(Circular) (Six-base) MAPSORT of: pFN21AB5596.seq Check: 23 from: 1 to: 6688 pFN21AB5596 6688 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3180 3180 Size: 6688 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4498 278 Size: 53 83 186 936 2962 2468 Fragments arranged by size: 2962 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4031 1937 Size: 2094 4594 Acc65I G'GTAC_C Cuts at: 4009 4009 Size: 6688 AclI AA'CG_TT Cuts at: 1563 2612 4815 5188 1563 Size: 1049 2203 373 3063 Fragments arranged by size: 3063 2203 1049 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2846 4751 6135 6403 1632 Size: 1214 1905 1384 268 1917 Fragments arranged by size: 1917 1905 1384 1214 268 AfeI AGC'GCT Cuts at: 3383 3383 Size: 6688 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6491 AflIII A'CryG_T Cuts at: 1203 3842 5587 6564 1203 Size: 2639 1745 977 1327 Fragments arranged by size: 2639 1745 1327 977 AgeI A'CCGG_T Cuts at: 5519 5519 Size: 6688 AhdI GACnn_n'nnGTC Cuts at: 3396 5417 3396 Size: 2021 4667 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6688 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6688 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3486 3518 3486 Size: 32 6656 AlwNI CAG_nnn'CTG Cuts at: 4431 6003 4431 Size: 1572 5116 ApaLI G'TGCA_C Cuts at: 4745 5901 4745 Size: 1156 5532 ApoI r'AATT_y Cuts at: 1493 2489 3089 3528 3997 4223 4375 6484 1493 Size: 996 600 439 469 226 152 2109 1697 Fragments arranged by size: 2109 1697 996 600 469 439 226 152 AseI AT'TA_AT Cuts at: 160 1032 5242 160 Size: 872 4210 1606 Fragments arranged by size: 4210 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6688 AvaI C'yCGr_G Cuts at: 1870 1943 4013 1870 Size: 73 2070 4545 Fragments arranged by size: 4545 2070 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3123 3156 3123 Size: 33 6655 BamHI G'GATC_C Cuts at: 4018 4385 4018 Size: 367 6321 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2949 4009 5464 618 Size: 325 217 627 1162 1060 1455 1842 Fragments arranged by size: 1842 1455 1162 1060 627 325 217 BanII G_rGCy'C Cuts at: 1391 2181 2424 4007 1391 Size: 790 243 1583 4072 Fragments arranged by size: 4072 1583 790 243 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2799 3348 3759 928 Size: 971 900 549 411 3857 Fragments arranged by size: 3857 971 900 549 411 BbvCI CC'TCA_GC Cuts at: 2269 2269 Size: 6688 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4879 4913 1903 Size: 34 50 34 2858 34 3678 Fragments arranged by size: 3678 2858 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3157 4580 5790 3157 Size: 1423 1210 4055 Fragments arranged by size: 4055 1423 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6688 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2018 5299 Size: 107 122 71 631 605 337 9 3281 Cuts at: 5299 136 Size: 1525 Fragments arranged by size: 3281 1525 631 605 337 122 107 71 9 BglII A'GATC_T Cuts at: 6683 6683 Size: 6688 BlpI GC'TnA_GC Cuts at: 4105 4105 Size: 6688 Bme1580I G_kGCm'C Cuts at: 1790 3840 4749 5905 1790 Size: 2050 909 1156 2573 Fragments arranged by size: 2573 2050 1156 909 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6688 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2342 2362 2527 Size: 416 53 83 399 24 925 20 165 Cuts at: 2527 4419 5377 442 Size: 1892 958 1753 Fragments arranged by size: 1892 1753 958 925 416 399 165 83 53 24 20 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6688 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3351 3351 Size: 6688 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2696 2714 3414 5348 1239 Size: 1457 18 700 1934 2579 Fragments arranged by size: 2579 1934 1457 700 18 Bpu10I CC'TnA_GC Cuts at: 2269 2416 2269 Size: 147 6541 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2330 3505 4813 5678 5976 2330 Size: 1175 1308 865 298 3042 Fragments arranged by size: 3042 1308 1175 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5351 882 Size: 617 3852 2219 Fragments arranged by size: 3852 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2801 3845 4479 493 Size: 711 1597 1044 634 2702 Fragments arranged by size: 2702 1597 1044 711 634 BsaBI GATnn'nnATC Cuts at: 2979 3435 6682 2979 Size: 456 3247 2985 Fragments arranged by size: 3247 2985 456 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2726 4495 4877 5512 Size: 53 83 186 936 1193 1769 382 635 Cuts at: 5512 275 Size: 1451 Fragments arranged by size: 1769 1451 1193 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3160 5121 5519 5793 5940 6351 3160 Size: 1961 398 274 147 411 3497 Fragments arranged by size: 3497 1961 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2898 2928 1178 Size: 30 165 30 1495 30 4938 Fragments arranged by size: 4938 1495 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2040 2043 2046 2049 2073 2076 2299 2432 Size: 854 3 3 3 24 3 223 133 Cuts at: 2432 2559 1186 Size: 127 5315 Fragments arranged by size: 5315 854 223 133 127 24 3 3 3 3 BseYI C'CCAG_C Cuts at: 5891 6387 5891 Size: 496 6192 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6115 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2259 4138 4899 5927 1540 Size: 275 186 258 1879 761 1028 2301 Fragments arranged by size: 2301 1879 1028 761 275 258 186 BsiHKAI G_wGCw'C Cuts at: 4749 4834 5905 4749 Size: 85 1071 5532 Fragments arranged by size: 5532 1071 85 BsmI GAATG_Cn' Cuts at: 2563 2751 3974 4199 4292 2563 Size: 188 1223 225 93 4959 Fragments arranged by size: 4959 1223 225 188 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2181 2424 3840 4007 4749 4834 5905 Size: 399 391 243 1416 167 742 85 1071 Cuts at: 5905 1391 Size: 2174 Fragments arranged by size: 2174 1416 1071 742 399 391 243 167 85 BspEI T'CCGG_A Cuts at: 3160 6351 3160 Size: 3191 3497 BspHI T'CATG_A Cuts at: 3337 4577 6307 3337 Size: 1240 1730 3718 Fragments arranged by size: 3718 1730 1240 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3180 4043 844 Size: 2336 863 3489 Fragments arranged by size: 3489 2336 863 BsrBI CCG'CTC Cuts at: 1394 1823 4575 1394 Size: 429 2752 3507 Fragments arranged by size: 3507 2752 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3476 5183 5357 65 Size: 1183 2228 1707 174 1396 Fragments arranged by size: 2228 1707 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5332 5519 1700 Size: 110 3522 187 2869 Fragments arranged by size: 3522 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3298 96 Size: 3202 3486 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6688 BssSI C'ACGA_G Cuts at: 4748 5760 4748 Size: 1012 5676 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6688 BstBI TT'CG_AA Cuts at: 5492 5492 Size: 6688 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6688 BstXI CCAn_nnnn'nTGG Cuts at: 2174 2174 Size: 6688 BstYI r'GATC_y Cuts at: 1972 4018 4385 4770 4787 6239 6325 6337 6683 Size: 2046 367 385 17 1452 86 12 346 Cuts at: 6683 1972 Size: 1977 Fragments arranged by size: 2046 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2151 2897 513 Size: 552 1086 746 4304 Fragments arranged by size: 4304 1086 746 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3745 487 Size: 683 56 2519 3430 Fragments arranged by size: 3430 2519 683 56 BtsI GCAGTG_nn' Cuts at: 3778 4204 4998 5018 3778 Size: 426 794 20 5448 Fragments arranged by size: 5448 794 426 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6688 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6653 DraI TTT'AAA Cuts at: 3992 3992 Size: 6688 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5695 809 Size: 507 4379 1802 Fragments arranged by size: 4379 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2256 2319 3616 Size: 54 1425 50 275 18 426 63 1297 Cuts at: 3616 3891 4135 5024 6653 8 Size: 275 244 889 1629 43 Fragments arranged by size: 1629 1425 1297 889 426 275 275 244 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2256 4135 1537 Size: 275 444 1879 4090 Fragments arranged by size: 4090 1879 444 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2317 3957 4618 1353 Size: 964 1640 661 3423 Fragments arranged by size: 3423 1640 964 661 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2179 2188 2281 5275 5649 5795 Size: 1064 138 590 9 93 2994 374 146 Cuts at: 5795 387 Size: 1280 Fragments arranged by size: 2994 1280 1064 590 374 146 138 93 9 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2696 2714 2846 3414 4751 5348 6135 Size: 393 1064 18 132 568 1337 597 787 Cuts at: 6135 6403 1239 Size: 268 1524 Fragments arranged by size: 1524 1337 1064 787 597 568 393 268 132 18 EcoNI CCTnn'n_nnAGG Cuts at: 2921 2921 Size: 6688 EcoO109I rG'GnC_Cy Cuts at: 2158 2689 3550 2158 Size: 531 861 5296 Fragments arranged by size: 5296 861 531 EcoRI G'AATT_C Cuts at: 3528 3997 4375 3528 Size: 469 378 5841 Fragments arranged by size: 5841 469 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3378 3378 Size: 6688 FspI TGC'GCA Cuts at: 5194 5194 Size: 6688 HaeII r_GCGC'y Cuts at: 1678 3385 3900 5835 1678 Size: 1707 515 1935 2531 Fragments arranged by size: 2531 1935 1707 515 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2422 2454 2978 3010 3260 Size: 32 135 32 850 32 524 32 250 Cuts at: 3260 3292 3382 3414 5329 5361 5403 5435 1373 Size: 32 90 32 1915 32 42 32 2626 Fragments arranged by size: 2626 1915 850 524 250 135 90 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4032 4278 669 Size: 988 281 2094 246 3079 Fragments arranged by size: 3079 2094 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6688 HpaI GTT'AAC Cuts at: 4278 4278 Size: 6688 KpnI G_GTAC'C Cuts at: 4013 4013 Size: 6688 MfeI C'AATT_G Cuts at: 3819 4287 3819 Size: 468 6220 MluI A'CGCG_T Cuts at: 6564 6564 Size: 6688 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5802 5986 1305 Size: 4497 184 2007 Fragments arranged by size: 4497 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2321 3893 10 Size: 54 1425 343 489 1572 2805 Fragments arranged by size: 2805 1572 1425 489 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2172 2409 3069 3207 4646 5005 5164 Size: 648 1006 237 660 138 1439 359 159 Cuts at: 5164 518 Size: 2042 Fragments arranged by size: 2042 1439 1006 660 648 359 237 159 138 MspA1I CmG'CkG Cuts at: 1606 2153 2285 2731 4104 4781 5929 6174 1606 Size: 547 132 446 1373 677 1148 245 2120 Fragments arranged by size: 2120 1373 1148 677 547 446 245 132 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6136 NdeI CA'TA_TG Cuts at: 387 3146 387 Size: 2759 3929 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6688 NotI GC'GGCC_GC Cuts at: 2256 4135 2256 Size: 1879 4809 NspI r_CATG'y Cuts at: 1132 1229 3206 4046 5591 1132 Size: 97 1977 840 1545 2229 Fragments arranged by size: 2229 1977 1545 840 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6688 PciI A'CATG_T Cuts at: 5587 5587 Size: 6688 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6688 PfoI T'CCnGG_A Cuts at: 2691 2691 Size: 6688 PmeI GTTT'AAAC Cuts at: 3992 3992 Size: 6688 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4757 4789 6270 6302 4757 Size: 32 1481 32 5143 Fragments arranged by size: 5143 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2158 2689 3550 2158 Size: 531 861 5296 Fragments arranged by size: 5296 861 531 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6688 PsiI TTA'TAA Cuts at: 4258 4258 Size: 6688 PstI C_TGCA'G Cuts at: 830 2655 4040 830 Size: 1825 1385 3478 Fragments arranged by size: 3478 1825 1385 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6688 PvuII CAG'CTG Cuts at: 2285 2285 Size: 6688 SacII CC_GC'GG Cuts at: 2154 2154 Size: 6688 SalI G'TCGA_C Cuts at: 1936 4030 1936 Size: 2094 4594 SbfI CC_TGCA'GG Cuts at: 4040 4040 Size: 6688 ScaI AGT'ACT Cuts at: 4936 6648 4936 Size: 1712 4976 SfcI C'TryA_G Cuts at: 826 1045 2651 4036 5171 5852 6043 826 Size: 219 1606 1385 1135 681 191 1471 Fragments arranged by size: 1606 1471 1385 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6688 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6688 SmaI CCC'GGG Cuts at: 1872 4015 1872 Size: 2143 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2345 3484 4792 5693 5955 820 Size: 197 926 402 1139 1308 901 262 1553 Fragments arranged by size: 1553 1308 1139 926 901 402 262 197 SnaBI TAC'GTA Cuts at: 493 4479 493 Size: 3986 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6688 SphI G_CATG'C Cuts at: 1132 4046 1132 Size: 2914 3774 SspI AAT'ATT Cuts at: 5 52 3958 4612 5 Size: 47 3906 654 2081 Fragments arranged by size: 3906 2081 654 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6688 StyI C'CwwG_G Cuts at: 513 1065 2316 4127 513 Size: 552 1251 1811 3074 Fragments arranged by size: 3074 1811 1251 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4715 4732 4885 5070 5515 325 Size: 1255 3135 17 153 185 445 1498 Fragments arranged by size: 3135 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3298 3571 4934 Size: 274 80 33 51 1443 1321 273 1363 Cuts at: 4934 6646 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1363 1321 274 273 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2613 2645 6648 6680 2613 Size: 32 4003 32 2621 Fragments arranged by size: 4003 2621 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6688 XbaI T'CTAG_A Cuts at: 2983 4024 2983 Size: 1041 5647 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2329 2329 Size: 6688 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6688 XmaI C'CCGG_G Cuts at: 1870 4013 1870 Size: 2143 4545 XmnI GAAnn'nnTTC Cuts at: 4817 4817 Size: 6688 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4496 276 Size: 53 83 186 936 2962 2468 Fragments arranged by size: 2962 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI