 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2738 2848 2958 3174 3401 4089 4138 Size: 393 1106 110 110 216 227 688 49 Cuts at: 4138 4219 5102 5699 6486 6754 1239 Size: 81 883 597 787 268 1524 Fragments arranged by size: 1524 1106 883 787 688 597 393 268 227 216 110 110 81 49 EcoNI CCTnn'n_nnAGG Cuts at: 3416 3752 3416 Size: 336 6703 EcoO109I rG'GnC_Cy Cuts at: 2572 2748 2806 2823 3185 3203 3907 4113 2572 Size: 176 58 17 362 18 704 206 5498 Fragments arranged by size: 5498 704 362 206 176 58 18 17 EcoRI G'AATT_C Cuts at: 4191 4348 4726 4191 Size: 157 378 6504 Fragments arranged by size: 6504 378 157 EcoRV GAT'ATC Cuts at: 3505 3505 Size: 7039 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2251 2353 2251 Size: 102 6937 FspI TGC'GCA Cuts at: 5545 5545 Size: 7039 HaeII r_GCGC'y Cuts at: 1678 2076 3487 6186 1678 Size: 398 1411 2699 2531 Fragments arranged by size: 2699 2531 1411 398 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3492 3524 3783 3815 4091 Size: 32 135 32 1920 32 259 32 276 Cuts at: 4091 4123 4169 4201 5680 5712 5754 5786 1373 Size: 32 46 32 1479 32 42 32 2626 Fragments arranged by size: 2626 1920 1479 276 259 135 46 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4383 4629 669 Size: 988 281 2445 246 3079 Fragments arranged by size: 3079 2445 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7039 HpaI GTT'AAC Cuts at: 4629 4629 Size: 7039 KpnI G_GTAC'C Cuts at: 4364 4364 Size: 7039 MfeI C'AATT_G Cuts at: 4638 4638 Size: 7039 MluI A'CGCG_T Cuts at: 6915 6915 Size: 7039 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6153 6337 1305 Size: 4848 184 2007 Fragments arranged by size: 4848 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2522 2621 3037 3073 3235 Size: 54 1425 343 690 99 416 36 162 Cuts at: 3235 3253 3271 3304 10 Size: 18 18 33 3745 Fragments arranged by size: 3745 1425 690 416 343 162 99 54 36 33 18 18 MslI CAynn'nnrTG Cuts at: 518 1166 3139 3157 3470 3716 4224 4997 5356 Size: 648 1973 18 313 246 508 773 359 Cuts at: 5356 5515 518 Size: 159 2042 Fragments arranged by size: 2042 1973 773 648 508 359 313 246 159 18 MspA1I CmG'CkG Cuts at: 1606 2147 2213 2582 3296 4049 4455 5132 6280 Size: 541 66 369 714 753 406 677 1148 Cuts at: 6280 6525 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 753 714 677 541 406 369 245 66 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5548 Fragments arranged by size: 5548 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7039 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7039 NotI GC'GGCC_GC Cuts at: 4486 4486 Size: 7039 NspI r_CATG'y Cuts at: 1132 1229 3555 4397 5942 1132 Size: 97 2326 842 1545 2229 Fragments arranged by size: 2326 2229 1545 842 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7039 PciI A'CATG_T Cuts at: 5938 5938 Size: 7039 PflMI CCAn_nnn'nTGG Cuts at: 1108 3087 3267 1108 Size: 1979 180 4880 Fragments arranged by size: 4880 1979 180 PfoI T'CCnGG_A Cuts at: 2723 2723 Size: 7039 PmeI GTTT'AAAC Cuts at: 4343 4343 Size: 7039 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3881 3913 5108 5140 6621 6653 3881 Size: 32 1195 32 1481 32 4267 Fragments arranged by size: 4267 1481 1195 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2748 2823 3185 3203 2748 Size: 75 362 18 6584 Fragments arranged by size: 6584 362 75 18 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7039 PsiI TTA'TAA Cuts at: 4609 4609 Size: 7039 PspOMI G'GGCC_C Cuts at: 2470 2805 4112 2470 Size: 335 1307 5397 Fragments arranged by size: 5397 1307 335 PstI C_TGCA'G Cuts at: 830 2821 4015 4048 4391 830 Size: 1991 1194 33 343 3478 Fragments arranged by size: 3478 1991 1194 343 33 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7039 PvuII CAG'CTG Cuts at: 2147 2213 2582 4049 2147 Size: 66 369 1467 5137 Fragments arranged by size: 5137 1467 369 66 SalI G'TCGA_C Cuts at: 1936 4381 1936 Size: 2445 4594 SbfI CC_TGCA'GG Cuts at: 4391 4391 Size: 7039 ScaI AGT'ACT Cuts at: 3446 5287 6999 3446 Size: 1841 1712 3486 Fragments arranged by size: 3486 1841 1712 SfcI C'TryA_G Cuts at: 826 1045 2817 3859 4011 4044 4387 5522 6203 Size: 219 1772 1042 152 33 343 1135 681 Cuts at: 6203 6394 826 Size: 191 1471 Fragments arranged by size: 1772 1471 1135 1042 681 343 219 191 152 33 SfiI GGCCn_nnn'nGGCC Cuts at: 2444 2618 2444 Size: 174 6865 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7039 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7039 SmaI CCC'GGG Cuts at: 1872 4064 4366 1872 Size: 2192 302 4545 Fragments arranged by size: 4545 2192 302 SmlI C'TyrA_G Cuts at: 820 1017 1943 3455 5143 6044 6306 820 Size: 197 926 1512 1688 901 262 1553 Fragments arranged by size: 1688 1553 1512 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4830 493 Size: 4337 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7039 SphI G_CATG'C Cuts at: 1132 3555 4397 1132 Size: 2423 842 3774 Fragments arranged by size: 3774 2423 842 SrfI GCCC'GGGC Cuts at: 4064 4064 Size: 7039 SspI AAT'ATT Cuts at: 5 52 4963 5 Size: 47 4911 2081 Fragments arranged by size: 4911 2081 47 StuI AGG'CCT Cuts at: 1517 2057 2224 2533 4022 1517 Size: 540 167 309 1489 4534 Fragments arranged by size: 4534 1489 540 309 167 StyI C'CwwG_G Cuts at: 513 1065 2004 2304 2328 3802 4023 4478 513 Size: 552 939 300 24 1474 221 455 3074 Fragments arranged by size: 3074 1474 939 552 455 300 221 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2660 3674 3691 3695 5066 5083 5236 Size: 1255 1080 1014 17 4 1371 17 153 Cuts at: 5236 5421 5866 325 Size: 185 445 1498 Fragments arranged by size: 1498 1371 1255 1080 1014 445 185 153 17 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3444 5285 6997 Size: 274 80 33 51 1443 1467 1841 1712 Cuts at: 6997 96 Size: 138 Fragments arranged by size: 1841 1712 1467 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6999 7031 6999 Size: 32 7007 Tth111I GACn'n_nGTC Cuts at: 1316 3777 1316 Size: 2461 4578 XbaI T'CTAG_A Cuts at: 4375 4375 Size: 7039 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2464 4203 2464 Size: 1739 5300 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7039 XmaI C'CCGG_G Cuts at: 1870 4062 4364 1870 Size: 2192 302 4545 Fragments arranged by size: 4545 2192 302 XmnI GAAnn'nnTTC Cuts at: 5168 5168 Size: 7039 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4847 276 Size: 53 83 186 936 3313 2468 Fragments arranged by size: 3313 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SwaI