(Circular) (Six-base) MAPSORT of: pFN21AB5144.seq Check: 3144 from: 1 to: 6895 pFN21AB5144 6895 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4705 278 Size: 53 83 186 936 3169 2468 Fragments arranged by size: 3169 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4238 1937 Size: 2301 4594 Acc65I G'GTAC_C Cuts at: 4216 4216 Size: 6895 AclI AA'CG_TT Cuts at: 1563 5022 5395 1563 Size: 3459 373 3063 Fragments arranged by size: 3459 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2190 3849 4958 6342 6610 1632 Size: 558 1659 1109 1384 268 1917 Fragments arranged by size: 1917 1659 1384 1109 558 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6698 AflIII A'CryG_T Cuts at: 1203 2856 5794 6771 1203 Size: 1653 2938 977 1327 Fragments arranged by size: 2938 1653 1327 977 AgeI A'CCGG_T Cuts at: 5726 5726 Size: 6895 AhdI GACnn_n'nnGTC Cuts at: 5624 5624 Size: 6895 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6895 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6895 AlwNI CAG_nnn'CTG Cuts at: 3905 4187 4638 6210 3905 Size: 282 451 1572 4590 Fragments arranged by size: 4590 1572 451 282 ApaLI G'TGCA_C Cuts at: 4952 6108 4952 Size: 1156 5739 ApoI r'AATT_y Cuts at: 1493 2376 3115 3134 3489 3833 4069 4204 4430 Size: 883 739 19 355 344 236 135 226 Cuts at: 4430 4582 6691 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 883 739 355 344 236 226 152 135 19 AseI AT'TA_AT Cuts at: 160 1032 5449 160 Size: 872 4417 1606 Fragments arranged by size: 4417 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6895 AvaI C'yCGr_G Cuts at: 1870 1943 4220 1870 Size: 73 2277 4545 Fragments arranged by size: 4545 2277 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2139 2172 2139 Size: 33 6862 BamHI G'GATC_C Cuts at: 4103 4225 4592 4103 Size: 122 367 6406 Fragments arranged by size: 6406 367 122 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4216 5671 618 Size: 325 217 627 2429 1455 1842 Fragments arranged by size: 2429 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2892 4214 1391 Size: 1501 1322 4072 Fragments arranged by size: 4072 1501 1322 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2091 2742 3503 3675 928 Size: 971 192 651 761 172 4148 Fragments arranged by size: 4148 971 761 651 192 172 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4039 4073 5086 5120 1903 Size: 34 50 34 2018 34 1013 34 3678 Fragments arranged by size: 3678 2018 1013 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4111 4787 5997 4111 Size: 676 1210 5009 Fragments arranged by size: 5009 1210 676 BclI T'GATC_A Cuts at: 1548 2733 1548 Size: 1185 5710 BfrBI ATG'CAT Cuts at: 3105 3105 Size: 6895 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5506 136 Size: 107 122 71 631 605 3834 1525 Fragments arranged by size: 3834 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6890 6890 Size: 6895 BlpI GC'TnA_GC Cuts at: 3325 4312 3325 Size: 987 5908 Bme1580I G_kGCm'C Cuts at: 1790 4956 6112 1790 Size: 3166 1156 2573 Fragments arranged by size: 3166 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6895 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2432 3893 4626 Size: 416 53 83 399 24 1015 1461 733 Cuts at: 4626 5584 442 Size: 958 1753 Fragments arranged by size: 1753 1461 1015 958 733 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6895 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2515 2906 2920 5555 1239 Size: 1276 391 14 2635 2579 Fragments arranged by size: 2635 2579 1276 391 14 Bpu10I CC'TnA_GC Cuts at: 2868 2868 Size: 6895 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2901 5020 5885 6183 2901 Size: 2119 865 298 3613 Fragments arranged by size: 3613 2119 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5558 882 Size: 617 4059 2219 Fragments arranged by size: 4059 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4686 493 Size: 711 3482 2702 Fragments arranged by size: 3482 2702 711 BsaBI GATnn'nnATC Cuts at: 2690 6889 2690 Size: 4199 2696 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4702 5084 5719 275 Size: 53 83 186 936 3169 382 635 1451 Fragments arranged by size: 3169 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5328 5726 6000 6147 6558 5328 Size: 398 274 147 411 5665 Fragments arranged by size: 5665 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6670 Fragments arranged by size: 6670 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3726 1186 Size: 2540 4355 BseYI C'CCAG_C Cuts at: 3685 3698 6098 6594 3685 Size: 13 2400 496 3986 Fragments arranged by size: 3986 2400 496 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6322 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4345 5106 6134 1540 Size: 275 186 2344 761 1028 2301 Fragments arranged by size: 2344 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4956 5041 6112 4956 Size: 85 1071 5739 Fragments arranged by size: 5739 1071 85 BsmI GAATG_Cn' Cuts at: 2742 2819 2847 4406 4499 2742 Size: 77 28 1559 93 5138 Fragments arranged by size: 5138 1559 93 77 28 BsmBI CGTCTCn'nnnn_ Cuts at: 3116 3116 Size: 6895 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2892 4214 4956 5041 6112 1391 Size: 399 1102 1322 742 85 1071 2174 Fragments arranged by size: 2174 1322 1102 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6558 6558 Size: 6895 BspHI T'CATG_A Cuts at: 3129 4784 6514 3129 Size: 1655 1730 3510 Fragments arranged by size: 3510 1730 1655 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4250 844 Size: 3406 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4782 1394 Size: 429 2959 3507 Fragments arranged by size: 3507 2959 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2955 3752 5390 5564 65 Size: 1183 1707 797 1638 174 1396 Fragments arranged by size: 1707 1638 1396 1183 797 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3320 5539 5726 1700 Size: 110 1510 2219 187 2869 Fragments arranged by size: 2869 2219 1510 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6895 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6895 BssSI C'ACGA_G Cuts at: 4955 5967 4955 Size: 1012 5883 BstAPI GCAn_nnn'nTGC Cuts at: 794 2533 794 Size: 1739 5156 BstBI TT'CG_AA Cuts at: 5699 5699 Size: 6895 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6895 BstYI r'GATC_y Cuts at: 1972 2165 4103 4225 4592 4977 4994 6446 6532 Size: 193 1938 122 367 385 17 1452 86 Cuts at: 6532 6544 6890 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1938 1452 385 367 346 193 122 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2932 2932 Size: 6895 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6343 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3365 4032 487 Size: 683 56 2139 667 3350 Fragments arranged by size: 3350 2139 683 667 56 BtsI GCAGTG_nn' Cuts at: 3593 4411 5205 5225 3593 Size: 818 794 20 5263 Fragments arranged by size: 5263 818 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6895 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3036 3071 529 Size: 35 2472 35 4353 Fragments arranged by size: 4353 2472 35 35 DraI TTT'AAA Cuts at: 2829 4199 2829 Size: 1370 5525 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5902 809 Size: 507 4586 1802 Fragments arranged by size: 4586 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2131 4342 5231 Size: 54 1425 50 275 18 301 2211 889 Cuts at: 5231 6860 8 Size: 1629 43 Fragments arranged by size: 2211 1629 1425 889 301 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4342 1537 Size: 275 2530 4090 Fragments arranged by size: 4090 2530 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2133 3246 3270 3288 4825 1353 Size: 780 1113 24 18 1537 3423 Fragments arranged by size: 3423 1537 1113 780 24 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5482 5856 6002 387 Size: 1064 138 3893 374 146 1280 Fragments arranged by size: 3893 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2190 2515 2906 2920 3849 4958 5555 Size: 393 558 325 391 14 929 1109 597 Cuts at: 5555 6342 6610 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1109 929 787 597 558 393 391 325 268 14 EcoO109I rG'GnC_Cy Cuts at: 3988 3988 Size: 6895 EcoRI G'AATT_C Cuts at: 2376 4204 4582 2376 Size: 1828 378 4689 Fragments arranged by size: 4689 1828 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2090 2208 3509 2090 Size: 118 1301 5476 Fragments arranged by size: 5476 1301 118 FspI TGC'GCA Cuts at: 5401 5401 Size: 6895 HaeII r_GCGC'y Cuts at: 1678 6042 1678 Size: 4364 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2157 2189 3809 3841 3972 Size: 32 135 32 585 32 1620 32 131 Cuts at: 3972 4004 4046 4048 4078 4080 5536 5568 5610 Size: 32 42 2 30 2 1456 32 42 Cuts at: 5610 5642 1373 Size: 32 2626 Fragments arranged by size: 2626 1620 1456 585 135 131 42 42 32 32 32 32 32 32 32 30 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 4239 4485 669 Size: 988 281 2301 246 3079 Fragments arranged by size: 3079 2301 988 281 246 HindIII A'AGCT_T Cuts at: 748 2870 748 Size: 2122 4773 HpaI GTT'AAC Cuts at: 4485 4485 Size: 6895 KpnI G_GTAC'C Cuts at: 4220 4220 Size: 6895 MfeI C'AATT_G Cuts at: 2838 4494 2838 Size: 1656 5239 MluI A'CGCG_T Cuts at: 6771 6771 Size: 6895 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2209 3872 6009 6193 1305 Size: 904 1663 2137 184 2007 Fragments arranged by size: 2137 2007 1663 904 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2133 10 Size: 54 1425 343 301 4772 Fragments arranged by size: 4772 1425 343 301 54 MslI CAynn'nnrTG Cuts at: 518 1166 2272 3000 4853 5212 5371 518 Size: 648 1106 728 1853 359 159 2042 Fragments arranged by size: 2042 1853 1106 728 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2093 4311 4988 6136 6381 1606 Size: 487 2218 677 1148 245 2120 Fragments arranged by size: 2218 2120 1148 677 487 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6343 NdeI CA'TA_TG Cuts at: 387 2788 387 Size: 2401 4494 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6895 NotI GC'GGCC_GC Cuts at: 4342 4342 Size: 6895 NsiI A_TGCA'T Cuts at: 3107 3107 Size: 6895 NspI r_CATG'y Cuts at: 1132 1229 2860 4253 5798 1132 Size: 97 1631 1393 1545 2229 Fragments arranged by size: 2229 1631 1545 1393 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6895 PciI A'CATG_T Cuts at: 2856 5794 2856 Size: 2938 3957 PflMI CCAn_nnn'nTGG Cuts at: 1108 2898 4073 1108 Size: 1790 1175 3930 Fragments arranged by size: 3930 1790 1175 PmeI GTTT'AAAC Cuts at: 4199 4199 Size: 6895 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4964 4996 6477 6509 4964 Size: 32 1481 32 5350 Fragments arranged by size: 5350 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3988 3988 Size: 6895 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6895 PsiI TTA'TAA Cuts at: 4465 4465 Size: 6895 PstI C_TGCA'G Cuts at: 830 3205 4247 830 Size: 2375 1042 3478 Fragments arranged by size: 3478 2375 1042 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6895 PvuII CAG'CTG Cuts at: 2093 2093 Size: 6895 SalI G'TCGA_C Cuts at: 1936 4237 1936 Size: 2301 4594 SapI GCTCTTCn'nnn_ Cuts at: 3270 3288 3270 Size: 18 6877 SbfI CC_TGCA'GG Cuts at: 4247 4247 Size: 6895 ScaI AGT'ACT Cuts at: 5143 6855 5143 Size: 1712 5183 SfcI C'TryA_G Cuts at: 826 1045 2238 3201 3906 4243 5378 6059 6250 Size: 219 1193 963 705 337 1135 681 191 Cuts at: 6250 826 Size: 1471 Fragments arranged by size: 1471 1193 1135 963 705 681 337 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6895 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6895 SmaI CCC'GGG Cuts at: 1872 4222 1872 Size: 2350 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2916 4999 5900 6162 820 Size: 197 926 973 2083 901 262 1553 Fragments arranged by size: 2083 1553 973 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4686 493 Size: 4193 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6895 SphI G_CATG'C Cuts at: 1132 4253 1132 Size: 3121 3774 SspI AAT'ATT Cuts at: 5 52 2644 3628 4084 4819 5 Size: 47 2592 984 456 735 2081 Fragments arranged by size: 2592 2081 984 735 456 47 StuI AGG'CCT Cuts at: 1517 3417 1517 Size: 1900 4995 StyI C'CwwG_G Cuts at: 513 1065 2537 2892 3418 4334 513 Size: 552 1472 355 526 916 3074 Fragments arranged by size: 3074 1472 916 552 526 355 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4922 4939 5092 5277 5722 325 Size: 1255 3342 17 153 185 445 1498 Fragments arranged by size: 3342 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5141 6853 96 Size: 274 80 33 51 1443 3164 1712 138 Fragments arranged by size: 3164 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6855 6887 6855 Size: 32 6863 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6895 XbaI T'CTAG_A Cuts at: 4231 4231 Size: 6895 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2698 2698 Size: 6895 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6895 XmaI C'CCGG_G Cuts at: 1870 4220 1870 Size: 2350 4545 XmnI GAAnn'nnTTC Cuts at: 3255 3834 5024 3255 Size: 579 1190 5126 Fragments arranged by size: 5126 1190 579 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4703 276 Size: 53 83 186 936 3169 2468 Fragments arranged by size: 3169 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI