Restriction map for
pFN21AB5445
(Linear) (Six-base) MAPSORT of: KIBB5445.seq Check: 9474 from: 1 to: 813
KIBB5445.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 159 796 813
Size: 159 637 17
Fragments arranged by size:
637 159 17
AatII G_ACGT'C
Cuts at: 0 719 813
Size: 719 94
AlwNI CAG_nnn'CTG
Cuts at: 0 192 788 813
Size: 192 596 25
Fragments arranged by size:
596 192 25
ApaI G_GGCC'C
Cuts at: 0 24 97 133 666 813
Size: 24 73 36 533 147
Fragments arranged by size:
533 147 73 36 24
AvaI C'yCGr_G
Cuts at: 0 29 111 126 813
Size: 29 82 15 687
Fragments arranged by size:
687 82 29 15
BanI G'GyrC_C
Cuts at: 0 382 633 813
Size: 382 251 180
Fragments arranged by size:
382 251 180
BanII G_rGCy'C
Cuts at: 0 24 97 104 133 526 666 813
Size: 24 73 7 29 393 140 147
Fragments arranged by size:
393 147 140 73 29 24 7
BbvCI CC'TCA_GC
Cuts at: 0 613 813
Size: 613 200
BglI GCCn_nnn'nGGC
Cuts at: 0 568 813
Size: 568 245
Bme1580I G_kGCm'C
Cuts at: 0 24 30 97 133 327 578 638 666
Size: 24 6 67 36 194 251 60 28
Cuts at: 666 813
Size: 147
Fragments arranged by size:
251 194 147 67 60 36 28 24
6
BmgBI CAC'GTC
Cuts at: 0 708 813
Size: 708 105
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 765 813
Size: 730 35 48
Fragments arranged by size:
730 48 35
Bpu10I CC'TnA_GC
Cuts at: 0 613 813
Size: 613 200
BsaBI GATnn'nnATC
Cuts at: 0 799 813
Size: 799 14
BsaHI Gr'CG_yC
Cuts at: 0 177 310 716 813
Size: 177 133 406 97
Fragments arranged by size:
406 177 133 97
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 706 736 813
Size: 706 30 77
Fragments arranged by size:
706 77 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 214 813
Size: 214 599
BseYI C'CCAG_C
Cuts at: 0 285 526 813
Size: 285 241 287
Fragments arranged by size:
287 285 241
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 813
Size: 505 308
BsiEI CG_ry'CG
Cuts at: 0 572 699 813
Size: 572 127 114
Fragments arranged by size:
572 127 114
BsiHKAI G_wGCw'C
Cuts at: 0 434 659 813
Size: 434 225 154
Fragments arranged by size:
434 225 154
BsiWI C'GTAC_G
Cuts at: 0 512 813
Size: 512 301
Bsp1286I G_dGCh'C
Cuts at: 0 24 30 97 104 133 327 434 526
Size: 24 6 67 7 29 194 107 92
Cuts at: 526 578 638 659 666 813
Size: 52 60 21 7 147
Fragments arranged by size:
194 147 107 92 67 60 52 29
24 21 7 7 6
BspHI T'CATG_A
Cuts at: 0 434 467 813
Size: 434 33 346
Fragments arranged by size:
434 346 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 159 796 813
Size: 159 637 17
Fragments arranged by size:
637 159 17
BsrBI CCG'CTC
Cuts at: 0 329 813
Size: 329 484
BsrFI r'CCGG_y
Cuts at: 0 418 478 813
Size: 418 60 335
Fragments arranged by size:
418 335 60
BstXI CCAn_nnnn'nTGG
Cuts at: 0 285 534 813
Size: 285 249 279
Fragments arranged by size:
285 279 249
BtgI C'CryG_G
Cuts at: 0 332 360 548 593 813
Size: 332 28 188 45 220
Fragments arranged by size:
332 220 188 45 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 222 813
Size: 222 591
ClaI AT'CG_AT
Cuts at: 0 263 813
Size: 263 550
DraIII CAC_nnn'GTG
Cuts at: 0 624 813
Size: 624 189
EaeI y'GGCC_r
Cuts at: 0 257 300 416 420 476 569 696 813
Size: 257 43 116 4 56 93 127 117
Fragments arranged by size:
257 127 117 116 93 56 43 4
EagI C'GGCC_G
Cuts at: 0 569 696 813
Size: 569 127 117
Fragments arranged by size:
569 127 117
EarI CTCTTCn'nnn_
Cuts at: 0 397 813
Size: 397 416
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 765 813
Size: 730 35 48
Fragments arranged by size:
730 48 35
EcoO109I rG'GnC_Cy
Cuts at: 0 93 663 813
Size: 93 570 150
Fragments arranged by size:
570 150 93
EcoRV GAT'ATC
Cuts at: 0 267 813
Size: 267 546
FseI GG_CCGG'CC
Cuts at: 0 422 813
Size: 422 391
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 52 84 371 403 706 738 813
Size: 52 32 287 32 303 32 75
Fragments arranged by size:
303 287 75 52 32 32 32
MscI TGG'CCA
Cuts at: 0 259 813
Size: 259 554
MslI CAynn'nnrTG
Cuts at: 0 472 813
Size: 472 341
MspA1I CmG'CkG
Cuts at: 0 192 216 813
Size: 192 24 597
Fragments arranged by size:
597 192 24
NaeI GCC'GGC
Cuts at: 0 420 480 813
Size: 420 60 333
Fragments arranged by size:
420 333 60
NcoI C'CATG_G
Cuts at: 0 332 548 593 813
Size: 332 216 45 220
Fragments arranged by size:
332 220 216 45
NgoMIV G'CCGG_C
Cuts at: 0 418 478 813
Size: 418 60 335
Fragments arranged by size:
418 335 60
NspI r_CATG'y
Cuts at: 0 213 813
Size: 213 600
PflMI CCAn_nnn'nTGG
Cuts at: 0 154 813
Size: 154 659
PfoI T'CCnGG_A
Cuts at: 0 458 533 813
Size: 458 75 280
Fragments arranged by size:
458 280 75
PshAI GACnn'nnGTC
Cuts at: 0 180 813
Size: 180 633
PspOMI G'GGCC_C
Cuts at: 0 20 93 129 662 813
Size: 20 73 36 533 151
Fragments arranged by size:
533 151 73 36 20
PvuII CAG'CTG
Cuts at: 0 192 216 813
Size: 192 24 597
Fragments arranged by size:
597 192 24
SapI GCTCTTCn'nnn_
Cuts at: 0 397 813
Size: 397 416
SexAI A'CCwGG_T
Cuts at: 0 620 813
Size: 620 193
SmaI CCC'GGG
Cuts at: 0 31 813
Size: 31 782
SphI G_CATG'C
Cuts at: 0 213 813
Size: 213 600
StuI AGG'CCT
Cuts at: 0 124 813
Size: 124 689
StyI C'CwwG_G
Cuts at: 0 86 332 548 593 813
Size: 86 246 216 45 220
Fragments arranged by size:
246 220 216 86 45
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 393 813
Size: 393 420
XmaI C'CCGG_G
Cuts at: 0 29 813
Size: 29 784
ZraI GAC'GTC
Cuts at: 0 717 813
Size: 717 96
Enzymes that do cut:
AarI AatII AlwNI ApaI AvaI BanI BanII BbvCI
BglI Bme1580I BmgBI BpmI Bpu10I BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspHI
BspMI BsrBI BsrFI BstXI BtgI BtgZI ClaI DraIII
EaeI EagI EarI Eco57MI EcoO109I EcoRV FseI Hin4I
MscI MslI MspA1I NaeI NcoI NgoMIV NspI PflMI
PfoI PshAI PspOMI PvuII SapI SexAI SmaI SphI
StuI StyI XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BcgI BciVI
BclI BfrBI BglII BlpI BmrI BmtI BplI BpuEI
BsaI BsaAI BsaWI BsmI BsmBI BspEI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtsI CspCI DraI DrdI EciI EcoICRI EcoNI EcoRI
FalI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI NarI NdeI NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PpuMI PsiI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SrfI SspI SwaI
TaqII TatI TstI Tth111I XbaI XhoI XmnI