Restriction map for
pFN21AB5144
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BanI
BbeI BbrPI BbvCI BcnI BfrI
BglI BglII BlnI BsaAI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsoBI Bsp1407I BspDI
BspEI BspMI BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Cac8I CfoI
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV FseI FspI
HaeII HhaI Hin1I HinP1I HincII
HindII HpaI KasI KpnI KspI
MluI MroI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AflIII 851 ACATGT A^C[GA][CT]GT
AocI 926 CCTTAGG CC^T[ACGT]AGG
BalI 126 TGGCCA TGG^CCA
BamHI 2098 GGATCC G^GATCC
BanII 883 GAGCCC G[GA]GC[CT]^C
BcgI 2045 CGAGCGAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2112 GGATAC GTATCC(6/5)
BclI 728 TGATCA T^GATCA
BlpI 1319 GCTTAGC GC^T[ACGT]AGC
BmyI 883 GAGCCC G[AGT]GC[ACT]^C
Bpu1102I 1319 GCTTAGC GC^T[ACGT]AGC
BsaBI 681 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1730 CTCCTC GAGGAG(10/8)
BsmBI 1117 GAGACG CGTCTC(1/5)
Bsp1286I 883 GAGCCC G[AGT]GC[ACT]^C
BspHI 1124 TCATGA T^CATGA
BspLU11I 851 ACATGT A^CATGT
BsrFI 1315 ACCGGC [GA]^CCGG[CT]
BstAPI 522 GCAACCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 210 CATCC GGATG(2/0)
Bsu36I 926 CCTTAGG CC^T[ACGT]AGG
CelII 1319 GCTTAGC GC^T[ACGT]AGC
Cfr10I 1315 ACCGGC [GA]^CCGG[CT]
DraI 822 TTTAAA TTT^AAA
DraII 1982 GGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 126 TGGCCA [CT]^GGCC[GA]
Eco81I 926 CCTTAGG CC^T[ACGT]AGG
EcoO109I 1982 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 371 GAATTC G^AATTC
EcoT22I 1098 ATGCAT ATGCA^T
FauI 422 GCGGG CCCGC(4/6)
FbaI 728 TGATCA T^GATCA
FokI 210 CATCC GGATG(9/13)
HapII 1316 CCGG C^CGG
HgaI 82 GACGC GACGC(5/10)
HindIII 865 AAGCTT A^AGCTT
HpaII 1316 CCGG C^CGG
HphI 552 GGTGA GGTGA(8/7)
MaeII 476 ACGT A^CGT
MamI 681 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 833 CAATTG C^AATTG
MluNI 126 TGGCCA TGG^CCA
MscI 126 TGGCCA TGG^CCA
MspA1I 86 CAGCTG C[AC]G^C[GT]G
MspI 1316 CCGG C^CGG
MunI 833 CAATTG C^AATTG
NdeI 782 CATATG CA^TATG
NsiI 1098 ATGCAT ATGCA^T
NspI 851 ACATGT [GA]CATG^[CT]
PleI 52 GAGTC GAGTC(4/5)
PpuMI 1982 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 1196 CTGCAG CTGCA^G
PvuII 86 CAGCTG CAG^CTG
RcaI 1124 TCATGA T^CATGA
SmlI 911 CTCAAG C^T[CT][GA]AG
StuI 1410 AGGCCT AGG^CCT
TfiI 2179 GAATC G^A[AT]TC
XcmI 686 CCATCTGCTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 287 GCGG CCGC(-3/-1)
422 GCGG
AlwNI 1895 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
2177 CAGAATCTG
Asp700I 1246 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1825 GAAGAATTTC
AvaII 1313 GGACC G^G[AT]CC
1983 GGTCC
BfaI 1408 CTAG C^TAG
1760 CTAG
BsrDI 951 CATTGC GCAATG(2/0)
1748 CATTGC
BstYI 160 GGATCT [GA]^GATC[CT]
2098 GGATCC
HinfI 52 GAGTC G^A[ACGT]TC
2179 GAATC
MaeI 1408 CTAG C^TAG
1760 CTAG
MflI 160 GGATCT [GA]^GATC[CT]
2098 GGATCC
MslI 263 CATACCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
991 CACTTTGATG
PflMI 887 CCAAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2062 CCAAATTTTGG
SapI 1288 GAAGAGC GCTCTTC(1/4)
1258 GCTCTTC
TaqI 170 TCGA T^CGA
2085 TCGA
Tsp45I 1447 GTCAC ^GT[GC]AC
1963 GTGAC
TthHB8I 170 TCGA T^CGA
2085 TCGA
Van91I 887 CCAAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2062 CCAAATTTTGG
XhoII 160 GGATCT [GA]^GATC[CT]
2098 GGATCC
XmnI 1246 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1825 GAAGAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbsI BbvI BpmI BpuAI BsaJI
BsiYI BslI BsmAI BsmFI BsmI
BsrI BssKI BstNI Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
HaeIII ItaI Ksp632I MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
StyI Tru9I TseI Tsp509I TspRI