Restriction map for
pFN21AB5664
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbrPI BbvCI
BciVI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaWI BseAI BseRI
BsiEI BsiWI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstXI BstZ17I CelII ClaI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FseI FspI HindIII HinfI HpaI
KpnI KspI MaeI MfeI MluI
MroI MseI MspA1I MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
SacI SacII SalI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI SspBI SspI
SwaI TfiI Tru9I Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcsI 205 AAATTT [GA]^AATT[CT]
AocI 336 CCTCAGG CC^T[ACGT]AGG
ApaI 965 GGGCCC GGGCC^C
ApoI 205 AAATTT [GA]^AATT[CT]
Asp700I 303 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 965 GGGCCC G[GA]GC[CT]^C
BbeI 505 GGCGCC GGCGC^C
BclI 419 TGATCA T^GATCA
BcnI 882 CCCGG CC^[GC]GG
BglI 361 GCCCTTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 25 CTCCAG CTGGAG(16/14)
BsaAI 70 TACGTG [CT]AC^GT[GA]
BsaBI 183 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 46 GAGACC GGTCTC(1/5)
BsmBI 22 CGTCTC CGTCTC(1/5)
BstEII 624 GGTGACC G^GT[ACGT]ACC
BstPI 624 GGTGACC G^GT[ACGT]ACC
BstYI 1121 GGATCT [GA]^GATC[CT]
Bsu36I 336 CCTCAGG CC^T[ACGT]AGG
CpoI 727 CGGACCG CG^G[AT]CCG
DraII 964 GGGGCCC [GA]G^G[ACGT]CC[CT]
Eco81I 336 CCTCAGG CC^T[ACGT]AGG
EcoO109I 964 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 624 GGTGACC G^GT[ACGT]ACC
FbaI 419 TGATCA T^GATCA
HaeII 505 GGCGCC [GA]GCGC^[CT]
HincII 280 GTCAAC GT[CT]^[GA]AC
HindII 280 GTCAAC GT[CT]^[GA]AC
KasI 505 GGCGCC G^GCGCC
MaeIII 625 GTGAC ^GT[ACGT]AC
MamI 183 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1121 GGATCT [GA]^GATC[CT]
NaeI 262 GCCGGC GCC^GGC
NarI 505 GGCGCC GG^CGCC
NciI 882 CCCGG CC^[GC]GG
NcoI 713 CCATGG C^CATGG
NgoMIV 262 GCCGGC G^CCGGC
NspI 218 GCATGT [GA]CATG^[CT]
RsrII 727 CGGACCG CG^G[AT]CCG
SapI 132 GCTCTTC GCTCTTC(1/4)
SbfI 803 CCTGCAGG CCTGCA^GG
SfoI 505 GGCGCC GGC^GCC
Sse8387I 803 CCTGCAGG CCTGCA^GG
Tsp45I 625 GTGAC ^GT[GC]AC
Tsp509I 206 AATT ^AATT
XhoII 1121 GGATCT [GA]^GATC[CT]
XmnI 303 GAAGACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AluI 151 AGCT AG^CT
382 AGCT
AlwI 636 GATCC GGATC(4/5)
1121 GGATC
AspHI 130 GTGCTC G[AT]GC[AT]^C
862 GTGCTC
BanI 452 GGCACC G^G[CT][GA]CC
505 GGCGCC
BcgI 149 GCAGCTTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
347 GCAGTATGTTCG
BsgI 535 CTGCAC GTGCAG(16/14)
811 GTGCAG
BsiHKAI 130 GTGCTC G[AT]GC[AT]^C
862 GTGCTC
BsmFI 957 GGGAC GGGAC(10/14)
768 GTCCC
BsrBI 403 GAGCGG CCGCTC(-3/-3)
706 GAGCGG
BsrDI 489 CATTGC GCAATG(2/0)
588 CATTGC
BsrFI 30 GCCGGT [GA]^CCGG[CT]
262 GCCGGC
BsrI 387 ACTGG ACTGG(1/-1)
66 CCAGT
Cfr10I 30 GCCGGT [GA]^CCGG[CT]
262 GCCGGC
DdeI 337 CTCAG C^T[ACGT]AG
839 CTCAG
EcoNI 336 CCTCAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
828 CCTCTTCAAGG
EcoT14I 363 CCTTGG C^C[AT][AT]GG
713 CCATGG
MaeII 71 ACGT A^CGT
307 ACGT
PflMI 66 CCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
323 CCACGGTCTGG
StuI 101 AGGCCT AGG^CCT
430 AGGCCT
StyI 363 CCTTGG C^C[AT][AT]GG
713 CCATGG
TaqI 401 TCGA T^CGA
995 TCGA
TspRI 719 CACTG CA[GC]TG[ACGT][ACGT]^
841 CAGTG
TthHB8I 401 TCGA T^CGA
995 TCGA
Van91I 66 CCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
323 CCACGGTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AcyI AfaI AvaII
BalI BbsI BbvI BmyI BpuAI
BsaHI BsaJI BsiYI BslI BsmAI
Bsp1286I BspMI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DpnII
DsaI EaeI EarI EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII HphI
ItaI Ksp632I MboI MboII MluNI
MnlI MscI MslI MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TseI