(Circular) (Six-base) MAPSORT of: pFN21AB5378.seq Check: 1270 from: 1 to: 6922 pFN21AB5378 6922 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3034 3034 Size: 6922 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4732 278 Size: 53 83 186 936 3196 2468 Fragments arranged by size: 3196 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4265 1937 Size: 2328 4594 Acc65I G'GTAC_C Cuts at: 4243 4243 Size: 6922 AclI AA'CG_TT Cuts at: 1563 5049 5422 1563 Size: 3486 373 3063 Fragments arranged by size: 3486 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2063 2487 2673 2847 3273 3831 4985 6369 Size: 431 424 186 174 426 558 1154 1384 Cuts at: 6369 6637 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1154 558 431 426 424 268 186 174 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6725 AflIII A'CryG_T Cuts at: 1203 2139 2217 5821 6798 1203 Size: 936 78 3604 977 1327 Fragments arranged by size: 3604 1327 977 936 78 AgeI A'CCGG_T Cuts at: 5753 5753 Size: 6922 AhdI GACnn_n'nnGTC Cuts at: 5651 5651 Size: 6922 AleI CACnn'nnGTG Cuts at: 1166 3077 3203 3692 1166 Size: 1911 126 489 4396 Fragments arranged by size: 4396 1911 489 126 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6922 AlwNI CAG_nnn'CTG Cuts at: 3271 4665 6237 3271 Size: 1394 1572 3956 Fragments arranged by size: 3956 1572 1394 ApaLI G'TGCA_C Cuts at: 2221 4979 6135 2221 Size: 2758 1156 3008 Fragments arranged by size: 3008 2758 1156 ApoI r'AATT_y Cuts at: 1493 2559 3351 3956 4231 4457 4609 6718 1493 Size: 1066 792 605 275 226 152 2109 1697 Fragments arranged by size: 2109 1697 1066 792 605 275 226 152 AseI AT'TA_AT Cuts at: 160 1032 5476 160 Size: 872 4444 1606 Fragments arranged by size: 4444 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6922 AvaI C'yCGr_G Cuts at: 1870 1943 4247 1870 Size: 73 2304 4545 Fragments arranged by size: 4545 2304 73 BamHI G'GATC_C Cuts at: 2518 3643 4252 4619 2518 Size: 1125 609 367 4821 Fragments arranged by size: 4821 1125 609 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2477 3694 4243 5698 618 Size: 325 217 627 690 1217 549 1455 1842 Fragments arranged by size: 1842 1455 1217 690 627 549 325 217 BanII G_rGCy'C Cuts at: 1391 2880 2979 3856 4241 1391 Size: 1489 99 877 385 4072 Fragments arranged by size: 4072 1489 877 385 99 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3170 928 Size: 971 1271 4680 Fragments arranged by size: 4680 1271 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3051 3085 5113 5147 1903 Size: 34 50 34 1030 34 2028 34 3678 Fragments arranged by size: 3678 2028 1030 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4814 6024 4814 Size: 1210 5712 BclI T'GATC_A Cuts at: 1548 1548 Size: 6922 BfrBI ATG'CAT Cuts at: 2446 2446 Size: 6922 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3719 5533 136 Size: 107 122 71 631 605 2047 1814 1525 Fragments arranged by size: 2047 1814 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2245 2910 4125 6917 2245 Size: 665 1215 2792 2250 Fragments arranged by size: 2792 2250 1215 665 BlpI GC'TnA_GC Cuts at: 3911 4339 3911 Size: 428 6494 Bme1580I G_kGCm'C Cuts at: 1790 2225 2482 4983 6139 1790 Size: 435 257 2501 1156 2573 Fragments arranged by size: 2573 2501 1156 435 257 BmgBI CAC'GTC Cuts at: 1318 2029 1318 Size: 711 6211 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3727 4653 5611 Size: 416 53 83 399 24 2310 926 958 Cuts at: 5611 442 Size: 1753 Fragments arranged by size: 2310 1753 958 926 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2852 1056 Size: 1796 5126 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3870 3959 5582 1239 Size: 2631 89 1623 2579 Fragments arranged by size: 2631 2579 1623 89 Bpu10I CC'TnA_GC Cuts at: 4059 4059 Size: 6922 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2112 2563 2720 5047 5912 6210 2112 Size: 451 157 2327 865 298 2824 Fragments arranged by size: 2824 2327 865 451 298 157 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2553 3216 3554 4035 5585 882 Size: 617 1054 663 338 481 1550 2219 Fragments arranged by size: 2219 1550 1054 663 617 481 338 BsaAI yAC'GTr Cuts at: 493 1204 2809 4713 493 Size: 711 1605 1904 2702 Fragments arranged by size: 2702 1904 1605 711 BsaBI GATnn'nnATC Cuts at: 6916 6916 Size: 6922 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4729 5111 5746 275 Size: 53 83 186 936 3196 382 635 1451 Fragments arranged by size: 3196 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2091 5355 5753 6027 6174 6585 2091 Size: 3264 398 274 147 411 2428 Fragments arranged by size: 3264 2428 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2003 2033 2215 2245 2317 Size: 30 165 30 600 30 182 30 72 Cuts at: 2317 2347 2568 2598 2996 3026 3822 3852 1178 Size: 30 221 30 398 30 796 30 4248 Fragments arranged by size: 4248 796 600 398 221 182 165 72 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3068 3557 1186 Size: 1882 489 4551 Fragments arranged by size: 4551 1882 489 BseYI C'CCAG_C Cuts at: 3172 3669 4025 6125 6621 3172 Size: 497 356 2100 496 3473 Fragments arranged by size: 3473 2100 497 496 356 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2375 3055 3059 3230 1163 Size: 573 639 680 4 171 4855 Fragments arranged by size: 4855 680 639 573 171 4 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4372 5133 6161 1540 Size: 275 186 2371 761 1028 2301 Fragments arranged by size: 2371 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2225 2880 3192 4060 4983 5068 6139 2225 Size: 655 312 868 923 85 1071 3008 Fragments arranged by size: 3008 1071 923 868 655 312 85 BsmI GAATG_Cn' Cuts at: 4433 4526 4433 Size: 93 6829 BsmBI CGTCTCn'nnnn_ Cuts at: 4151 4151 Size: 6922 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2225 2482 2880 2979 3192 3856 4060 Size: 399 435 257 398 99 213 664 204 Cuts at: 4060 4241 4983 5068 6139 1391 Size: 181 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 664 435 399 398 257 213 204 181 99 85 BspEI T'CCGG_A Cuts at: 6585 6585 Size: 6922 BspHI T'CATG_A Cuts at: 4811 6541 4811 Size: 1730 5192 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3034 3475 4277 844 Size: 2190 441 802 3489 Fragments arranged by size: 3489 2190 802 441 BsrBI CCG'CTC Cuts at: 1394 1823 4809 1394 Size: 429 2986 3507 Fragments arranged by size: 3507 2986 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2598 3123 4020 5417 5591 65 Size: 1183 1350 525 897 1397 174 1396 Fragments arranged by size: 1397 1396 1350 1183 897 525 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5566 5753 1700 Size: 110 3756 187 2869 Fragments arranged by size: 3756 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2214 96 Size: 2118 4804 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6922 BssSI C'ACGA_G Cuts at: 4982 5994 4982 Size: 1012 5910 BstAPI GCAn_nnn'nTGC Cuts at: 794 2283 3046 3101 3940 794 Size: 1489 763 55 839 3776 Fragments arranged by size: 3776 1489 839 763 55 BstBI TT'CG_AA Cuts at: 5726 5726 Size: 6922 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6922 BstXI CCAn_nnnn'nTGG Cuts at: 2302 3205 2302 Size: 903 6019 BstYI r'GATC_y Cuts at: 1972 2245 2518 2910 3643 4125 4252 4619 5004 Size: 273 273 392 733 482 127 367 385 Cuts at: 5004 5021 6473 6559 6571 6917 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 733 482 392 385 367 346 273 273 127 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2339 2339 Size: 6922 BtgI C'CryG_G Cuts at: 513 1065 3340 4037 513 Size: 552 2275 697 3398 Fragments arranged by size: 3398 2275 697 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3994 487 Size: 683 56 2768 3415 Fragments arranged by size: 3415 2768 683 56 BtsI GCAGTG_nn' Cuts at: 2695 3435 4438 5232 5252 2695 Size: 740 1003 794 20 4365 Fragments arranged by size: 4365 1003 794 740 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6922 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6887 DraI TTT'AAA Cuts at: 4226 4226 Size: 6922 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5929 809 Size: 507 4613 1802 Fragments arranged by size: 4613 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2259 4034 4369 Size: 54 1425 50 275 18 429 1775 335 Cuts at: 4369 5258 6887 8 Size: 889 1629 43 Fragments arranged by size: 1775 1629 1425 889 429 335 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4369 1537 Size: 275 2557 4090 Fragments arranged by size: 4090 2557 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3279 3557 3807 3990 4852 1353 Size: 1926 278 250 183 862 3423 Fragments arranged by size: 3423 1926 862 278 250 183 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5509 5883 6029 387 Size: 1064 138 3920 374 146 1280 Fragments arranged by size: 3920 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2878 2878 Size: 6922 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2063 2487 2673 2847 3273 3831 3870 Size: 393 431 424 186 174 426 558 39 Cuts at: 3870 3959 4985 5582 6369 6637 1239 Size: 89 1026 597 787 268 1524 Fragments arranged by size: 1524 1026 787 597 558 431 426 424 393 268 186 174 89 39 EcoNI CCTnn'n_nnAGG Cuts at: 2410 2410 Size: 6922 EcoO109I rG'GnC_Cy Cuts at: 2840 3268 3797 2840 Size: 428 529 5965 Fragments arranged by size: 5965 529 428 EcoRI G'AATT_C Cuts at: 3351 3956 4231 4609 3351 Size: 605 275 378 5664 Fragments arranged by size: 5664 605 378 275 FspI TGC'GCA Cuts at: 5428 5428 Size: 6922 HaeII r_GCGC'y Cuts at: 1678 6069 1678 Size: 4391 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2409 2434 2441 2466 2568 Size: 32 135 32 837 25 7 25 102 Cuts at: 2568 2600 5563 5595 5637 5669 1373 Size: 32 2963 32 42 32 2626 Fragments arranged by size: 2963 2626 837 135 102 42 32 32 32 32 32 25 25 7 HincII GTy'rAC Cuts at: 669 1657 1938 4266 4512 669 Size: 988 281 2328 246 3079 Fragments arranged by size: 3079 2328 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6922 HpaI GTT'AAC Cuts at: 4512 4512 Size: 6922 KpnI G_GTAC'C Cuts at: 4247 4247 Size: 6922 MfeI C'AATT_G Cuts at: 2523 4203 4521 2523 Size: 1680 318 4924 Fragments arranged by size: 4924 1680 318 MluI A'CGCG_T Cuts at: 6798 6798 Size: 6922 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2703 3381 6036 6220 1305 Size: 1398 678 2655 184 2007 Fragments arranged by size: 2655 2007 1398 678 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2261 10 Size: 54 1425 343 429 4671 Fragments arranged by size: 4671 1425 429 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3077 3203 3692 4013 4880 5239 5398 Size: 648 1911 126 489 321 867 359 159 Cuts at: 5398 518 Size: 2042 Fragments arranged by size: 2042 1911 867 648 489 359 321 159 126 MspA1I CmG'CkG Cuts at: 1606 3416 4338 5015 6163 6408 1606 Size: 1810 922 677 1148 245 2120 Fragments arranged by size: 2120 1810 1148 922 677 245 NcoI C'CATG_G Cuts at: 513 1065 3340 4037 513 Size: 552 2275 697 3398 Fragments arranged by size: 3398 2275 697 552 NdeI CA'TA_TG Cuts at: 387 2227 387 Size: 1840 5082 NheI G'CTAG_C Cuts at: 1052 2848 1052 Size: 1796 5126 NotI GC'GGCC_GC Cuts at: 4369 4369 Size: 6922 NsiI A_TGCA'T Cuts at: 2448 2448 Size: 6922 NspI r_CATG'y Cuts at: 1132 1229 2143 2221 2332 3487 4280 5825 1132 Size: 97 914 78 111 1155 793 1545 2229 Fragments arranged by size: 2229 1545 1155 914 793 111 97 78 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6922 PciI A'CATG_T Cuts at: 2139 2217 5821 2139 Size: 78 3604 3240 Fragments arranged by size: 3604 3240 78 PflMI CCAn_nnn'nTGG Cuts at: 1108 3704 1108 Size: 2596 4326 PmeI GTTT'AAAC Cuts at: 4226 4226 Size: 6922 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4991 5023 6504 6536 4991 Size: 32 1481 32 5377 Fragments arranged by size: 5377 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2840 3797 2840 Size: 957 5965 PshAI GACnn'nnGTC Cuts at: 1613 3546 1613 Size: 1933 4989 PsiI TTA'TAA Cuts at: 4492 4492 Size: 6922 PstI C_TGCA'G Cuts at: 830 4274 830 Size: 3444 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6922 PvuII CAG'CTG Cuts at: 3416 3416 Size: 6922 SacI G_AGCT'C Cuts at: 2880 2880 Size: 6922 SalI G'TCGA_C Cuts at: 1936 4264 1936 Size: 2328 4594 SapI GCTCTTCn'nnn_ Cuts at: 3990 3990 Size: 6922 SbfI CC_TGCA'GG Cuts at: 4274 4274 Size: 6922 ScaI AGT'ACT Cuts at: 5170 6882 5170 Size: 1712 5210 SexAI A'CCwGG_T Cuts at: 2926 3225 2926 Size: 299 6623 SfcI C'TryA_G Cuts at: 826 1045 2181 2993 4270 5405 6086 6277 826 Size: 219 1136 812 1277 1135 681 191 1471 Fragments arranged by size: 1471 1277 1136 1135 812 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6922 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6922 SmaI CCC'GGG Cuts at: 1872 4249 1872 Size: 2377 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2127 2542 2735 5026 5927 6189 Size: 197 926 184 415 193 2291 901 262 Cuts at: 6189 820 Size: 1553 Fragments arranged by size: 2291 1553 926 901 415 262 197 193 184 SnaBI TAC'GTA Cuts at: 493 4713 493 Size: 4220 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6922 SphI G_CATG'C Cuts at: 1132 4280 1132 Size: 3148 3774 SspI AAT'ATT Cuts at: 5 52 4846 5 Size: 47 4794 2081 Fragments arranged by size: 4794 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6922 StyI C'CwwG_G Cuts at: 513 1065 2014 2428 3340 3944 4037 4361 513 Size: 552 949 414 912 604 93 324 3074 Fragments arranged by size: 3074 949 912 604 552 414 324 93 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3558 4949 4966 5119 5304 5749 325 Size: 1255 1978 1391 17 153 185 445 1498 Fragments arranged by size: 1978 1498 1391 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2214 3248 5168 Size: 274 80 33 51 1443 237 1034 1920 Cuts at: 5168 6880 96 Size: 1712 138 Fragments arranged by size: 1920 1712 1443 1034 274 237 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2215 2247 2335 2367 2996 3028 6882 6914 2215 Size: 32 88 32 629 32 3854 32 2223 Fragments arranged by size: 3854 2223 629 88 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6922 XbaI T'CTAG_A Cuts at: 2551 4258 2551 Size: 1707 5215 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6922 XmaI C'CCGG_G Cuts at: 1870 4247 1870 Size: 2377 4545 XmnI GAAnn'nnTTC Cuts at: 3351 3408 5051 3351 Size: 57 1643 5222 Fragments arranged by size: 5222 1643 57 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4730 276 Size: 53 83 186 936 3196 2468 Fragments arranged by size: 3196 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI