(Circular) (Six-base) MAPSORT of: pFN21AB5285.seq Check: 8008 from: 1 to: 6886 pFN21AB5285 6886 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4696 278 Size: 53 83 186 936 3160 2468 Fragments arranged by size: 3160 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2679 3517 4229 1937 Size: 742 838 712 4594 Fragments arranged by size: 4594 838 742 712 Acc65I G'GTAC_C Cuts at: 3263 4207 3263 Size: 944 5942 AclI AA'CG_TT Cuts at: 1563 5013 5386 1563 Size: 3450 373 3063 Fragments arranged by size: 3450 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2201 2250 2327 2376 2390 2508 4949 6333 Size: 569 49 77 49 14 118 2441 1384 Cuts at: 6333 6601 1632 Size: 268 1917 Fragments arranged by size: 2441 1917 1384 569 268 118 77 49 49 14 AfeI AGC'GCT Cuts at: 3440 3485 3635 3440 Size: 45 150 6691 Fragments arranged by size: 6691 150 45 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6689 AflIII A'CryG_T Cuts at: 1203 2823 2825 3698 5785 6762 1203 Size: 1620 2 873 2087 977 1327 Fragments arranged by size: 2087 1620 1327 977 873 2 AgeI A'CCGG_T Cuts at: 5717 5717 Size: 6886 AhdI GACnn_n'nnGTC Cuts at: 5615 5615 Size: 6886 AleI CACnn'nnGTG Cuts at: 1166 2887 1166 Size: 1721 5165 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6886 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3000 3032 3000 Size: 32 6854 AlwNI CAG_nnn'CTG Cuts at: 2267 3585 4085 4629 6201 2267 Size: 1318 500 544 1572 2952 Fragments arranged by size: 2952 1572 1318 544 500 ApaLI G'TGCA_C Cuts at: 4943 6099 4943 Size: 1156 5730 ApoI r'AATT_y Cuts at: 1493 2842 3311 4195 4421 4573 6682 1493 Size: 1349 469 884 226 152 2109 1697 Fragments arranged by size: 2109 1697 1349 884 469 226 152 AseI AT'TA_AT Cuts at: 160 1032 5440 160 Size: 872 4408 1606 Fragments arranged by size: 4408 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6886 AvaI C'yCGr_G Cuts at: 1870 1943 4157 4211 1870 Size: 73 2214 54 4545 Fragments arranged by size: 4545 2214 73 54 BamHI G'GATC_C Cuts at: 4216 4583 4216 Size: 367 6519 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3263 4207 5662 618 Size: 325 217 627 1476 944 1455 1842 Fragments arranged by size: 1842 1476 1455 944 627 325 217 BanII G_rGCy'C Cuts at: 1391 4092 4205 1391 Size: 2701 113 4072 Fragments arranged by size: 4072 2701 113 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2231 2801 4032 928 Size: 971 332 570 1231 3782 Fragments arranged by size: 3782 1231 971 570 332 BbvCI CC'TCA_GC Cuts at: 3918 4169 3918 Size: 251 6635 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5077 5111 1903 Size: 34 3140 34 3678 Fragments arranged by size: 3678 3140 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4778 5988 4778 Size: 1210 5676 BclI T'GATC_A Cuts at: 1548 3534 1548 Size: 1986 4900 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3747 5497 136 Size: 107 122 71 631 605 2075 1750 1525 Fragments arranged by size: 2075 1750 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3856 6881 3856 Size: 3025 3861 BlpI GC'TnA_GC Cuts at: 4086 4303 4086 Size: 217 6669 Bme1580I G_kGCm'C Cuts at: 1790 2246 2978 4947 6103 1790 Size: 456 732 1969 1156 2573 Fragments arranged by size: 2573 1969 1156 732 456 BmgBI CAC'GTC Cuts at: 1318 3701 1318 Size: 2383 4503 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4617 5575 442 Size: 416 53 83 399 24 3200 958 1753 Fragments arranged by size: 3200 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6886 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2238 3147 5546 1239 Size: 999 909 2399 2579 Fragments arranged by size: 2579 2399 999 909 Bpu10I CC'TnA_GC Cuts at: 3906 3918 4169 3906 Size: 12 251 6623 Fragments arranged by size: 6623 251 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5011 5876 6174 5011 Size: 865 298 5723 Fragments arranged by size: 5723 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2872 4140 5549 882 Size: 617 1373 1268 1409 2219 Fragments arranged by size: 2219 1409 1373 1268 617 BsaAI yAC'GTr Cuts at: 493 1204 2826 4677 493 Size: 711 1622 1851 2702 Fragments arranged by size: 2702 1851 1622 711 BsaBI GATnn'nnATC Cuts at: 6880 6880 Size: 6886 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2086 4693 5075 5710 Size: 53 83 186 936 553 2607 382 635 Cuts at: 5710 275 Size: 1451 Fragments arranged by size: 2607 1451 936 635 553 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5319 5717 5991 6138 6549 5319 Size: 398 274 147 411 5656 Fragments arranged by size: 5656 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2236 2266 3000 3030 1178 Size: 30 165 30 833 30 734 30 5034 Fragments arranged by size: 5034 833 734 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 2666 3783 3893 3896 4124 1186 Size: 865 615 1117 110 3 228 3948 Fragments arranged by size: 3948 1117 865 615 228 110 3 BseYI C'CCAG_C Cuts at: 3931 4116 6089 6585 3931 Size: 185 1973 496 4232 Fragments arranged by size: 4232 1973 496 185 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3166 3175 1163 Size: 573 1430 9 4874 Fragments arranged by size: 4874 1430 573 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4336 5097 6125 1540 Size: 275 186 2335 761 1028 2301 Fragments arranged by size: 2335 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4947 5032 6103 4947 Size: 85 1071 5730 Fragments arranged by size: 5730 1071 85 BsmI GAATG_Cn' Cuts at: 2909 3382 4397 4490 2909 Size: 473 1015 93 5305 Fragments arranged by size: 5305 1015 473 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2077 2077 Size: 6886 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2246 2978 4092 4205 4947 5032 6103 Size: 399 456 732 1114 113 742 85 1071 Cuts at: 6103 1391 Size: 2174 Fragments arranged by size: 2174 1114 1071 742 732 456 399 113 85 BspEI T'CCGG_A Cuts at: 6549 6549 Size: 6886 BspHI T'CATG_A Cuts at: 3433 4775 6505 3433 Size: 1342 1730 3814 Fragments arranged by size: 3814 1730 1342 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4241 844 Size: 3397 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4773 1394 Size: 429 2950 3507 Fragments arranged by size: 3507 2950 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5381 5555 65 Size: 1183 4133 174 1396 Fragments arranged by size: 4133 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5530 5717 1700 Size: 110 3720 187 2869 Fragments arranged by size: 3720 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2176 96 Size: 2080 4806 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6886 BssSI C'ACGA_G Cuts at: 3044 4946 5958 3044 Size: 1902 1012 3972 Fragments arranged by size: 3972 1902 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2267 3153 3408 3585 3722 794 Size: 1473 886 255 177 137 3958 Fragments arranged by size: 3958 1473 886 255 177 137 BstBI TT'CG_AA Cuts at: 5690 5690 Size: 6886 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6886 BstXI CCAn_nnnn'nTGG Cuts at: 2141 2900 2141 Size: 759 6127 BstYI r'GATC_y Cuts at: 1972 2566 3856 4216 4583 4968 4985 6437 6523 Size: 594 1290 360 367 385 17 1452 86 Cuts at: 6523 6535 6881 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1290 594 385 367 360 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2930 2930 Size: 6886 BtgI C'CryG_G Cuts at: 513 1065 2004 2831 3711 513 Size: 552 939 827 880 3688 Fragments arranged by size: 3688 939 880 827 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6147 Fragments arranged by size: 6147 683 56 BtsI GCAGTG_nn' Cuts at: 2179 3583 4402 5196 5216 2179 Size: 1404 819 794 20 3849 Fragments arranged by size: 3849 1404 819 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6886 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6851 DraI TTT'AAA Cuts at: 4190 4190 Size: 6886 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5893 809 Size: 507 4577 1802 Fragments arranged by size: 4577 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3306 4333 5222 Size: 54 1425 50 275 18 1476 1027 889 Cuts at: 5222 6851 8 Size: 1629 43 Fragments arranged by size: 1629 1476 1425 1027 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4333 1537 Size: 275 2521 4090 Fragments arranged by size: 4090 2521 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3776 4101 4816 1353 Size: 2423 325 715 3423 Fragments arranged by size: 3423 2423 715 325 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3590 5473 5847 5993 387 Size: 1064 138 2001 1883 374 146 1280 Fragments arranged by size: 2001 1883 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2201 2238 2250 2327 2376 2390 2508 Size: 393 569 37 12 77 49 14 118 Cuts at: 2508 3147 4949 5546 6333 6601 1239 Size: 639 1802 597 787 268 1524 Fragments arranged by size: 1802 1524 787 639 597 569 393 268 118 77 49 37 14 12 EcoO109I rG'GnC_Cy Cuts at: 2440 2440 Size: 6886 EcoRI G'AATT_C Cuts at: 4195 4573 4195 Size: 378 6508 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2459 2567 2459 Size: 108 6778 FspI TGC'GCA Cuts at: 5392 5392 Size: 6886 HaeII r_GCGC'y Cuts at: 1678 3442 3487 3637 6033 1678 Size: 1764 45 150 2396 2531 Fragments arranged by size: 2531 2396 1764 150 45 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5527 5559 5601 5633 1373 Size: 32 135 32 3955 32 42 32 2626 Fragments arranged by size: 3955 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2167 4230 4476 669 Size: 988 281 229 2063 246 3079 Fragments arranged by size: 3079 2063 988 281 246 229 HindIII A'AGCT_T Cuts at: 748 748 Size: 6886 HpaI GTT'AAC Cuts at: 4476 4476 Size: 6886 KpnI G_GTAC'C Cuts at: 3267 4211 3267 Size: 944 5942 MfeI C'AATT_G Cuts at: 4044 4485 4044 Size: 441 6445 MluI A'CGCG_T Cuts at: 6762 6762 Size: 6886 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3683 6000 6184 1305 Size: 2378 2317 184 2007 Fragments arranged by size: 2378 2317 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3308 10 Size: 54 1425 343 1476 3588 Fragments arranged by size: 3588 1476 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2803 2871 2887 2898 3058 3272 3474 Size: 648 1637 68 16 11 160 214 202 Cuts at: 3474 3536 4844 5203 5362 518 Size: 62 1308 359 159 2042 Fragments arranged by size: 2042 1637 1308 648 359 214 202 160 159 68 62 16 11 MspA1I CmG'CkG Cuts at: 1606 2200 4124 4302 4979 6127 6372 1606 Size: 594 1924 178 677 1148 245 2120 Fragments arranged by size: 2120 1924 1148 677 594 245 178 NcoI C'CATG_G Cuts at: 513 1065 2004 3711 513 Size: 552 939 1707 3688 Fragments arranged by size: 3688 1707 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6886 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6886 NotI GC'GGCC_GC Cuts at: 4333 4333 Size: 6886 NspI r_CATG'y Cuts at: 1132 1229 3625 3725 4244 5789 1132 Size: 97 2396 100 519 1545 2229 Fragments arranged by size: 2396 2229 1545 519 100 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6886 PciI A'CATG_T Cuts at: 5785 5785 Size: 6886 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6886 PfoI T'CCnGG_A Cuts at: 2157 2157 Size: 6886 PmeI GTTT'AAAC Cuts at: 4190 4190 Size: 6886 PmlI CAC'GTG Cuts at: 2826 2826 Size: 6886 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3000 3032 4955 4987 6468 6500 3000 Size: 32 1923 32 1481 32 3386 Fragments arranged by size: 3386 1923 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6886 PsiI TTA'TAA Cuts at: 2291 4456 2291 Size: 2165 4721 PstI C_TGCA'G Cuts at: 830 3581 4238 830 Size: 2751 657 3478 Fragments arranged by size: 3478 2751 657 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6886 PvuII CAG'CTG Cuts at: 2200 4124 2200 Size: 1924 4962 SalI G'TCGA_C Cuts at: 1936 4228 1936 Size: 2292 4594 SbfI CC_TGCA'GG Cuts at: 4238 4238 Size: 6886 ScaI AGT'ACT Cuts at: 5134 6846 5134 Size: 1712 5174 SfcI C'TryA_G Cuts at: 826 1045 3577 4234 5369 6050 6241 826 Size: 219 2532 657 1135 681 191 1471 Fragments arranged by size: 2532 1471 1135 681 657 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6886 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6886 SmaI CCC'GGG Cuts at: 1872 4213 1872 Size: 2341 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4990 5891 6153 820 Size: 197 926 3047 901 262 1553 Fragments arranged by size: 3047 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4677 493 Size: 4184 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6886 SphI G_CATG'C Cuts at: 1132 4244 1132 Size: 3112 3774 SspI AAT'ATT Cuts at: 5 52 4810 5 Size: 47 4758 2081 Fragments arranged by size: 4758 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6886 StyI C'CwwG_G Cuts at: 513 1065 2004 3711 4325 513 Size: 552 939 1707 614 3074 Fragments arranged by size: 3074 1707 939 614 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3056 3350 3566 4913 4930 5083 5268 Size: 1255 1476 294 216 1347 17 153 185 Cuts at: 5268 5713 325 Size: 445 1498 Fragments arranged by size: 1498 1476 1347 1255 445 294 216 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2176 5132 6844 Size: 274 80 33 51 1443 199 2956 1712 Cuts at: 6844 96 Size: 138 Fragments arranged by size: 2956 1712 1443 274 199 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2236 2268 6846 6878 2236 Size: 32 4578 32 2244 Fragments arranged by size: 4578 2244 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3335 1316 Size: 2019 4867 XbaI T'CTAG_A Cuts at: 4222 4222 Size: 6886 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2524 3010 2524 Size: 486 6400 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6886 XmaI C'CCGG_G Cuts at: 1870 4211 1870 Size: 2341 4545 XmnI GAAnn'nnTTC Cuts at: 2403 2493 2798 3015 3053 3205 5015 2403 Size: 90 305 217 38 152 1810 4274 Fragments arranged by size: 4274 1810 305 217 152 90 38 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4694 276 Size: 53 83 186 936 3160 2468 Fragments arranged by size: 3160 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI