Restriction map for
pFN21AB5281
(Linear) (Six-base) MAPSORT of: KIBB5281.seq Check: 8372 from: 1 to: 816
KIBB5281.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 748 816
Size: 748 68
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 520 816
Size: 520 296
AlwNI CAG_nnn'CTG
Cuts at: 0 318 363 513 816
Size: 318 45 150 303
Fragments arranged by size:
318 303 150 45
ApaI G_GGCC'C
Cuts at: 0 254 545 816
Size: 254 291 271
Fragments arranged by size:
291 271 254
AscI GG'CGCG_CC
Cuts at: 0 428 816
Size: 428 388
AvaI C'yCGr_G
Cuts at: 0 40 82 247 253 270 816
Size: 40 42 165 6 17 546
Fragments arranged by size:
546 165 42 40 17 6
BanI G'GyrC_C
Cuts at: 0 225 535 612 619 816
Size: 225 310 77 7 197
Fragments arranged by size:
310 225 197 77 7
BanII G_rGCy'C
Cuts at: 0 254 545 816
Size: 254 291 271
Fragments arranged by size:
291 271 254
BbeI G_GCGC'C
Cuts at: 0 229 539 616 623 816
Size: 229 310 77 7 193
Fragments arranged by size:
310 229 193 77 7
BglI GCCn_nnn'nGGC
Cuts at: 0 63 799 816
Size: 63 736 17
Fragments arranged by size:
736 63 17
Bme1580I G_kGCm'C
Cuts at: 0 254 545 816
Size: 254 291 271
Fragments arranged by size:
291 271 254
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 816
Size: 224 592
BsaHI Gr'CG_yC
Cuts at: 0 226 293 380 536 613 620 816
Size: 226 67 87 156 77 7 196
Fragments arranged by size:
226 196 156 87 77 67 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 117 120 123 126 129 132 141 150
Size: 117 3 3 3 3 3 9 9
Cuts at: 150 162 165 184 316 816
Size: 12 3 19 132 500
Fragments arranged by size:
500 132 117 19 12 9 9 3
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 492 525 816
Size: 492 33 291
Fragments arranged by size:
492 291 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 720 816
Size: 720 96
BsiEI CG_ry'CG
Cuts at: 0 233 289 438 816
Size: 233 56 149 378
Fragments arranged by size:
378 233 149 56
Bsp1286I G_dGCh'C
Cuts at: 0 254 545 816
Size: 254 291 271
Fragments arranged by size:
291 271 254
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 748 816
Size: 748 68
BsrBI CCG'CTC
Cuts at: 0 345 816
Size: 345 471
BsrFI r'CCGG_y
Cuts at: 0 228 816
Size: 228 588
BssHII G'CGCG_C
Cuts at: 0 22 208 263 428 521 816
Size: 22 186 55 165 93 295
Fragments arranged by size:
295 186 165 93 55 22
BstAPI GCAn_nnn'nTGC
Cuts at: 0 318 513 816
Size: 318 195 303
Fragments arranged by size:
318 303 195
BtgI C'CryG_G
Cuts at: 0 374 816
Size: 374 442
BtsI GCAGTG_nn'
Cuts at: 0 417 816
Size: 417 399
EaeI y'GGCC_r
Cuts at: 0 55 230 286 371 435 566 690 773
Size: 55 175 56 85 64 131 124 83
Cuts at: 773 788 816
Size: 15 28
Fragments arranged by size:
175 131 124 85 83 64 56 55
28 15
EagI C'GGCC_G
Cuts at: 0 230 286 435 816
Size: 230 56 149 381
Fragments arranged by size:
381 230 149 56
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 816
Size: 224 592
EcoNI CCTnn'n_nnAGG
Cuts at: 0 199 388 816
Size: 199 189 428
Fragments arranged by size:
428 199 189
EcoO109I rG'GnC_Cy
Cuts at: 0 86 244 360 628 816
Size: 86 158 116 268 188
Fragments arranged by size:
268 188 158 116 86
HaeII r_GCGC'y
Cuts at: 0 229 284 539 616 623 673 816
Size: 229 55 255 77 7 50 143
Fragments arranged by size:
255 229 143 77 55 50 7
KasI G'GCGC_C
Cuts at: 0 225 535 612 619 816
Size: 225 310 77 7 197
Fragments arranged by size:
310 225 197 77 7
MscI TGG'CCA
Cuts at: 0 373 816
Size: 373 443
MspA1I CmG'CkG
Cuts at: 0 63 318 412 816
Size: 63 255 94 404
Fragments arranged by size:
404 255 94 63
NaeI GCC'GGC
Cuts at: 0 230 816
Size: 230 586
NarI GG'CG_CC
Cuts at: 0 226 536 613 620 816
Size: 226 310 77 7 196
Fragments arranged by size:
310 226 196 77 7
NcoI C'CATG_G
Cuts at: 0 374 816
Size: 374 442
NgoMIV G'CCGG_C
Cuts at: 0 228 816
Size: 228 588
NspI r_CATG'y
Cuts at: 0 357 816
Size: 357 459
PpuMI rG'GwC_Cy
Cuts at: 0 360 628 816
Size: 360 268 188
Fragments arranged by size:
360 268 188
PspOMI G'GGCC_C
Cuts at: 0 250 541 816
Size: 250 291 275
Fragments arranged by size:
291 275 250
PstI C_TGCA'G
Cuts at: 0 509 816
Size: 509 307
PvuII CAG'CTG
Cuts at: 0 318 816
Size: 318 498
SexAI A'CCwGG_T
Cuts at: 0 764 816
Size: 764 52
SfcI C'TryA_G
Cuts at: 0 111 505 816
Size: 111 394 311
Fragments arranged by size:
394 311 111
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 63 816
Size: 63 753
SfoI GGC'GCC
Cuts at: 0 227 537 614 621 816
Size: 227 310 77 7 195
Fragments arranged by size:
310 227 195 77 7
SmaI CCC'GGG
Cuts at: 0 42 249 255 272 816
Size: 42 207 6 17 544
Fragments arranged by size:
544 207 42 17 6
SphI G_CATG'C
Cuts at: 0 357 816
Size: 357 459
StyI C'CwwG_G
Cuts at: 0 200 296 374 816
Size: 200 96 78 442
Fragments arranged by size:
442 200 96 78
TatI w'GTAC_w
Cuts at: 0 2 816
Size: 2 814
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 783 816
Size: 783 33
XmaI C'CCGG_G
Cuts at: 0 40 247 253 270 816
Size: 40 207 6 17 546
Fragments arranged by size:
546 207 40 17 6
Enzymes that do cut:
AarI AlfI AlwNI ApaI AscI AvaI BanI BanII
BbeI BglI Bme1580I BpmI BsaHI BseRI BseYI BsgI
BsiEI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BtgI
BtsI EaeI EagI Eco57MI EcoNI EcoO109I HaeII KasI
MscI MspA1I NaeI NarI NcoI NgoMIV NspI PpuMI
PspOMI PstI PvuII SexAI SfcI SfiI SfoI SmaI
SphI StyI TatI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaLI ApoI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EarI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MslI NdeI NheI NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SgfI
SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI
TaqII TstI Tth111I XbaI XhoI XmnI ZraI