Restriction map for
pFN21AB5025
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BamHI BbrPI
BbvCI BciVI BfaI BfrI BglI
BglII BlnI BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmFI BsmI
Bsp1407I BspEI BspHI BspLU11I BspMI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HincII
HindII HinfI HpaI HphI KpnI
MaeI MaeII MaeIII MamI MfeI
MflI MluI MroI MseI MslI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SalI SbfI ScaI SexAI SfaNI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp45I
Tsp509I Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AcyI 88 GGCGCC G[GA]^CG[CT]C
ApaI 368 GGGCCC GGGCC^C
BalI 2 TGGCCA TGG^CCA
BbeI 88 GGCGCC GGCGC^C
BbsI 229 GTCTTC GAAGAC(2/6)
BcgI 198 CGAGGGCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 401 TGATCA T^GATCA
BpuAI 229 GTCTTC GAAGAC(2/6)
BsaHI 88 GGCGCC G[GA]^CG[CT]C
BspDI 532 ATCGAT AT^CGAT
BsrBI 643 GAGCGG CCGCTC(-3/-3)
ClaI 532 ATCGAT AT^CGAT
EarI 393 GAAGAG CTCTTC(1/4)
EcoNI 270 CCTCATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 581 CCAAGG C^C[AT][AT]GG
FbaI 401 TGATCA T^GATCA
Hin1I 88 GGCGCC G[GA]^CG[CT]C
HindIII 520 AAGCTT A^AGCTT
KasI 88 GGCGCC G^GCGCC
Ksp632I 393 GAAGAG CTCTTC(1/4)
KspI 473 CCGCGG CCGC^GG
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
NarI 88 GGCGCC GG^CGCC
NspI 290 GCATGT [GA]CATG^[CT]
PpuMI 278 AGGACCT [GA]G^G[AT]CC[CT]
SacII 473 CCGCGG CCGC^GG
SapI 393 GAAGAGC GCTCTTC(1/4)
SfoI 88 GGCGCC GGC^GCC
SmlI 697 CTCAAG C^T[CT][GA]AG
StyI 581 CCAAGG C^C[AT][AT]GG
XcmI 5 CCAGTGAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AvaI 112 CCCGAG C^[CT]CG[GA]G
118 CCCGAG
AvaII 279 GGACC G^G[AT]CC
585 GGTCC
BanI 88 GGCGCC G^G[CT][GA]CC
559 GGTGCC
BsoBI 112 CCCGAG C^[CT]CG[GA]G
118 CCCGAG
BsrFI 35 GCCGGC [GA]^CCGG[CT]
179 GCCGGC
BsrI 5 CCAGT ACTGG(1/-1)
605 CCAGT
BstF5I 318 GGATG GGATG(2/0)
414 GGATG
Cfr10I 35 GCCGGC [GA]^CCGG[CT]
179 GCCGGC
DpnII 402 GATC ^GATC
531 GATC
DsaI 140 CCACGG C^C[GA][CT]GG
473 CCGCGG
FokI 318 GGATG GGATG(9/13)
414 GGATG
HgaI 154 GACGC GACGC(5/10)
307 GACGC
MboI 402 GATC ^GATC
531 GATC
MboII 393 GAAGA GAAGA(8/7)
230 TCTTC
NaeI 35 GCCGGC GCC^GGC
179 GCCGGC
NdeII 402 GATC ^GATC
531 GATC
NgoMIV 35 GCCGGC G^CCGGC
179 GCCGGC
NlaIII 291 CATG CATG^
399 CATG
PstI 222 CTGCAG CTGCA^G
487 CTGCAG
PvuII 73 CAGCTG CAG^CTG
172 CAGCTG
Sau3AI 402 GATC ^GATC
531 GATC
SfcI 222 CTGCAG C^T[GA][CT]AG
487 CTGCAG
TspRI 6 CAGTG CA[GC]TG[ACGT][ACGT]^
498 CAGTG
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AccII AciI AluI BanII BbvI
BcnI BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsiYI BslI Bsp1286I
BssKI BstNI BstUI Cac8I CelII
CfoI Cfr13I DdeI DraII EaeI
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HpaII
ItaI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIV Sau96I
ScrFI TaqI TseI TthHB8I