Restriction map for
pFN21AB5285
(Linear) (Six-base) MAPSORT of: KIBB5285.seq Check: 5000 from: 1 to: 2181
KIBB5285.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AccI GT'mk_AC
Cuts at: 0 674 1512 2181
Size: 674 838 669
Fragments arranged by size:
838 674 669
Acc65I G'GTAC_C
Cuts at: 0 1258 2181
Size: 1258 923
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 245 322 371 385 503 2181
Size: 196 49 77 49 14 118 1678
Fragments arranged by size:
1678 196 118 77 49 49 14
AfeI AGC'GCT
Cuts at: 0 1435 1480 1630 2181
Size: 1435 45 150 551
Fragments arranged by size:
1435 551 150 45
AflIII A'CryG_T
Cuts at: 0 818 820 1693 2181
Size: 818 2 873 488
Fragments arranged by size:
873 818 488 2
AleI CACnn'nnGTG
Cuts at: 0 882 2181
Size: 882 1299
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 995 1027 2181
Size: 995 32 1154
Fragments arranged by size:
1154 995 32
AlwNI CAG_nnn'CTG
Cuts at: 0 262 1580 2080 2181
Size: 262 1318 500 101
Fragments arranged by size:
1318 500 262 101
ApoI r'AATT_y
Cuts at: 0 837 1306 2181
Size: 837 469 875
Fragments arranged by size:
875 837 469
AvaI C'yCGr_G
Cuts at: 0 2152 2181
Size: 2152 29
BanI G'GyrC_C
Cuts at: 0 1258 2181
Size: 1258 923
BanII G_rGCy'C
Cuts at: 0 2087 2181
Size: 2087 94
BbsI GAAGACnn'nnnn_
Cuts at: 0 226 796 2027 2181
Size: 226 570 1231 154
Fragments arranged by size:
1231 570 226 154
BbvCI CC'TCA_GC
Cuts at: 0 1913 2164 2181
Size: 1913 251 17
Fragments arranged by size:
1913 251 17
BclI T'GATC_A
Cuts at: 0 1529 2181
Size: 1529 652
BglI GCCn_nnn'nGGC
Cuts at: 0 1742 2181
Size: 1742 439
BglII A'GATC_T
Cuts at: 0 1851 2181
Size: 1851 330
BlpI GC'TnA_GC
Cuts at: 0 2081 2181
Size: 2081 100
Bme1580I G_kGCm'C
Cuts at: 0 241 973 2181
Size: 241 732 1208
Fragments arranged by size:
1208 732 241
BmgBI CAC'GTC
Cuts at: 0 1696 2181
Size: 1696 485
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 233 1142 2181
Size: 233 909 1039
Fragments arranged by size:
1039 909 233
Bpu10I CC'TnA_GC
Cuts at: 0 1901 1913 2164 2181
Size: 1901 12 251 17
Fragments arranged by size:
1901 251 17 12
BsaI GGTCTCn'nnnn_
Cuts at: 0 867 2135 2181
Size: 867 1268 46
Fragments arranged by size:
1268 867 46
BsaAI yAC'GTr
Cuts at: 0 821 2181
Size: 821 1360
BsaHI Gr'CG_yC
Cuts at: 0 81 2181
Size: 81 2100
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 231 261 995 1025 2181
Size: 231 30 734 30 1156
Fragments arranged by size:
1156 734 231 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 661 1778 1888 1891 2119 2181
Size: 46 615 1117 110 3 228 62
Fragments arranged by size:
1117 615 228 110 62 46 3
BseYI C'CCAG_C
Cuts at: 0 1926 2111 2181
Size: 1926 185 70
Fragments arranged by size:
1926 185 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1161 1170 2181
Size: 1161 9 1011
Fragments arranged by size:
1161 1011 9
BsmI GAATG_Cn'
Cuts at: 0 904 1377 2181
Size: 904 473 804
Fragments arranged by size:
904 804 473
BsmBI CGTCTCn'nnnn_
Cuts at: 0 72 2181
Size: 72 2109
Bsp1286I G_dGCh'C
Cuts at: 0 241 973 2087 2181
Size: 241 732 1114 94
Fragments arranged by size:
1114 732 241 94
BspHI T'CATG_A
Cuts at: 0 1428 2181
Size: 1428 753
BsrGI T'GTAC_A
Cuts at: 0 171 2181
Size: 171 2010
BssSI C'ACGA_G
Cuts at: 0 1039 2181
Size: 1039 1142
BstAPI GCAn_nnn'nTGC
Cuts at: 0 262 1148 1403 1580 1717 2181
Size: 262 886 255 177 137 464
Fragments arranged by size:
886 464 262 255 177 137
BstXI CCAn_nnnn'nTGG
Cuts at: 0 136 895 2181
Size: 136 759 1286
Fragments arranged by size:
1286 759 136
BstYI r'GATC_y
Cuts at: 0 561 1851 2181
Size: 561 1290 330
Fragments arranged by size:
1290 561 330
Bsu36I CC'TnA_GG
Cuts at: 0 925 2181
Size: 925 1256
BtgI C'CryG_G
Cuts at: 0 826 1706 2181
Size: 826 880 475
Fragments arranged by size:
880 826 475
BtsI GCAGTG_nn'
Cuts at: 0 174 1578 2181
Size: 174 1404 603
Fragments arranged by size:
1404 603 174
EaeI y'GGCC_r
Cuts at: 0 1301 2181
Size: 1301 880
EarI CTCTTCn'nnn_
Cuts at: 0 1771 2096 2181
Size: 1771 325 85
Fragments arranged by size:
1771 325 85
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1585 2181
Size: 1585 596
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 233 245 322 371 385 503 1142
Size: 196 37 12 77 49 14 118 639
Cuts at: 1142 2181
Size: 1039
Fragments arranged by size:
1039 639 196 118 77 49 37 14
12
EcoO109I rG'GnC_Cy
Cuts at: 0 435 2181
Size: 435 1746
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 454 562 2181
Size: 454 108 1619
Fragments arranged by size:
1619 454 108
HaeII r_GCGC'y
Cuts at: 0 1437 1482 1632 2181
Size: 1437 45 150 549
Fragments arranged by size:
1437 549 150 45
HincII GTy'rAC
Cuts at: 0 162 2181
Size: 162 2019
KpnI G_GTAC'C
Cuts at: 0 1262 2181
Size: 1262 919
MfeI C'AATT_G
Cuts at: 0 2039 2181
Size: 2039 142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1678 2181
Size: 1678 503
MscI TGG'CCA
Cuts at: 0 1303 2181
Size: 1303 878
MslI CAynn'nnrTG
Cuts at: 0 798 866 882 893 1053 1267 1469 1531
Size: 798 68 16 11 160 214 202 62
Cuts at: 1531 2181
Size: 650
Fragments arranged by size:
798 650 214 202 160 68 62 16
11
MspA1I CmG'CkG
Cuts at: 0 195 2119 2181
Size: 195 1924 62
Fragments arranged by size:
1924 195 62
NcoI C'CATG_G
Cuts at: 0 1706 2181
Size: 1706 475
NspI r_CATG'y
Cuts at: 0 1620 1720 2181
Size: 1620 100 461
Fragments arranged by size:
1620 461 100
PfoI T'CCnGG_A
Cuts at: 0 152 2181
Size: 152 2029
PmlI CAC'GTG
Cuts at: 0 821 2181
Size: 821 1360
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 995 1027 2181
Size: 995 32 1154
Fragments arranged by size:
1154 995 32
PsiI TTA'TAA
Cuts at: 0 286 2181
Size: 286 1895
PstI C_TGCA'G
Cuts at: 0 1576 2181
Size: 1576 605
PvuII CAG'CTG
Cuts at: 0 195 2119 2181
Size: 195 1924 62
Fragments arranged by size:
1924 195 62
SfcI C'TryA_G
Cuts at: 0 1572 2181
Size: 1572 609
StyI C'CwwG_G
Cuts at: 0 1706 2181
Size: 1706 475
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1051 1345 1561 2181
Size: 1051 294 216 620
Fragments arranged by size:
1051 620 294 216
TatI w'GTAC_w
Cuts at: 0 171 2181
Size: 171 2010
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 231 263 2181
Size: 231 32 1918
Fragments arranged by size:
1918 231 32
Tth111I GACn'n_nGTC
Cuts at: 0 1330 2181
Size: 1330 851
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 519 1005 2181
Size: 519 486 1176
Fragments arranged by size:
1176 519 486
XmnI GAAnn'nnTTC
Cuts at: 0 398 488 793 1010 1048 1200 2181
Size: 398 90 305 217 38 152 981
Fragments arranged by size:
981 398 305 217 152 90 38
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AleI AloI AlwNI
ApoI AvaI BanI BanII BbsI BbvCI BclI BglI
BglII BlpI Bme1580I BmgBI BpmI Bpu10I BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsmI BsmBI Bsp1286I
BspHI BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtsI EaeI EarI EciI Eco57MI EcoO109I FalI HaeII
HincII KpnI MfeI MmeI MscI MslI MspA1I NcoI
NspI PfoI PmlI PpiI PsiI PstI PvuII SfcI
StyI TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AhdI AlfI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BcgI BciVI BfrBI BmrI BmtI BplI BpuEI BsaBI
BsaWI BsiEI BsiHKAI BsiWI BspEI BspMI BsrBI BsrDI
BsrFI BssHII BstBI BstEII BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI Hin4I HindIII HpaI KasI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PpuMI PshAI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI XbaI XhoI XmaI ZraI