Restriction map for
pFN21AB5603
##################################
# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BanII BbeI BbrPI BbvCI BcgI
BciVI BcnI BfrI BglI BlnI
BlpI BmyI Bpu1102I BsaAI BsaBI
BsaHI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1286I BspDI
BspEI BspHI BsrBI BsrDI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CelII CfoI ClaI
CpoI DraI DraII DraIII DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoT22I FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MslI MspA1I MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I StuI
SwaI Tsp45I Tth111I XbaI XcmI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccII 729 CGCG CG^CG
AlwI 484 GGATC GGATC(4/5)
AocI 181 CCTAAGG CC^T[ACGT]AGG
Asp700I 1339 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 293 CTCGAG C^[CT]CG[GA]G
AvaII 425 GGACC G^G[AT]CC
BanI 1112 GGCACC G^G[CT][GA]CC
BclI 245 TGATCA T^GATCA
BglII 1185 AGATCT A^GATCT
BsaI 621 GAGACC GGTCTC(1/5)
BsaJI 406 CCAAGG C^C[ACGT][ACGT]GG
BsaWI 855 ACCGGA [AT]^CCGG[AT]
BseRI 110 GAGGAG GAGGAG(10/8)
BsoBI 293 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1143 TGTACA T^GTACA
BspMI 825 ACCTGC ACCTGC(4/8)
BsrGI 1143 TGTACA T^GTACA
BstAPI 1377 GCATGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 729 CGCG CG^CG
BstYI 1185 AGATCT [GA]^GATC[CT]
Bsu36I 181 CCTAAGG CC^T[ACGT]AGG
Cfr13I 425 GGACC G^G[ACGT]CC
DrdI 302 GACGGAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 181 CCTAAGG CC^T[ACGT]AGG
EcoNI 103 CCTTCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 406 CCAAGG C^C[AT][AT]GG
FauI 792 CCCGC CCCGC(4/6)
FbaI 245 TGATCA T^GATCA
HaeIII 735 GGCC GG^CC
HincII 252 GTCAAC GT[CT]^[GA]AC
HindII 252 GTCAAC GT[CT]^[GA]AC
MflI 1185 AGATCT [GA]^GATC[CT]
MvnI 729 CGCG CG^CG
PaeR7I 293 CTCGAG C^TCGAG
PflMI 703 CCAGATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 829 GCTCTTC GCTCTTC(1/4)
Sau96I 425 GGACC G^G[ACGT]CC
ScaI 568 AGTACT AGT^ACT
SfaNI 922 GATGC GCATC(5/9)
SfcI 1256 CTATAG C^T[GA][CT]AG
SmlI 293 CTCGAG C^T[CT][GA]AG
SpeI 891 ACTAGT A^CTAGT
SspBI 1143 TGTACA T^GTACA
StyI 406 CCAAGG C^C[AT][AT]GG
Van91I 703 CCAGATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 293 CTCGAG C^TCGAG
XhoII 1185 AGATCT [GA]^GATC[CT]
XmnI 1339 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AciI 793 CCGC CCGC(-3/-1)
730 GCGG
AfaI 569 GTAC GT^AC
1144 GTAC
AflIII 354 ACATGT A^C[GA][CT]GT
786 ACATGT
BsmFI 424 GGGAC GGGAC(10/14)
790 GTCCC
BspLU11I 354 ACATGT A^CATGT
786 ACATGT
BsrFI 136 ACCGGC [GA]^CCGG[CT]
1115 ACCGGC
BstF5I 552 GGATG GGATG(2/0)
882 GGATG
Cac8I 98 GCCTGC GC[ACGT]^[ACGT]GC
1373 GCTTGC
Cfr10I 136 ACCGGC [GA]^CCGG[CT]
1115 ACCGGC
EcoRI 859 GAATTC G^AATTC
1000 GAATTC
EcoRV 288 GATATC GAT^ATC
673 GATATC
FokI 552 GGATG GGATG(9/13)
882 GGATG
HindIII 20 AAGCTT A^AGCTT
1321 AAGCTT
MaeIII 697 GTAAC ^GT[ACGT]AC
1074 GTTAC
MwoI 505 GCTGTGGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1377 GCATGATTTGC
NlaIV 424 GGGACC GG[ACGT]^[ACGT]CC
1112 GGCACC
NspI 354 ACATGT [GA]CATG^[CT]
786 ACATGT
RsaI 569 GTAC GT^AC
1144 GTAC
SspI 77 AATATT AAT^ATT
1171 AATATT
TspRI 57 CACTG CA[GC]TG[ACGT][ACGT]^
797 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AcsI AluI ApoI BbsI BbvI
BfaI BpmI BpuAI BsiYI BslI
BsmAI BsrI BssKI BstNI DdeI
DpnII EarI EcoRII Fnu4HI HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MboI MboII MnlI
MseI MspI MvaI NdeII NlaIII
Sau3AI ScrFI TaqI TfiI Tru9I
TseI Tsp509I TthHB8I