(Circular) (Six-base) MAPSORT of: pFN21AB5343.seq Check: 7429 from: 1 to: 6469 pFN21AB5343 6469 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3162 3162 Size: 6469 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3108 4279 278 Size: 53 83 186 936 1572 1171 2468 Fragments arranged by size: 2468 1572 1171 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3138 3812 1937 Size: 1201 674 4594 Fragments arranged by size: 4594 1201 674 Acc65I G'GTAC_C Cuts at: 3790 3790 Size: 6469 AclI AA'CG_TT Cuts at: 1563 2192 4596 4969 1563 Size: 629 2404 373 3063 Fragments arranged by size: 3063 2404 629 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2144 3014 3344 4532 5916 6184 1632 Size: 512 870 330 1188 1384 268 1917 Fragments arranged by size: 1917 1384 1188 870 512 330 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6272 AflIII A'CryG_T Cuts at: 1203 2799 5368 6345 1203 Size: 1596 2569 977 1327 Fragments arranged by size: 2569 1596 1327 977 AgeI A'CCGG_T Cuts at: 5300 5300 Size: 6469 AhdI GACnn_n'nnGTC Cuts at: 5198 5198 Size: 6469 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6469 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6469 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3291 3323 3291 Size: 32 6437 AlwNI CAG_nnn'CTG Cuts at: 3461 3574 4212 5784 3461 Size: 113 638 1572 4146 Fragments arranged by size: 4146 1572 638 113 ApaLI G'TGCA_C Cuts at: 4526 5682 4526 Size: 1156 5313 ApoI r'AATT_y Cuts at: 1493 3778 4004 4156 6265 1493 Size: 2285 226 152 2109 1697 Fragments arranged by size: 2285 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5023 160 Size: 872 3991 1606 Fragments arranged by size: 3991 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6469 AvaI C'yCGr_G Cuts at: 1870 1943 2753 3794 1870 Size: 73 810 1041 4545 Fragments arranged by size: 4545 1041 810 73 BamHI G'GATC_C Cuts at: 3799 4166 3799 Size: 367 6102 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2792 2866 3016 3545 3790 Size: 325 217 627 1005 74 150 529 245 Cuts at: 3790 5245 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1005 627 529 325 245 217 150 74 BanII G_rGCy'C Cuts at: 1391 3788 1391 Size: 2397 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2539 2943 928 Size: 971 640 404 4454 Fragments arranged by size: 4454 971 640 404 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4660 4694 1903 Size: 34 2723 34 3678 Fragments arranged by size: 3678 2723 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2385 4361 5571 2385 Size: 1976 1210 3283 Fragments arranged by size: 3283 1976 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6469 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2852 3491 3755 Size: 107 122 71 631 605 1180 639 264 Cuts at: 3755 5080 136 Size: 1325 1525 Fragments arranged by size: 1525 1325 1180 639 631 605 264 122 107 71 BglII A'GATC_T Cuts at: 6464 6464 Size: 6469 BlpI GC'TnA_GC Cuts at: 2090 3886 2090 Size: 1796 4673 Bme1580I G_kGCm'C Cuts at: 1790 2536 2795 3021 4530 5686 1790 Size: 746 259 226 1509 1156 2573 Fragments arranged by size: 2573 1509 1156 746 259 226 BmgBI CAC'GTC Cuts at: 1318 2647 1318 Size: 1329 5140 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2597 3184 4200 Size: 416 53 83 399 24 1180 587 1016 Cuts at: 4200 5158 442 Size: 958 1753 Fragments arranged by size: 1753 1180 1016 958 587 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6469 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3262 3262 Size: 6469 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2069 2975 3578 5129 1239 Size: 830 906 603 1551 2579 Fragments arranged by size: 2579 1551 906 830 603 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2036 4594 5459 5757 2036 Size: 2558 865 298 2748 Fragments arranged by size: 2748 2558 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5132 882 Size: 617 3633 2219 Fragments arranged by size: 3633 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2467 3181 4260 493 Size: 711 1263 714 1079 2702 Fragments arranged by size: 2702 1263 1079 714 711 BsaBI GATnn'nnATC Cuts at: 6463 6463 Size: 6469 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3105 4276 4658 5293 Size: 53 83 186 936 1572 1171 382 635 Cuts at: 5293 275 Size: 1451 Fragments arranged by size: 1572 1451 1171 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2271 2280 4902 5300 5574 5721 6132 2271 Size: 9 2622 398 274 147 411 2608 Fragments arranged by size: 2622 2608 411 398 274 147 9 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3040 3070 3291 3321 1178 Size: 30 165 30 1637 30 221 30 4326 Fragments arranged by size: 4326 1637 221 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2213 2894 2897 3395 3398 3452 3455 3458 Size: 1027 681 3 498 3 54 3 3 Cuts at: 3458 3599 3602 1186 Size: 141 3 4053 Fragments arranged by size: 4053 1027 681 498 141 54 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2695 5672 6168 2695 Size: 2977 496 2996 Fragments arranged by size: 2996 2977 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2489 3203 3275 1163 Size: 573 753 714 72 4357 Fragments arranged by size: 4357 753 714 573 72 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3142 3919 4680 5708 1540 Size: 275 186 1141 777 761 1028 2301 Fragments arranged by size: 2301 1141 1028 777 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4530 4615 5686 4530 Size: 85 1071 5313 Fragments arranged by size: 5313 1071 85 BsmI GAATG_Cn' Cuts at: 2118 3980 4073 2118 Size: 1862 93 4514 Fragments arranged by size: 4514 1862 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2536 2795 3021 3788 4530 4615 5686 Size: 399 746 259 226 767 742 85 1071 Cuts at: 5686 1391 Size: 2174 Fragments arranged by size: 2174 1071 767 746 742 399 259 226 85 BspEI T'CCGG_A Cuts at: 2271 2280 6132 2271 Size: 9 3852 2608 Fragments arranged by size: 3852 2608 9 BspHI T'CATG_A Cuts at: 4358 6088 4358 Size: 1730 4739 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3162 3824 844 Size: 2318 662 3489 Fragments arranged by size: 3489 2318 662 BsrBI CCG'CTC Cuts at: 1394 1823 4356 1394 Size: 429 2533 3507 Fragments arranged by size: 3507 2533 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4964 5138 65 Size: 1183 3716 174 1396 Fragments arranged by size: 3716 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2490 2783 3141 5113 5300 1700 Size: 110 680 293 358 1972 187 2869 Fragments arranged by size: 2869 1972 680 358 293 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6469 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6469 BssSI C'ACGA_G Cuts at: 4529 5541 4529 Size: 1012 5457 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6469 BstBI TT'CG_AA Cuts at: 5273 5273 Size: 6469 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6469 BstXI CCAn_nnnn'nTGG Cuts at: 2695 3538 3607 3697 2695 Size: 843 69 90 5467 Fragments arranged by size: 5467 843 90 69 BstYI r'GATC_y Cuts at: 1972 3799 4166 4551 4568 6020 6106 6118 6464 Size: 1827 367 385 17 1452 86 12 346 Cuts at: 6464 1972 Size: 1977 Fragments arranged by size: 1977 1827 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2447 3231 513 Size: 552 939 443 784 3751 Fragments arranged by size: 3751 939 784 552 443 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5730 Fragments arranged by size: 5730 683 56 BtsI GCAGTG_nn' Cuts at: 3985 4779 4799 3985 Size: 794 20 5655 Fragments arranged by size: 5655 794 20 ClaI AT'CG_AT Cuts at: 1554 2388 1554 Size: 834 5635 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6434 DraI TTT'AAA Cuts at: 2325 3773 2325 Size: 1448 5021 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2657 5476 809 Size: 507 1341 2819 1802 Fragments arranged by size: 2819 1802 1341 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2664 3609 Size: 54 1425 50 275 18 213 621 945 Cuts at: 3609 3916 4805 6434 8 Size: 307 889 1629 43 Fragments arranged by size: 1629 1425 945 889 621 307 275 213 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3916 1537 Size: 275 2104 4090 Fragments arranged by size: 4090 2104 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2291 3372 3384 3417 3474 3498 4399 1353 Size: 938 1081 12 33 57 24 901 3423 Fragments arranged by size: 3423 1081 938 901 57 33 24 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2702 3716 5056 5430 5576 387 Size: 1064 138 1113 1014 1340 374 146 1280 Fragments arranged by size: 1340 1280 1113 1064 1014 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2144 2975 3014 3344 3578 4532 Size: 393 437 75 831 39 330 234 954 Cuts at: 4532 5129 5916 6184 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 954 831 787 597 437 393 330 268 234 75 39 EcoNI CCTnn'n_nnAGG Cuts at: 2540 2540 Size: 6469 EcoO109I rG'GnC_Cy Cuts at: 2401 2989 3028 3352 2401 Size: 588 39 324 5518 Fragments arranged by size: 5518 588 324 39 EcoRI G'AATT_C Cuts at: 3778 4156 3778 Size: 378 6091 FspI TGC'GCA Cuts at: 2540 4975 2540 Size: 2435 4034 HaeII r_GCGC'y Cuts at: 1678 3607 5616 1678 Size: 1929 2009 2531 Fragments arranged by size: 2531 2009 1929 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2402 2434 5110 5142 5184 Size: 32 135 32 830 32 2676 32 42 Cuts at: 5184 5216 1373 Size: 32 2626 Fragments arranged by size: 2676 2626 830 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2167 2242 2707 3139 3813 4059 Size: 988 281 229 75 465 432 674 246 Cuts at: 4059 669 Size: 3079 Fragments arranged by size: 3079 988 674 465 432 281 246 229 75 HindIII A'AGCT_T Cuts at: 748 2978 748 Size: 2230 4239 HpaI GTT'AAC Cuts at: 2167 4059 2167 Size: 1892 4577 KpnI G_GTAC'C Cuts at: 3794 3794 Size: 6469 MfeI C'AATT_G Cuts at: 4068 4068 Size: 6469 MluI A'CGCG_T Cuts at: 2799 6345 2799 Size: 3546 2923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2784 5583 5767 1305 Size: 1479 2799 184 2007 Fragments arranged by size: 2799 2007 1479 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2666 3611 10 Size: 54 1425 343 834 945 2868 Fragments arranged by size: 2868 1425 945 834 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3617 4427 4786 4945 518 Size: 648 2451 810 359 159 2042 Fragments arranged by size: 2451 2042 810 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2089 2449 2482 3259 3343 3571 3885 4562 Size: 483 360 33 777 84 228 314 677 Cuts at: 4562 5710 5955 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 777 677 483 360 314 245 228 84 33 NaeI GCC'GGC Cuts at: 2492 2492 Size: 6469 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4978 Fragments arranged by size: 4978 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6469 NgoMIV G'CCGG_C Cuts at: 2490 2490 Size: 6469 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6469 NotI GC'GGCC_GC Cuts at: 3916 3916 Size: 6469 NspI r_CATG'y Cuts at: 1132 1229 3827 5372 1132 Size: 97 2598 1545 2229 Fragments arranged by size: 2598 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3531 1348 Size: 2183 4286 PciI A'CATG_T Cuts at: 5368 5368 Size: 6469 PflMI CCAn_nnn'nTGG Cuts at: 1108 2604 1108 Size: 1496 4973 PmeI GTTT'AAAC Cuts at: 3773 3773 Size: 6469 PmlI CAC'GTG Cuts at: 2467 2467 Size: 6469 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3291 3323 4538 4570 6051 6083 3291 Size: 32 1215 32 1481 32 3677 Fragments arranged by size: 3677 1481 1215 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2401 3352 2401 Size: 951 5518 PshAI GACnn'nnGTC Cuts at: 1613 3161 1613 Size: 1548 4921 PsiI TTA'TAA Cuts at: 4039 4039 Size: 6469 PspXI vC'TCGA_Gb Cuts at: 2753 2753 Size: 6469 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2366 2398 2366 Size: 32 6437 PstI C_TGCA'G Cuts at: 830 3342 3821 830 Size: 2512 479 3478 Fragments arranged by size: 3478 2512 479 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6469 PvuII CAG'CTG Cuts at: 2089 2482 3259 2089 Size: 393 777 5299 Fragments arranged by size: 5299 777 393 SacII CC_GC'GG Cuts at: 2450 2450 Size: 6469 SalI G'TCGA_C Cuts at: 1936 3137 3811 1936 Size: 1201 674 4594 Fragments arranged by size: 4594 1201 674 SapI GCTCTTCn'nnn_ Cuts at: 2291 3498 2291 Size: 1207 5262 SbfI CC_TGCA'GG Cuts at: 3821 3821 Size: 6469 ScaI AGT'ACT Cuts at: 3242 4717 6429 3242 Size: 1475 1712 3282 Fragments arranged by size: 3282 1712 1475 SfcI C'TryA_G Cuts at: 826 1045 2178 2313 2653 3338 3817 4952 5633 Size: 219 1133 135 340 685 479 1135 681 Cuts at: 5633 5824 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1133 685 681 479 340 219 191 135 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6469 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6469 SmaI CCC'GGG Cuts at: 1872 3796 1872 Size: 1924 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2015 2753 4573 5474 5736 820 Size: 197 926 72 738 1820 901 262 1553 Fragments arranged by size: 1820 1553 926 901 738 262 197 72 SnaBI TAC'GTA Cuts at: 493 4260 493 Size: 3767 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6469 SphI G_CATG'C Cuts at: 1132 3827 1132 Size: 2695 3774 SspI AAT'ATT Cuts at: 5 52 4393 5 Size: 47 4341 2081 Fragments arranged by size: 4341 2081 47 StuI AGG'CCT Cuts at: 1517 2013 1517 Size: 496 5973 StyI C'CwwG_G Cuts at: 513 1065 2004 3908 513 Size: 552 939 1904 3074 Fragments arranged by size: 3074 1904 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3140 3184 3302 4496 4513 4666 4851 Size: 1255 1560 44 118 1194 17 153 185 Cuts at: 4851 5296 325 Size: 445 1498 Fragments arranged by size: 1560 1498 1255 1194 445 185 153 118 44 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3240 4715 6427 Size: 274 80 33 51 1443 1263 1475 1712 Cuts at: 6427 96 Size: 138 Fragments arranged by size: 1712 1475 1443 1263 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3040 3072 6429 6461 3040 Size: 32 3357 32 3048 Fragments arranged by size: 3357 3048 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6469 XbaI T'CTAG_A Cuts at: 3805 3805 Size: 6469 XhoI C'TCGA_G Cuts at: 1943 2753 1943 Size: 810 5659 XmaI C'CCGG_G Cuts at: 1870 3794 1870 Size: 1924 4545 XmnI GAAnn'nnTTC Cuts at: 2999 4598 2999 Size: 1599 4870 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3106 4277 276 Size: 53 83 186 936 1572 1171 2468 Fragments arranged by size: 2468 1572 1171 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspOMI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI