(Circular) (Six-base) MAPSORT of: pFN21AB5527.seq Check: 8447 from: 1 to: 6973 pFN21AB5527 6973 bp With 182 enzymes: SgfI * October 20, 2009 10:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3225 3225 Size: 6973 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4783 278 Size: 53 83 186 936 3247 2468 Fragments arranged by size: 3247 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2886 4316 1937 Size: 949 1430 4594 Fragments arranged by size: 4594 1430 949 Acc65I G'GTAC_C Cuts at: 4294 4294 Size: 6973 AclI AA'CG_TT Cuts at: 1563 5100 5473 1563 Size: 3537 373 3063 Fragments arranged by size: 3537 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2093 2111 2531 2542 2848 3003 3317 3335 Size: 461 18 420 11 306 155 314 18 Cuts at: 3335 3833 5036 6420 6688 1632 Size: 498 1203 1384 268 1917 Fragments arranged by size: 1917 1384 1203 498 461 420 314 306 268 155 18 18 11 AfeI AGC'GCT Cuts at: 2123 2774 2123 Size: 651 6322 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6776 AflIII A'CryG_T Cuts at: 1203 3765 5872 6849 1203 Size: 2562 2107 977 1327 Fragments arranged by size: 2562 2107 1327 977 AgeI A'CCGG_T Cuts at: 5804 5804 Size: 6973 AhdI GACnn_n'nnGTC Cuts at: 5702 5702 Size: 6973 AleI CACnn'nnGTG Cuts at: 1166 2880 3377 1166 Size: 1714 497 4762 Fragments arranged by size: 4762 1714 497 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2195 3237 1184 Size: 1011 1042 4920 Fragments arranged by size: 4920 1042 1011 AlwNI CAG_nnn'CTG Cuts at: 2068 2089 2747 3925 4716 6288 2068 Size: 21 658 1178 791 1572 2753 Fragments arranged by size: 2753 1572 1178 791 658 21 ApaI G_GGCC'C Cuts at: 2973 3048 3236 3876 2973 Size: 75 188 640 6070 Fragments arranged by size: 6070 640 188 75 ApaLI G'TGCA_C Cuts at: 5030 6186 5030 Size: 1156 5817 ApoI r'AATT_y Cuts at: 1493 3736 3949 4282 4508 4660 6769 1493 Size: 2243 213 333 226 152 2109 1697 Fragments arranged by size: 2243 2109 1697 333 226 213 152 AseI AT'TA_AT Cuts at: 160 1032 5527 160 Size: 872 4495 1606 Fragments arranged by size: 4495 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6973 AvaI C'yCGr_G Cuts at: 1870 1943 2011 2279 3329 4298 1870 Size: 73 68 268 1050 969 4545 Fragments arranged by size: 4545 1050 969 268 73 68 BamHI G'GATC_C Cuts at: 4303 4670 4303 Size: 367 6606 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2238 3213 3536 3639 3842 Size: 325 217 627 451 975 323 103 203 Cuts at: 3842 4294 5749 618 Size: 452 1455 1842 Fragments arranged by size: 1842 1455 975 627 452 451 325 323 217 203 103 BanII G_rGCy'C Cuts at: 1391 2520 2675 2784 2973 3048 3236 3339 3480 Size: 1129 155 109 189 75 188 103 141 Cuts at: 3480 3876 4292 1391 Size: 396 416 4072 Fragments arranged by size: 4072 1129 416 396 189 188 155 141 109 103 75 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2296 3396 928 Size: 971 397 1100 4505 Fragments arranged by size: 4505 1100 971 397 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2525 2559 5164 5198 1903 Size: 34 588 34 2605 34 3678 Fragments arranged by size: 3678 2605 588 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4184 4865 6075 4184 Size: 681 1210 5082 Fragments arranged by size: 5082 1210 681 BclI T'GATC_A Cuts at: 1548 1548 Size: 6973 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3140 5584 136 Size: 107 122 71 631 605 1468 2444 1525 Fragments arranged by size: 2444 1525 1468 631 605 122 107 71 BglII A'GATC_T Cuts at: 2553 4257 6968 2553 Size: 1704 2711 2558 Fragments arranged by size: 2711 2558 1704 BlpI GC'TnA_GC Cuts at: 2909 4390 2909 Size: 1481 5492 Bme1580I G_kGCm'C Cuts at: 1790 2241 2280 2973 3001 3048 3236 3589 3642 Size: 451 39 693 28 47 188 353 53 Cuts at: 3642 3876 3886 5034 6190 1790 Size: 234 10 1148 1156 2573 Fragments arranged by size: 2573 1156 1148 693 451 353 234 188 53 47 39 28 10 BmgBI CAC'GTC Cuts at: 1318 3766 4003 1318 Size: 2448 237 4288 Fragments arranged by size: 4288 2448 237 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2374 4704 5662 Size: 416 53 83 399 24 957 2330 958 Cuts at: 5662 442 Size: 1753 Fragments arranged by size: 2330 1753 958 957 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2966 3470 1056 Size: 1910 504 4559 Fragments arranged by size: 4559 1910 504 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2276 2612 2972 3044 3191 3209 3369 3511 Size: 1037 336 360 72 147 18 160 142 Cuts at: 3511 4250 5633 1239 Size: 739 1383 2579 Fragments arranged by size: 2579 1383 1037 739 360 336 160 147 142 72 18 Bpu10I CC'TnA_GC Cuts at: 2674 3113 3288 3461 3644 3867 2674 Size: 439 175 173 183 223 5780 Fragments arranged by size: 5780 439 223 183 175 173 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4166 5098 5963 6261 4166 Size: 932 865 298 4878 Fragments arranged by size: 4878 932 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3825 5636 882 Size: 617 2326 1811 2219 Fragments arranged by size: 2326 2219 1811 617 BsaAI yAC'GTr Cuts at: 493 1204 3808 4764 493 Size: 711 2604 956 2702 Fragments arranged by size: 2702 2604 956 711 BsaBI GATnn'nnATC Cuts at: 2055 6967 2055 Size: 4912 2061 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3143 3566 4780 5162 Size: 53 83 186 936 1610 423 1214 382 Cuts at: 5162 5797 275 Size: 635 1451 Fragments arranged by size: 1610 1451 1214 936 635 423 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5406 5804 6078 6225 6636 5406 Size: 398 274 147 411 5743 Fragments arranged by size: 5743 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2387 2417 2672 2702 4055 Size: 30 165 30 984 30 255 30 1353 Cuts at: 4055 4085 1178 Size: 30 4066 Fragments arranged by size: 4066 1353 984 255 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2128 2615 2747 3439 3670 4123 1186 Size: 942 487 132 692 231 453 4036 Fragments arranged by size: 4036 942 692 487 453 231 132 BseYI C'CCAG_C Cuts at: 2196 2358 2371 2631 2783 2972 3036 6176 6672 Size: 162 13 260 152 189 64 3140 496 Cuts at: 6672 2196 Size: 2497 Fragments arranged by size: 3140 2497 496 260 189 162 152 64 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2018 2363 3166 3455 1163 Size: 573 282 345 803 289 4681 Fragments arranged by size: 4681 803 573 345 289 282 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2650 4423 5184 6212 1540 Size: 275 186 649 1773 761 1028 2301 Fragments arranged by size: 2301 1773 1028 761 649 275 186 BsiHKAI G_wGCw'C Cuts at: 2520 3114 3480 5034 5119 6190 2520 Size: 594 366 1554 85 1071 3303 Fragments arranged by size: 3303 1554 1071 594 366 85 BsmI GAATG_Cn' Cuts at: 4484 4577 4484 Size: 93 6880 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2241 2280 2520 2675 2784 2973 3001 Size: 399 451 39 240 155 109 189 28 Cuts at: 3001 3048 3114 3236 3339 3480 3589 3642 3876 Size: 47 66 122 103 141 109 53 234 Cuts at: 3876 3886 4292 5034 5119 6190 1391 Size: 10 406 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 451 406 399 240 234 189 155 141 122 109 109 103 85 66 53 47 39 28 10 BspEI T'CCGG_A Cuts at: 6636 6636 Size: 6973 BspHI T'CATG_A Cuts at: 4862 6592 4862 Size: 1730 5243 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2955 3225 4328 844 Size: 2111 270 1103 3489 Fragments arranged by size: 3489 2111 1103 270 BsrBI CCG'CTC Cuts at: 1394 1823 4860 1394 Size: 429 3037 3507 Fragments arranged by size: 3507 3037 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5468 5642 65 Size: 1183 4220 174 1396 Fragments arranged by size: 4220 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5617 5804 1700 Size: 110 3807 187 2869 Fragments arranged by size: 3807 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6973 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6973 BssSI C'ACGA_G Cuts at: 5033 6045 5033 Size: 1012 5961 BstAPI GCAn_nnn'nTGC Cuts at: 794 2368 2747 3012 3925 794 Size: 1574 379 265 913 3842 Fragments arranged by size: 3842 1574 913 379 265 BstBI TT'CG_AA Cuts at: 5777 5777 Size: 6973 BstEII G'GTnAC_C Cuts at: 1184 3265 1184 Size: 2081 4892 BstXI CCAn_nnnn'nTGG Cuts at: 3749 3749 Size: 6973 BstYI r'GATC_y Cuts at: 1972 2056 2553 3093 3306 3981 4257 4303 4670 Size: 84 497 540 213 675 276 46 367 Cuts at: 4670 5055 5072 6524 6610 6622 6968 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 675 540 497 385 367 346 276 213 86 84 46 17 12 Bsu36I CC'TnA_GG Cuts at: 3366 3366 Size: 6973 BtgI C'CryG_G Cuts at: 513 1065 2004 2406 2667 3070 3696 3985 4049 Size: 552 939 402 261 403 626 289 64 Cuts at: 4049 513 Size: 3437 Fragments arranged by size: 3437 939 626 552 403 402 289 261 64 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2320 2328 3120 487 Size: 683 56 1094 8 792 4340 Fragments arranged by size: 4340 1094 792 683 56 8 BtsI GCAGTG_nn' Cuts at: 3178 3426 4489 5283 5303 3178 Size: 248 1063 794 20 4848 Fragments arranged by size: 4848 1063 794 248 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6973 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4231 4266 529 Size: 35 3667 35 3236 Fragments arranged by size: 3667 3236 35 35 DraI TTT'AAA Cuts at: 4277 4277 Size: 6973 DraIII CAC_nnn'GTG Cuts at: 2368 3634 2368 Size: 1266 5707 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2191 5980 809 Size: 507 875 3789 1802 Fragments arranged by size: 3789 1802 875 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4005 4153 4420 Size: 54 1425 50 275 18 2175 148 267 Cuts at: 4420 5309 6938 8 Size: 889 1629 43 Fragments arranged by size: 2175 1629 1425 889 275 267 148 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4420 1537 Size: 275 2608 4090 Fragments arranged by size: 4090 2608 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2935 3104 4903 1353 Size: 1582 169 1799 3423 Fragments arranged by size: 3423 1799 1582 169 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3614 5560 5934 6080 387 Size: 1064 138 2025 1946 374 146 1280 Fragments arranged by size: 2025 1946 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2518 3478 2518 Size: 960 6013 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2093 2111 2276 2531 2542 2612 2848 Size: 393 461 18 165 255 11 70 236 Cuts at: 2848 2972 3003 3044 3191 3209 3317 3335 3369 Size: 124 31 41 147 18 108 18 34 Cuts at: 3369 3511 3833 4250 5036 5633 6420 6688 1239 Size: 142 322 417 786 597 787 268 1524 Fragments arranged by size: 1524 787 786 597 461 417 393 322 268 255 236 165 147 142 124 108 70 41 34 31 18 18 18 11 EcoNI CCTnn'n_nnAGG Cuts at: 2951 3314 3866 2951 Size: 363 552 6058 Fragments arranged by size: 6058 552 363 EcoO109I rG'GnC_Cy Cuts at: 2744 3233 3795 3872 3873 2744 Size: 489 562 77 1 5844 Fragments arranged by size: 5844 562 489 77 1 EcoRI G'AATT_C Cuts at: 3949 4282 4660 3949 Size: 333 378 6262 Fragments arranged by size: 6262 378 333 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2503 2842 3115 3838 4162 2503 Size: 339 273 723 324 5314 Fragments arranged by size: 5314 723 339 324 273 FspI TGC'GCA Cuts at: 5479 5479 Size: 6973 HaeII r_GCGC'y Cuts at: 1678 2069 2125 2776 2862 6120 1678 Size: 391 56 651 86 3258 2531 Fragments arranged by size: 3258 2531 651 391 86 56 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2672 2704 3551 3583 4118 Size: 32 135 32 1100 32 847 32 535 Cuts at: 4118 4150 5614 5646 5688 5720 1373 Size: 32 1464 32 42 32 2626 Fragments arranged by size: 2626 1464 1100 847 535 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3937 4317 4563 669 Size: 988 281 1999 380 246 3079 Fragments arranged by size: 3079 1999 988 380 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6973 HpaI GTT'AAC Cuts at: 4563 4563 Size: 6973 KpnI G_GTAC'C Cuts at: 4298 4298 Size: 6973 MfeI C'AATT_G Cuts at: 3954 4572 3954 Size: 618 6355 MluI A'CGCG_T Cuts at: 6849 6849 Size: 6973 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3708 6087 6271 1305 Size: 2403 2379 184 2007 Fragments arranged by size: 2403 2379 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4007 4155 10 Size: 54 1425 343 2175 148 2828 Fragments arranged by size: 2828 2175 1425 343 148 54 MslI CAynn'nnrTG Cuts at: 518 1166 2528 2880 3219 3377 4931 5290 5449 Size: 648 1362 352 339 158 1554 359 159 Cuts at: 5449 518 Size: 2042 Fragments arranged by size: 2042 1554 1362 648 359 352 339 159 158 MspA1I CmG'CkG Cuts at: 1606 2200 2289 2351 2635 2669 2728 2806 3925 Size: 594 89 62 284 34 59 78 1119 Cuts at: 3925 4389 5066 6214 6459 1606 Size: 464 677 1148 245 2120 Fragments arranged by size: 2120 1148 1119 677 594 464 284 245 89 78 62 59 34 NcoI C'CATG_G Cuts at: 513 1065 2004 2406 3985 4049 513 Size: 552 939 402 1579 64 3437 Fragments arranged by size: 3437 1579 939 552 402 64 NdeI CA'TA_TG Cuts at: 387 387 Size: 6973 NheI G'CTAG_C Cuts at: 1052 2962 3466 1052 Size: 1910 504 4559 Fragments arranged by size: 4559 1910 504 NotI GC'GGCC_GC Cuts at: 4420 4420 Size: 6973 NspI r_CATG'y Cuts at: 1132 1229 3224 4331 5876 1132 Size: 97 1995 1107 1545 2229 Fragments arranged by size: 2229 1995 1545 1107 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6973 PciI A'CATG_T Cuts at: 5872 5872 Size: 6973 PflMI CCAn_nnn'nTGG Cuts at: 1108 2109 2638 2896 2920 3260 3634 3727 1108 Size: 1001 529 258 24 340 374 93 4354 Fragments arranged by size: 4354 1001 529 374 340 258 93 24 PfoI T'CCnGG_A Cuts at: 3123 3783 4266 3123 Size: 660 483 5830 Fragments arranged by size: 5830 660 483 PmeI GTTT'AAAC Cuts at: 4277 4277 Size: 6973 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5042 5074 6555 6587 5042 Size: 32 1481 32 5428 Fragments arranged by size: 5428 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2415 4022 1613 Size: 802 1607 4564 Fragments arranged by size: 4564 1607 802 PsiI TTA'TAA Cuts at: 4543 4543 Size: 6973 PspOMI G'GGCC_C Cuts at: 2969 3044 3232 3872 2969 Size: 75 188 640 6070 Fragments arranged by size: 6070 640 188 75 PstI C_TGCA'G Cuts at: 830 2502 2718 4325 830 Size: 1672 216 1607 3478 Fragments arranged by size: 3478 1672 1607 216 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6973 PvuII CAG'CTG Cuts at: 2200 2351 3925 2200 Size: 151 1574 5248 Fragments arranged by size: 5248 1574 151 SacI G_AGCT'C Cuts at: 2520 3480 2520 Size: 960 6013 SacII CC_GC'GG Cuts at: 2670 2670 Size: 6973 SalI G'TCGA_C Cuts at: 1936 4315 1936 Size: 2379 4594 SbfI CC_TGCA'GG Cuts at: 4325 4325 Size: 6973 ScaI AGT'ACT Cuts at: 5221 6933 5221 Size: 1712 5261 SexAI A'CCwGG_T Cuts at: 2919 2919 Size: 6973 SfcI C'TryA_G Cuts at: 826 1045 2042 2060 2426 2498 2714 3688 4321 Size: 219 997 18 366 72 216 974 633 Cuts at: 4321 5456 6137 6328 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 997 974 681 633 366 219 216 191 72 18 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6973 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6973 SmaI CCC'GGG Cuts at: 1872 4300 1872 Size: 2428 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4181 5077 5978 6240 820 Size: 197 926 2238 896 901 262 1553 Fragments arranged by size: 2238 1553 926 901 896 262 197 SnaBI TAC'GTA Cuts at: 493 4764 493 Size: 4271 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6973 SphI G_CATG'C Cuts at: 1132 3224 4331 1132 Size: 2092 1107 3774 Fragments arranged by size: 3774 2092 1107 SspI AAT'ATT Cuts at: 5 52 4897 5 Size: 47 4845 2081 Fragments arranged by size: 4845 2081 47 StuI AGG'CCT Cuts at: 1517 2612 3551 1517 Size: 1095 939 4939 Fragments arranged by size: 4939 1095 939 StyI C'CwwG_G Cuts at: 513 1065 2004 2406 2796 2817 3447 3985 4049 Size: 552 939 402 390 21 630 538 64 Cuts at: 4049 4412 513 Size: 363 3074 Fragments arranged by size: 3074 939 630 552 538 402 390 363 64 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2356 5000 5017 5170 5355 5800 325 Size: 1255 776 2644 17 153 185 445 1498 Fragments arranged by size: 2644 1498 1255 776 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2835 5219 6931 Size: 274 80 33 51 1443 858 2384 1712 Cuts at: 6931 96 Size: 138 Fragments arranged by size: 2384 1712 1443 858 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2387 2419 6933 6965 2387 Size: 32 4514 32 2395 Fragments arranged by size: 4514 2395 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2686 3488 1316 Size: 1370 802 4801 Fragments arranged by size: 4801 1370 802 XbaI T'CTAG_A Cuts at: 4309 4309 Size: 6973 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3973 3973 Size: 6973 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6973 XmaI C'CCGG_G Cuts at: 1870 4298 1870 Size: 2428 4545 XmnI GAAnn'nnTTC Cuts at: 2555 4145 5102 2555 Size: 1590 957 4426 Fragments arranged by size: 4426 1590 957 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4781 276 Size: 53 83 186 936 3247 2468 Fragments arranged by size: 3247 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SanDI SapI SfiI SfoI SrfI SwaI