Restriction map for
pFN21AB5285
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI AhdI Alw44I ApaI ApaLI
AscI AseI AsnI AspEI AspHI
AviII AvrII BamHI BbeI BcgI
BciVI BfrI BlnI BsaBI BsaWI
BseAI BsiEI BsiHKAI BsiWI BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HindIII HpaI KasI KspI MamI
MluI MroI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 1258 GGTACC G^GTACC
AcyI 80 GACGCC G[GA]^CG[CT]C
AocI 924 CCTGAGG CC^T[ACGT]AGG
Asp718I 1258 GGTACC G^GTACC
AspI 1327 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2152 CCCGAG C^[CT]CG[GA]G
BalI 1301 TGGCCA TGG^CCA
BanI 1258 GGTACC G^G[CT][GA]CC
BanII 2083 GAGCCC G[GA]GC[CT]^C
BbrPI 819 CACGTG CAC^GTG
BclI 1529 TGATCA T^GATCA
BglI 1736 GCCCAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1851 AGATCT A^GATCT
BlpI 2080 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2080 GCTGAGC GC^T[ACGT]AGC
BsaAI 819 CACGTG [CT]AC^GT[GA]
BsaHI 80 GACGCC G[GA]^CG[CT]C
BsmBI 78 GAGACG CGTCTC(1/5)
BsoBI 2152 CCCGAG C^[CT]CG[GA]G
Bsp1407I 171 TGTACA T^GTACA
BspHI 1428 TCATGA T^CATGA
BsrGI 171 TGTACA T^GTACA
BssSI 1039 CACGAG CACGAG(-5/-1)
Bsu36I 924 CCTGAGG CC^T[ACGT]AGG
CelII 2080 GCTGAGC GC^T[ACGT]AGC
DraII 434 AGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 1301 TGGCCA [CT]^GGCC[GA]
Eco81I 924 CCTGAGG CC^T[ACGT]AGG
EcoO109I 434 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 1706 CCATGG C^C[AT][AT]GG
FbaI 1529 TGATCA T^GATCA
Hin1I 80 GACGCC G[GA]^CG[CT]C
HincII 160 GTCAAC GT[CT]^[GA]AC
HindII 160 GTCAAC GT[CT]^[GA]AC
KpnI 1258 GGTACC GGTAC^C
MfeI 2039 CAATTG C^AATTG
MluNI 1301 TGGCCA TGG^CCA
MscI 1301 TGGCCA TGG^CCA
MunI 2039 CAATTG C^AATTG
NcoI 1706 CCATGG C^CATGG
PflFI 1327 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 34 GAGTC GAGTC(4/5)
PmaCI 819 CACGTG CAC^GTG
PmlI 819 CACGTG CAC^GTG
PstI 1572 CTGCAG CTGCA^G
RcaI 1428 TCATGA T^CATGA
SfcI 1572 CTGCAG C^T[GA][CT]AG
SspBI 171 TGTACA T^GTACA
StyI 1706 CCATGG C^C[AT][AT]GG
Tth111I 1327 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 673 GTCTAC GT^[AC][GT]AC
1511 GTAGAC
AcsI 837 AAATTT [GA]^AATT[CT]
1306 AAATTT
ApoI 837 AAATTT [GA]^AATT[CT]
1306 AAATTT
BbvCI 1912 CCTCAGC CCTCAGC(-5/-2)
2163 CCTCAGC
BcnI 84 CCGGG CC^[GC]GG
1553 CCGGG
BfaI 532 CTAG C^TAG
566 CTAG
BpmI 248 CTCCAG CTGGAG(16/14)
1121 CTGGAG
BsaI 873 GAGACC GGTCTC(1/5)
2141 GAGACC
BsgI 1140 GTGCAG GTGCAG(16/14)
1149 GTGCAG
BsmI 904 GCATTC GAATGC(1/-1)
1377 GCATTC
BstXI 129 CCAGACGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
888 CCATCTCTGTGG
BstYI 561 AGATCC [GA]^GATC[CT]
1851 AGATCT
DsaI 826 CCACGG C^C[GA][CT]GG
1706 CCATGG
EarI 1776 GAAGAG CTCTTC(1/4)
2101 GAAGAG
FauI 1939 CCCGC CCCGC(4/6)
2086 CCCGC
HgaI 80 GACGC GACGC(5/10)
1128 GCGTC
Ksp632I 1776 GAAGAG CTCTTC(1/4)
2101 GAAGAG
MaeI 532 CTAG C^TAG
566 CTAG
MflI 561 AGATCC [GA]^GATC[CT]
1851 AGATCT
MspA1I 193 CAGCTG C[AC]G^C[GT]G
2117 CAGCTG
NciI 84 CCGGG CC^[GC]GG
1553 CCGGG
NspI 1616 ACATGC [GA]CATG^[CT]
1716 ACATGC
PvuII 193 CAGCTG CAG^CTG
2117 CAGCTG
TaqI 17 TCGA T^CGA
1613 TCGA
TfiI 930 GAATC G^A[AT]TC
1822 GATTC
TthHB8I 17 TCGA T^CGA
1613 TCGA
XcmI 512 CCAATGGAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
998 CCAGTGGAGAATTGG
XhoII 561 AGATCC [GA]^GATC[CT]
1851 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI Aor51HI Asp700I AvaII BbsI
BbvI BmyI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
Eco47III EcoRII Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI Tru9I TseI Tsp45I Tsp509I
TspRI XmnI