(Circular) (Six-base) MAPSORT of: pFN21AB5083.seq Check: 5909 from: 1 to: 6760 pFN21AB5083 6760 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4570 278 Size: 53 83 186 936 3034 2468 Fragments arranged by size: 3034 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2226 2325 2816 2894 4103 1937 Size: 289 99 491 78 1209 4594 Fragments arranged by size: 4594 1209 491 289 99 78 Acc65I G'GTAC_C Cuts at: 2729 3555 4081 2729 Size: 826 526 5408 Fragments arranged by size: 5408 826 526 AclI AA'CG_TT Cuts at: 1563 4059 4887 5260 1563 Size: 2496 828 373 3063 Fragments arranged by size: 3063 2496 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2772 3797 4823 6207 6475 1632 Size: 1140 1025 1026 1384 268 1917 Fragments arranged by size: 1917 1384 1140 1026 1025 268 AfeI AGC'GCT Cuts at: 2246 2246 Size: 6760 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6563 AflIII A'CryG_T Cuts at: 1203 5659 6636 1203 Size: 4456 977 1327 Fragments arranged by size: 4456 1327 977 AgeI A'CCGG_T Cuts at: 5591 5591 Size: 6760 AhdI GACnn_n'nnGTC Cuts at: 3031 5489 3031 Size: 2458 4302 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6760 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2952 1184 Size: 1768 4992 AlwNI CAG_nnn'CTG Cuts at: 2068 3110 4503 6075 2068 Size: 1042 1393 1572 2753 Fragments arranged by size: 2753 1572 1393 1042 ApaI G_GGCC'C Cuts at: 2800 2800 Size: 6760 ApaLI G'TGCA_C Cuts at: 4817 5973 4817 Size: 1156 5604 ApoI r'AATT_y Cuts at: 1493 2409 3088 3874 4069 4295 4447 6556 1493 Size: 916 679 786 195 226 152 2109 1697 Fragments arranged by size: 2109 1697 916 786 679 226 195 152 AseI AT'TA_AT Cuts at: 160 1032 2643 5314 160 Size: 872 1611 2671 1606 Fragments arranged by size: 2671 1611 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6760 AvaI C'yCGr_G Cuts at: 1870 1943 2120 2197 4085 1870 Size: 73 177 77 1888 4545 Fragments arranged by size: 4545 1888 177 77 73 BamHI G'GATC_C Cuts at: 4090 4457 4090 Size: 367 6393 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2729 3555 3860 4081 5536 Size: 325 217 627 942 826 305 221 1455 Cuts at: 5536 618 Size: 1842 Fragments arranged by size: 1842 1455 942 826 627 325 305 221 217 BanII G_rGCy'C Cuts at: 1391 2800 3264 4079 1391 Size: 1409 464 815 4072 Fragments arranged by size: 4072 1409 815 464 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3027 3189 3402 3799 928 Size: 971 1128 162 213 397 3889 Fragments arranged by size: 3889 1128 971 397 213 162 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4951 4985 1903 Size: 34 3014 34 3678 Fragments arranged by size: 3678 3014 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4652 5862 4652 Size: 1210 5550 BclI T'GATC_A Cuts at: 1548 3886 1548 Size: 2338 4422 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5371 136 Size: 107 122 71 631 605 3699 1525 Fragments arranged by size: 3699 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2208 2617 3766 6755 2208 Size: 409 1149 2989 2213 Fragments arranged by size: 2989 2213 1149 409 BlpI GC'TnA_GC Cuts at: 3494 4177 3494 Size: 683 6077 Bme1580I G_kGCm'C Cuts at: 1790 2160 2800 4821 5977 1790 Size: 370 640 2021 1156 2573 Fragments arranged by size: 2573 2021 1156 640 370 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6760 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2247 2967 4491 Size: 416 53 83 399 24 830 720 1524 Cuts at: 4491 5449 442 Size: 958 1753 Fragments arranged by size: 1753 1524 958 830 720 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6760 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3248 5420 1239 Size: 2009 2172 2579 Fragments arranged by size: 2579 2172 2009 Bpu10I CC'TnA_GC Cuts at: 3197 3690 3197 Size: 493 6267 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4885 5750 6048 4885 Size: 865 298 5597 Fragments arranged by size: 5597 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5423 882 Size: 617 3924 2219 Fragments arranged by size: 3924 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4551 493 Size: 711 3347 2702 Fragments arranged by size: 3347 2702 711 BsaBI GATnn'nnATC Cuts at: 6754 6754 Size: 6760 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4567 4949 5584 275 Size: 53 83 186 936 3034 382 635 1451 Fragments arranged by size: 3034 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3022 5193 5591 5865 6012 6423 3022 Size: 2171 398 274 147 411 3359 Fragments arranged by size: 3359 2171 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3670 3700 1178 Size: 30 165 30 2267 30 4238 Fragments arranged by size: 4238 2267 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3272 3758 1186 Size: 2086 486 4188 Fragments arranged by size: 4188 2086 486 BseYI C'CCAG_C Cuts at: 2068 2266 3079 3236 3673 5963 6459 2068 Size: 198 813 157 437 2290 496 2369 Fragments arranged by size: 2369 2290 813 496 437 198 157 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2453 1163 Size: 573 717 5470 Fragments arranged by size: 5470 717 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2108 4210 4971 5999 1540 Size: 275 186 107 2102 761 1028 2301 Fragments arranged by size: 2301 2102 1028 761 275 186 107 BsiHKAI G_wGCw'C Cuts at: 3264 3500 3604 4821 4906 5977 3264 Size: 236 104 1217 85 1071 4047 Fragments arranged by size: 4047 1217 1071 236 104 85 BsmI GAATG_Cn' Cuts at: 3392 3477 4271 4364 3392 Size: 85 794 93 5788 Fragments arranged by size: 5788 794 93 85 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2160 2800 3264 3500 3604 4079 4821 Size: 399 370 640 464 236 104 475 742 Cuts at: 4821 4906 5977 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 640 475 464 399 370 236 104 85 BspEI T'CCGG_A Cuts at: 3022 6423 3022 Size: 3401 3359 BspHI T'CATG_A Cuts at: 2593 4649 6379 2593 Size: 2056 1730 2974 Fragments arranged by size: 2974 2056 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4115 844 Size: 3271 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4647 1394 Size: 429 2824 3507 Fragments arranged by size: 3507 2824 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2909 5255 5429 65 Size: 1183 1661 2346 174 1396 Fragments arranged by size: 2346 1661 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5404 5591 1700 Size: 110 3594 187 2869 Fragments arranged by size: 3594 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6760 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6760 BssSI C'ACGA_G Cuts at: 4820 5832 4820 Size: 1012 5748 BstAPI GCAn_nnn'nTGC Cuts at: 794 2079 2431 3110 3387 3683 794 Size: 1285 352 679 277 296 3871 Fragments arranged by size: 3871 1285 679 352 296 277 BstBI TT'CG_AA Cuts at: 3790 5564 3790 Size: 1774 4986 BstEII G'GTnAC_C Cuts at: 1184 3514 1184 Size: 2330 4430 BstYI r'GATC_y Cuts at: 1972 2208 2617 3766 4090 4457 4842 4859 6311 Size: 236 409 1149 324 367 385 17 1452 Cuts at: 6311 6397 6409 6755 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1149 409 385 367 346 324 236 86 17 12 BstZ17I GTA'TAC Cuts at: 2817 2895 2817 Size: 78 6682 Bsu36I CC'TnA_GG Cuts at: 2561 2561 Size: 6760 BtgI C'CryG_G Cuts at: 513 1065 2004 2652 3405 513 Size: 552 939 648 753 3868 Fragments arranged by size: 3868 939 753 648 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6021 Fragments arranged by size: 6021 683 56 BtsI GCAGTG_nn' Cuts at: 3388 4276 5070 5090 3388 Size: 888 794 20 5058 Fragments arranged by size: 5058 888 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6760 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3265 3300 529 Size: 35 2701 35 3989 Fragments arranged by size: 3989 2701 35 35 DraI TTT'AAA Cuts at: 4064 4064 Size: 6760 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3575 5767 809 Size: 507 2259 2192 1802 Fragments arranged by size: 2259 2192 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2105 3655 4207 Size: 54 1425 50 275 18 275 1550 552 Cuts at: 4207 5096 6725 8 Size: 889 1629 43 Fragments arranged by size: 1629 1550 1425 889 552 275 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2105 4207 1537 Size: 275 293 2102 4090 Fragments arranged by size: 4090 2102 293 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4690 1353 Size: 3337 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5347 5721 5867 387 Size: 1064 138 3758 374 146 1280 Fragments arranged by size: 3758 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3262 3262 Size: 6760 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2772 3248 3797 4823 5420 6207 6475 Size: 393 1140 476 549 1026 597 787 268 Cuts at: 6475 1239 Size: 1524 Fragments arranged by size: 1524 1140 1026 787 597 549 476 393 268 EcoO109I rG'GnC_Cy Cuts at: 2797 2835 3835 2797 Size: 38 1000 5722 Fragments arranged by size: 5722 1000 38 EcoRI G'AATT_C Cuts at: 2409 4069 4447 2409 Size: 1660 378 4722 Fragments arranged by size: 4722 1660 378 EcoRV GAT'ATC Cuts at: 3957 3957 Size: 6760 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2296 3109 3944 2296 Size: 813 835 5112 Fragments arranged by size: 5112 835 813 FspI TGC'GCA Cuts at: 5266 5266 Size: 6760 HaeII r_GCGC'y Cuts at: 1678 2069 2248 5907 1678 Size: 391 179 3659 2531 Fragments arranged by size: 3659 2531 391 179 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2544 2576 3017 3049 3786 Size: 32 135 32 972 32 441 32 737 Cuts at: 3786 3818 3941 3973 5401 5433 5475 5507 1373 Size: 32 123 32 1428 32 42 32 2626 Fragments arranged by size: 2626 1428 972 737 441 135 123 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2227 2535 4104 4350 669 Size: 988 281 289 308 1569 246 3079 Fragments arranged by size: 3079 1569 988 308 289 281 246 HindIII A'AGCT_T Cuts at: 748 3194 748 Size: 2446 4314 HpaI GTT'AAC Cuts at: 4350 4350 Size: 6760 KpnI G_GTAC'C Cuts at: 2733 3559 4085 2733 Size: 826 526 5408 Fragments arranged by size: 5408 826 526 MfeI C'AATT_G Cuts at: 4359 4359 Size: 6760 MluI A'CGCG_T Cuts at: 6636 6636 Size: 6760 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5874 6058 1305 Size: 4569 184 2007 Fragments arranged by size: 4569 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3657 10 Size: 54 1425 343 1825 3113 Fragments arranged by size: 3113 1825 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2051 2424 2778 3508 4718 5077 5236 Size: 648 885 373 354 730 1210 359 159 Cuts at: 5236 518 Size: 2042 Fragments arranged by size: 2042 1210 885 730 648 373 359 354 159 MspA1I CmG'CkG Cuts at: 1606 3107 3369 3493 4176 4853 6001 6246 1606 Size: 1501 262 124 683 677 1148 245 2120 Fragments arranged by size: 2120 1501 1148 683 677 262 245 124 NcoI C'CATG_G Cuts at: 513 1065 2004 2652 3405 513 Size: 552 939 648 753 3868 Fragments arranged by size: 3868 939 753 648 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6760 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6760 NotI GC'GGCC_GC Cuts at: 2105 4207 2105 Size: 2102 4658 NspI r_CATG'y Cuts at: 1132 1229 2429 3463 4042 4118 5663 1132 Size: 97 1200 1034 579 76 1545 2229 Fragments arranged by size: 2229 1545 1200 1034 579 97 76 PasI CC'CwG_GG Cuts at: 1348 2800 1348 Size: 1452 5308 PciI A'CATG_T Cuts at: 5659 5659 Size: 6760 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6760 PmeI GTTT'AAAC Cuts at: 4064 4064 Size: 6760 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4829 4861 6342 6374 4829 Size: 32 1481 32 5215 Fragments arranged by size: 5215 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2676 1613 Size: 1063 5697 PsiI TTA'TAA Cuts at: 4330 4330 Size: 6760 PspOMI G'GGCC_C Cuts at: 2796 2796 Size: 6760 PstI C_TGCA'G Cuts at: 830 2492 4112 830 Size: 1662 1620 3478 Fragments arranged by size: 3478 1662 1620 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6760 PvuII CAG'CTG Cuts at: 3107 3369 3493 3107 Size: 262 124 6374 Fragments arranged by size: 6374 262 124 SacI G_AGCT'C Cuts at: 3264 3264 Size: 6760 SalI G'TCGA_C Cuts at: 1936 2225 4102 1936 Size: 289 1877 4594 Fragments arranged by size: 4594 1877 289 SbfI CC_TGCA'GG Cuts at: 4112 4112 Size: 6760 ScaI AGT'ACT Cuts at: 5008 6720 5008 Size: 1712 5048 SfcI C'TryA_G Cuts at: 826 1045 2488 3370 3907 4108 5243 5924 6115 Size: 219 1443 882 537 201 1135 681 191 Cuts at: 6115 826 Size: 1471 Fragments arranged by size: 1471 1443 1135 882 681 537 219 201 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6760 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6760 SmaI CCC'GGG Cuts at: 1872 4087 1872 Size: 2215 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4864 5765 6027 820 Size: 197 926 2921 901 262 1553 Fragments arranged by size: 2921 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4551 493 Size: 4058 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6760 SphI G_CATG'C Cuts at: 1132 4118 1132 Size: 2986 3774 SspI AAT'ATT Cuts at: 5 52 4684 5 Size: 47 4632 2081 Fragments arranged by size: 4632 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6760 StyI C'CwwG_G Cuts at: 513 1065 2004 2652 2700 3405 4199 513 Size: 552 939 648 48 705 794 3074 Fragments arranged by size: 3074 939 794 705 648 552 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2666 4787 4804 4957 5142 5587 325 Size: 1255 1086 2121 17 153 185 445 1498 Fragments arranged by size: 2121 1498 1255 1086 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5006 6718 96 Size: 274 80 33 51 1443 3029 1712 138 Fragments arranged by size: 3029 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6720 6752 6720 Size: 32 6728 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6760 XbaI T'CTAG_A Cuts at: 4096 4096 Size: 6760 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6760 XmaI C'CCGG_G Cuts at: 1870 4085 1870 Size: 2215 4545 XmnI GAAnn'nnTTC Cuts at: 3927 4889 3927 Size: 962 5798 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4568 276 Size: 53 83 186 936 3034 2468 Fragments arranged by size: 3034 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI