(Circular) (Six-base) MAPSORT of: pFN21AB5025.seq Check: 6623 from: 1 to: 5422 pFN21AB5025 5422 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3232 278 Size: 53 83 186 936 1696 2468 Fragments arranged by size: 2468 1696 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2765 1937 Size: 828 4594 Acc65I G'GTAC_C Cuts at: 2743 2743 Size: 5422 AclI AA'CG_TT Cuts at: 1563 3549 3922 1563 Size: 1986 373 3063 Fragments arranged by size: 3063 1986 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2369 2417 3485 4869 5137 1632 Size: 737 48 1068 1384 268 1917 Fragments arranged by size: 1917 1384 1068 737 268 48 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5225 AflIII A'CryG_T Cuts at: 1203 4321 5298 1203 Size: 3118 977 1327 Fragments arranged by size: 3118 1327 977 AgeI A'CCGG_T Cuts at: 4253 4253 Size: 5422 AhdI GACnn_n'nnGTC Cuts at: 4151 4151 Size: 5422 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5422 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5422 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2282 2314 2282 Size: 32 5390 AlwNI CAG_nnn'CTG Cuts at: 3165 4737 3165 Size: 1572 3850 ApaI G_GGCC'C Cuts at: 2377 2377 Size: 5422 ApaLI G'TGCA_C Cuts at: 3479 4635 3479 Size: 1156 4266 ApoI r'AATT_y Cuts at: 1493 2731 2957 3109 5218 1493 Size: 1238 226 152 2109 1697 Fragments arranged by size: 2109 1697 1238 226 152 AseI AT'TA_AT Cuts at: 160 1032 3976 160 Size: 872 2944 1606 Fragments arranged by size: 2944 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5422 AvaI C'yCGr_G Cuts at: 1870 1943 2117 2123 2747 1870 Size: 73 174 6 624 4545 Fragments arranged by size: 4545 624 174 73 6 BamHI G'GATC_C Cuts at: 2752 3119 2752 Size: 367 5055 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2093 2564 2743 4198 618 Size: 325 217 627 306 471 179 1455 1842 Fragments arranged by size: 1842 1455 627 471 325 306 217 179 BanII G_rGCy'C Cuts at: 1391 2112 2124 2377 2741 1391 Size: 721 12 253 364 4072 Fragments arranged by size: 4072 721 364 253 12 BbeI G_GCGC'C Cuts at: 2097 2097 Size: 5422 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2227 928 Size: 971 328 4123 Fragments arranged by size: 4123 971 328 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2192 2226 3613 3647 1903 Size: 34 255 34 1387 34 3678 Fragments arranged by size: 3678 1387 255 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3314 4524 3314 Size: 1210 4212 BclI T'GATC_A Cuts at: 1548 2406 1548 Size: 858 4564 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4033 136 Size: 107 122 71 631 605 2361 1525 Fragments arranged by size: 2361 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5417 5417 Size: 5422 BlpI GC'TnA_GC Cuts at: 2168 2180 2441 2637 2839 2168 Size: 12 261 196 202 4751 Fragments arranged by size: 4751 261 202 196 12 Bme1580I G_kGCm'C Cuts at: 1790 2377 3483 4639 1790 Size: 587 1106 1156 2573 Fragments arranged by size: 2573 1156 1106 587 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5422 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3153 4111 442 Size: 416 53 83 399 24 1736 958 1753 Fragments arranged by size: 1753 1736 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5422 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 2309 2570 4082 1239 Size: 815 255 261 1512 2579 Fragments arranged by size: 2579 1512 815 261 255 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2687 3547 4412 4710 2687 Size: 860 865 298 3399 Fragments arranged by size: 3399 865 860 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4085 882 Size: 617 2586 2219 Fragments arranged by size: 2586 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3213 493 Size: 711 2009 2702 Fragments arranged by size: 2702 2009 711 BsaBI GATnn'nnATC Cuts at: 5416 5416 Size: 5422 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2094 3229 3611 4246 Size: 53 83 186 936 561 1135 382 635 Cuts at: 4246 275 Size: 1451 Fragments arranged by size: 1451 1135 936 635 561 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3855 4253 4527 4674 5085 3855 Size: 398 274 147 411 4192 Fragments arranged by size: 4192 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2009 2039 2282 2312 1178 Size: 30 165 30 606 30 243 30 4288 Fragments arranged by size: 4288 606 243 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 2069 2078 2675 2678 2681 1186 Size: 844 39 9 597 3 3 3927 Fragments arranged by size: 3927 844 597 39 9 3 3 BseYI C'CCAG_C Cuts at: 2076 2098 2175 2315 2368 4625 5121 2076 Size: 22 77 140 53 2257 496 2377 Fragments arranged by size: 2377 2257 496 140 77 53 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4849 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2872 3633 4661 1540 Size: 275 186 871 761 1028 2301 Fragments arranged by size: 2301 1028 871 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3483 3568 4639 3483 Size: 85 1071 4266 Fragments arranged by size: 4266 1071 85 BsmI GAATG_Cn' Cuts at: 2933 3026 2933 Size: 93 5329 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2112 2124 2377 2741 3483 3568 4639 Size: 399 322 12 253 364 742 85 1071 Cuts at: 4639 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 364 322 253 85 12 BspEI T'CCGG_A Cuts at: 5085 5085 Size: 5422 BspHI T'CATG_A Cuts at: 3311 5041 3311 Size: 1730 3692 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2777 844 Size: 1933 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2650 3309 1394 Size: 429 827 659 3507 Fragments arranged by size: 3507 827 659 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3917 4091 65 Size: 1183 2669 174 1396 Fragments arranged by size: 2669 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2040 2184 4066 4253 1700 Size: 110 230 144 1882 187 2869 Fragments arranged by size: 2869 1882 230 187 144 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5422 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5422 BssSI C'ACGA_G Cuts at: 3482 4494 3482 Size: 1012 4410 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5422 BstBI TT'CG_AA Cuts at: 4226 4226 Size: 5422 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5422 BstYI r'GATC_y Cuts at: 1972 2752 3119 3504 3521 4973 5059 5071 5417 Size: 780 367 385 17 1452 86 12 346 Cuts at: 5417 1972 Size: 1977 Fragments arranged by size: 1977 1452 780 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2145 2478 513 Size: 552 939 141 333 3457 Fragments arranged by size: 3457 939 552 333 141 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4683 Fragments arranged by size: 4683 683 56 BtsI GCAGTG_nn' Cuts at: 2938 3732 3752 2938 Size: 794 20 4608 Fragments arranged by size: 4608 794 20 ClaI AT'CG_AT Cuts at: 1554 2538 1554 Size: 984 4438 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5387 DraI TTT'AAA Cuts at: 2726 2726 Size: 5422 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4429 809 Size: 507 3113 1802 Fragments arranged by size: 3113 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2148 2607 Size: 54 1425 50 275 18 177 141 459 Cuts at: 2607 2869 3758 5387 8 Size: 262 889 1629 43 Fragments arranged by size: 1629 1425 889 459 275 262 177 141 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2869 1537 Size: 275 1057 4090 Fragments arranged by size: 4090 1057 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2393 3352 1353 Size: 1040 959 3423 Fragments arranged by size: 3423 1040 959 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2669 4009 4383 4529 387 Size: 1064 138 1080 1340 374 146 1280 Fragments arranged by size: 1340 1280 1080 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2309 2369 2417 2570 3485 4082 Size: 393 422 255 60 48 153 915 597 Cuts at: 4082 4869 5137 1239 Size: 787 268 1524 Fragments arranged by size: 1524 915 787 597 422 393 268 255 153 60 48 EcoNI CCTnn'n_nnAGG Cuts at: 2279 2279 Size: 5422 EcoO109I rG'GnC_Cy Cuts at: 2284 2373 2374 2284 Size: 89 1 5332 Fragments arranged by size: 5332 89 1 EcoRI G'AATT_C Cuts at: 2731 3109 2731 Size: 378 5044 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2461 2593 2728 2461 Size: 132 135 5155 Fragments arranged by size: 5155 135 132 FspI TGC'GCA Cuts at: 3928 3928 Size: 5422 HaeII r_GCGC'y Cuts at: 1678 2041 2097 2157 2175 2211 2227 2519 4569 Size: 363 56 60 18 36 16 292 2050 Cuts at: 4569 1678 Size: 2531 Fragments arranged by size: 2531 2050 363 292 60 56 36 18 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4063 4095 4137 4169 1373 Size: 32 135 32 2491 32 42 32 2626 Fragments arranged by size: 2626 2491 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2766 3012 669 Size: 988 281 828 246 3079 Fragments arranged by size: 3079 988 828 281 246 HindIII A'AGCT_T Cuts at: 748 2525 748 Size: 1777 3645 HpaI GTT'AAC Cuts at: 3012 3012 Size: 5422 KasI G'GCGC_C Cuts at: 2093 2093 Size: 5422 KpnI G_GTAC'C Cuts at: 2747 2747 Size: 5422 MfeI C'AATT_G Cuts at: 3021 3021 Size: 5422 MluI A'CGCG_T Cuts at: 5298 5298 Size: 5422 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4536 4720 1305 Size: 3231 184 2007 Fragments arranged by size: 3231 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 3423 Fragments arranged by size: 3423 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3380 3739 3898 518 Size: 648 2214 359 159 2042 Fragments arranged by size: 2214 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2080 2179 2480 2838 3515 4663 4908 1606 Size: 474 99 301 358 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 474 358 301 245 99 NaeI GCC'GGC Cuts at: 2042 2186 2042 Size: 144 5278 NarI GG'CG_CC Cuts at: 2094 2094 Size: 5422 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3931 Fragments arranged by size: 3931 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5422 NgoMIV G'CCGG_C Cuts at: 2040 2184 2040 Size: 144 5278 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5422 NotI GC'GGCC_GC Cuts at: 2869 2869 Size: 5422 NspI r_CATG'y Cuts at: 1132 1229 2299 2780 4325 1132 Size: 97 1070 481 1545 2229 Fragments arranged by size: 2229 1545 1070 481 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5422 PciI A'CATG_T Cuts at: 4321 4321 Size: 5422 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5422 PfoI T'CCnGG_A Cuts at: 2256 2448 2256 Size: 192 5230 PmeI GTTT'AAAC Cuts at: 2726 2726 Size: 5422 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2282 2314 3491 3523 5004 5036 2282 Size: 32 1177 32 1481 32 2668 Fragments arranged by size: 2668 1481 1177 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2284 2284 Size: 5422 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5422 PsiI TTA'TAA Cuts at: 2992 2992 Size: 5422 PspOMI G'GGCC_C Cuts at: 2373 2373 Size: 5422 PstI C_TGCA'G Cuts at: 830 2231 2496 2774 830 Size: 1401 265 278 3478 Fragments arranged by size: 3478 1401 278 265 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5422 PvuII CAG'CTG Cuts at: 2080 2179 2080 Size: 99 5323 SacII CC_GC'GG Cuts at: 2481 2481 Size: 5422 SalI G'TCGA_C Cuts at: 1936 2764 1936 Size: 828 4594 SapI GCTCTTCn'nnn_ Cuts at: 2393 2393 Size: 5422 SbfI CC_TGCA'GG Cuts at: 2774 2774 Size: 5422 ScaI AGT'ACT Cuts at: 3670 5382 3670 Size: 1712 3710 SfcI C'TryA_G Cuts at: 826 1045 2227 2492 2770 3905 4586 4777 826 Size: 219 1182 265 278 1135 681 191 1471 Fragments arranged by size: 1471 1182 1135 681 278 265 219 191 SfoI GGC'GCC Cuts at: 2095 2095 Size: 5422 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5422 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5422 SmaI CCC'GGG Cuts at: 1872 2749 1872 Size: 877 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2702 3526 4427 4689 820 Size: 197 926 759 824 901 262 1553 Fragments arranged by size: 1553 926 901 824 759 262 197 SnaBI TAC'GTA Cuts at: 493 3213 493 Size: 2720 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5422 SphI G_CATG'C Cuts at: 1132 2780 1132 Size: 1648 3774 SspI AAT'ATT Cuts at: 5 52 3346 5 Size: 47 3294 2081 Fragments arranged by size: 3294 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5422 StyI C'CwwG_G Cuts at: 513 1065 2004 2586 2861 513 Size: 552 939 582 275 3074 Fragments arranged by size: 3074 939 582 552 275 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3449 3466 3619 3804 4249 325 Size: 1255 1869 17 153 185 445 1498 Fragments arranged by size: 1869 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3668 5380 96 Size: 274 80 33 51 1443 1691 1712 138 Fragments arranged by size: 1712 1691 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2009 2041 5382 5414 2009 Size: 32 3341 32 2017 Fragments arranged by size: 3341 2017 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5422 XbaI T'CTAG_A Cuts at: 2758 2758 Size: 5422 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2017 2017 Size: 5422 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5422 XmaI C'CCGG_G Cuts at: 1870 2747 1870 Size: 877 4545 XmnI GAAnn'nnTTC Cuts at: 3551 3551 Size: 5422 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3230 276 Size: 53 83 186 936 1696 2468 Fragments arranged by size: 2468 1696 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SexAI SfiI SrfI SwaI