Restriction map for
pFN21AB5596
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AflII AgeI
Alw44I AlwNI AocI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspHI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BcgI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaI BsgI BsiHKAI BsiWI BsmBI
BsoBI BspDI BspLU11I BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI Eco81I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI HphI KasI
KpnI MflI MluI NaeI NarI
NcoI NgoMIV NheI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tth111I Van91I XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccII 147 CGCG CG^CG
AccIII 1155 TCCGGA T^CCGGA
AclI 606 AACGTT AA^CGTT
AcyI 720 GACGCC G[GA]^CG[CT]C
AflIII 1837 ACACGT A^C[GA][CT]GT
AhdI 1386 GACTGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1376 AGCGCT AGC^GCT
AspEI 1386 GACTGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 944 GGTGCC G^G[CT][GA]CC
BbvCI 263 CCTCAGC CCTCAGC(-5/-2)
BciVI 1158 GGATAC GTATCC(6/5)
BglI 7 GCCAATGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 720 GACGCC G[GA]^CG[CT]C
BsaWI 1155 TCCGGA [AT]^CCGG[AT]
BseAI 1155 TCCGGA T^CCGGA
BsiEI 251 CGGCCG CG[GA][CT]^CG
Bsp1407I 1293 TGTACA T^GTACA
BspEI 1155 TCCGGA T^CCGGA
BspHI 1332 TCATGA T^CATGA
BspMI 1166 ACCTGC ACCTGC(4/8)
BsrDI 1464 GCAATG GCAATG(2/0)
BsrGI 1293 TGTACA T^GTACA
BstUI 147 CGCG CG^CG
BstXI 162 CCACGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 251 CGGCCG C^GGCCG
Eam1105I 1386 GACTGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 251 CGGCCG C^GGCCG
Eco47III 1376 AGCGCT AGC^GCT
Eco52I 251 CGGCCG C^GGCCG
EcoNI 912 CCTGCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1523 GAATTC G^AATTC
EcoT14I 311 CCTTGG C^C[AT][AT]GG
HgaI 720 GACGC GACGC(5/10)
Hin1I 720 GACGCC G[GA]^CG[CT]C
KspI 146 CCGCGG CCGC^GG
MfeI 1814 CAATTG C^AATTG
MroI 1155 TCCGGA T^CCGGA
MunI 1814 CAATTG C^AATTG
MvnI 147 CGCG CG^CG
NdeI 1140 CATATG CA^TATG
NotI 250 GCGGCCGC GC^GGCCGC
NspI 1197 ACATGC [GA]CATG^[CT]
Psp1406I 606 AACGTT AA^CGTT
PstI 646 CTGCAG CTGCA^G
PvuII 278 CAGCTG CAG^CTG
RcaI 1332 TCATGA T^CATGA
SacII 146 CCGCGG CCGC^GG
SfcI 646 CTGCAG C^T[GA][CT]AG
SspBI 1293 TGTACA T^GTACA
SspI 1951 AATATT AAT^ATT
StyI 311 CCTTGG C^C[AT][AT]GG
TaqI 1541 TCGA T^CGA
TthHB8I 1541 TCGA T^CGA
XbaI 978 TCTAGA T^CTAGA
XcmI 317 CCACCCCAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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BalI 314 TGGCCA TGG^CCA
1886 TGGCCA
BanII 172 GGGCTC G[GA]GC[CT]^C
415 GGGCTC
BcnI 87 CCCGG CC^[GC]GG
241 CCCGG
BsaAI 794 TACGTG [CT]AC^GT[GA]
1838 CACGTA
BsaBI 970 GATAATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1426 GATGGTAATC
DsaI 146 CCGCGG C^C[GA][CT]GG
892 CCACGG
EarI 306 CTCTTC CTCTTC(1/4)
1957 GAAGAG
HaeII 1376 AGCGCT [GA]GCGC^[CT]
1891 AGCGCC
Ksp632I 306 CTCTTC CTCTTC(1/4)
1957 GAAGAG
MamI 970 GATAATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1426 GATGGTAATC
MluNI 314 TGGCCA TGG^CCA
1886 TGGCCA
MscI 314 TGGCCA TGG^CCA
1886 TGGCCA
MseI 1181 TTAA T^TAA
1627 TTAA
NciI 87 CCCGG CC^[GC]GG
241 CCCGG
SmlI 340 CTCAAG C^T[CT][GA]AG
1479 CTTGAG
Tru9I 1181 TTAA T^TAA
1627 TTAA
Tsp45I 1384 GTGAC ^GT[GC]AC
1418 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
TfiI TseI Tsp509I TspRI