Restriction map for
pFN21AB5144
(Linear) (Six-base) MAPSORT of: KIBB5144.seq Check: 9562 from: 1 to: 2190
KIBB5144.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 1844 2190
Size: 185 1659 346
Fragments arranged by size:
1659 346 185
AflIII A'CryG_T
Cuts at: 0 851 2190
Size: 851 1339
AlwNI CAG_nnn'CTG
Cuts at: 0 1900 2182 2190
Size: 1900 282 8
Fragments arranged by size:
1900 282 8
ApoI r'AATT_y
Cuts at: 0 371 1110 1129 1484 1828 2064 2190
Size: 371 739 19 355 344 236 126
Fragments arranged by size:
739 371 355 344 236 126 19
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 134 167 2190
Size: 134 33 2023
Fragments arranged by size:
2023 134 33
BamHI G'GATC_C
Cuts at: 0 2098 2190
Size: 2098 92
BanII G_rGCy'C
Cuts at: 0 887 2190
Size: 887 1303
BbsI GAAGACnn'nnnn_
Cuts at: 0 86 737 1498 1670 2190
Size: 86 651 761 172 520
Fragments arranged by size:
761 651 520 172 86
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2034 2068 2190
Size: 2034 34 122
Fragments arranged by size:
2034 122 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2106 2190
Size: 2106 84
BclI T'GATC_A
Cuts at: 0 728 2190
Size: 728 1462
BfrBI ATG'CAT
Cuts at: 0 1100 2190
Size: 1100 1090
BlpI GC'TnA_GC
Cuts at: 0 1320 2190
Size: 1320 870
BmrI ACTGGGnnnn_n'
Cuts at: 0 427 1888 2190
Size: 427 1461 302
Fragments arranged by size:
1461 427 302
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 510 901 915 2190
Size: 510 391 14 1275
Fragments arranged by size:
1275 510 391 14
Bpu10I CC'TnA_GC
Cuts at: 0 863 2190
Size: 863 1327
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 896 2190
Size: 896 1294
BsaBI GATnn'nnATC
Cuts at: 0 685 2190
Size: 685 1505
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1721 2190
Size: 1721 469
BseYI C'CCAG_C
Cuts at: 0 1680 1693 2190
Size: 1680 13 497
Fragments arranged by size:
1680 497 13
BsmI GAATG_Cn'
Cuts at: 0 737 814 842 2190
Size: 737 77 28 1348
Fragments arranged by size:
1348 737 77 28
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1111 2190
Size: 1111 1079
Bsp1286I G_dGCh'C
Cuts at: 0 887 2190
Size: 887 1303
BspHI T'CATG_A
Cuts at: 0 1124 2190
Size: 1124 1066
BsrDI GCAATG_nn'
Cuts at: 0 950 1747 2190
Size: 950 797 443
Fragments arranged by size:
950 797 443
BsrFI r'CCGG_y
Cuts at: 0 1315 2190
Size: 1315 875
BstAPI GCAn_nnn'nTGC
Cuts at: 0 528 2190
Size: 528 1662
BstYI r'GATC_y
Cuts at: 0 160 2098 2190
Size: 160 1938 92
Fragments arranged by size:
1938 160 92
Bsu36I CC'TnA_GG
Cuts at: 0 927 2190
Size: 927 1263
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1360 2027 2190
Size: 1360 667 163
Fragments arranged by size:
1360 667 163
BtsI GCAGTG_nn'
Cuts at: 0 1588 2190
Size: 1588 602
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1031 1066 2190
Size: 1031 35 1124
Fragments arranged by size:
1124 1031 35
DraI TTT'AAA
Cuts at: 0 824 2190
Size: 824 1366
EaeI y'GGCC_r
Cuts at: 0 126 2190
Size: 126 2064
EarI CTCTTCn'nnn_
Cuts at: 0 128 1241 1265 1283 2190
Size: 128 1113 24 18 907
Fragments arranged by size:
1113 907 128 24 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 510 901 915 1844 2190
Size: 185 325 391 14 929 346
Fragments arranged by size:
929 391 346 325 185 14
EcoO109I rG'GnC_Cy
Cuts at: 0 1983 2190
Size: 1983 207
EcoRI G'AATT_C
Cuts at: 0 371 2190
Size: 371 1819
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 85 203 1504 2190
Size: 85 118 1301 686
Fragments arranged by size:
1301 686 118 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 152 184 1804 1836 1967 1999 2041 2043
Size: 152 32 1620 32 131 32 42 2
Cuts at: 2043 2073 2075 2190
Size: 30 2 115
Fragments arranged by size:
1620 152 131 115 42 32 32 32
30 2 2
HindIII A'AGCT_T
Cuts at: 0 865 2190
Size: 865 1325
MfeI C'AATT_G
Cuts at: 0 833 2190
Size: 833 1357
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 1867 2190
Size: 204 1663 323
Fragments arranged by size:
1663 323 204
MscI TGG'CCA
Cuts at: 0 128 2190
Size: 128 2062
MslI CAynn'nnrTG
Cuts at: 0 267 995 2190
Size: 267 728 1195
Fragments arranged by size:
1195 728 267
MspA1I CmG'CkG
Cuts at: 0 88 2190
Size: 88 2102
NdeI CA'TA_TG
Cuts at: 0 783 2190
Size: 783 1407
NsiI A_TGCA'T
Cuts at: 0 1102 2190
Size: 1102 1088
NspI r_CATG'y
Cuts at: 0 855 2190
Size: 855 1335
PciI A'CATG_T
Cuts at: 0 851 2190
Size: 851 1339
PflMI CCAn_nnn'nTGG
Cuts at: 0 893 2068 2190
Size: 893 1175 122
Fragments arranged by size:
1175 893 122
PpuMI rG'GwC_Cy
Cuts at: 0 1983 2190
Size: 1983 207
PstI C_TGCA'G
Cuts at: 0 1200 2190
Size: 1200 990
PvuII CAG'CTG
Cuts at: 0 88 2190
Size: 88 2102
SapI GCTCTTCn'nnn_
Cuts at: 0 1265 1283 2190
Size: 1265 18 907
Fragments arranged by size:
1265 907 18
SfcI C'TryA_G
Cuts at: 0 233 1196 1901 2190
Size: 233 963 705 289
Fragments arranged by size:
963 705 289 233
SmlI C'TyrA_G
Cuts at: 0 911 2190
Size: 911 1279
SspI AAT'ATT
Cuts at: 0 639 1623 2079 2190
Size: 639 984 456 111
Fragments arranged by size:
984 639 456 111
StuI AGG'CCT
Cuts at: 0 1412 2190
Size: 1412 778
StyI C'CwwG_G
Cuts at: 0 532 887 1413 2190
Size: 532 355 526 777
Fragments arranged by size:
777 532 526 355
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 693 2190
Size: 693 1497
XmnI GAAnn'nnTTC
Cuts at: 0 1250 1829 2190
Size: 1250 579 361
Fragments arranged by size:
1250 579 361
Enzymes that do cut:
AcuI AflIII AlwNI ApoI BaeI BamHI BanII BbsI
BcgI BciVI BclI BfrBI BlpI BmrI BpmI Bpu10I
BpuEI BsaBI BseRI BseYI BsmI BsmBI Bsp1286I BspHI
BsrDI BsrFI BstAPI BstYI Bsu36I BtgZI BtsI CspCI
DraI EaeI EarI Eco57MI EcoO109I EcoRI FalI Hin4I
HindIII MfeI MmeI MscI MslI MspA1I NdeI NsiI
NspI PciI PflMI PpuMI PstI PvuII SapI SfcI
SmlI SspI StuI StyI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BanI BbeI BbvCI BglI BglII
Bme1580I BmgBI BmtI BplI BsaI BsaAI BsaHI BsaWI
BsaXI BsgI BsiEI BsiHKAI BsiWI BspEI BspMI BsrBI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I BtgI
ClaI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MluI NaeI NarI NcoI NgoMIV NheI NotI NruI
PacI PasI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SwaI TaqII TatI
TstI Tth111I XbaI XhoI XmaI ZraI