(Circular) (Six-base) MAPSORT of: pFN21AB5444.seq Check: 3559 from: 1 to: 6877 pFN21AB5444 6877 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3472 3472 Size: 6877 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4687 278 Size: 53 83 186 936 3151 2468 Fragments arranged by size: 3151 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2265 4220 1937 Size: 328 1955 4594 Fragments arranged by size: 4594 1955 328 Acc65I G'GTAC_C Cuts at: 2537 4198 2537 Size: 1661 5216 AclI AA'CG_TT Cuts at: 1563 5004 5377 1563 Size: 3441 373 3063 Fragments arranged by size: 3441 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2339 4940 6324 6592 1632 Size: 707 2601 1384 268 1917 Fragments arranged by size: 2601 1917 1384 707 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6680 AflIII A'CryG_T Cuts at: 1203 5776 6753 1203 Size: 4573 977 1327 Fragments arranged by size: 4573 1327 977 AgeI A'CCGG_T Cuts at: 5708 5708 Size: 6877 AhdI GACnn_n'nnGTC Cuts at: 5606 5606 Size: 6877 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6877 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6877 AlwNI CAG_nnn'CTG Cuts at: 4118 4620 6192 4118 Size: 502 1572 4803 Fragments arranged by size: 4803 1572 502 ApaLI G'TGCA_C Cuts at: 2018 4934 6090 2018 Size: 2916 1156 2805 Fragments arranged by size: 2916 2805 1156 ApoI r'AATT_y Cuts at: 1493 2912 2987 3287 3584 4186 4412 4564 6673 Size: 1419 75 300 297 602 226 152 2109 Cuts at: 6673 1493 Size: 1697 Fragments arranged by size: 2109 1697 1419 602 300 297 226 152 75 AseI AT'TA_AT Cuts at: 160 1032 5431 160 Size: 872 4399 1606 Fragments arranged by size: 4399 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6877 AvaI C'yCGr_G Cuts at: 1870 1943 3177 4136 4202 1870 Size: 73 1234 959 66 4545 Fragments arranged by size: 4545 1234 959 73 66 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2529 2562 3104 3137 2529 Size: 33 542 33 6269 Fragments arranged by size: 6269 542 33 33 BamHI G'GATC_C Cuts at: 4018 4207 4574 4018 Size: 189 367 6321 Fragments arranged by size: 6321 367 189 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2537 4198 5653 618 Size: 325 217 627 750 1661 1455 1842 Fragments arranged by size: 1842 1661 1455 750 627 325 217 BanII G_rGCy'C Cuts at: 1391 2526 3022 3249 3552 4196 1391 Size: 1135 496 227 303 644 4072 Fragments arranged by size: 4072 1135 644 496 303 227 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2472 2949 3273 3339 3753 928 Size: 971 573 477 324 66 414 4052 Fragments arranged by size: 4052 971 573 477 414 324 66 BbvCI CC'TCA_GC Cuts at: 3930 3930 Size: 6877 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4133 4167 5068 5102 1903 Size: 34 50 34 2112 34 901 34 3678 Fragments arranged by size: 3678 2112 901 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4769 5979 4769 Size: 1210 5667 BclI T'GATC_A Cuts at: 1548 2439 2836 4053 1548 Size: 891 397 1217 4372 Fragments arranged by size: 4372 1217 891 397 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2057 5488 136 Size: 107 122 71 631 605 385 3431 1525 Fragments arranged by size: 3431 1525 631 605 385 122 107 71 BglII A'GATC_T Cuts at: 6872 6872 Size: 6877 BlpI GC'TnA_GC Cuts at: 4294 4294 Size: 6877 Bme1580I G_kGCm'C Cuts at: 1790 2022 4938 6094 1790 Size: 232 2916 1156 2573 Fragments arranged by size: 2916 2573 1156 232 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6877 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2947 3379 4608 Size: 416 53 83 399 24 1530 432 1229 Cuts at: 4608 5566 442 Size: 958 1753 Fragments arranged by size: 1753 1530 1229 958 432 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6877 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2152 3203 3268 3319 2152 Size: 1051 65 51 5710 Fragments arranged by size: 5710 1051 65 51 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2508 3173 3411 3647 3902 5537 1239 Size: 1269 665 238 236 255 1635 2579 Fragments arranged by size: 2579 1635 1269 665 255 238 236 Bpu10I CC'TnA_GC Cuts at: 3930 3930 Size: 6877 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3263 3299 3563 4034 5002 5867 6165 3263 Size: 36 264 471 968 865 298 3975 Fragments arranged by size: 3975 968 865 471 298 264 36 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3610 5540 882 Size: 617 2111 1930 2219 Fragments arranged by size: 2219 2111 1930 617 BsaAI yAC'GTr Cuts at: 493 1204 4668 493 Size: 711 3464 2702 Fragments arranged by size: 3464 2702 711 BsaBI GATnn'nnATC Cuts at: 6871 6871 Size: 6877 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4684 5066 5701 275 Size: 53 83 186 936 3151 382 635 1451 Fragments arranged by size: 3151 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5310 5708 5982 6129 6540 5310 Size: 398 274 147 411 5647 Fragments arranged by size: 5647 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3320 3350 1178 Size: 30 165 30 1917 30 4705 Fragments arranged by size: 4705 1917 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2045 2048 2789 2915 3659 3662 1186 Size: 859 3 741 126 744 3 4401 Fragments arranged by size: 4401 859 744 741 126 3 3 BseYI C'CCAG_C Cuts at: 6080 6576 6080 Size: 496 6381 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2385 3590 1163 Size: 573 649 1205 4450 Fragments arranged by size: 4450 1205 649 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4327 5088 6116 1540 Size: 275 186 2326 761 1028 2301 Fragments arranged by size: 2326 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2022 3249 4938 5023 6094 2022 Size: 1227 1689 85 1071 2805 Fragments arranged by size: 2805 1689 1227 1071 85 BsmI GAATG_Cn' Cuts at: 2339 2617 3299 4388 4481 2339 Size: 278 682 1089 93 4735 Fragments arranged by size: 4735 1089 682 278 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3905 4161 3905 Size: 256 6621 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2526 3022 3249 3552 4196 4938 Size: 399 232 504 496 227 303 644 742 Cuts at: 4938 5023 6094 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 644 504 496 399 303 232 227 85 BspEI T'CCGG_A Cuts at: 6540 6540 Size: 6877 BspHI T'CATG_A Cuts at: 3702 3819 4766 6496 3702 Size: 117 947 1730 4083 Fragments arranged by size: 4083 1730 947 117 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3472 4232 844 Size: 2628 760 3489 Fragments arranged by size: 3489 2628 760 BsrBI CCG'CTC Cuts at: 1394 1823 3814 4764 1394 Size: 429 1991 950 3507 Fragments arranged by size: 3507 1991 950 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2316 2996 5372 5546 65 Size: 1183 1068 680 2376 174 1396 Fragments arranged by size: 2376 1396 1183 1068 680 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5521 5708 1700 Size: 110 3711 187 2869 Fragments arranged by size: 3711 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6877 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6877 BssSI C'ACGA_G Cuts at: 4937 5949 4937 Size: 1012 5865 BstAPI GCAn_nnn'nTGC Cuts at: 794 2066 794 Size: 1272 5605 BstBI TT'CG_AA Cuts at: 2192 5681 2192 Size: 3489 3388 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6877 BstYI r'GATC_y Cuts at: 1972 3157 4018 4125 4207 4574 4959 4976 6428 Size: 1185 861 107 82 367 385 17 1452 Cuts at: 6428 6514 6526 6872 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1185 861 385 367 346 107 86 82 17 12 Bsu36I CC'TnA_GG Cuts at: 2010 2241 3636 2010 Size: 231 1395 5251 Fragments arranged by size: 5251 1395 231 BtgI C'CryG_G Cuts at: 513 1065 2023 2515 3136 3852 513 Size: 552 958 492 621 716 3538 Fragments arranged by size: 3538 958 716 621 552 492 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4158 487 Size: 683 56 2932 3206 Fragments arranged by size: 3206 2932 683 56 BtsI GCAGTG_nn' Cuts at: 4393 5187 5207 4393 Size: 794 20 6063 Fragments arranged by size: 6063 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6877 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6842 DraI TTT'AAA Cuts at: 4181 4181 Size: 6877 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3409 3445 5884 809 Size: 507 2093 36 2439 1802 Fragments arranged by size: 2439 2093 1802 507 36 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3486 3867 4324 Size: 54 1425 50 275 18 1656 381 457 Cuts at: 4324 5213 6842 8 Size: 889 1629 43 Fragments arranged by size: 1656 1629 1425 889 457 381 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4324 1537 Size: 275 2512 4090 Fragments arranged by size: 4090 2512 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2358 2580 2694 2712 3254 3590 3660 4807 Size: 1005 222 114 18 542 336 70 1147 Cuts at: 4807 1353 Size: 3423 Fragments arranged by size: 3423 1147 1005 542 336 222 114 70 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3065 5464 5838 5984 387 Size: 1064 138 1476 2399 374 146 1280 Fragments arranged by size: 2399 1476 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3247 3247 Size: 6877 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2339 2508 3173 3411 3647 3902 4940 Size: 393 707 169 665 238 236 255 1038 Cuts at: 4940 5537 6324 6592 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1038 787 707 665 597 393 268 255 238 236 169 EcoO109I rG'GnC_Cy Cuts at: 3961 3961 Size: 6877 EcoRI G'AATT_C Cuts at: 2912 2987 4186 4564 2912 Size: 75 1199 378 5225 Fragments arranged by size: 5225 1199 378 75 FspI TGC'GCA Cuts at: 5383 5383 Size: 6877 HaeII r_GCGC'y Cuts at: 1678 6024 1678 Size: 4346 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2539 2571 3389 3391 3421 Size: 32 135 32 967 32 818 2 30 Cuts at: 3421 3423 4117 4139 4149 4171 5518 5550 5592 Size: 2 694 22 10 22 1347 32 42 Cuts at: 5592 5624 1373 Size: 32 2626 Fragments arranged by size: 2626 1347 967 818 694 135 42 32 32 32 32 32 30 22 22 10 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 3148 4221 4467 669 Size: 988 281 1210 1073 246 3079 Fragments arranged by size: 3079 1210 1073 988 281 246 HindIII A'AGCT_T Cuts at: 748 3317 748 Size: 2569 4308 HpaI GTT'AAC Cuts at: 4467 4467 Size: 6877 KpnI G_GTAC'C Cuts at: 2541 4202 2541 Size: 1661 5216 MfeI C'AATT_G Cuts at: 4476 4476 Size: 6877 MluI A'CGCG_T Cuts at: 6753 6753 Size: 6877 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2130 2178 2964 4059 5991 6175 1305 Size: 825 48 786 1095 1932 184 2007 Fragments arranged by size: 2007 1932 1095 825 786 184 48 MscI TGG'CCA Cuts at: 10 64 1489 1832 3488 3869 10 Size: 54 1425 343 1656 381 3018 Fragments arranged by size: 3018 1656 1425 381 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2279 2375 2454 4835 5194 5353 518 Size: 648 1113 96 79 2381 359 159 2042 Fragments arranged by size: 2381 2042 1113 648 359 159 96 79 MspA1I CmG'CkG Cuts at: 1606 2787 3099 4293 4970 6118 6363 1606 Size: 1181 312 1194 677 1148 245 2120 Fragments arranged by size: 2120 1194 1181 1148 677 312 245 NcoI C'CATG_G Cuts at: 513 1065 2023 2515 3852 513 Size: 552 958 492 1337 3538 Fragments arranged by size: 3538 1337 958 552 492 NdeI CA'TA_TG Cuts at: 387 387 Size: 6877 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6877 NotI GC'GGCC_GC Cuts at: 4324 4324 Size: 6877 NspI r_CATG'y Cuts at: 1132 1229 4235 5780 1132 Size: 97 3006 1545 2229 Fragments arranged by size: 3006 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6877 PciI A'CATG_T Cuts at: 5776 5776 Size: 6877 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6877 PmeI GTTT'AAAC Cuts at: 4181 4181 Size: 6877 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4946 4978 6459 6491 4946 Size: 32 1481 32 5332 Fragments arranged by size: 5332 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3961 3961 Size: 6877 PshAI GACnn'nnGTC Cuts at: 1613 3757 1613 Size: 2144 4733 PsiI TTA'TAA Cuts at: 4447 4447 Size: 6877 PstI C_TGCA'G Cuts at: 830 4114 4229 830 Size: 3284 115 3478 Fragments arranged by size: 3478 3284 115 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6877 PvuII CAG'CTG Cuts at: 3099 3099 Size: 6877 SacI G_AGCT'C Cuts at: 3249 3249 Size: 6877 SalI G'TCGA_C Cuts at: 1936 4219 1936 Size: 2283 4594 SapI GCTCTTCn'nnn_ Cuts at: 2358 3254 3590 2358 Size: 896 336 5645 Fragments arranged by size: 5645 896 336 SbfI CC_TGCA'GG Cuts at: 4229 4229 Size: 6877 ScaI AGT'ACT Cuts at: 3563 5125 6837 3563 Size: 1562 1712 3603 Fragments arranged by size: 3603 1712 1562 SexAI A'CCwGG_T Cuts at: 3963 3963 Size: 6877 SfcI C'TryA_G Cuts at: 826 1045 2970 4110 4225 5360 6041 6232 826 Size: 219 1925 1140 115 1135 681 191 1471 Fragments arranged by size: 1925 1471 1140 1135 681 219 191 115 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6877 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6877 SmaI CCC'GGG Cuts at: 1872 4204 1872 Size: 2332 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3242 3314 3578 4013 4136 4981 Size: 197 926 1299 72 264 435 123 845 Cuts at: 4981 5882 6144 820 Size: 901 262 1553 Fragments arranged by size: 1553 1299 926 901 845 435 264 262 197 123 72 SnaBI TAC'GTA Cuts at: 493 4668 493 Size: 4175 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6877 SphI G_CATG'C Cuts at: 1132 4235 1132 Size: 3103 3774 SspI AAT'ATT Cuts at: 5 52 4801 5 Size: 47 4749 2081 Fragments arranged by size: 4749 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6877 StyI C'CwwG_G Cuts at: 513 1065 2023 2154 2515 3615 3852 4316 513 Size: 552 958 131 361 1100 237 464 3074 Fragments arranged by size: 3074 1100 958 552 464 361 237 131 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2909 3560 4904 4921 5074 5259 5704 Size: 1255 1329 651 1344 17 153 185 445 Cuts at: 5704 325 Size: 1498 Fragments arranged by size: 1498 1344 1329 1255 651 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3561 5123 6835 Size: 274 80 33 51 1443 1584 1562 1712 Cuts at: 6835 96 Size: 138 Fragments arranged by size: 1712 1584 1562 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3320 3352 6837 6869 3320 Size: 32 3485 32 3328 Fragments arranged by size: 3485 3328 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6877 XbaI T'CTAG_A Cuts at: 4213 4213 Size: 6877 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2903 3622 3877 2903 Size: 719 255 5903 Fragments arranged by size: 5903 719 255 XhoI C'TCGA_G Cuts at: 1943 4136 1943 Size: 2193 4684 XmaI C'CCGG_G Cuts at: 1870 4202 1870 Size: 2332 4545 XmnI GAAnn'nnTTC Cuts at: 5006 5006 Size: 6877 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4685 276 Size: 53 83 186 936 3151 2468 Fragments arranged by size: 3151 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI