(Circular) (Six-base) MAPSORT of: pFN21AB5445.seq Check: 8950 from: 1 to: 5518 pFN21AB5445 5518 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2164 2801 2164 Size: 637 4881 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2724 3328 278 Size: 53 83 186 936 1188 604 2468 Fragments arranged by size: 2468 1188 936 604 186 83 53 AccI GT'mk_AC Cuts at: 1937 2861 1937 Size: 924 4594 Acc65I G'GTAC_C Cuts at: 2839 2839 Size: 5518 AclI AA'CG_TT Cuts at: 1563 3645 4018 1563 Size: 2082 373 3063 Fragments arranged by size: 3063 2082 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3581 4965 5233 1632 Size: 1949 1384 268 1917 Fragments arranged by size: 1949 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5321 AflIII A'CryG_T Cuts at: 1203 4417 5394 1203 Size: 3214 977 1327 Fragments arranged by size: 3214 1327 977 AgeI A'CCGG_T Cuts at: 4349 4349 Size: 5518 AhdI GACnn_n'nnGTC Cuts at: 4247 4247 Size: 5518 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5518 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5518 AlwNI CAG_nnn'CTG Cuts at: 2197 2793 3261 4833 2197 Size: 596 468 1572 2882 Fragments arranged by size: 2882 1572 596 468 ApaI G_GGCC'C Cuts at: 2029 2102 2138 2671 2029 Size: 73 36 533 4876 Fragments arranged by size: 4876 533 73 36 ApaLI G'TGCA_C Cuts at: 3575 4731 3575 Size: 1156 4362 ApoI r'AATT_y Cuts at: 1493 2827 3053 3205 5314 1493 Size: 1334 226 152 2109 1697 Fragments arranged by size: 2109 1697 1334 226 152 AseI AT'TA_AT Cuts at: 160 1032 4072 160 Size: 872 3040 1606 Fragments arranged by size: 3040 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5518 AvaI C'yCGr_G Cuts at: 1870 1943 2034 2116 2131 2843 1870 Size: 73 91 82 15 712 4545 Fragments arranged by size: 4545 712 91 82 73 15 BamHI G'GATC_C Cuts at: 2848 3215 2848 Size: 367 5151 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2387 2638 2839 4294 618 Size: 325 217 627 600 251 201 1455 1842 Fragments arranged by size: 1842 1455 627 600 325 251 217 201 BanII G_rGCy'C Cuts at: 1391 2029 2102 2109 2138 2531 2671 2837 1391 Size: 638 73 7 29 393 140 166 4072 Fragments arranged by size: 4072 638 393 166 140 73 29 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4547 BbvCI CC'TCA_GC Cuts at: 2618 2618 Size: 5518 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3709 3743 1903 Size: 34 50 34 1688 34 3678 Fragments arranged by size: 3678 1688 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3410 4620 3410 Size: 1210 4308 BclI T'GATC_A Cuts at: 1548 1548 Size: 5518 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2573 4129 136 Size: 107 122 71 631 605 901 1556 1525 Fragments arranged by size: 1556 1525 901 631 605 122 107 71 BglII A'GATC_T Cuts at: 5513 5513 Size: 5518 BlpI GC'TnA_GC Cuts at: 2935 2935 Size: 5518 Bme1580I G_kGCm'C Cuts at: 1790 2029 2035 2102 2138 2332 2583 2643 2671 Size: 239 6 67 36 194 251 60 28 Cuts at: 2671 3579 4735 1790 Size: 908 1156 2573 Fragments arranged by size: 2573 1156 908 251 239 194 67 60 36 28 6 BmgBI CAC'GTC Cuts at: 1318 2713 1318 Size: 1395 4123 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3249 4207 442 Size: 416 53 83 399 24 1832 958 1753 Fragments arranged by size: 1832 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5518 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2735 2770 4178 1239 Size: 1496 35 1408 2579 Fragments arranged by size: 2579 1496 1408 35 Bpu10I CC'TnA_GC Cuts at: 2618 2618 Size: 5518 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3643 4508 4806 3643 Size: 865 298 4355 Fragments arranged by size: 4355 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4181 882 Size: 617 2682 2219 Fragments arranged by size: 2682 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3309 493 Size: 711 2105 2702 Fragments arranged by size: 2702 2105 711 BsaBI GATnn'nnATC Cuts at: 2804 5512 2804 Size: 2708 2810 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2182 2315 2721 3325 Size: 53 83 186 936 649 133 406 604 Cuts at: 3325 3707 4342 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 649 635 604 406 382 186 133 83 53 BsaWI w'CCGG_w Cuts at: 3951 4349 4623 4770 5181 3951 Size: 398 274 147 411 4288 Fragments arranged by size: 4288 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2711 2741 1178 Size: 30 165 30 1308 30 3955 Fragments arranged by size: 3955 1308 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2219 1186 Size: 1033 4485 BseYI C'CCAG_C Cuts at: 2290 2531 4721 5217 2290 Size: 241 2190 496 2591 Fragments arranged by size: 2591 2190 496 241 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2510 1163 Size: 573 774 4171 Fragments arranged by size: 4171 774 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2577 2704 2968 3729 4757 1540 Size: 275 186 576 127 264 761 1028 2301 Fragments arranged by size: 2301 1028 761 576 275 264 186 127 BsiHKAI G_wGCw'C Cuts at: 2439 2664 3579 3664 4735 2439 Size: 225 915 85 1071 3222 Fragments arranged by size: 3222 1071 915 225 85 BsiWI C'GTAC_G Cuts at: 2517 2517 Size: 5518 BsmI GAATG_Cn' Cuts at: 3029 3122 3029 Size: 93 5425 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2029 2035 2102 2109 2138 2332 2439 Size: 399 239 6 67 7 29 194 107 Cuts at: 2439 2531 2583 2643 2664 2671 2837 3579 3664 Size: 92 52 60 21 7 166 742 85 Cuts at: 3664 4735 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 239 194 166 107 92 85 67 60 52 29 21 7 7 6 BspEI T'CCGG_A Cuts at: 5181 5181 Size: 5518 BspHI T'CATG_A Cuts at: 2439 2472 3407 5137 2439 Size: 33 935 1730 2820 Fragments arranged by size: 2820 1730 935 33 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2164 2801 2873 844 Size: 1320 637 72 3489 Fragments arranged by size: 3489 1320 637 72 BsrBI CCG'CTC Cuts at: 1394 1823 2334 3405 1394 Size: 429 511 1071 3507 Fragments arranged by size: 3507 1071 511 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4013 4187 65 Size: 1183 2765 174 1396 Fragments arranged by size: 2765 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2423 2483 4162 4349 1700 Size: 110 613 60 1679 187 2869 Fragments arranged by size: 2869 1679 613 187 110 60 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5518 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5518 BssSI C'ACGA_G Cuts at: 3578 4590 3578 Size: 1012 4506 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5518 BstBI TT'CG_AA Cuts at: 4322 4322 Size: 5518 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5518 BstXI CCAn_nnnn'nTGG Cuts at: 2290 2539 2290 Size: 249 5269 BstYI r'GATC_y Cuts at: 1972 2848 3215 3600 3617 5069 5155 5167 5513 Size: 876 367 385 17 1452 86 12 346 Cuts at: 5513 1972 Size: 1977 Fragments arranged by size: 1977 1452 876 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2337 2365 2553 2598 513 Size: 552 1272 28 188 45 3433 Fragments arranged by size: 3433 1272 552 188 45 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2227 487 Size: 683 56 1001 3778 Fragments arranged by size: 3778 1001 683 56 BtsI GCAGTG_nn' Cuts at: 3034 3828 3848 3034 Size: 794 20 4704 Fragments arranged by size: 4704 794 20 ClaI AT'CG_AT Cuts at: 1554 2268 1554 Size: 714 4804 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5483 DraI TTT'AAA Cuts at: 2822 2822 Size: 5518 DraIII CAC_nnn'GTG Cuts at: 2629 2629 Size: 5518 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4525 809 Size: 507 3209 1802 Fragments arranged by size: 3209 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2262 2305 2421 Size: 54 1425 50 275 18 432 43 116 Cuts at: 2421 2425 2481 2574 2701 2965 3854 5483 8 Size: 4 56 93 127 264 889 1629 43 Fragments arranged by size: 1629 1425 889 432 275 264 127 116 93 56 54 50 43 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2574 2701 2965 1537 Size: 275 762 127 264 4090 Fragments arranged by size: 4090 762 275 264 127 EarI CTCTTCn'nnn_ Cuts at: 1353 2402 3448 1353 Size: 1049 1046 3423 Fragments arranged by size: 3423 1049 1046 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4105 4479 4625 387 Size: 1064 138 2516 374 146 1280 Fragments arranged by size: 2516 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2735 2770 3581 4178 4965 5233 1239 Size: 393 1103 35 811 597 787 268 1524 Fragments arranged by size: 1524 1103 811 787 597 393 268 35 EcoO109I rG'GnC_Cy Cuts at: 2098 2668 2098 Size: 570 4948 EcoRI G'AATT_C Cuts at: 2827 3205 2827 Size: 378 5140 EcoRV GAT'ATC Cuts at: 2272 2272 Size: 5518 FseI GG_CCGG'CC Cuts at: 2427 2427 Size: 5518 FspI TGC'GCA Cuts at: 4024 4024 Size: 5518 HaeII r_GCGC'y Cuts at: 1678 4665 1678 Size: 2987 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2057 2089 2376 2408 2711 Size: 32 135 32 485 32 287 32 303 Cuts at: 2711 2743 4159 4191 4233 4265 1373 Size: 32 1416 32 42 32 2626 Fragments arranged by size: 2626 1416 485 303 287 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2862 3108 669 Size: 988 281 924 246 3079 Fragments arranged by size: 3079 988 924 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5518 HpaI GTT'AAC Cuts at: 3108 3108 Size: 5518 KpnI G_GTAC'C Cuts at: 2843 2843 Size: 5518 MfeI C'AATT_G Cuts at: 3117 3117 Size: 5518 MluI A'CGCG_T Cuts at: 5394 5394 Size: 5518 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4632 4816 1305 Size: 3327 184 2007 Fragments arranged by size: 3327 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2264 10 Size: 54 1425 343 432 3264 Fragments arranged by size: 3264 1425 432 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2477 3476 3835 3994 518 Size: 648 1311 999 359 159 2042 Fragments arranged by size: 2042 1311 999 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2197 2221 2934 3611 4759 5004 1606 Size: 591 24 713 677 1148 245 2120 Fragments arranged by size: 2120 1148 713 677 591 245 24 NaeI GCC'GGC Cuts at: 2425 2485 2425 Size: 60 5458 NcoI C'CATG_G Cuts at: 513 1065 2337 2553 2598 513 Size: 552 1272 216 45 3433 Fragments arranged by size: 3433 1272 552 216 45 NdeI CA'TA_TG Cuts at: 387 387 Size: 5518 NgoMIV G'CCGG_C Cuts at: 2423 2483 2423 Size: 60 5458 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5518 NotI GC'GGCC_GC Cuts at: 2965 2965 Size: 5518 NspI r_CATG'y Cuts at: 1132 1229 2218 2876 4421 1132 Size: 97 989 658 1545 2229 Fragments arranged by size: 2229 1545 989 658 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5518 PciI A'CATG_T Cuts at: 4417 4417 Size: 5518 PflMI CCAn_nnn'nTGG Cuts at: 1108 2159 1108 Size: 1051 4467 PfoI T'CCnGG_A Cuts at: 2463 2538 2463 Size: 75 5443 PmeI GTTT'AAAC Cuts at: 2822 2822 Size: 5518 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3587 3619 5100 5132 3587 Size: 32 1481 32 3973 Fragments arranged by size: 3973 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2185 1613 Size: 572 4946 PsiI TTA'TAA Cuts at: 3088 3088 Size: 5518 PspOMI G'GGCC_C Cuts at: 2025 2098 2134 2667 2025 Size: 73 36 533 4876 Fragments arranged by size: 4876 533 73 36 PstI C_TGCA'G Cuts at: 830 2870 830 Size: 2040 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5518 PvuII CAG'CTG Cuts at: 2197 2221 2197 Size: 24 5494 SalI G'TCGA_C Cuts at: 1936 2860 1936 Size: 924 4594 SapI GCTCTTCn'nnn_ Cuts at: 2402 2402 Size: 5518 SbfI CC_TGCA'GG Cuts at: 2870 2870 Size: 5518 ScaI AGT'ACT Cuts at: 3766 5478 3766 Size: 1712 3806 SexAI A'CCwGG_T Cuts at: 2625 2625 Size: 5518 SfcI C'TryA_G Cuts at: 826 1045 2866 4001 4682 4873 826 Size: 219 1821 1135 681 191 1471 Fragments arranged by size: 1821 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5518 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5518 SmaI CCC'GGG Cuts at: 1872 2036 2845 1872 Size: 164 809 4545 Fragments arranged by size: 4545 809 164 SmlI C'TyrA_G Cuts at: 820 1017 1943 3622 4523 4785 820 Size: 197 926 1679 901 262 1553 Fragments arranged by size: 1679 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3309 493 Size: 2816 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5518 SphI G_CATG'C Cuts at: 1132 2218 2876 1132 Size: 1086 658 3774 Fragments arranged by size: 3774 1086 658 SspI AAT'ATT Cuts at: 5 52 3442 5 Size: 47 3390 2081 Fragments arranged by size: 3390 2081 47 StuI AGG'CCT Cuts at: 1517 2129 1517 Size: 612 4906 StyI C'CwwG_G Cuts at: 513 1065 2091 2337 2553 2598 2957 513 Size: 552 1026 246 216 45 359 3074 Fragments arranged by size: 3074 1026 552 359 246 216 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3545 3562 3715 3900 4345 325 Size: 1255 1965 17 153 185 445 1498 Fragments arranged by size: 1965 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3764 5476 96 Size: 274 80 33 51 1443 1787 1712 138 Fragments arranged by size: 1787 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5478 5510 5478 Size: 32 5486 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5518 XbaI T'CTAG_A Cuts at: 2854 2854 Size: 5518 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2398 2398 Size: 5518 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5518 XmaI C'CCGG_G Cuts at: 1870 2034 2843 1870 Size: 164 809 4545 Fragments arranged by size: 4545 809 164 XmnI GAAnn'nnTTC Cuts at: 3647 3647 Size: 5518 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2722 3326 276 Size: 53 83 186 936 1188 604 2468 Fragments arranged by size: 2468 1188 936 604 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsmBI BstZ17I Bsu36I EcoICRI EcoNI FalI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI