(Circular) (Six-base) MAPSORT of: pFN21AB5331.seq Check: 9709 from: 1 to: 5950 pFN21AB5331 5950 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3075 3760 278 Size: 53 83 186 936 1539 685 2468 Fragments arranged by size: 2468 1539 936 685 186 83 53 AccI GT'mk_AC Cuts at: 1937 3090 3293 1937 Size: 1153 203 4594 Fragments arranged by size: 4594 1153 203 Acc65I G'GTAC_C Cuts at: 3271 3271 Size: 5950 AclI AA'CG_TT Cuts at: 1563 4077 4450 1563 Size: 2514 373 3063 Fragments arranged by size: 3063 2514 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2819 4013 5397 5665 1632 Size: 1187 1194 1384 268 1917 Fragments arranged by size: 1917 1384 1194 1187 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5753 AflIII A'CryG_T Cuts at: 1203 2571 2712 4849 5826 1203 Size: 1368 141 2137 977 1327 Fragments arranged by size: 2137 1368 1327 977 141 AgeI A'CCGG_T Cuts at: 2216 4781 2216 Size: 2565 3385 AhdI GACnn_n'nnGTC Cuts at: 4679 4679 Size: 5950 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5950 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2103 2963 1184 Size: 919 860 4171 Fragments arranged by size: 4171 919 860 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2981 3013 2981 Size: 32 5918 AlwNI CAG_nnn'CTG Cuts at: 2098 3693 5265 2098 Size: 1595 1572 2783 Fragments arranged by size: 2783 1595 1572 ApaI G_GGCC'C Cuts at: 2128 3219 2128 Size: 1091 4859 ApaLI G'TGCA_C Cuts at: 4007 5163 4007 Size: 1156 4794 ApoI r'AATT_y Cuts at: 1493 3259 3485 3637 5746 1493 Size: 1766 226 152 2109 1697 Fragments arranged by size: 2109 1766 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4504 160 Size: 872 3472 1606 Fragments arranged by size: 3472 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 AvaI C'yCGr_G Cuts at: 1870 1943 3275 1870 Size: 73 1332 4545 Fragments arranged by size: 4545 1332 73 AvrII C'CTAG_G Cuts at: 2842 2842 Size: 5950 BamHI G'GATC_C Cuts at: 3280 3647 3280 Size: 367 5583 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3271 4726 618 Size: 325 217 627 1484 1455 1842 Fragments arranged by size: 1842 1484 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2128 2601 2853 3219 3269 1391 Size: 737 473 252 366 50 4072 Fragments arranged by size: 4072 737 473 366 252 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3067 3174 928 Size: 971 1168 107 3704 Fragments arranged by size: 3704 1168 971 107 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4141 4175 1903 Size: 34 2204 34 3678 Fragments arranged by size: 3678 2204 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3190 3842 5052 3190 Size: 652 1210 4088 Fragments arranged by size: 4088 1210 652 BclI T'GATC_A Cuts at: 1548 1548 Size: 5950 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4561 136 Size: 107 122 71 631 605 2889 1525 Fragments arranged by size: 2889 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5945 5945 Size: 5950 BlpI GC'TnA_GC Cuts at: 2484 3367 2484 Size: 883 5067 Bme1580I G_kGCm'C Cuts at: 1790 2128 3219 4011 5167 1790 Size: 338 1091 792 1156 2573 Fragments arranged by size: 2573 1156 1091 792 338 BmgBI CAC'GTC Cuts at: 1318 2572 2770 2983 1318 Size: 1254 198 213 4285 Fragments arranged by size: 4285 1254 213 198 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2122 3681 4639 Size: 416 53 83 399 24 705 1559 958 Cuts at: 4639 442 Size: 1753 Fragments arranged by size: 1753 1559 958 705 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5950 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2492 2972 4610 1239 Size: 1253 480 1638 2579 Fragments arranged by size: 2579 1638 1253 480 Bpu10I CC'TnA_GC Cuts at: 2420 2420 Size: 5950 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4075 4940 5238 4075 Size: 865 298 4787 Fragments arranged by size: 4787 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4613 882 Size: 617 3114 2219 Fragments arranged by size: 3114 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2715 3741 493 Size: 711 1511 1026 2702 Fragments arranged by size: 2702 1511 1026 711 BsaBI GATnn'nnATC Cuts at: 2105 5944 2105 Size: 3839 2111 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3072 3757 4139 4774 Size: 53 83 186 936 1539 685 382 635 Cuts at: 4774 275 Size: 1451 Fragments arranged by size: 1539 1451 936 685 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2216 4383 4781 5055 5202 5613 2216 Size: 2167 398 274 147 411 2553 Fragments arranged by size: 2553 2167 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2561 2591 1178 Size: 30 165 30 1158 30 4537 Fragments arranged by size: 4537 1158 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2133 2480 3146 3164 3167 3170 3173 3176 Size: 947 347 666 18 3 3 3 3 Cuts at: 3176 1186 Size: 3960 Fragments arranged by size: 3960 947 666 347 18 3 3 3 3 BseYI C'CCAG_C Cuts at: 2173 2601 3179 3212 5153 5649 2173 Size: 428 578 33 1941 496 2474 Fragments arranged by size: 2474 1941 578 496 428 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5377 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3400 4161 5189 1540 Size: 275 186 1399 761 1028 2301 Fragments arranged by size: 2301 1399 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2870 4011 4096 5167 2870 Size: 1141 85 1071 3653 Fragments arranged by size: 3653 1141 1071 85 BsmI GAATG_Cn' Cuts at: 2667 3461 3554 2667 Size: 794 93 5063 Fragments arranged by size: 5063 794 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2128 2601 2853 2870 3219 3269 4011 Size: 399 338 473 252 17 349 50 742 Cuts at: 4011 4096 5167 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 473 399 349 338 252 85 50 17 BspEI T'CCGG_A Cuts at: 5613 5613 Size: 5950 BspHI T'CATG_A Cuts at: 3839 5569 3839 Size: 1730 4220 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2220 3305 844 Size: 1376 1085 3489 Fragments arranged by size: 3489 1376 1085 BsrBI CCG'CTC Cuts at: 1394 1823 2476 3837 1394 Size: 429 653 1361 3507 Fragments arranged by size: 3507 1361 653 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2446 2835 4445 4619 65 Size: 1183 1198 389 1610 174 1396 Fragments arranged by size: 1610 1396 1198 1183 389 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2216 2373 2870 4594 4781 1700 Size: 110 406 157 497 1724 187 2869 Fragments arranged by size: 2869 1724 497 406 187 157 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5950 BssHII G'CGCG_C Cuts at: 1925 2915 2917 1925 Size: 990 2 4958 Fragments arranged by size: 4958 990 2 BssSI C'ACGA_G Cuts at: 3014 3201 4010 5022 3014 Size: 187 809 1012 3942 Fragments arranged by size: 3942 1012 809 187 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5950 BstBI TT'CG_AA Cuts at: 4754 4754 Size: 5950 BstEII G'GTnAC_C Cuts at: 1184 2071 2293 1184 Size: 887 222 4841 Fragments arranged by size: 4841 887 222 BstYI r'GATC_y Cuts at: 1972 2704 3280 3647 4032 4049 5501 5587 5599 Size: 732 576 367 385 17 1452 86 12 Cuts at: 5599 5945 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 732 576 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5398 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2220 487 Size: 683 56 994 4217 Fragments arranged by size: 4217 994 683 56 BtsI GCAGTG_nn' Cuts at: 3060 3466 4260 4280 3060 Size: 406 794 20 4730 Fragments arranged by size: 4730 794 406 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5950 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5915 DraI TTT'AAA Cuts at: 3254 3254 Size: 5950 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4957 809 Size: 507 3641 1802 Fragments arranged by size: 3641 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3397 4286 5915 Size: 54 1425 50 275 18 1567 889 1629 Cuts at: 5915 8 Size: 43 Fragments arranged by size: 1629 1567 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3397 1537 Size: 275 1585 4090 Fragments arranged by size: 4090 1585 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2028 3880 1353 Size: 675 1852 3423 Fragments arranged by size: 3423 1852 675 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2336 4537 4911 5057 387 Size: 1064 138 747 2201 374 146 1280 Fragments arranged by size: 2201 1280 1064 747 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2492 2819 2972 4013 4610 5397 5665 Size: 393 860 327 153 1041 597 787 268 Cuts at: 5665 1239 Size: 1524 Fragments arranged by size: 1524 1041 860 787 597 393 327 268 153 EcoNI CCTnn'n_nnAGG Cuts at: 2081 3125 2081 Size: 1044 4906 EcoO109I rG'GnC_Cy Cuts at: 2124 3216 2124 Size: 1092 4858 EcoRI G'AATT_C Cuts at: 3259 3637 3259 Size: 378 5572 FspI TGC'GCA Cuts at: 2939 4456 2939 Size: 1517 4433 HaeII r_GCGC'y Cuts at: 1678 3013 5097 1678 Size: 1335 2084 2531 Fragments arranged by size: 2531 2084 1335 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2561 2593 2771 2803 4591 Size: 32 135 32 989 32 178 32 1788 Cuts at: 4591 4623 4665 4697 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1788 989 178 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3294 3540 669 Size: 988 281 1356 246 3079 Fragments arranged by size: 3079 1356 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5950 HpaI GTT'AAC Cuts at: 3540 3540 Size: 5950 KpnI G_GTAC'C Cuts at: 3275 3275 Size: 5950 MfeI C'AATT_G Cuts at: 3549 3549 Size: 5950 MluI A'CGCG_T Cuts at: 5826 5826 Size: 5950 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5064 5248 1305 Size: 3759 184 2007 Fragments arranged by size: 3759 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4128 Fragments arranged by size: 4128 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3908 4267 4426 518 Size: 648 2742 359 159 2042 Fragments arranged by size: 2742 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2201 2483 2609 2832 3004 3179 3366 4043 Size: 595 282 126 223 172 175 187 677 Cuts at: 4043 5191 5436 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 595 282 245 223 187 175 172 126 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5398 NdeI CA'TA_TG Cuts at: 387 387 Size: 5950 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5950 NotI GC'GGCC_GC Cuts at: 3397 3397 Size: 5950 NspI r_CATG'y Cuts at: 1132 1229 2341 3308 4853 1132 Size: 97 1112 967 1545 2229 Fragments arranged by size: 2229 1545 1112 967 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5950 PciI A'CATG_T Cuts at: 4849 4849 Size: 5950 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5950 PfoI T'CCnGG_A Cuts at: 2733 2985 2733 Size: 252 5698 PmeI GTTT'AAAC Cuts at: 3254 3254 Size: 5950 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4019 4051 5532 5564 4019 Size: 32 1481 32 4405 Fragments arranged by size: 4405 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5950 PsiI TTA'TAA Cuts at: 3520 3520 Size: 5950 PspOMI G'GGCC_C Cuts at: 2124 3215 2124 Size: 1091 4859 PstI C_TGCA'G Cuts at: 830 2082 2319 3009 3302 830 Size: 1252 237 690 293 3478 Fragments arranged by size: 3478 1252 690 293 237 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5950 PvuII CAG'CTG Cuts at: 2201 2609 2832 3004 3179 2201 Size: 408 223 172 175 4972 Fragments arranged by size: 4972 408 223 175 172 RsrII CG'GwC_CG Cuts at: 2478 2478 Size: 5950 SalI G'TCGA_C Cuts at: 1936 3292 1936 Size: 1356 4594 SbfI CC_TGCA'GG Cuts at: 3302 3302 Size: 5950 ScaI AGT'ACT Cuts at: 2132 3035 4198 5910 2132 Size: 903 1163 1712 2172 Fragments arranged by size: 2172 1712 1163 903 SfcI C'TryA_G Cuts at: 826 1045 2078 2315 3005 3298 4433 5114 5305 Size: 219 1033 237 690 293 1135 681 191 Cuts at: 5305 826 Size: 1471 Fragments arranged by size: 1471 1135 1033 690 681 293 237 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 SgrAI Cr'CCGG_yG Cuts at: 1700 2216 1700 Size: 516 5434 SmaI CCC'GGG Cuts at: 1872 3277 1872 Size: 1405 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4054 4955 5217 820 Size: 197 926 2111 901 262 1553 Fragments arranged by size: 2111 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3741 493 Size: 3248 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5950 SphI G_CATG'C Cuts at: 1132 3308 1132 Size: 2176 3774 SspI AAT'ATT Cuts at: 5 52 3874 5 Size: 47 3822 2081 Fragments arranged by size: 3822 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5950 StyI C'CwwG_G Cuts at: 513 1065 2181 2842 3219 3389 513 Size: 552 1116 661 377 170 3074 Fragments arranged by size: 3074 1116 661 552 377 170 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3977 3994 4147 4332 4777 325 Size: 1255 2397 17 153 185 445 1498 Fragments arranged by size: 2397 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2130 3033 4196 Size: 274 80 33 51 1443 153 903 1163 Cuts at: 4196 5908 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1163 903 274 153 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5910 5942 5910 Size: 32 5918 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5950 XbaI T'CTAG_A Cuts at: 3286 3286 Size: 5950 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5950 XmaI C'CCGG_G Cuts at: 1870 3275 1870 Size: 1405 4545 XmnI GAAnn'nnTTC Cuts at: 4079 4079 Size: 5950 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3073 3758 276 Size: 53 83 186 936 1539 685 2468 Fragments arranged by size: 2468 1539 936 685 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI