(Circular) (Six-base) MAPSORT of: pFN21AB5353.seq Check: 271 from: 1 to: 5878 pFN21AB5353 5878 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3688 278 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3221 1937 Size: 1284 4594 Acc65I G'GTAC_C Cuts at: 3199 3199 Size: 5878 AclI AA'CG_TT Cuts at: 1563 4005 4378 1563 Size: 2442 373 3063 Fragments arranged by size: 3063 2442 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2603 2937 2973 3941 5325 5593 1632 Size: 971 334 36 968 1384 268 1917 Fragments arranged by size: 1917 1384 971 968 334 268 36 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5681 AflIII A'CryG_T Cuts at: 1203 4777 5754 1203 Size: 3574 977 1327 Fragments arranged by size: 3574 1327 977 AgeI A'CCGG_T Cuts at: 4709 4709 Size: 5878 AhdI GACnn_n'nnGTC Cuts at: 4607 4607 Size: 5878 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5878 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5878 AlwNI CAG_nnn'CTG Cuts at: 2621 3621 5193 2621 Size: 1000 1572 3306 Fragments arranged by size: 3306 1572 1000 ApaLI G'TGCA_C Cuts at: 2268 3935 5091 2268 Size: 1667 1156 3055 Fragments arranged by size: 3055 1667 1156 ApoI r'AATT_y Cuts at: 1493 2285 2502 2510 2648 3187 3413 3565 5674 Size: 792 217 8 138 539 226 152 2109 Cuts at: 5674 1493 Size: 1697 Fragments arranged by size: 2109 1697 792 539 226 217 152 138 8 AseI AT'TA_AT Cuts at: 160 1032 2367 4432 160 Size: 872 1335 2065 1606 Fragments arranged by size: 2065 1606 1335 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 AvaI C'yCGr_G Cuts at: 1870 1943 3203 1870 Size: 73 1260 4545 Fragments arranged by size: 4545 1260 73 AvrII C'CTAG_G Cuts at: 2216 2216 Size: 5878 BamHI G'GATC_C Cuts at: 3208 3575 3208 Size: 367 5511 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2775 3199 4654 618 Size: 325 217 627 988 424 1455 1842 Fragments arranged by size: 1842 1455 988 627 424 325 217 BanII G_rGCy'C Cuts at: 1391 3197 1391 Size: 1806 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2496 928 Size: 971 597 4310 Fragments arranged by size: 4310 971 597 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4069 4103 1903 Size: 34 2132 34 3678 Fragments arranged by size: 3678 2132 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2192 3770 4980 2192 Size: 1578 1210 3090 Fragments arranged by size: 3090 1578 1210 BclI T'GATC_A Cuts at: 1548 2041 1548 Size: 493 5385 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4489 136 Size: 107 122 71 631 605 2817 1525 Fragments arranged by size: 2817 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2935 5873 2935 Size: 2938 2940 BlpI GC'TnA_GC Cuts at: 3295 3295 Size: 5878 Bme1580I G_kGCm'C Cuts at: 1790 2272 3939 5095 1790 Size: 482 1667 1156 2573 Fragments arranged by size: 2573 1667 1156 482 BmgBI CAC'GTC Cuts at: 1318 2111 2849 1318 Size: 793 738 4347 Fragments arranged by size: 4347 793 738 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3609 4567 442 Size: 416 53 83 399 24 2192 958 1753 Fragments arranged by size: 2192 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5878 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3054 4538 1239 Size: 1815 1484 2579 Fragments arranged by size: 2579 1815 1484 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4003 4868 5166 4003 Size: 865 298 4715 Fragments arranged by size: 4715 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4541 882 Size: 617 3042 2219 Fragments arranged by size: 3042 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2996 3669 493 Size: 711 1792 673 2702 Fragments arranged by size: 2702 1792 711 673 BsaBI GATnn'nnATC Cuts at: 2730 5872 2730 Size: 3142 2736 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3685 4067 4702 275 Size: 53 83 186 936 2152 382 635 1451 Fragments arranged by size: 2152 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4311 4709 4983 5130 5541 4311 Size: 398 274 147 411 4648 Fragments arranged by size: 4648 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5653 Fragments arranged by size: 5653 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5878 BseYI C'CCAG_C Cuts at: 3157 5081 5577 3157 Size: 1924 496 3458 Fragments arranged by size: 3458 1924 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5305 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3328 4089 5117 1540 Size: 275 186 1327 761 1028 2301 Fragments arranged by size: 2301 1327 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2272 3939 4024 5095 2272 Size: 1667 85 1071 3055 Fragments arranged by size: 3055 1667 1071 85 BsmI GAATG_Cn' Cuts at: 2245 3389 3482 2245 Size: 1144 93 4641 Fragments arranged by size: 4641 1144 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2272 3197 3939 4024 5095 1391 Size: 399 482 925 742 85 1071 2174 Fragments arranged by size: 2174 1071 925 742 482 399 85 BspEI T'CCGG_A Cuts at: 5541 5541 Size: 5878 BspHI T'CATG_A Cuts at: 2302 3767 5497 2302 Size: 1465 1730 2683 Fragments arranged by size: 2683 1730 1465 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2262 3166 3233 844 Size: 1418 904 67 3489 Fragments arranged by size: 3489 1418 904 67 BsrBI CCG'CTC Cuts at: 1394 1823 3765 1394 Size: 429 1942 3507 Fragments arranged by size: 3507 1942 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4373 4547 65 Size: 1183 3125 174 1396 Fragments arranged by size: 3125 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4522 4709 1700 Size: 110 2712 187 2869 Fragments arranged by size: 2869 2712 187 110 BsrGI T'GTAC_A Cuts at: 96 2768 96 Size: 2672 3206 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5878 BssSI C'ACGA_G Cuts at: 3938 4950 3938 Size: 1012 4866 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5878 BstBI TT'CG_AA Cuts at: 2976 4682 2976 Size: 1706 4172 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5878 BstYI r'GATC_y Cuts at: 1972 2935 3208 3575 3960 3977 5429 5515 5527 Size: 963 273 367 385 17 1452 86 12 Cuts at: 5527 5873 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 963 385 367 346 273 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4387 Fragments arranged by size: 4387 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5139 Fragments arranged by size: 5139 683 56 BtsI GCAGTG_nn' Cuts at: 3394 4188 4208 3394 Size: 794 20 5064 Fragments arranged by size: 5064 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5878 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2830 2865 529 Size: 35 2266 35 3542 Fragments arranged by size: 3542 2266 35 35 DraI TTT'AAA Cuts at: 3182 3182 Size: 5878 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2175 4885 809 Size: 507 859 2710 1802 Fragments arranged by size: 2710 1802 859 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2623 3325 4214 Size: 54 1425 50 275 18 793 702 889 Cuts at: 4214 5843 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 793 702 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3325 1537 Size: 275 1513 4090 Fragments arranged by size: 4090 1513 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2070 3808 1353 Size: 717 1738 3423 Fragments arranged by size: 3423 1738 717 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4465 4839 4985 387 Size: 1064 138 2876 374 146 1280 Fragments arranged by size: 2876 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2603 2937 2973 3054 3941 4538 5325 Size: 393 971 334 36 81 887 597 787 Cuts at: 5325 5593 1239 Size: 268 1524 Fragments arranged by size: 1524 971 887 787 597 393 334 268 81 36 EcoRI G'AATT_C Cuts at: 2502 3187 3565 2502 Size: 685 378 4815 Fragments arranged by size: 4815 685 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2186 2978 2186 Size: 792 5086 FspI TGC'GCA Cuts at: 4384 4384 Size: 5878 HaeII r_GCGC'y Cuts at: 1678 5025 1678 Size: 3347 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2122 2154 2920 2952 4519 Size: 32 135 32 550 32 766 32 1567 Cuts at: 4519 4551 4593 4625 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1567 766 550 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2520 2539 3222 3468 669 Size: 988 281 582 19 683 246 3079 Fragments arranged by size: 3079 988 683 582 281 246 19 HindIII A'AGCT_T Cuts at: 748 748 Size: 5878 HpaI GTT'AAC Cuts at: 2520 3468 2520 Size: 948 4930 KpnI G_GTAC'C Cuts at: 3203 3203 Size: 5878 MfeI C'AATT_G Cuts at: 3477 3477 Size: 5878 MluI A'CGCG_T Cuts at: 5754 5754 Size: 5878 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4992 5176 1305 Size: 3687 184 2007 Fragments arranged by size: 3687 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2625 10 Size: 54 1425 343 793 3263 Fragments arranged by size: 3263 1425 793 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3836 4195 4354 518 Size: 648 2670 359 159 2042 Fragments arranged by size: 2670 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3294 3971 5119 5364 1606 Size: 1688 677 1148 245 2120 Fragments arranged by size: 2120 1688 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4387 Fragments arranged by size: 4387 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5878 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5878 NotI GC'GGCC_GC Cuts at: 3325 3325 Size: 5878 NspI r_CATG'y Cuts at: 1132 1229 3236 4781 1132 Size: 97 2007 1545 2229 Fragments arranged by size: 2229 2007 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5878 PciI A'CATG_T Cuts at: 4777 4777 Size: 5878 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5878 PmeI GTTT'AAAC Cuts at: 3182 3182 Size: 5878 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3947 3979 5460 5492 3947 Size: 32 1481 32 4333 Fragments arranged by size: 4333 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5878 PsiI TTA'TAA Cuts at: 3448 3448 Size: 5878 PstI C_TGCA'G Cuts at: 830 2971 3230 830 Size: 2141 259 3478 Fragments arranged by size: 3478 2141 259 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5878 SalI G'TCGA_C Cuts at: 1936 3220 1936 Size: 1284 4594 SbfI CC_TGCA'GG Cuts at: 3230 3230 Size: 5878 ScaI AGT'ACT Cuts at: 4126 5838 4126 Size: 1712 4166 SfcI C'TryA_G Cuts at: 826 1045 2967 3226 4361 5042 5233 826 Size: 219 1922 259 1135 681 191 1471 Fragments arranged by size: 1922 1471 1135 681 259 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5878 SmaI CCC'GGG Cuts at: 1872 3205 1872 Size: 1333 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3982 4883 5145 820 Size: 197 926 2039 901 262 1553 Fragments arranged by size: 2039 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3669 493 Size: 3176 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5878 SphI G_CATG'C Cuts at: 1132 3236 1132 Size: 2104 3774 SspI AAT'ATT Cuts at: 5 52 2672 3802 5 Size: 47 2620 1130 2081 Fragments arranged by size: 2620 2081 1130 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5878 StyI C'CwwG_G Cuts at: 513 1065 2004 2216 3317 513 Size: 552 939 212 1101 3074 Fragments arranged by size: 3074 1101 939 552 212 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3905 3922 4075 4260 4705 325 Size: 1255 2325 17 153 185 445 1498 Fragments arranged by size: 2325 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2768 4124 5836 Size: 274 80 33 51 1443 791 1356 1712 Cuts at: 5836 96 Size: 138 Fragments arranged by size: 1712 1443 1356 791 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5838 5870 5838 Size: 32 5846 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5878 XbaI T'CTAG_A Cuts at: 3214 3214 Size: 5878 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5878 XmaI C'CCGG_G Cuts at: 1870 3203 1870 Size: 1333 4545 XmnI GAAnn'nnTTC Cuts at: 2577 4007 2577 Size: 1430 4448 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3686 276 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI