(Circular) (Six-base) MAPSORT of: pFN21AB5611.seq Check: 6527 from: 1 to: 5851 pFN21AB5611 5851 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2528 2528 Size: 5851 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3661 278 Size: 53 83 186 936 2125 2468 Fragments arranged by size: 2468 2125 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2820 3194 1937 Size: 883 374 4594 Fragments arranged by size: 4594 883 374 Acc65I G'GTAC_C Cuts at: 3172 3172 Size: 5851 AclI AA'CG_TT Cuts at: 1563 3978 4351 1563 Size: 2415 373 3063 Fragments arranged by size: 3063 2415 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2682 2706 3914 5298 5566 1632 Size: 1050 24 1208 1384 268 1917 Fragments arranged by size: 1917 1384 1208 1050 268 24 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5654 AflIII A'CryG_T Cuts at: 1203 4750 5727 1203 Size: 3547 977 1327 Fragments arranged by size: 3547 1327 977 AgeI A'CCGG_T Cuts at: 4682 4682 Size: 5851 AhdI GACnn_n'nnGTC Cuts at: 2780 4580 2780 Size: 1800 4051 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5851 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5851 AlwNI CAG_nnn'CTG Cuts at: 2660 3594 5166 2660 Size: 934 1572 3345 Fragments arranged by size: 3345 1572 934 ApaI G_GGCC'C Cuts at: 2593 2593 Size: 5851 ApaLI G'TGCA_C Cuts at: 3908 5064 3908 Size: 1156 4695 ApoI r'AATT_y Cuts at: 1493 2526 3160 3386 3538 5647 1493 Size: 1033 634 226 152 2109 1697 Fragments arranged by size: 2109 1697 1033 634 226 152 AseI AT'TA_AT Cuts at: 160 1032 4405 160 Size: 872 3373 1606 Fragments arranged by size: 3373 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5851 AvaI C'yCGr_G Cuts at: 1870 1943 3176 1870 Size: 73 1233 4545 Fragments arranged by size: 4545 1233 73 BamHI G'GATC_C Cuts at: 3181 3548 3181 Size: 367 5484 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3172 4627 618 Size: 325 217 627 1385 1455 1842 Fragments arranged by size: 1842 1455 1385 627 325 217 BanII G_rGCy'C Cuts at: 1391 2593 2754 3170 1391 Size: 1202 161 416 4072 Fragments arranged by size: 4072 1202 416 161 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4880 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4042 4076 1903 Size: 34 2105 34 3678 Fragments arranged by size: 3678 2105 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3743 4953 3743 Size: 1210 4641 BclI T'GATC_A Cuts at: 1548 2695 1548 Size: 1147 4704 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4462 136 Size: 107 122 71 631 605 2790 1525 Fragments arranged by size: 2790 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2681 5846 2681 Size: 3165 2686 BlpI GC'TnA_GC Cuts at: 2081 3268 2081 Size: 1187 4664 Bme1580I G_kGCm'C Cuts at: 1790 2593 3912 5068 1790 Size: 803 1319 1156 2573 Fragments arranged by size: 2573 1319 1156 803 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5851 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3582 4540 442 Size: 416 53 83 399 24 2165 958 1753 Fragments arranged by size: 2165 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5851 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2342 2534 4511 1239 Size: 1103 192 1977 2579 Fragments arranged by size: 2579 1977 1103 192 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2428 2816 3976 4841 5139 2428 Size: 388 1160 865 298 3140 Fragments arranged by size: 3140 1160 865 388 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4514 882 Size: 617 3015 2219 Fragments arranged by size: 3015 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3642 493 Size: 711 2438 2702 Fragments arranged by size: 2702 2438 711 BsaBI GATnn'nnATC Cuts at: 2867 5845 2867 Size: 2978 2873 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2982 3658 4040 4675 Size: 53 83 186 936 1449 676 382 635 Cuts at: 4675 275 Size: 1451 Fragments arranged by size: 1451 1449 936 676 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4284 4682 4956 5103 5514 4284 Size: 398 274 147 411 4621 Fragments arranged by size: 4621 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2909 2939 1178 Size: 30 165 30 1506 30 4090 Fragments arranged by size: 4090 1506 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5851 BseYI C'CCAG_C Cuts at: 3032 5054 5550 3032 Size: 2022 496 3333 Fragments arranged by size: 3333 2022 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2388 1163 Size: 573 652 4626 Fragments arranged by size: 4626 652 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3301 4062 5090 1540 Size: 275 186 1300 761 1028 2301 Fragments arranged by size: 2301 1300 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2278 2754 3912 3997 5068 2278 Size: 476 1158 85 1071 3061 Fragments arranged by size: 3061 1158 1071 476 85 BsmI GAATG_Cn' Cuts at: 2539 2948 3362 3455 2539 Size: 409 414 93 4935 Fragments arranged by size: 4935 414 409 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2278 2593 2754 3170 3912 3997 5068 Size: 399 488 315 161 416 742 85 1071 Cuts at: 5068 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 488 416 399 315 161 85 BspEI T'CCGG_A Cuts at: 5514 5514 Size: 5851 BspHI T'CATG_A Cuts at: 3740 5470 3740 Size: 1730 4121 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2528 3206 844 Size: 1684 678 3489 Fragments arranged by size: 3489 1684 678 BsrBI CCG'CTC Cuts at: 1394 1823 3738 1394 Size: 429 1915 3507 Fragments arranged by size: 3507 1915 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2815 4346 4520 65 Size: 1183 1567 1531 174 1396 Fragments arranged by size: 1567 1531 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4495 4682 1700 Size: 110 2685 187 2869 Fragments arranged by size: 2869 2685 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5851 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5851 BssSI C'ACGA_G Cuts at: 3911 4923 3911 Size: 1012 4839 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5851 BstBI TT'CG_AA Cuts at: 4655 4655 Size: 5851 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5851 BstYI r'GATC_y Cuts at: 1972 2681 2768 3181 3548 3933 3950 5402 5488 Size: 709 87 413 367 385 17 1452 86 Cuts at: 5488 5500 5846 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 709 413 385 367 346 87 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2620 513 Size: 552 939 616 3744 Fragments arranged by size: 3744 939 616 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5112 Fragments arranged by size: 5112 683 56 BtsI GCAGTG_nn' Cuts at: 2913 3367 4161 4181 2913 Size: 454 794 20 4583 Fragments arranged by size: 4583 794 454 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5851 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5816 DraI TTT'AAA Cuts at: 2260 3155 2260 Size: 895 4956 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4858 809 Size: 507 3542 1802 Fragments arranged by size: 3542 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3298 4187 5816 Size: 54 1425 50 275 18 1468 889 1629 Cuts at: 5816 8 Size: 43 Fragments arranged by size: 1629 1468 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3298 1537 Size: 275 1486 4090 Fragments arranged by size: 4090 1486 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2742 3781 1353 Size: 1389 1039 3423 Fragments arranged by size: 3423 1389 1039 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4438 4812 4958 387 Size: 1064 138 2849 374 146 1280 Fragments arranged by size: 2849 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2752 2752 Size: 5851 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2342 2534 2682 2706 3914 4511 5298 Size: 393 710 192 148 24 1208 597 787 Cuts at: 5298 5566 1239 Size: 268 1524 Fragments arranged by size: 1524 1208 787 710 597 393 268 192 148 24 EcoO109I rG'GnC_Cy Cuts at: 2589 2590 2630 2589 Size: 1 40 5810 Fragments arranged by size: 5810 40 1 EcoRI G'AATT_C Cuts at: 2526 3160 3538 2526 Size: 634 378 4839 Fragments arranged by size: 4839 634 378 FspI TGC'GCA Cuts at: 3008 4357 3008 Size: 1349 4502 FspAI rTGC'GCAy Cuts at: 3008 3008 Size: 5851 HaeII r_GCGC'y Cuts at: 1678 4998 1678 Size: 3320 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2766 2798 3110 3116 3142 Size: 32 135 32 1194 32 312 6 26 Cuts at: 3142 3148 4492 4524 4566 4598 1373 Size: 6 1344 32 42 32 2626 Fragments arranged by size: 2626 1344 1194 312 135 42 32 32 32 32 32 26 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 2385 2821 3195 3441 669 Size: 988 281 447 436 374 246 3079 Fragments arranged by size: 3079 988 447 436 374 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5851 HpaI GTT'AAC Cuts at: 3441 3441 Size: 5851 KpnI G_GTAC'C Cuts at: 3176 3176 Size: 5851 MfeI C'AATT_G Cuts at: 3450 3450 Size: 5851 MluI A'CGCG_T Cuts at: 5727 5727 Size: 5851 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2907 4965 5149 1305 Size: 1602 2058 184 2007 Fragments arranged by size: 2058 2007 1602 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4029 Fragments arranged by size: 4029 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2858 3809 4168 4327 518 Size: 648 1692 951 359 159 2042 Fragments arranged by size: 2042 1692 951 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2390 2512 3267 3944 5092 5337 1606 Size: 784 122 755 677 1148 245 2120 Fragments arranged by size: 2120 1148 784 755 677 245 122 NcoI C'CATG_G Cuts at: 513 1065 2004 2620 513 Size: 552 939 616 3744 Fragments arranged by size: 3744 939 616 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5851 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5851 NotI GC'GGCC_GC Cuts at: 3298 3298 Size: 5851 NspI r_CATG'y Cuts at: 1132 1229 2092 2192 2718 3209 4754 1132 Size: 97 863 100 526 491 1545 2229 Fragments arranged by size: 2229 1545 863 526 491 100 97 PasI CC'CwG_GG Cuts at: 1348 2050 1348 Size: 702 5149 PciI A'CATG_T Cuts at: 4750 4750 Size: 5851 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5851 PmeI GTTT'AAAC Cuts at: 3155 3155 Size: 5851 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3920 3952 5433 5465 3920 Size: 32 1481 32 4306 Fragments arranged by size: 4306 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2630 2630 Size: 5851 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5851 PsiI TTA'TAA Cuts at: 3421 3421 Size: 5851 PspOMI G'GGCC_C Cuts at: 2589 2589 Size: 5851 PstI C_TGCA'G Cuts at: 830 2272 3203 830 Size: 1442 931 3478 Fragments arranged by size: 3478 1442 931 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5851 PvuII CAG'CTG Cuts at: 2390 2390 Size: 5851 SacI G_AGCT'C Cuts at: 2754 2754 Size: 5851 SalI G'TCGA_C Cuts at: 1936 2819 3193 1936 Size: 883 374 4594 Fragments arranged by size: 4594 883 374 SapI GCTCTTCn'nnn_ Cuts at: 2742 2742 Size: 5851 SbfI CC_TGCA'GG Cuts at: 3203 3203 Size: 5851 ScaI AGT'ACT Cuts at: 4099 5811 4099 Size: 1712 4139 SfcI C'TryA_G Cuts at: 826 1045 2268 2391 3199 4334 5015 5206 826 Size: 219 1223 123 808 1135 681 191 1471 Fragments arranged by size: 1471 1223 1135 808 681 219 191 123 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5851 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5851 SmaI CCC'GGG Cuts at: 1872 3178 1872 Size: 1306 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2443 2795 3955 4856 5118 820 Size: 197 926 500 352 1160 901 262 1553 Fragments arranged by size: 1553 1160 926 901 500 352 262 197 SnaBI TAC'GTA Cuts at: 493 3642 493 Size: 3149 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5851 SphI G_CATG'C Cuts at: 1132 2092 2192 3209 1132 Size: 960 100 1017 3774 Fragments arranged by size: 3774 1017 960 100 SspI AAT'ATT Cuts at: 5 52 3775 5 Size: 47 3723 2081 Fragments arranged by size: 3723 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5851 StyI C'CwwG_G Cuts at: 513 1065 2004 2593 2620 3290 513 Size: 552 939 589 27 670 3074 Fragments arranged by size: 3074 939 670 589 552 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2902 3878 3895 4048 4233 4678 325 Size: 1255 1322 976 17 153 185 445 1498 Fragments arranged by size: 1498 1322 1255 976 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4097 5809 96 Size: 274 80 33 51 1443 2120 1712 138 Fragments arranged by size: 2120 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5811 5843 5811 Size: 32 5819 Tth111I GACn'n_nGTC Cuts at: 1316 2993 1316 Size: 1677 4174 XbaI T'CTAG_A Cuts at: 3187 3187 Size: 5851 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5851 XmaI C'CCGG_G Cuts at: 1870 3176 1870 Size: 1306 4545 XmnI GAAnn'nnTTC Cuts at: 2526 3980 2526 Size: 1454 4397 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3659 276 Size: 53 83 186 936 2125 2468 Fragments arranged by size: 2468 2125 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI