(Circular) (Six-base) MAPSORT of: pFN21AB5542.seq Check: 6476 from: 1 to: 5779 pFN21AB5542 5779 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3589 278 Size: 53 83 186 936 2053 2468 Fragments arranged by size: 2468 2053 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3122 1937 Size: 1185 4594 Acc65I G'GTAC_C Cuts at: 3100 3100 Size: 5779 AclI AA'CG_TT Cuts at: 1563 3906 4279 1563 Size: 2343 373 3063 Fragments arranged by size: 3063 2343 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3842 5226 5494 1632 Size: 2210 1384 268 1917 Fragments arranged by size: 2210 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5582 AflIII A'CryG_T Cuts at: 1203 2629 2838 4678 5655 1203 Size: 1426 209 1840 977 1327 Fragments arranged by size: 1840 1426 1327 977 209 AgeI A'CCGG_T Cuts at: 4610 4610 Size: 5779 AhdI GACnn_n'nnGTC Cuts at: 4508 4508 Size: 5779 AleI CACnn'nnGTG Cuts at: 1166 2099 2558 1166 Size: 933 459 4387 Fragments arranged by size: 4387 933 459 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5779 AlwNI CAG_nnn'CTG Cuts at: 3522 5094 3522 Size: 1572 4207 ApaI G_GGCC'C Cuts at: 2382 2683 2382 Size: 301 5478 ApaLI G'TGCA_C Cuts at: 3836 4992 3836 Size: 1156 4623 ApoI r'AATT_y Cuts at: 1493 3088 3314 3466 5575 1493 Size: 1595 226 152 2109 1697 Fragments arranged by size: 2109 1697 1595 226 152 AseI AT'TA_AT Cuts at: 160 1032 4333 160 Size: 872 3301 1606 Fragments arranged by size: 3301 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5779 AvaI C'yCGr_G Cuts at: 1870 1943 2867 3104 1870 Size: 73 924 237 4545 Fragments arranged by size: 4545 924 237 73 BamHI G'GATC_C Cuts at: 3109 3476 3109 Size: 367 5412 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2203 2212 2812 3100 4555 Size: 325 217 627 416 9 600 288 1455 Cuts at: 4555 618 Size: 1842 Fragments arranged by size: 1842 1455 627 600 416 325 288 217 9 BanII G_rGCy'C Cuts at: 1391 2072 2127 2382 2619 2683 2757 2874 3005 Size: 681 55 255 237 64 74 117 131 Cuts at: 3005 3098 1391 Size: 93 4072 Fragments arranged by size: 4072 681 255 237 131 117 93 74 64 55 BbeI G_GCGC'C Cuts at: 2216 2216 Size: 5779 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2556 928 Size: 971 657 4151 Fragments arranged by size: 4151 971 657 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2185 2219 3970 4004 1903 Size: 34 248 34 1751 34 3678 Fragments arranged by size: 3678 1751 248 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3671 4881 3671 Size: 1210 4569 BclI T'GATC_A Cuts at: 1548 1548 Size: 5779 BfrBI ATG'CAT Cuts at: 2362 2362 Size: 5779 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2386 2522 4390 Size: 107 122 71 631 605 714 136 1868 Cuts at: 4390 136 Size: 1525 Fragments arranged by size: 1868 1525 714 631 605 136 122 107 71 BglII A'GATC_T Cuts at: 2490 5774 2490 Size: 3284 2495 BlpI GC'TnA_GC Cuts at: 3196 3196 Size: 5779 Bme1580I G_kGCm'C Cuts at: 1790 2382 2683 3055 3840 4996 1790 Size: 592 301 372 785 1156 2573 Fragments arranged by size: 2573 1156 785 592 372 301 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5779 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2155 3510 4468 Size: 416 53 83 399 24 738 1355 958 Cuts at: 4468 442 Size: 1753 Fragments arranged by size: 1753 1355 958 738 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5779 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2603 2633 2841 2985 4439 1239 Size: 1364 30 208 144 1454 2579 Fragments arranged by size: 2579 1454 1364 208 144 30 Bpu10I CC'TnA_GC Cuts at: 3004 3004 Size: 5779 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3904 4769 5067 3904 Size: 865 298 4616 Fragments arranged by size: 4616 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2346 2756 2965 4442 882 Size: 617 847 410 209 1477 2219 Fragments arranged by size: 2219 1477 847 617 410 209 BsaAI yAC'GTr Cuts at: 493 1204 2149 2632 2841 3570 493 Size: 711 945 483 209 729 2702 Fragments arranged by size: 2702 945 729 711 483 209 BsaBI GATnn'nnATC Cuts at: 5773 5773 Size: 5779 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2184 2213 3586 3968 Size: 53 83 186 936 651 29 1373 382 Cuts at: 3968 4603 275 Size: 635 1451 Fragments arranged by size: 1451 1373 936 651 635 382 186 83 53 29 BsaWI w'CCGG_w Cuts at: 4212 4610 4884 5031 5442 4212 Size: 398 274 147 411 4549 Fragments arranged by size: 4549 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2444 2474 2958 2988 3034 Size: 30 165 30 1041 30 484 30 46 Cuts at: 3034 3064 1178 Size: 30 3893 Fragments arranged by size: 3893 1041 484 165 46 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2021 2840 1186 Size: 835 819 4125 Fragments arranged by size: 4125 835 819 BseYI C'CCAG_C Cuts at: 2374 2676 2901 4982 5478 2374 Size: 302 225 2081 496 2675 Fragments arranged by size: 2675 2081 496 302 225 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2870 1163 Size: 573 1134 4072 Fragments arranged by size: 4072 1134 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2116 3229 3990 5018 1540 Size: 275 186 115 1113 761 1028 2301 Fragments arranged by size: 2301 1113 1028 761 275 186 115 BsiHKAI G_wGCw'C Cuts at: 2532 2757 3005 3840 3925 4996 2532 Size: 225 248 835 85 1071 3315 Fragments arranged by size: 3315 1071 835 248 225 85 BsmI GAATG_Cn' Cuts at: 3290 3383 3290 Size: 93 5686 BsmBI CGTCTCn'nnnn_ Cuts at: 2591 2591 Size: 5779 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 2127 2382 2532 2619 2683 2757 Size: 399 282 55 255 150 87 64 74 Cuts at: 2757 2874 3005 3055 3098 3840 3925 4996 1391 Size: 117 131 50 43 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 282 255 150 131 117 87 85 74 64 55 50 43 BspEI T'CCGG_A Cuts at: 5442 5442 Size: 5779 BspHI T'CATG_A Cuts at: 3668 5398 3668 Size: 1730 4049 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2307 2463 3134 844 Size: 1463 156 671 3489 Fragments arranged by size: 3489 1463 671 156 BsrBI CCG'CTC Cuts at: 1394 1823 3666 1394 Size: 429 1843 3507 Fragments arranged by size: 3507 1843 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2928 4274 4448 65 Size: 1183 1680 1346 174 1396 Fragments arranged by size: 1680 1396 1346 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2893 4423 4610 1700 Size: 110 1083 1530 187 2869 Fragments arranged by size: 2869 1530 1083 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5779 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5779 BssSI C'ACGA_G Cuts at: 2756 3839 4851 2756 Size: 1083 1012 3684 Fragments arranged by size: 3684 1083 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2359 794 Size: 1565 4214 BstBI TT'CG_AA Cuts at: 4583 4583 Size: 5779 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5779 BstYI r'GATC_y Cuts at: 1972 2490 2951 3109 3476 3861 3878 5330 5416 Size: 518 461 158 367 385 17 1452 86 Cuts at: 5416 5428 5774 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 518 461 385 367 346 158 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2669 2669 Size: 5779 BtgI C'CryG_G Cuts at: 513 1065 2004 2116 2207 2216 2398 2469 513 Size: 552 939 112 91 9 182 71 3823 Fragments arranged by size: 3823 939 552 182 112 91 71 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5040 Fragments arranged by size: 5040 683 56 BtsI GCAGTG_nn' Cuts at: 2360 3295 4089 4109 2360 Size: 935 794 20 4030 Fragments arranged by size: 4030 935 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5779 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5744 DraI TTT'AAA Cuts at: 3083 3083 Size: 5779 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4786 809 Size: 507 3470 1802 Fragments arranged by size: 3470 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2113 3226 4115 Size: 54 1425 50 275 18 283 1113 889 Cuts at: 4115 5744 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1113 889 283 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2113 3226 1537 Size: 275 301 1113 4090 Fragments arranged by size: 4090 1113 301 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2993 3709 1353 Size: 1640 716 3423 Fragments arranged by size: 3423 1640 716 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2540 4366 4740 4886 387 Size: 1064 138 951 1826 374 146 1280 Fragments arranged by size: 1826 1280 1064 951 374 146 138 EcoICRI GAG'CTC Cuts at: 2755 3003 2755 Size: 248 5531 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2603 2633 2841 2985 3842 4439 5226 Size: 393 971 30 208 144 857 597 787 Cuts at: 5226 5494 1239 Size: 268 1524 Fragments arranged by size: 1524 971 857 787 597 393 268 208 144 30 EcoNI CCTnn'n_nnAGG Cuts at: 2036 2447 2585 2036 Size: 411 138 5230 Fragments arranged by size: 5230 411 138 EcoO109I rG'GnC_Cy Cuts at: 2041 2378 2452 2680 2041 Size: 337 74 228 5140 Fragments arranged by size: 5140 337 228 74 EcoRI G'AATT_C Cuts at: 3088 3466 3088 Size: 378 5401 FspI TGC'GCA Cuts at: 4285 4285 Size: 5779 HaeII r_GCGC'y Cuts at: 1678 2216 2268 2392 4926 1678 Size: 538 52 124 2534 2531 Fragments arranged by size: 2534 2531 538 124 52 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2324 2356 2444 2476 2489 Size: 32 135 32 752 32 88 32 13 Cuts at: 2489 2521 2943 2975 4420 4452 4494 4526 1373 Size: 32 422 32 1445 32 42 32 2626 Fragments arranged by size: 2626 1445 752 422 135 88 42 32 32 32 32 32 32 32 32 13 HincII GTy'rAC Cuts at: 669 1657 1938 3123 3369 669 Size: 988 281 1185 246 3079 Fragments arranged by size: 3079 1185 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5779 HpaI GTT'AAC Cuts at: 3369 3369 Size: 5779 KasI G'GCGC_C Cuts at: 2212 2212 Size: 5779 KpnI G_GTAC'C Cuts at: 3104 3104 Size: 5779 MfeI C'AATT_G Cuts at: 3378 3378 Size: 5779 MluI A'CGCG_T Cuts at: 5655 5655 Size: 5779 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4893 5077 1305 Size: 3588 184 2007 Fragments arranged by size: 3588 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3957 Fragments arranged by size: 3957 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2099 2138 2558 3737 4096 4255 518 Size: 648 933 39 420 1179 359 159 2042 Fragments arranged by size: 2042 1179 933 648 420 359 159 39 MspA1I CmG'CkG Cuts at: 1606 2118 2209 2400 2471 2804 3195 3872 5020 Size: 512 91 191 71 333 391 677 1148 Cuts at: 5020 5265 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 512 391 333 245 191 91 71 NaeI GCC'GGC Cuts at: 2895 2895 Size: 5779 NarI GG'CG_CC Cuts at: 2213 2213 Size: 5779 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4288 Fragments arranged by size: 4288 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5779 NgoMIV G'CCGG_C Cuts at: 2893 2893 Size: 5779 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5779 NotI GC'GGCC_GC Cuts at: 3226 3226 Size: 5779 NsiI A_TGCA'T Cuts at: 2364 2364 Size: 5779 NspI r_CATG'y Cuts at: 1132 1229 2559 3137 4682 1132 Size: 97 1330 578 1545 2229 Fragments arranged by size: 2229 1545 1330 578 97 PasI CC'CwG_GG Cuts at: 1348 2683 3055 1348 Size: 1335 372 4072 Fragments arranged by size: 4072 1335 372 PciI A'CATG_T Cuts at: 4678 4678 Size: 5779 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5779 PfoI T'CCnGG_A Cuts at: 2462 2462 Size: 5779 PmeI GTTT'AAAC Cuts at: 3083 3083 Size: 5779 PmlI CAC'GTG Cuts at: 2632 2841 2632 Size: 209 5570 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3848 3880 5361 5393 3848 Size: 32 1481 32 4234 Fragments arranged by size: 4234 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2452 2452 Size: 5779 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5779 PsiI TTA'TAA Cuts at: 3349 3349 Size: 5779 PspOMI G'GGCC_C Cuts at: 2378 2679 2378 Size: 301 5478 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2396 2428 2396 Size: 32 5747 PstI C_TGCA'G Cuts at: 830 2355 2776 3131 830 Size: 1525 421 355 3478 Fragments arranged by size: 3478 1525 421 355 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5779 PvuII CAG'CTG Cuts at: 2804 2804 Size: 5779 SacI G_AGCT'C Cuts at: 2757 3005 2757 Size: 248 5531 SacII CC_GC'GG Cuts at: 2119 2210 2401 2472 2119 Size: 91 191 71 5426 Fragments arranged by size: 5426 191 91 71 SalI G'TCGA_C Cuts at: 1936 3121 1936 Size: 1185 4594 SapI GCTCTTCn'nnn_ Cuts at: 2993 2993 Size: 5779 SbfI CC_TGCA'GG Cuts at: 2776 3131 2776 Size: 355 5424 ScaI AGT'ACT Cuts at: 4027 5739 4027 Size: 1712 4067 SfcI C'TryA_G Cuts at: 826 1045 2351 2772 3127 4262 4943 5134 826 Size: 219 1306 421 355 1135 681 191 1471 Fragments arranged by size: 1471 1306 1135 681 421 355 219 191 SfoI GGC'GCC Cuts at: 2214 2214 Size: 5779 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5779 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5779 SmaI CCC'GGG Cuts at: 1872 3106 1872 Size: 1234 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3883 4784 5046 820 Size: 197 926 1940 901 262 1553 Fragments arranged by size: 1940 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3570 493 Size: 3077 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5779 SphI G_CATG'C Cuts at: 1132 3137 1132 Size: 2005 3774 SspI AAT'ATT Cuts at: 5 52 3703 5 Size: 47 3651 2081 Fragments arranged by size: 3651 2081 47 StuI AGG'CCT Cuts at: 1517 2770 1517 Size: 1253 4526 StyI C'CwwG_G Cuts at: 513 1065 2004 2947 3218 513 Size: 552 939 943 271 3074 Fragments arranged by size: 3074 943 939 552 271 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2174 3806 3823 3976 4161 4606 325 Size: 1255 594 1632 17 153 185 445 1498 Fragments arranged by size: 1632 1498 1255 594 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4025 5737 96 Size: 274 80 33 51 1443 2048 1712 138 Fragments arranged by size: 2048 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2271 2303 3034 3066 5739 5771 2271 Size: 32 731 32 2673 32 2279 Fragments arranged by size: 2673 2279 731 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5779 XbaI T'CTAG_A Cuts at: 3115 3115 Size: 5779 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2917 2917 Size: 5779 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5779 XmaI C'CCGG_G Cuts at: 1870 3104 1870 Size: 1234 4545 XmnI GAAnn'nnTTC Cuts at: 2087 3908 2087 Size: 1821 3958 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3587 276 Size: 53 83 186 936 2053 2468 Fragments arranged by size: 2468 2053 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BplI BsiWI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NruI PacI PspXI RsrII SanDI SexAI SfiI SrfI SwaI