Restriction map for
pFN21AB5281
(Circular) (Six-base) MAPSORT of: pFN21AB5281.seq Check: 5150 from: 1 to: 5521
pFN21AB5281 5521 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2753 2753
Size: 5521
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3331 278
Size: 53 83 186 936 1795 2468
Fragments arranged by size:
2468 1795 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2864 1937
Size: 927 4594
Acc65I G'GTAC_C
Cuts at: 2842 2842
Size: 5521
AclI AA'CG_TT
Cuts at: 1563 3648 4021 1563
Size: 2085 373 3063
Fragments arranged by size:
3063 2085 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3584 4968 5236 1632
Size: 1952 1384 268 1917
Fragments arranged by size:
1952 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5324
AflIII A'CryG_T
Cuts at: 1203 4420 5397 1203
Size: 3217 977 1327
Fragments arranged by size:
3217 1327 977
AgeI A'CCGG_T
Cuts at: 4352 4352
Size: 5521
AhdI GACnn_n'nnGTC
Cuts at: 4250 4250
Size: 5521
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5521
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2525 1184
Size: 1341 4180
AlwNI CAG_nnn'CTG
Cuts at: 2323 2368 2518 3264 4836 2323
Size: 45 150 746 1572 3008
Fragments arranged by size:
3008 1572 746 150 45
ApaI G_GGCC'C
Cuts at: 2259 2550 2259
Size: 291 5230
ApaLI G'TGCA_C
Cuts at: 3578 4734 3578
Size: 1156 4365
ApoI r'AATT_y
Cuts at: 1493 2830 3056 3208 5317 1493
Size: 1337 226 152 2109 1697
Fragments arranged by size:
2109 1697 1337 226 152
AscI GG'CGCG_CC
Cuts at: 2433 2433
Size: 5521
AseI AT'TA_AT
Cuts at: 160 1032 4075 160
Size: 872 3043 1606
Fragments arranged by size:
3043 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5521
AvaI C'yCGr_G
Cuts at: 1870 1943 2045 2087 2252 2258 2275 2846 1870
Size: 73 102 42 165 6 17 571 4545
Fragments arranged by size:
4545 571 165 102 73 42 17 6
BamHI G'GATC_C
Cuts at: 2851 3218 2851
Size: 367 5154
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2230 2540 2617 2624 2842
Size: 325 217 627 443 310 77 7 218
Cuts at: 2842 4297 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 443 325 310 218 217
77 7
BanII G_rGCy'C
Cuts at: 1391 2259 2550 2840 1391
Size: 868 291 290 4072
Fragments arranged by size:
4072 868 291 290
BbeI G_GCGC'C
Cuts at: 2234 2544 2621 2628 2234
Size: 310 77 7 5127
Fragments arranged by size:
5127 310 77 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4550
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3712 3746 1903
Size: 34 1775 34 3678
Fragments arranged by size:
3678 1775 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3413 4623 3413
Size: 1210 4311
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5521
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2068 2804 4132
Size: 107 122 71 631 605 396 736 1328
Cuts at: 4132 136
Size: 1525
Fragments arranged by size:
1525 1328 736 631 605 396 122 107
71
BglII A'GATC_T
Cuts at: 5516 5516
Size: 5521
BlpI GC'TnA_GC
Cuts at: 2938 2938
Size: 5521
Bme1580I G_kGCm'C
Cuts at: 1790 2259 2550 3582 4738 1790
Size: 469 291 1032 1156 2573
Fragments arranged by size:
2573 1156 1032 469 291
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5521
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3252 4210 442
Size: 416 53 83 399 24 1835 958 1753
Fragments arranged by size:
1835 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5521
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2229 4181 1239
Size: 990 1952 2579
Fragments arranged by size:
2579 1952 990
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3646 4511 4809 3646
Size: 865 298 4358
Fragments arranged by size:
4358 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4184 882
Size: 617 2685 2219
Fragments arranged by size:
2685 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3312 493
Size: 711 2108 2702
Fragments arranged by size:
2702 2108 711
BsaBI GATnn'nnATC
Cuts at: 5515 5515
Size: 5521
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2231 2298 2385 2541
Size: 53 83 186 936 698 67 87 156
Cuts at: 2541 2618 2625 3328 3710 4345 275
Size: 77 7 703 382 635 1451
Fragments arranged by size:
1451 936 703 698 635 382 186 156
87 83 77 67 53 7
BsaWI w'CCGG_w
Cuts at: 3954 4352 4626 4773 5184 3954
Size: 398 274 147 411 4291
Fragments arranged by size:
4291 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5296
Fragments arranged by size:
5296 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2122 2125 2128 2131 2134 2137 2146 2155
Size: 936 3 3 3 3 3 9 9
Cuts at: 2155 2167 2170 2189 2321 1186
Size: 12 3 19 132 4386
Fragments arranged by size:
4386 936 132 19 12 9 9 3
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2497 2530 4724 5220 2497
Size: 33 2194 496 2798
Fragments arranged by size:
2798 2194 496 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2725 2822 1163
Size: 573 989 97 3862
Fragments arranged by size:
3862 989 573 97
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2238 2294 2443 2971 3732 4760
Size: 275 186 237 56 149 528 761 1028
Cuts at: 4760 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 528 275 237 186 149
56
BsiHKAI G_wGCw'C
Cuts at: 3582 3667 4738 3582
Size: 85 1071 4365
Fragments arranged by size:
4365 1071 85
BsmI GAATG_Cn'
Cuts at: 3032 3125 3032
Size: 93 5428
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2259 2550 2840 3582 3667 4738 1391
Size: 399 469 291 290 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 469 399 291 290 85
BspEI T'CCGG_A
Cuts at: 5184 5184
Size: 5521
BspHI T'CATG_A
Cuts at: 3410 5140 3410
Size: 1730 3791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2753 2876 844
Size: 1909 123 3489
Fragments arranged by size:
3489 1909 123
BsrBI CCG'CTC
Cuts at: 1394 1823 2350 3408 1394
Size: 429 527 1058 3507
Fragments arranged by size:
3507 1058 527 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4016 4190 65
Size: 1183 2768 174 1396
Fragments arranged by size:
2768 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2233 4165 4352 1700
Size: 110 423 1932 187 2869
Fragments arranged by size:
2869 1932 423 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5521
BssHII G'CGCG_C
Cuts at: 1925 2027 2213 2268 2433 2526 1925
Size: 102 186 55 165 93 4920
Fragments arranged by size:
4920 186 165 102 93 55
BssSI C'ACGA_G
Cuts at: 3581 4593 3581
Size: 1012 4509
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2323 2518 794
Size: 1529 195 3797
Fragments arranged by size:
3797 1529 195
BstBI TT'CG_AA
Cuts at: 4325 4325
Size: 5521
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5521
BstYI r'GATC_y
Cuts at: 1972 2851 3218 3603 3620 5072 5158 5170 5516
Size: 879 367 385 17 1452 86 12 346
Cuts at: 5516 1972
Size: 1977
Fragments arranged by size:
1977 1452 879 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2379 513
Size: 552 1314 3655
Fragments arranged by size:
3655 1314 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4782
Fragments arranged by size:
4782 683 56
BtsI GCAGTG_nn'
Cuts at: 2422 3037 3831 3851 2422
Size: 615 794 20 4092
Fragments arranged by size:
4092 794 615 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5521
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5486
DraI TTT'AAA
Cuts at: 2825 2825
Size: 5521
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4528 809
Size: 507 3212 1802
Fragments arranged by size:
3212 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2060 2235 2291
Size: 54 1425 50 275 18 230 175 56
Cuts at: 2291 2376 2440 2571 2695 2778 2793 2968 3857
Size: 85 64 131 124 83 15 175 889
Cuts at: 3857 5486 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 275 230 175 175 131
124 85 83 64 56 54 50 43
18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2235 2291 2440 2968 1537
Size: 275 423 56 149 528 4090
Fragments arranged by size:
4090 528 423 275 149 56
EarI CTCTTCn'nnn_
Cuts at: 1353 3451 1353
Size: 2098 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4108 4482 4628 387
Size: 1064 138 2519 374 146 1280
Fragments arranged by size:
2519 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2229 3584 4181 4968 5236 1239
Size: 393 597 1355 597 787 268 1524
Fragments arranged by size:
1524 1355 787 597 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2204 2393 2204
Size: 189 5332
EcoO109I rG'GnC_Cy
Cuts at: 2091 2249 2365 2633 2091
Size: 158 116 268 4979
Fragments arranged by size:
4979 268 158 116
EcoRI G'AATT_C
Cuts at: 2830 3208 2830
Size: 378 5143
FspI TGC'GCA
Cuts at: 4027 4027
Size: 5521
HaeII r_GCGC'y
Cuts at: 1678 2234 2289 2544 2621 2628 2678 4668 1678
Size: 556 55 255 77 7 50 1990 2531
Fragments arranged by size:
2531 1990 556 255 77 55 50 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4162 4194 4236 4268 1373
Size: 32 135 32 2590 32 42 32 2626
Fragments arranged by size:
2626 2590 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2865 3111 669
Size: 988 281 927 246 3079
Fragments arranged by size:
3079 988 927 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5521
HpaI GTT'AAC
Cuts at: 3111 3111
Size: 5521
KasI G'GCGC_C
Cuts at: 2230 2540 2617 2624 2230
Size: 310 77 7 5127
Fragments arranged by size:
5127 310 77 7
KpnI G_GTAC'C
Cuts at: 2846 2846
Size: 5521
MfeI C'AATT_G
Cuts at: 3120 3120
Size: 5521
MluI A'CGCG_T
Cuts at: 5397 5397
Size: 5521
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4635 4819 1305
Size: 3330 184 2007
Fragments arranged by size:
3330 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 10
Size: 54 1425 343 546 3153
Fragments arranged by size:
3153 1425 546 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3479 3838 3997 518
Size: 648 2313 359 159 2042
Fragments arranged by size:
2313 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2068 2323 2417 2937 3614 4762 5007 1606
Size: 462 255 94 520 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 520 462 255 245 94
NaeI GCC'GGC
Cuts at: 2235 2235
Size: 5521
NarI GG'CG_CC
Cuts at: 2231 2541 2618 2625 2231
Size: 310 77 7 5127
Fragments arranged by size:
5127 310 77 7
NcoI C'CATG_G
Cuts at: 513 1065 2379 513
Size: 552 1314 3655
Fragments arranged by size:
3655 1314 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5521
NgoMIV G'CCGG_C
Cuts at: 2233 2233
Size: 5521
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5521
NotI GC'GGCC_GC
Cuts at: 2968 2968
Size: 5521
NspI r_CATG'y
Cuts at: 1132 1229 2362 2879 4424 1132
Size: 97 1133 517 1545 2229
Fragments arranged by size:
2229 1545 1133 517 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5521
PciI A'CATG_T
Cuts at: 4420 4420
Size: 5521
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5521
PmeI GTTT'AAAC
Cuts at: 2825 2825
Size: 5521
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3590 3622 5103 5135 3590
Size: 32 1481 32 3976
Fragments arranged by size:
3976 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2365 2633 2365
Size: 268 5253
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5521
PsiI TTA'TAA
Cuts at: 3091 3091
Size: 5521
PspOMI G'GGCC_C
Cuts at: 2255 2546 2255
Size: 291 5230
PstI C_TGCA'G
Cuts at: 830 2514 2873 830
Size: 1684 359 3478
Fragments arranged by size:
3478 1684 359
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5521
PvuII CAG'CTG
Cuts at: 2323 2323
Size: 5521
SalI G'TCGA_C
Cuts at: 1936 2863 1936
Size: 927 4594
SbfI CC_TGCA'GG
Cuts at: 2873 2873
Size: 5521
ScaI AGT'ACT
Cuts at: 3769 5481 3769
Size: 1712 3809
SexAI A'CCwGG_T
Cuts at: 2769 2769
Size: 5521
SfcI C'TryA_G
Cuts at: 826 1045 2116 2510 2869 4004 4685 4876 826
Size: 219 1071 394 359 1135 681 191 1471
Fragments arranged by size:
1471 1135 1071 681 394 359 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2068 2068
Size: 5521
SfoI GGC'GCC
Cuts at: 2232 2542 2619 2626 2232
Size: 310 77 7 5127
Fragments arranged by size:
5127 310 77 7
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5521
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5521
SmaI CCC'GGG
Cuts at: 1872 2047 2254 2260 2277 2848 1872
Size: 175 207 6 17 571 4545
Fragments arranged by size:
4545 571 207 175 17 6
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3625 4526 4788 820
Size: 197 926 1682 901 262 1553
Fragments arranged by size:
1682 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3312 493
Size: 2819 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5521
SphI G_CATG'C
Cuts at: 1132 2362 2879 1132
Size: 1230 517 3774
Fragments arranged by size:
3774 1230 517
SspI AAT'ATT
Cuts at: 5 52 3445 5
Size: 47 3393 2081
Fragments arranged by size:
3393 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5521
StyI C'CwwG_G
Cuts at: 513 1065 2205 2301 2379 2817 2960 513
Size: 552 1140 96 78 438 143 3074
Fragments arranged by size:
3074 1140 552 438 143 96 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3548 3565 3718 3903 4348 325
Size: 1255 1968 17 153 185 445 1498
Fragments arranged by size:
1968 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 3767 5479
Size: 274 80 33 51 1443 30 1760 1712
Cuts at: 5479 96
Size: 138
Fragments arranged by size:
1760 1712 1443 274 138 80 51 33
30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5481 5513 5481
Size: 32 5489
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5521
XbaI T'CTAG_A
Cuts at: 2857 2857
Size: 5521
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2788 2788
Size: 5521
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5521
XmaI C'CCGG_G
Cuts at: 1870 2045 2252 2258 2275 2846 1870
Size: 175 207 6 17 571 4545
Fragments arranged by size:
4545 571 207 175 17 6
XmnI GAAnn'nnTTC
Cuts at: 3650 3650
Size: 5521
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3329 276
Size: 53 83 186 936 1795 2468
Fragments arranged by size:
2468 1795 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SacI SacII SanDI SapI SrfI
SwaI