Restriction map for
pFN21AB5445
##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BbsI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaI BsaWI
BseAI BsmAI BsmBI BsmI Bsp1407I
BspEI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I Bsu36I CelII
CpoI DraI DrdI Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoT22I
FbaI FspI HaeII HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MfeI MflI MluI MroI
MseI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tru9I Tsp509I Tth111I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 715 GACGTC GACGT^C
AfaI 513 GTAC GT^AC
AlwI 374 GGATC GGATC(4/5)
AvaII 33 GGACC G^G[AT]CC
BalI 257 TGGCCA TGG^CCA
BbvCI 612 CCTCAGC CCTCAGC(-5/-2)
BglI 562 GCCTTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 795 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 199 GAGGAG GAGGAG(10/8)
BsgI 484 GTGCAG GTGCAG(16/14)
BsiWI 512 CGTACG C^GTACG
BspDI 262 ATCGAT AT^CGAT
BsrBI 327 CCGCTC CCGCTC(-3/-3)
ClaI 262 ATCGAT AT^CGAT
DraIII 619 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 402 GAAGAG CTCTTC(1/4)
EcoRV 265 GATATC GAT^ATC
FseI 417 GGCCGGCC GGCCGG^CC
HinfI 438 GAATC G^A[ACGT]TC
Ksp632I 402 GAAGAG CTCTTC(1/4)
MamI 795 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 257 TGGCCA TGG^CCA
MscI 257 TGGCCA TGG^CCA
MslI 468 CATGACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 209 GCATGC [GA]CATG^[CT]
PflMI 148 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 176 GACGCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 513 GTAC GT^AC
SapI 402 GAAGAGC GCTCTTC(1/4)
SexAI 620 ACCTGGT A^CC[AT]GGT
SmaI 29 CCCGGG CCC^GGG
SphI 209 GCATGC GCATG^C
StuI 122 AGGCCT AGG^CCT
TaqI 263 TCGA T^CGA
TfiI 438 GAATC G^A[AT]TC
TthHB8I 263 TCGA T^CGA
Van91I 148 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 386 CCATCCTCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 29 CCCGGG C^CCGGG
XmaI 29 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwNI 187 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
783 CAGCACCTG
AspHI 430 GTGCTC G[AT]GC[AT]^C
655 GTGCTC
BanI 382 GGCACC G^G[CT][GA]CC
633 GGTGCC
BpmI 745 CTCCAG CTGGAG(16/14)
780 CTCCAG
BsiEI 569 CGGCCG CG[GA][CT]^CG
696 CGGCCG
BsiHKAI 430 GTGCTC G[AT]GC[AT]^C
655 GTGCTC
BspHI 434 TCATGA T^CATGA
467 TCATGA
BspMI 150 ACCTGC ACCTGC(4/8)
787 ACCTGC
BsrFI 418 GCCGGC [GA]^CCGG[CT]
478 GCCGGC
BstXI 278 CCAAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
527 CCAGCCTCCTGG
Cfr10I 418 GCCGGC [GA]^CCGG[CT]
478 GCCGGC
DdeI 59 CTGAG C^T[ACGT]AG
613 CTCAG
DpnII 375 GATC ^GATC
693 GATC
DraII 92 GGGGCCC [GA]G^G[ACGT]CC[CT]
662 GGGCCCT
EagI 569 CGGCCG C^GGCCG
696 CGGCCG
EclXI 569 CGGCCG C^GGCCG
696 CGGCCG
Eco52I 569 CGGCCG C^GGCCG
696 CGGCCG
EcoO109I 92 GGGGCCC [GA]G^G[ACGT]CC[CT]
662 GGGCCCT
HgaI 176 GACGC GACGC(5/10)
310 GCGTC
MboI 375 GATC ^GATC
693 GATC
MboII 402 GAAGA GAAGA(8/7)
608 TCTTC
MspA1I 190 CAGCTG C[AC]G^C[GT]G
214 CAGCTG
NaeI 418 GCCGGC GCC^GGC
478 GCCGGC
NdeII 375 GATC ^GATC
693 GATC
NgoMIV 418 GCCGGC G^CCGGC
478 GCCGGC
PvuII 190 CAGCTG CAG^CTG
214 CAGCTG
RcaI 434 TCATGA T^CATGA
467 TCATGA
Sau3AI 375 GATC ^GATC
693 GATC
TspRI 321 CAGTG CA[GC]TG[ACGT][ACGT]^
413 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AcyI AluI ApaI
AvaI BanII BbvI BcnI BmyI
BsaHI BsaJI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DsaI EaeI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HpaII HphI ItaI
MaeII MaeIII MnlI MspI MvaI
MvnI MwoI NciI NcoI NlaIII
NlaIV Sau96I ScrFI SfaNI StyI
TseI Tsp45I